Detection of Invasive Lionfish (Pterois volitans and Pterois miles) in the Gulf of Mexico Using Environmental DNA Methods

Red Lionfish (Pterois volitans) and Devil Firefish (Pterois miles) are Indo-Pacific species introduced into the western North Atlantic Ocean in the 1980s. Their range currently extends from New York to Florida and adjacent waters of the Caribbean Sea and the Gulf of Mexico. Since the original sighting in 2010 off the Texas coast lionfish populations in the Gulf of Mexico have increased substantially and negative ecological impacts are expected. Lionfish detection relies on underwater visual surveys using divers and ROVs which are expensive and not always effective. This study seeks to employ real time polymerase chain reaction (RT PCR) to amplify aqueous environmental DNA (eDNA) as a highly sensitive alternative to detect and quantify lionfish. To minimize false positives and false negatives, highly specific primer sets targeting the mitochondrial DNA genome of lionfish were designed using ecoPrimers and ecoPCR. RT PCR on serial DNA dilutions indicated that the D-loop region was the best locus to amplify and quantify eDNA. Lionfish eDNA was successfully quantified in a series of mesocosms differing in volume at Moody Gardens Aquarium. Finally, the Red Lionfish presence was detected by assaying water samples collected from artificial reefs and the Flower Gardens Banks National Marine Sanctuary and adjacent reefs in the Gulf of Mexico

Focused Group Interviews as an Innovative Quanti- Qualitative Methodology (QQM ): Integrating Quantitative Elements into a Qualitative Methodology

There is a sharp divide between quantitative and qualitative methodologies in the social sciences. We investigate an innovative way to bridge this gap that incorporates quantitative techniques into a qualitative method, the “quanti-qualitative method” (QQM). Specifically, our research utilized small survey questionnaires and experiment-like activities as part of the question rout e in a series of five focused group interviews on nutrition education. We show how these quantitative-type activities fit naturally with our question route and contributed to testing the hypotheses within the context of the five important characteristics of focused group interviews. The innovative use of QQM in focused group interviews makes data analysis easier and more transparent and permits collection of richer, more multifaceted data in a cost-effective fashion

Providing Hope after Trauma: Educating in a Juvenile Residential Center

The field of integrated language arts is an ideal forum for sharing stories, discussing perspectives, expressing emotions in a healthy way, and challenging the systems that govern and shape our lives. Accomplishing this goal in a traditional classroom can sometimes be difficult, but for a moment, consider the physical space of a classroom within a juvenile residential center (JRC). This space brings many obstacles that traditional classrooms, teachers, and students do not have to address. To thrive, students need to be in a safe environment of trust. Trust is both critical and challenging to build in a space with so many limitations. By centering student agency, identity, and awareness of structural barriers, teachers may be able to make a positive difference in the lives of incarcerated students, especially those impacted by trauma. This could help students gain a new perspective on their own recidivism and cycles underneath a systemic context, hopefully forging a path toward freedom and healing. This qualitative case study focuses on two novice teachers’ journeys as they navigate their instructional decisions and practice within a JRC

Quantum well behavior of single stacking fault 3C inclusions in 4H-SiC p-i-n diodes studied by ballistic electron emission microscopy

We show that "single" stacking fault 3C inclusions formed in 4H-SiC p-i-n diodes behave as electron quantum wells (QWs) with the QW energy depth of ???0.25 eV below 4H-SiC conduction band minimum, by measuring the Schottky barriers on and away from inclusions with ballistic electron emission microscopy (BEEM). The Schottky barrier on the 4H area ([11-20] oriented) is measured to be essentially the same as (0001) plane studied previously, indicating that the interface pinning effects on both crystal faces are almost identical. Additionally, BEEM current amplitude is observed to be very sensitive to subsurface damage induced by polishing.open91

Uncovering Dangerous Cheats: How Do Avian Hosts Recognize Adult Brood Parasites?

BACKGROUND: Co-evolutionary struggles between dangerous enemies (e.g., brood parasites) and their victims (hosts) lead to the emergence of sophisticated adaptations and counter-adaptations. Salient host tricks to reduce parasitism costs include, as front line defence, adult enemy discrimination. In contrast to the well studied egg stage, investigations addressing the specific cues for adult enemy recognition are rare. Previous studies have suggested barred underparts and yellow eyes may provide cues for the recognition of cuckoos Cuculus canorus by their hosts; however, no study to date has examined the role of the two cues simultaneously under a consistent experimental paradigm. METHODOLOGY/PRINCIPAL FINDINGS: We modify and extend previous work using a novel experimental approach--custom-made dummies with various combinations of hypothesized recognition cues. The salient recognition cue turned out to be the yellow eye. Barred underparts, the only trait examined previously, had a statistically significant but small effect on host aggression highlighting the importance of effect size vs. statistical significance. CONCLUSION: Relative importance of eye vs. underpart phenotypes may reflect ecological context of host-parasite interaction: yellow eyes are conspicuous from the typical direction of host arrival (from above), whereas barred underparts are poorly visible (being visually blocked by the upper part of the cuckoo's body). This visual constraint may reduce usefulness of barred underparts as a reliable recognition cue under a typical situation near host nests. We propose a novel hypothesis that recognition cues for enemy detection can vary in a context-dependent manner (e.g., depending on whether the enemy is approached from below or from above). Further we suggest a particular cue can trigger fear reactions (escape) in some hosts/populations whereas the same cue can trigger aggression (attack) in other hosts/populations depending on presence/absence of dangerous enemies that are phenotypically similar to brood parasites and costs and benefits associated with particular host responses

Egg Eviction Imposes a Recoverable Cost of Virulence in Chicks of a Brood Parasite

Background: Chicks of virulent brood parasitic birds eliminate their nestmates and avoid costly competition for foster parental care. Yet, efforts to evict nest contents by the blind and naked common cuckoo Cuculus canorus hatchling are counterintuitive as both adult parasites and large older cuckoo chicks appear to be better suited to tossing the eggs and young of the foster parents. Methodology/Principal Findings: Here we show experimentally that egg tossing imposed a recoverable growth cost of mass gain in common cuckoo chicks during the nestling period in nests of great reed warbler Acrocephalus arundinaceus hosts. Growth rates of skeletal traits and morphological variables involved in the solicitation of foster parental care remained similar between evictor and non-evictor chicks throughout development. We also detected no increase in predation rates for evicting nests, suggesting that egg tossing behavior by common cuckoo hatchlings does not increase the conspicuousness of nests. Conclusion: The temporary growth cost of egg eviction by common cuckoo hatchlings is the result of constraints imposed by rejecter host adults and competitive nestmates on the timing and mechanism of parasite virulence.Michael G. Anderson, Csaba Moskát, Miklós Bán, Tomáš Grim, Phillip Cassey and Mark E. Haube

Genome sequence of an Enterobacter helveticus strain, 1159/04 (= LMG 23733), isolated from fruit powder

We report the draft genome sequence of Enterobacter helveticus strain LMG 23733, isolated from fruit powder. The draft genome assembly for E. helveticus strain LMG 23733 has a size of 4,635,476 bp and a G+C content of 55.9%