Cutaneous graft-versus-host disease after hematopoietic stem cell transplant - a review

Graft-versus-host disease (GVHD) is a major complication of allogeneic hematopoietic stem cell transplants (allo-HSCT) associated with significant morbidity and mortality. The earliest and most common manifestation is cutaneous graft-versus-host disease. This review focuses on the pathophysiology, clinical features, prevention and treatment of cutaneous graft-versus-host disease. We discuss various insights into the disease’s mechanisms and the different treatments for acute and chronic skin graft-versus-host disease

Validez y confiabilidad de la Escala Satisfacción Familiar en estudiantes universitarios de Lima Metropolitana, Perú

La presente investigación tuvo como objetivo analizar las propiedades psicométricas de la Escala de Satisfacción Familiar (FSS) en una muestra de 607 estudiantes de una universidad privada en Lima Metropolitana, con edades que oscilan entre 16 a 28 años. Se tradujo la escala con el método de traducción inversa; se realizaron análisis factorial exploratorio y confirmatorio, identificando valores óptimos para una solución unifactorial. Asimismo, se analizó la relación con la variable comunicación familiar, que señala una correlación positiva, directa y con un tamaño del efecto moderado. En el análisis de invarianza según sexo, se encontró un nivel de invarianza débil. Finalmente, se reportan valores óptimos de confiabilidad. Se concluye que la FSS presenta evidencias de validez y confiabilidad, recomendando su uso

Application of stable isotope analysis to differentiate shrimp extracted by industrial fishing or produced through aquaculture practices.

Carbon and nitrogen stable isotope values were determined in Pacific white shrimp (Litopenaeus vannamei) with the objective of discriminating animals produced through aquaculture practices from those extracted from the wild. Farmed animals were collected at semi-intensive shrimp farms in Mexico and Ecuador. Fisheries-derived shrimps were caught in different fishing areas representing two estuarine systems and four open sea locations in Mexico and Ecuador. Carbon and nitrogen stable isotope values (13CVPDB and 15NAIR) allowed clear differentiation of wild from farmed animals. 13CVPDB and 15NAIR values in shrimps collected in the open sea were isotopically enriched (−16.99‰ and 11.57‰), indicating that these organisms belong to higher trophic levels than farmed animals. 13CVPDB and 15NAIR values of farmed animals (−19.72‰ and 7.85‰, respectively) partially overlapped with values measured in animals collected in estuaries (−18.46‰ and 5.38‰, respectively). Canonical discriminant analysis showed that when used separately and in conjunction, 13CVPDB and I5NAIR values were powerful discriminatory variables and demonstrate the viability of isotopic evaluations to distinguish wild-caught shrimps from aquaculture shrimps. Methodological improvements will define a verification tool to support shrimp traceability protocols

Sensor Networks and derived products at Biscay AGL observatory. State of the art operational oceanography at IEO

Since 1991, shelf and slope waters of the Southern Bay of Biscay are regularly sampled in a monthly hydrographical section north of Santander, and on June 2007, an ocean meteorological buoy was moored at the end of Santander Section (www. boya_agl.st.ieo.es). Both are part of IEOOS (IEO Observing System). Biscay AGL is one observatory for the EU FixO3 project. Many sensor networks have been deployed to monitor marine environment, and more will follow in the future. Due to the large number of sensor technologies, integrating diverse sensors into observation systems is not straightforward. By defining standardized service interfaces (like those based on OGC standards) it is possible to enable access to sensor networks and archived sensor data that can be discovered and accessed using standard protocols and application programming interfaces, therefore complying with the requirements of the INSPIRE directive. Future developments include the deployment of a full sensor network as well as adding new devices to the Biscay AGL tool in order to achieve a deeper knowledge of the ocean. Biscay AGL is more than the combination of the AGL Buoy and the hydrographical samplings. This observatory produces not only time series of several parameters at different time resolutions but also derived products, both in real and in delayed time. Derived products from this buoy include annual cycles as well as anomalies of physical and biogeochemical magnitudes like air-sea heat fluxes, salinity and water temperatures, sub inertial currents, surface chlorophyll. Different products are derived from in-situ measurements at the AGL buoy like estimates of the mixed layer depth, wind and currents roses and wave intensity diagrams

Research and operational products from the combination of a monthly hydrographic station and an oceanic buoy: The Biscay AGL fixed-point water column observatory.

Long term time series are an important tool for increasing the knowledge of ocean processes as well as for studying water masses variability in different time scales and changes and tendencies in marine ecosystems. Time series has been classically obtained by oceanographic ships that regularly cover standard sections and stations. From 1991, shelf and slope waters of the Southern Bay of Biscay are regularly sampled in a monthly hydrographic line north of Santander to a depth of 1000 m in early stages and for the whole water column down to 2580 m in recent times. Nearby, in June 2007, the IEO deployed an oceanic-meteorological buoy (AGL Buoy, 43º 50.67’N; 3º 46.20’W, and 40 km offshore, www.boya-agl.st.ieo.es). The long-term hydrographical record have allowed to define the seasonality, trends, and interannual variability at all levels, including the mixing layer and the main water masses North Atlantic Central Water and Mediterranean Water. The relation of these changes with high frequency surface conditions has been examined using the AGL buoy data from 2007 as well as satellite and reanalysis data. On that context and using that combination of sources, some products and quality controlled series of high interest and utility for scientific purposes have been developed and are offered hourly in the web page. Main products obtained are: SST and SSS anomalies, wave significant height character with respect to monthly average, and currents with respect to seasonal averages. Ocean-atmosphere heat fluxes (latent and sensible) are computed from the buoy atmospheric and oceanic measurements. Estimations of the mixed layer depth and bulk series at different water levels are provided in a monthly basis. Quality controlled series are provided for sea surface salinity, oxygen and chlorophyll data. Some sensors are particularly affected by biofouling, and monthly visits to the buoy permit to follow these sensors behaviour. Chlorophyll-fluorescence sensor is the main concern, but Dissolved Oxygen sensor is also problematic. Periods of realistic smooth variations present strong offset that is corrected based on the Winkler analysis of water samples. The incorporation of these observatories on larger scale research programs, as done in 2003 in the framework of the VACLAN and COVACLAN projects, is important in order to provide them with a larger spatial dimension and maximize its utility for process-oriented studies. In 2003, the Santander section was extended 90 miles offshore in the framework of a large-scale hydrographic and circulation monitoring program. Partnerships in a large EU project as FixO3 has provided tools for coordination, homogenization and data validation as well as improve the use of chemical-biological data.0,000

Evaluation of the Expression of Genes Associated with Inflammation and Apoptosis in Androgenetic Alopecia by Targeted RNA-Seq

Abstract Androgenetic alopecia (AGA) or male pattern baldness is the most common form of hair loss in humans. Despite being a very frequent dermatological entity, molecular pathophysiology remains unclear. Several authors relate the presentation of AGA with a premature apoptotic process during the anagen phase and with an inflammatory microenvironment in the hair follicle. We evaluated a panel of 30 genes associated with inflammation and apoptosis in 5 AGA patients by targeted RNA-Seq. WNT7A gene was highly expressed in patients in stages 3V to 5 on the Hamilton-Norwood scale compared to patients with 5A stage. CASP7 and TNF genes were overexpressed in stages 3V and 4 compared to stages 5 and 5A. Overexpression of these genes detected only at early stages of AGA proves the role of WNT pathway, apoptosis, and inflammation in the development of this disorder

Exploring the contribution of dietary protein from poultry by-product meal and fish meal to the growth of catfish Ictalurus punctatus by means of nitrogen stable isotopes

The natural nitrogen stable isotope signatures (δ 15N) found in poultry by-product meal (PBM) and fish meal (FM) were used to estimate the relative contribution of dietary nitrogen supplied by both ingredients to the somatic growth of juvenile channel catfish Ictalurus punctatus. Six isonitrogenous and isoenergetic experimental diets were formulated using FM and PBM. Two of these diets consisted of isotopic controls having only one ingredient supplying dietary nitrogen, either FM or PBM. Four combined diets were formulated with varying proportions of these ingredients in order to supply high proportions of PBM (FM:PBM, 50:50, 35:65 20:80 and 5:95) on a nitrogen basis. There were significant differences in mean final weight of fish at the end of the trial. Lower growth was observed as the dietary level of PBM increased. In order to determine the relative contributions of the dietary nitrogen supplied by FM and PBM to catfish growth, an isotopic mixing model was applied. Results indicated that the incorporation of dietary nitrogen supplied by PBM was equivalent to the die tary proportions. The dietary nitrogen available in combined diets containing 50, 65 and 80% of PBM was incorporated in fish bodies as 50, 62 and 81%, respectively. However, high incorporation of dietary nitrogen from PBM was not always reflected in higher growth rates. Results demonstrate the viable use of stable isotopes to determine the allocation of dietary nitrogen and indicate that practical diets for catfish can be formulated with levels of PBM as high as 65% without affecting growth and survival

New developments on Biscay-AGL Observatory. From derived products to sensor networks.

Biscay-AGL Observatory. Since 1991, shelf and slope waters of the Southern Bay of Biscay are regularly sampled in a monthly hydrographical section north of Santander to a maximum depth of 2500m, as part of the IEO Radiales program. On June 2007, an ocean-meteorological buoy (AGL) was moored at the end of Santander standard section, 22nm north at 2850m depth, to complete the ocean information with the ocean-atmosphere interaction. All of them are part of IEO Observing System (1). The integrated system of AGL and its nearest hydrographic station (2600m depth) is named Biscay-AGL observatory. It is also one station for the EU FixO3 project. Joint resources and systematic analysis lead to a powerful tool, which is much more than the combination of the AGL buoy and the hydrographical samplings. Data Access. All AGL buoy collected data are added to the local database sited at IEO-Santander in real-time and, after rutinary automatised quality controls, they are immediately available through its dedicated webpage (www.boya_agl.st.ieo.es). Monthly CTDO2 data from the hydrographic section are lab-calibrated in order to obtain acurated values of salinity, dissolved oxygen and density, and added to the long-term time series. Biscay-AGL data are quality controlled, flagged and formatted according internationally agreed standards (2, 3) and routinely sent to the IEO Datacenter. This added-value controlled data are incorporated to the IEO permanent archive and made freely available through the SeaDataNet infrastructure for data access and discovery. Derived products. Data acquired by Biscay-AGL may be displayed as timeseries as usual, but end-users are benefited by derived products which provide direct information. A recently developed software tool produces not only timeseries of several parameters at different time resolutions but also derived products, both real and delayed time. Derived products from this buoy include, but not only, annual cycles as well as anomalous values, air-sea heat fluxes, salinity and water temperature anomalies, subinertial currents series, chlorophyll surface series, estimations of the mixed layer depth and wind and currents roses. Sensor Web Enablement (SWE). Sensor Web infrastructures are setup to access real-time data observed by sensors. This system has been implemented in AGL buoy sensors in order to simplify the retrieved events and alerts triggered through sensors. All those functionalities of the Sensor Web are provided in an interoperable way, following the standards stablished by the Open Geospatial Consortium (OGC). By defining standardized service interfaces, these services hides the heterogeneity of the sensor network, its communication details, enabling the access to archived sensor data that can be discovered and accessed using standard protocols and application programming interfaces

The Spanish Institute of Oceanography Observing System around N and NW Iberia

In the late 80's, the Spanish Institute of Oceanography (IEO) began some ambitious programs of monitoring around the Iberian Peninsula. The coastal observing system core structure was the Radiales program, a series of transects nearby most coastal IEO laboratories where a minimum set of hydrographical, chemical and biological parameters is sampled monthly. The sections are designed to be completed in one-day journeys so the system is supported by quite small vessels, mainly covering the continental shelf with the noteworthy exception of the Santander Section, where the proximity of the shelf-break allows sampling deep waters monthly. During the last 20 years the program has supported many specific scientific research projects and has created a detailed description of seasonal to interannual variability in the coastal ecosystem that serves as a baseline to explore long-term changes or trends...Versión de auto

Las enferemedades emergentes transmitidas por vectores: Paludismo, dengue, chinkunguya y zika.

Los vectores son animales que transmiten patógenos, entre ellos parásitos, de una persona (o animal) infectada a otra y ocasionan enfermedades graves en el ser humano. Estas enfermedades emergentes y re-emergentes representan un grave problema de salud pública en regiones tropicales y subtropicales, donde gran parte de la población está en riesgo. Los países de escasos recursos son, particularmente, vulnerables al impacto social y económico de estas enfermedades, debido a los recursos limitados en el sector de salud pública para el manejo y prevención de estos padecimiento