Feline hypersomatotropism and acromegaly tumorigenesis: a potential role for the AIP gene

Acromegaly in humans is usually sporadic, however up to 20% of familial isolated pituitary adenomas are caused by germline sequence variants of the aryl-hydrocarbon-receptor interacting protein (AIP) gene. Feline acromegaly has similarities to human acromegalic families with AIP mutations. The aim of this study was to sequence the feline AIP gene, identify sequence variants and compare the AIP gene sequence between feline acromegalic and control cats, and in acromegalic siblings. The feline AIP gene was amplified through PCR using whole blood genomic DNA from 10 acromegalic and 10 control cats, and 3 sibling pairs affected by acromegaly. PCR products were sequenced and compared with the published predicted feline AIP gene. A single nonsynonymous SNP was identified in exon 1 (AIP:c.9T > G) of two acromegalic cats and none of the control cats, as well as both members of one sibling pair. The region of this SNP is considered essential for the interaction of the AIP protein with its receptor. This sequence variant has not previously been reported in humans. Two additional synonymous sequence variants were identified (AIP:c.481C > T and AIP:c.826C > T). This is the first molecular study to investigate a potential genetic cause of feline acromegaly and identified a nonsynonymous AIP single nucleotide polymorphism in 20% of the acromegalic cat population evaluated, as well as in one of the sibling pairs evaluated

Lithium in the Symbiotic Mira V407 Cyg

We report an identification of the lithium resonance doublet LiI 6708A in the spectrum of V407 Cyg, a symbiotic Mira with a pulsation period of about 745 days. The resolution of the spectra used was R~18500 and the measured equivalent width of the line is ~0.34A. It is suggested that the lithium enrichment is due to hot bottom burning in the intermediate mass AGB variable, although other possible origins cannot be totally ruled out. In contrast to lithium-rich AGB stars in the Magellanic clouds, ZrO 5551A, 6474A absorption bands were not found in the spectrum of V407Cyg. These are the bands used to classify the S-type stars at low-resolution. Although we identified weak ZrO 5718A, 6412A these are not visible in the low-resolution spectra, and we therefore classify the Mira in V407 Cyg as an M type. This, together with other published work, suggests lithium enrichment can precede the third dredge up of s-process enriched material in galactic AGB stars.Comment: 4 pages, 2 figures, to be published in MNRA

The visibility of the Galactic bulge in optical surveys. Application to the Gaia mission

The bulge is a region of the Galaxy which is of tremendous interest for understanding Galaxy formation. However, measuring photometry and kinematics in it raises several inherent issues, like high extinction in the visible and severe crowding. Here we attempt to estimate the problem of the visibility of the bulge at optical wavelengths, where large CCD mosaics allow to easily cover wide regions from the ground, and where future astrometric missions are planned. Assuming the Besancon Galaxy model and high resolution extinction maps, we estimate the stellar density as a function of longitude, latitude and apparent magnitude and we deduce the possibility of reaching and measuring bulge stars. The method is applied to three Gaia instruments, the BBP and MBP photometers, and the RVS spectrograph. We conclude that, while in the BBP most of the bulge will be accessible, in the MBP there will be a small but significant number of regions where bulge stars will be detected and accurately measured in crowded fields. Assuming that the RVS spectra may be extracted in moderately crowded fields, the bulge will be accessible in most regions apart from the strongly absorbed inner plane regions, because of high extinction, and in low extinction windows like the Baades's window where the crowding is too severe.Comment: 11 pages, 9 figures, accepted for publication in A&A, latex using A&A macro

Fractal scan strategies for selective laser melting of ‘unweldable’ nickel superalloys

The high thermal gradients experienced during manufacture via selective laser melting commonly result in cracking of high γ/γ′ Nickel based superalloys. Such defects cannot be tolerated in applications where component integrity is of paramount importance. To overcome this, many industrial practitioners make use of hot isostatic pressing to ‘heal’ these defects. The possibility of such defects re-opening during the component life necessitates optimisation of SLM processing parameters in order to produce the highest bulk density and integrity in the as-built state. In this paper, novel fractal scanning strategies based upon mathematical fill curves, namely the Hilbert and Peano-Gosper curve, are explored in which the use of short vector length scans, in the order of 100 μm, is used as a method of reducing residual stresses. The effect on cracking observed in CM247LC superalloy samples was analysed using image processing, comparing the novel fractal scan strategies to more conventional ‘island’ scans. Scanning electron microscopy and energy dispersive X-ray spectroscopy was utilised to determine the cracking mechanisms. Results show that cracking occurs via two mechanisms, solidification and liquation, with a strong dependence on the laser scan vectors. Through the use of fractal scan strategies, bulk density can be increased by 2 ± 0.7% when compared to the ‘island’ scanning, demonstrating the potential of fractal scan strategies in the manufacture of typically ‘unweldable’ nickel superalloys

Syndromic surveillance to assess the potential public health impact of the Icelandic volcanic ash plume across the United Kingdom, April 2010

The Eyjafjallajökull volcano in Iceland erupted on 14 April 2010 emitting a volcanic ash plume that spread across the United Kingdom and mainland Europe. The Health Protection Agency and Health Protection Scotland used existing syndromic surveillance systems to monitor community health during the incident: there were no particularly unusual increases in any of the monitored conditions. This incident has again demonstrated the use of syndromic surveillance systems for monitoring community health in real time

The nuclear star cluster of the Milky Way

The nuclear star cluster of the Milky Way is a unique target in the Universe. Contrary to extragalactic nuclear star clusters, using current technology it can be resolved into tens of thousands of individual stars. This allows us to study in detail its spatial and velocity structure as well as the different stellar populations that make up the cluster. Moreover, the Milky Way is one of the very few cases where we have firm evidence for the co-existence of a nuclear star cluster with a central supermassive black hole, Sagittarius A*. The number density of stars in the Galactic center nuclear star cluster can be well described, at distances $\gtrsim1$ pc from Sagittarius A*, by a power-law of the form $\rho(r)\propto r^{-\gamma}$ with an index of $\gamma\approx1.8$. In the central parsec the index of the power-law becomes much flatter and decreases to $\gamma\approx1.2$. We present proper motions for more than 6000 stars within 1 pc in projection from the central black hole. The cluster appears isotropic at projected distances $\gtrsim0.5$ pc from Sagittarius A*. Outside of 0.5 pc and out to 1.0 pc the velocity dispersion appears to stay constant. A robust result of our Jeans modeling of the data is the required presence of $0.5-2.0\times10^{6} M_{\odot}$ of extended (stellar) mass in the central parsec of the Galaxy.Comment: To appear in the proceedings of "The Universe under the Microscope - Astrophysics at High Angular Resolution", Journal of Physics:Conference Series (IOP; http://www.iop.org/EJ/conf) This version has been slightly modified (e.g. double-log plot in right hand panel of Figure 5

Strategies for conducting situated studies of technology use in hospitals

Ethnographic methods are widely used for understanding situated practices with technology. When authors present their data gathering methods, they almost invariably focus on the bare essentials. These enable the reader to comprehend what was done, but leave the impression that setting up and conducting the study was straightforward. Text books present generic advice, but rarely focus on specific study contexts. In this paper, we focus on lessons learnt by non-clinical researchers studying technology use in hospitals: gaining access; developing good relations with clinicians and patients; being outsiders in healthcare settings; and managing the cultural divide between technology human factors and clinical practice. Drawing on case studies across various hospital settings, we present a repertoire of ways of working with people and technologies in these settings. These include engaging clinicians and patients effectively, taking an iterative approach to data gathering and being responsive to the demands and opportunities provided by the situation. The main contribution of this paper is to make visible many of the lessons we have learnt in conducting technology studies in healthcare, using these lessons to present strategies that other researchers can take up

Design Principles for Plasmonic Nanoparticle Devices

For all applications of plasmonics to technology it is required to tailor the resonance to the optical system in question. This chapter gives an understanding of the design considerations for nanoparticles needed to tune the resonance. First the basic concepts of plasmonics are reviewed with a focus on the physics of nanoparticles. An introduction to the finite element method is given with emphasis on the suitability of the method to nanoplasmonic device simulation. The effects of nanoparticle shape on the spectral position and lineshape of the plasmonic resonance are discussed including retardation and surface curvature effects. The most technologically important plasmonic materials are assessed for device applicability and the importance of substrates in light scattering is explained. Finally the application of plasmonic nanoparticles to photovoltaic devices is discussed.Comment: 29 pages, 15 figures, part of an edited book: "Linear and Non-Linear Nanoplasmonics

Meso-scale defect evaluation of selective laser melting using spatially resolved acoustic spectroscopy

Developments in additive manufacturing technology are serving to expand the potential applications. Critical developments are required in the supporting areas of measurement and in process inspection to achieve this. CM247LC is a nickel superalloy that is of interest for use in aerospace and civil power plants. However, it is difficult to process via selective laser melting (SLM) as it suffers from cracking during rapid cooling and solidification. This limits the viability of CM247LC parts created using SLM. To quantify part integrity, spatially resolved acoustic spectroscopy (SRAS) has been identified as a viable non-destructive evaluation technique. In this study, a combination of optical microscopy and SRAS was used to identify and classify the surface defects present in SLM-produced parts. By analysing the datasets and scan trajectories, it is possible to correlate morphological information with process parameters. Image processing was used to quantify porosity and cracking for bulk density measurement. Analysis of surface acoustic wave data showed that an error in manufacture in the form of an overscan occurred. Comparing areas affected by overscan with a bulk material, a change in defect density from 1.17% in the bulk material to 5.32% in the overscan regions was observed, highlighting the need to reduce overscan areas in manufacture