Alterations in microbial community composition with increasing fCO2: a mesocosm study in the eastern Baltic Sea

Ocean acidification resulting from the uptake of anthropogenic carbon dioxide (CO₂) by the ocean is considered a major threat to marine ecosystems. Here we examined the effects of ocean acidification on microbial community dynamics in the eastern Baltic Sea during the summer of 2012 when inorganic nitrogen and phosphorus were strongly depleted. Large-volume in situ mesocosms were employed to mimic present, future and far future CO₂ scenarios. All six groups of phytoplankton enumerated by flow cytometry ( &lt;  20 µm cell diameter) showed distinct trends in net growth and abundance with CO₂ enrichment. The picoeukaryotic phytoplankton groups Pico-I and Pico-II displayed enhanced abundances, whilst Pico-III, <i>Synechococcus</i> and the nanoeukaryotic phytoplankton groups were negatively affected by elevated fugacity of CO₂ (<i>f</i>CO₂). Specifically, the numerically dominant eukaryote, Pico-I, demonstrated increases in gross growth rate with increasing <i>f</i>CO₂ sufficient to double its abundance. The dynamics of the prokaryote community closely followed trends in total algal biomass despite differential effects of <i>f</i>CO₂ on algal groups. Similarly, viral abundances corresponded to prokaryotic host population dynamics. Viral lysis and grazing were both important in controlling microbial abundances. Overall our results point to a shift, with increasing <i>f</i>CO₂, towards a more regenerative system with production dominated by small picoeukaryotic phytoplankton.</p

Effect of CO2 enrichment on bacterial metabolism in an Arctic fjord

he anthropogenic increase of carbon dioxide (CO2) alters the seawater carbonate chemistry, with a decline of pH and an increase in the partial pressure of CO2 (pCO2). Although bacteria play a major role in carbon cycling, little is known about the impact of rising pCO2 on bacterial carbon metabolism, especially for natural bacterial communities. In this study, we investigated the effect of rising pCO2 on bacterial production (BP), bacterial respiration (BR) and bacterial carbon metabolism during a mesocosm experiment performed in Kongsfjorden (Svalbard) in 2010. Nine mesocosms with pCO2 levels ranging from ca. 180 to 1400 μatm were deployed in the fjord and monitored for 30 days. Generally BP gradually decreased in all mesocosms in an initial phase, showed a large (3.6-fold average) but temporary increase on day 10, and increased slightly after inorganic nutrient addition. Over the wide range of pCO2 investigated, the patterns in BP and growth rate of bulk and free-living communities were generally similar over time. However, BP of the bulk community significantly decreased with increasing pCO2 after nutrient addition (day 14). In addition, increasing pCO2 enhanced the leucine to thymidine (Leu : TdR) ratio at the end of experiment, suggesting that pCO2 may alter the growth balance of bacteria. Stepwise multiple regression analysis suggests that multiple factors, including pCO2, explained the changes of BP, growth rate and Leu : TdR ratio at the end of the experiment. In contrast to BP, no clear trend and effect of changes of pCO2 was observed for BR, bacterial carbon demand and bacterial growth efficiency. Overall, the results suggest that changes in pCO2 potentially influence bacterial production, growth rate and growth balance rather than the conversion of dissolved organic matter into CO2

Dynamics of nutrients, total organic carbon, prokaryotes and viruses in onboard incubations of cold-water corals

The potential influence of the cold-water corals (CWCs) Lophelia pertusa and Madrepora oculata on the dynamics of inorganic nutrient and total organic carbon (TOC) concentrations and the abundances of prokaryotes and viruses in bottom water was assessed in onboard incubation experiments. Ammonium, nitrite, dissolved inorganic nitrogen (DIN), dissolved inorganic phosphorus (DIP) and TOC concentrations and N:P ratios were typically higher in incubation water with corals than in controls, whereas nitrate concentrations did not reveal a clear trend. Mucus release (normalized to coral surface) was estimated by the net increase rate of TOC concentrations and averaged 23 +/- 6 mg C m(-2) h(-1) for L. pertusa and 21 +/- 8 mg C m(-2) h(-1) for M. oculata. Prokaryotic and viral abundance and turnover rates were typically stimulated in incubation water with corals. This estimated prokaryotic stimulation averaged 6.0 +/- 3.0x10(9) cells m(-2) h(-1) for L. pertusa and 8.4 +/- 2.9x10(9) cells m(-2) h(-1) for M. oculata, whereas the estimated viral stimulation averaged 15.6 +/- 12.7x10(9) particles m(-2) h(-1) for L. pertusa and 4.3 +/- 0.4x10(9) particles m(-2) h(-1) M. oculata. Our data suggest that prokaryotes and viruses are released from corals and that nutrient and mucus release enhanced prokaryotic and viral production. The result of this stimulation could be a fuelling of bottom water in CWC reefs with nutrients and organic matter and consequently an enhancement of microbe-mediated processes

The Impact of Isospin Breaking on the Distribution of Transition Probabilities

In the present paper we investigate the effect of symmetry breaking in the statistical distributions of reduced transition amplitudes and reduced transition probabilities. These quantities are easier to access experimentally than the components of the eigenvectors and were measured by Adams et al. for the electromagnetic transitions in ^{26}Al. We focus on isospin symmetry breaking described by a matrix model where both, the Hamiltonian and the electromagnetic operator, break the symmetry. The results show that for partial isospin conservation, the statistical distribution of the reduced transition probability can considerably deviate from the Porter-Thomas distribution.Comment: 16 pages, 8 figures, submitted to PR

Arctic microbial community dynamics influenced by elevated CO2 levels

The Arctic Ocean ecosystem is particularly vulnerable to ocean acidification (OA) related alterations due to the relatively high CO2 solubility and low carbonate saturation states of its cold surface waters. Thus far, however, there is only little known about the consequences of OA on the base of the food web. In a mesocosm CO2-enrichment experiment (overall CO2 levels ranged from ~ 180 to 1100 μatm) in Kongsfjorden off Svalbard, we studied the consequences of OA on a natural pelagic microbial community. OA distinctly affected the composition and growth of the Arctic phytoplankton community, i.e. the picoeukaryotic photoautotrophs and to a lesser extent the nanophytoplankton thrived. A shift towards the smallest phytoplankton as a result of OA will have direct consequences for the structure and functioning of the pelagic food web and thus for the biogeochemical cycles. Besides being grazed, the dominant pico- and nanophytoplankton groups were found prone to viral lysis, thereby shunting the carbon accumulation in living organisms into the dissolved pools of organic carbon and subsequently affecting the efficiency of the biological pump in these Arctic waters