A search for double beta decays of tin isotopes with enhanced sensitivity

A search for the various double beta decay modes of 124Sn and 112Sn has been performed on 75 kg.days of data. New half-life limits for excited states in 124Sn have been obtained including a lower limit for the decay into the first excited 2+ state of 124Te of T_half > 0.87e20 yrs (90% CL) and into the first excited 0+ state of T_half > 1.08e20 yrs (90% CL). Ground state and excited state transitions of 112Sn have also been experimentally explored. A limit for the 2 neutrino double electron capture of T_half > 1.8e19 yrs (90% CL) is obtained. The non-observation of de-excitation gammas from the 0+ at 1888.5keV results in a lower half-life limit on the 0 neutrino double electron capture decay of 112Sn of T_half > 0.8e19 yrs (90% CL), despite a possible resonant enhancement of the decay rate due to degenerated states.Comment: 6 pages, 7 figures, updated analysis and tex

Certified reference materials for radionuclides in Bikini Atoll sediment (IAEA-410) and Pacific Ocean sediment (IAEA-412)

The preparation and characterization of certified reference materials (CRMs) for radionuclide content in sediments collected offshore of Bikini Atoll (IAEA-410) and in the open northwest Pacific Ocean (IAEA-412) are described and the results of the certification process are presented. The certified radionuclides include: 40K, 210Pb (210Po), 226Ra, 228Ra, 228Th, 232Th, 234U, 238U, 239Pu, 239+240Pu and 241Am for IAEA-410 and 40K, 137Cs, 210Pb (210Po), 226Ra, 228Ra, 228Th, 232Th, 235U, 238U, 239Pu, 240Pu and 239+240Pu for IAEA-412. The CRMs can be used for quality assurance and quality control purposes in the analysis of radionuclides in sediments, for development and validation of analytical methods and for staff training

Improvement of Sec-dependent secretion of a heterologous model protein in Bacillus subtilis by saturation mutagenesis of the N-domain of the AmyE signal peptide

Due to the lack of an outer membrane, Gram-positive bacteria (e.g., Bacillus species) are considered as promising host organisms for the secretory production of biotechnologically relevant heterologous proteins. However, the yields of the desired target proteins were often reported to be disappointingly low. Here, we used saturation mutagenesis of the positively charged N-domain (positions 2-7) of the signal peptide of the Bacillus subtilis alpha-amylase (AmyE) as a novel approach for the improvement of the secretion of a heterologous model protein, cutinase from Fusarium solani pisi, by the general secretory pathway of B. subtilis. Automated high-throughput screening of the resulting signal peptide libraries allowed for the identification of four single point mutations that resulted in significantly increased cutinase amounts, three of which surprisingly reduced the net charge of the N-domain from +3 to +2. Characterization of the effects of the identified mutations on protein synthesis and export kinetics by pulse-chase analyses indicates that an optimal balance between biosynthesis and the flow of the target protein through all stages of the B. subtilis secretion pathway is of crucial importance with respect to yield and quality of secreted heterologous proteins

Middle to Late Quaternary palaeolandscapes of the central Azraq Basin, Jordan: Deciphering discontinuous records of human-environment dynamics at the arid margin of the Levant

The Azraq oasis in the Eastern Desert of Jordan has produced considerable stone artefacts attributed to the early Palaeolithic, yet relatively few data are available regarding the chronology and palaeoenvironmental contexts of the remains. In this study, we present stratigraphic, sedimentological, and micropalaeontological analyses of the Late Acheulean site SM1 located within the former Shishan Marsh, which we combine with geochronological and sedimentological data from 13 neighbouring geological exposures to reconstruct landscape evolution at the western margin of the Shishan Marsh. We then discuss the Late Quaternary palaeolandscapes of the Greater Azraq Oasis Area over the past c. 350 ka. Our work demonstrates that the central Azraq Basin experienced three local wetting-drying cycles since the late Middle Pleistocene that would have dramatically shifted the quantity and distribution of freshwater resources, ranging from expansive wetland landscapes to desert refugia characterised by isolated spring pools—changes that would have significantly impacted the mobility decisions and settlement patterns of Palaeolithic inhabitants. Our study highlights that developing long-term records of human-environment dynamics in arid environments requires a mosaic approach to palaeoenvironmental reconstruction that is nested within a well-developed understanding of landscape evolution

Ultra-low background measurements of decayed aerosol filters RID B-6012-2011 RID G-8809-2011

Aerosol samples collected on filter media were analyzed using HPGe detectors employing varying background-reduction techniques in order to experimentally evaluate the opportunity to apply ultra-low background measurement methods to samples collected, for instance, by the Comprehensive Test Ban Treaty International Monitoring System (IMS). In this way, realistic estimates of the impact of low-background methodology on the sensitivity obtained in systems such as the IMS were assessed. The current detectability requirement of stations in the IMS is 30 mu Bq/m(3) of air for (140)Ba, which would imply similar to 10(6) fissions per daily sample. Importantly, this is for a fresh aerosol filter. One week of decay reduces the intrinsic background from radon daughters in the sample allowing much higher sensitivity measurement of relevant isotopes, including (131)I. An experiment was conducted in which decayed filter samples were measured at a variety of underground locations using Ultra-Low Background (ULB) gamma spectroscopy technology. The impacts of the decay and ULB are discussed