54 research outputs found
The Iowa Homemaker vol.20, no.2
Your College Home, Editor, page 1
Home Economics Cabinet, Florence Byrnes, page 2
Study on a Budget, Dorothy Lee Conquest, page 4
Forward March with Confidence, Eleanor White, page 6
Home Economist from Far Away, Ruby Jackson, page 7
It’s a Man’s World, Gaynold Carroll, page 8
Art, Music, and Literature Mingle, Nancy Mason, page 10
Who’s Who on Campus, Dorothy Anne Roost, page 11
What’s New in Home Economics, page 12
Women’s Athletics Will Enter New Home, Jeanette Foster, page 14
Personalize Your Letter Writing, Virginia Kirkpatrick, page 15
Behind Bright Jackets, Marjorie Thomas, page 16
Coed Concoctions, Marian Dougan, page 18
Alums in the News, Bette Simpson, page 20
Give Beauty a Chance, Ida Halpin, page 22
Journalistic Spindles, Ruth Jensen, page 2
The Iowa Homemaker vol.21, no.5
Candles Spread Christmas Cheer, Mary Ellen Sullivan, page 2
Milk Strengthens Defense, Betty Ann Iverson, page 3
American Wares Set Nation’s Tables, Ann Koebel, page 4
New World Harbours Refugees, Dorothy Ann Klein, page 5
Sally Anticipates a Gala Season, Mary Lou Springer, page 6
Applied Art Highlights, Lila Williamson, page 8
Bookmarks, Julie Wendel, page 9
What’s New in Home Economics, Dorothy Olson, page 10
For Holiday Shoppers, Betty Roth, page 12
Residence Halls Honor Women Leaders, Dorothy Gross, page 13
Express Ingenuity in Greetings, Doris Plagge, page 14
Alums in the News, Bette Simpson, page 15
Fruit Cakes for Holidays, Margaret Anne Clark, page 16
Across Alumnae Desks, Marjorie Thomas, page 18
Journalistic Spindles, Florence Byrnes, page 2
Introducing - The Home Economics Cabinet
In short biographical sketches the heads of departments are presented by Florence Byrnes</p
Impact of delay to cryopreservation on RNA integrity and genome-wide expression profiles in resected tumor samples.
The quality of tissue samples and extracted mRNA is a major source of variability in tumor transcriptome analysis using genome-wide expression microarrays. During and immediately after surgical tumor resection, tissues are exposed to metabolic, biochemical and physical stresses characterized as "warm ischemia". Current practice advocates cryopreservation of biosamples within 30 minutes of resection, but this recommendation has not been systematically validated by measurements of mRNA decay over time. Using Illumina HumanHT-12 v3 Expression BeadChips, providing a genome-wide coverage of over 24,000 genes, we have analyzed gene expression variation in samples of 3 hepatocellular carcinomas (HCC) and 3 lung carcinomas (LC) cryopreserved at times up to 2 hours after resection. RNA Integrity Numbers (RIN) revealed no significant deterioration of mRNA up to 2 hours after resection. Genome-wide transcriptome analysis detected non-significant gene expression variations of -3.5%/hr (95% CI: -7.0%/hr to 0.1%/hr; p = 0.054). In LC, no consistent gene expression pattern was detected in relation with warm ischemia. In HCC, a signature of 6 up-regulated genes (CYP2E1, IGLL1, CABYR, CLDN2, NQO1, SCL13A5) and 6 down-regulated genes (MT1G, MT1H, MT1E, MT1F, HABP2, SPINK1) was identified (FDR <0.05). Overall, our observations support current recommendation of time to cryopreservation of up to 30 minutes and emphasize the need for identifying tissue-specific genes deregulated following resection to avoid misinterpreting expression changes induced by warm ischemia as pathologically significant changes
The Iowa Homemaker vol.20, no.2
Your College Home, Editor, page 1
Home Economics Cabinet, Florence Byrnes, page 2
Study on a Budget, Dorothy Lee Conquest, page 4
Forward March with Confidence, Eleanor White, page 6
Home Economist from Far Away, Ruby Jackson, page 7
It’s a Man’s World, Gaynold Carroll, page 8
Art, Music, and Literature Mingle, Nancy Mason, page 10
Who’s Who on Campus, Dorothy Anne Roost, page 11
What’s New in Home Economics, page 12
Women’s Athletics Will Enter New Home, Jeanette Foster, page 14
Personalize Your Letter Writing, Virginia Kirkpatrick, page 15
Behind Bright Jackets, Marjorie Thomas, page 16
Coed Concoctions, Marian Dougan, page 18
Alums in the News, Bette Simpson, page 20
Give Beauty a Chance, Ida Halpin, page 22
Journalistic Spindles, Ruth Jensen, page 24</p
Investigation of DNA repair-related SNPs underlying susceptibility to papillary thyroid carcinoma reveals MGMT as a novel candidate gene in Belarusian children exposed to radiation
BACKGROUND: Genetic factors may influence an individual's
sensitivity to ionising radiation and therefore modify his/her
risk of developing papillary thyroid carcinoma (PTC).
Previously, we reported that common single nucleotide
polymorphisms (SNPs) within the DNA damage recognition gene ATM
contribute to PTC risk in Belarusian children exposed to fallout
from the Chernobyl power plant accident. Here we explored in the
same population the contribution of a panel of DNA
repair-related SNPs in genes acting downstream of ATM. METHODS:
The association of 141 SNPs located in 43 DNA repair genes was
examined in 75 PTC cases and 254 controls from the Gomel region
in Belarus. All subjects were younger than 15 years at the time
of the Chernobyl accident. Conditional logistic regressions
accounting for radiation dose were performed with PLINK using
the additive allelic inheritance model, and a linkage
disequilibrium (LD)-based Bonferroni correction was used for
correction for multiple testing. RESULTS: The intronic SNP
rs2296675 in MGMT was associated with an increased PTC risk [per
minor allele odds ratio (OR) 2.54 95% CI 1.50, 4.30, P per
allele = 0.0006, P corr.= 0.05], and gene-wide association
testing highlighted a possible role for ERCC5 (P Gene = 0.01)
and PCNA (P Gene = 0.05) in addition to MGMT (P Gene = 0.008).
CONCLUSIONS: These findings indicate that several genes acting
in distinct DNA repair mechanisms contribute to PTC risk.
Further investigation is needed to decipher the functional
properties of the methyltransferase encoded by MGMT and to
understand how alteration of such functions may lead to the
development of the most common type of thyroid cancer
The Iowa Homemaker vol.21, no.5
Candles Spread Christmas Cheer, Mary Ellen Sullivan, page 2
Milk Strengthens Defense, Betty Ann Iverson, page 3
American Wares Set Nation’s Tables, Ann Koebel, page 4
New World Harbours Refugees, Dorothy Ann Klein, page 5
Sally Anticipates a Gala Season, Mary Lou Springer, page 6
Applied Art Highlights, Lila Williamson, page 8
Bookmarks, Julie Wendel, page 9
What’s New in Home Economics, Dorothy Olson, page 10
For Holiday Shoppers, Betty Roth, page 12
Residence Halls Honor Women Leaders, Dorothy Gross, page 13
Express Ingenuity in Greetings, Doris Plagge, page 14
Alums in the News, Bette Simpson, page 15
Fruit Cakes for Holidays, Margaret Anne Clark, page 16
Across Alumnae Desks, Marjorie Thomas, page 18
Journalistic Spindles, Florence Byrnes, page 20</p
Visualizing alternative futures
Results of the single marker association tests for each of the 141 DNA repair-related SNPs with MAF ≥ 0.05 present on the SNP Cancer Panel array which have passed genotyping quality controls. (XLS 145 kb
Samples description and microarray quality.
<p>Type of tumor and delay to tumor freezing are shown. RNA integrity is evaluated through the RIN number. The ratio of centiles P95/P05 reflects the overall strength of the signal compared to the background. The Pearson correlation coefficient (r<sup>2</sup>) shows the correlation between log-expression levels of the central and peripheral samples, for each tumor and each time to cryopreservation.</p><p>HCC: HepatoCellular Carcinoma.</p><p>LC: Lung Carcinoma.</p><p>ND: Not Determined.</p
- …