462 research outputs found

    LABOR MARKET IN CHINA: LABOR RIGHTS, INTERNAL MIGRATION, AND CURRENT TRENDS

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    Uno de los pilares del crecimiento económico de China es su mercado laboral. Detrás de la segunda economía más grande del mundo, compuesta por una mano de obra de más de 900 millones y basada en una sólida y creciente estabilidad económica, se encuentra una población activa sujeta a condiciones laborales muy diferentes a las de sus competidores occidentales, creando así un ventaja y un alto grado de atractivo para los inversores extranjeros. Este documento explora el mercado laboral y los derechos laborales en China, el papel desempeñado por el gobierno, las organizaciones de trabajadores y los actores internacionales, los flujos migratorios y las perspectivas de un país considerado como la "fábrica del mundo"

    Evaluation of different API systems for identification of porcine Pasteurella multocida isolates

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    4 p.An exhaustive biochemical characterisation of 60 porcine Pasteurella multocida clinical isolates recovered from lesions indicative of pneumonia, previously confirmed by PCR and all belonging to the capsular serogroup A, was performed by means of four commercial systems. The API 20NE correctly identified almost all isolates (95%), but only 60% could be ascribed to this species by the API 20E method. The high diversity exhibited by the API 50CHB/E system, with six different patterns, does not advise its use as additional system for a definitive identification at the species level, but this method could be a potential tool for characterising P. multocida isolates below this level. The more uniform reactions yielded by the API ZYM test make this system helpful in the confirmatory identification of this organism. The high variability (20 profiles) obtained when the four systems are taken together also suggests their usefulness for epidemiological purposes in order to sub-type P. multocida isolatesS

    Value of Indirect Hemagglutination and Coagglutination Tests for Serotyping Haemophilus parasuis

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    P. 880-882An indirect hemagglutination test (IHA) and a coagglutination test (CA) were evaluated using saline, boiled, and autoclaved extracts for serotyping Haemophilus parasuis. CA showed several cross-reactions, whereas IHA gave rise to specific reactions, with minor exceptions. IHA was further compared with the immunodiffusion test (the “gold standard”) for the serotyping of 67 field isolates. As a conclusion, IHA is recommended as a useful method for sensitive and specific serotyping of H. parasuisS

    Molecular characterization of Haemophilus parasuis ferric hydroxamate uptake (fhu) genes and constitutive expression of the FhuA receptor

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    P. 49-59Bacteria have evolved a set of highly specialized proteins to capture iron in irondepleted environments. The acquisition and uptake of iron present in the extracellular milieu of eukaryotic organisms is indispensable for the growth and survival of microbial pathogens in the course of infection. Haemophilus parasuis is the causative agent of Glässer disease, which is responsible for considerable financial losses in pig-rearing worldwide. To gain insight into the mechanisms involved in siderophore-mediated iron uptake in H. parasuis, genes in the H. parasuis ferric hydroxamate uptake (Fhu) region were amplified in the work being reported here. As has been described in A. pleuropneumoniae, an Fhu genomic region was also present in H. parasuis, being composed of four potential consecutive open reading frames (ORF) designated as fhuC, fhuD, fhuB, and fhuA, respectively. By immunoblotting, using a cross-reactive polyclonal antibody raised against Actinobacillus pleuropneumoniae FhuA protein, it was demonstrated that this protein was constitutively expressed in H. parasuis and its level of expression was not modified under conditions of restricted iron availability. This is the first report describing the presence of the fhu genes in H. parasuis. Our results indicate that FhuA protein expression is not affected under ironrestricted conditions, however, it is one of the targets of the humoral immune responseS

    Diseño y análisis de un sistema de instrumentación y automatización industrial aplicado al proceso de pasteurización de una planta de elaboración de cerveza

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    In the pasteurization process, the microorganisms that are used for the fermentation and rotting of bier are mainly eliminated by high temperatures. This project is about a study of feasibility for the implementation, cost analysis and the rent of the automation for the process of equipment for flash pasteurization in a bier industry. The main point of this job is to obtain the advantages in costs, production and mainly in the quality of the final product of the pasteurization working with an automation process. For this, we have considered a Programmable Logic Controller for the acquisition, treatment and process of the signals generated by the field instruments. The process will be monitored and supervised continuously, so and SCADA system will be developed for this task in an operation room, and for the field will be implemented a Human Machine Interface. It will be considered the different rules and standards that are in force in our country at the moment of the design

    Haemophilus parasuis serovar 5 Nagasaki strain adheres and invades PK-15 cells

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    7 p.Haemophilus parasuis is the agent responsible for causing Gla¨ sser’s disease, which is characterized by fibrinous polyserositis, polyarthritis and meningitis in pigs. The purpose of this study was to investigate the in vitro ability of two H. parasuis serovars of different virulence (serovar 5, Nagasaki strain, highly virulent, belonging to serovar 5, and SW114 strain, nonvirulent, belonging to serovar 3) to adhere to and invade porcine kidney epithelial cells (PK-15 line). Nagasaki strain was able to attach at high levels from 60 to 180 min of incubation irrespective of the concentrations compared (107–1010 CFU), and a substantial increase of surface projections could be seen in PK-15 cells by scanning electron microscopy. This virulent strain was also able to invade effectively these epithelial cells, and the highest invasion capacity was reached at 180 min of infection. On the contrary, nonvirulent SW114 strain hardly adhered to PK-15 cells, and it did not invade these cells, thus suggesting that adherence and invasion of porcine kidney epithelial cells could be a virulence mechanism involved in the lesions caused by H. parasuis Nagasaki strain in this organS
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