The Image of Destination Austria as perceived by British Tourists

Over the past decades, tourism destination image (TDI) has been one of the most widely studied areas of tourism research. However, only few authors have researched the image of destination Austria thus far. This study applies the concept of TDI to the context of Austria as tourist destination region (Leiper, 1995) and the country’s perceived image by British consumers. Apart from measuring cognitive and affective images, behavioural intentions of British consumers will be tested. The study attempts to identify potential marketing gaps, leading to the relatively low British visitor numbers over the last years (Office for National Statistics, 2015). Basing on the empirical findings of this study, marketing recommendations will be given, based on the 4P marketing mix approach (Jobber & Chadwick, 2013). This paper takes a mixed method approach to the measurement of the perceived Austrian TDI. This is in line with suggestions by Echtner & Ritchie (1993) and responds to a common criticism that purely quantitative methods cannot grasp the entirety of TDI (e.g. Pikkemaat, 2004), as TDI formation does not only include tangible, but also intangible/psychological factors, for which a purely structured measurement is unlikely to account (Chen & Tsai, 2007) The study finds that British consumers have a rather positive image of Austria. Nevertheless distinct weaknesses of the image could be identified which are likely to be caused by marketing gaps (Gursoy, 2011). The Recommendations of this study provide actionable suggestions for improving the marketing strategy in order to attract more British consumers to Austria, as the data in this study revealed evident potential within the British market

Isolation of highly selective IgNAR variable single-domains against a human therapeutic Fc scaffold and their application as tailor-made bioprocessing reagents

Funding This work was supported by the Industrial Biotechnology Innovation Centre, and Merck KGaA. Acknowledgements The authors would like to thank Iris Willenbücher and Kerstin Hallstein for the BIAcore™ analysis and Nadine Barron for the bio-layer interferometry work.Peer reviewedPostprin

Die Hofstelle naturnah und attraktiv gestalten

Der Leitfaden bietet zahlreiche Anregungen und praktische Tipps für Landwirte zur naturnahen Gestaltung des Hofgeländes. Der Inhalt reicht von der Pflanzung von Gehölzen, der Begrünung von Fassaden und Dächern, Ideen für den Garten über die Gestaltung von Zäunen und Mauern oder der Nutzung von Wasser bis hin zur gezielten Förderung von Insekten, Vögeln und Fledermäusen

USE OF THE FLIGHT TEST FACILITY MOCK-UPS IN THE EUROPEAN CLEAN SKY 2 PROGRAM

The Clean Sky 2 program unites all major European players in the aeronautical sector to conduct research on future, more energy efficient aircrafts with lower environmental footprint. Besides technologies developments, the demonstration of technologies in dedicated test environments is a key element of this research program. The Fraunhofer Flight Test Facility is such a platform for technology demonstration. Located in Holzkirchen, south of Munich, it hosts several aircraft fuselage structures and allows for indoor environmental tests under realistic temperature, moisture and pressure conditions. In this paper, demonstrations performed within Clean Sky 2 are highlighted. These include an adaptive ECS system, environmentally friendly fire protection, model validation tests for ETOPS considerations and cleaning and disinfection demonstrations

Einfluss von Thiophanat-Methyl und Methyl-Benzimidazol-2yl-Carbamat auf mykotoxinbildende Fusarium spp.

ZusammenfassungBei der Untersuchung des Einflusses von Thiophanat-Methyl und Methyl-Benzimidazol-2yl-Carbamat auf mykotoxinbildende Arten von Fusarium spp. konnte beobachtet werden, dass Thiophanat-Methyl einen deutlich stärkeren Einfluss auf die Biosynthese der Mykotoxine als auf das Wachstum der Pilze aufwies und die Respiration von Fusarium spp. im Flüssigmedium unabhängig vom Myzelwachstum gehemmt wurde. Die Ergebnisse weisen somit auf einen zusätzlichen Wirkungsmechanismus von Thiophanat-Methyl in Fusarium spp. hin. AbstractInvestigations into the effect of thiophanat-methyl and methyl-benzimidazole-2yl-carbamat on mycotoxinproducing species of Fusarium spp. showed that the influence on the biosynthesis of mycotoxins was more distinct than on the growth of the fungi. Moreover Fusarium spp. showed a reduced respiration at certain levels of TM that was not associated with an inhibited growth. The data indicate that there may be a mechanism of thiophanat-methyl by which the mycotoxinformation of Fusarium spp. could be inhibited independant from the growth of the mycelium.Keywords: Fusarium spp., thiophanat-methyl, carbendazim, mycotoxinproductio

A novel anti-B7-H3 chimeric antigen receptor from a single-chain antibody library for immunotherapy of solid cancers

B7-H3 (CD276) has emerged as a target for cancer immunotherapy by virtue of consistent expression in many malignancies, relative absence from healthy tissues, and an emerging role as a driver of tumor immune inhibition. Recent studies have reported B7-H3 to be a suitable target for chimeric antigen receptor-modified T cell (CAR-T) therapy using CARs constructed from established anti-B7-H3 antibodies converted into single-chain Fv format (scFv). We constructed and screened binders in an scFv library to generate a new anti-B7-H3 CAR-T with favorable properties. This allowed access to numerous specificities ready formatted for CAR evaluation. Selected anti-human B7-H3 scFvs were readily cloned into CAR-T and evaluated for anti-tumor reactivity in cytotoxicity, cytokine, and proliferation assays. Two binders with divergent complementarity determining regions were found to show optimal antigen-specific cytotoxicity and cytokine secretion. One binder in second-generation CD28-CD3ζ CAR format induced sustained in vitro proliferation on repeat antigen challenge. The lead candidate CAR-T also demonstrated in vivo activity in a resistant neuroblastoma model. An empirical approach to B7-H3 CAR-T discovery through screening of novel scFv sequences in CAR-T format has led to the identification of a new construct with sustained proliferative capacity warranting further evaluation

Uncovering tomato quantitative trait loci and candidate genes for fruit cuticular lipid composition using the Solanum pennellii introgression line population

[EN] The cuticle is a specialized cell wall layer that covers the outermost surface of the epidermal cells and has important implications for fruit permeability and pathogen susceptibility. In order to decipher the genetic control of tomato fruit cuticle composition, an introgression line (IL) population derived from a biparental cross between Solanum pennellii (LA0716) and the Solanum lycopersicum cultivar M82 was used to build a first map of associated quantitative trait loci (QTLs). A total of 24 cuticular waxes and 26 cutin monomers were determined. They showed changes associated with 18 genomic regions distributed in nine chromosomes affecting 19 ILs. Out of the five main fruit cuticular components described for the wild species S. pennellii, three of them were associated with IL3.4, IL12.1, and IL7.4.1, causing an increase in n-alkanes (>= C-30), a decrease in amyrin content, and a decrease in cuticle thickness of similar to 50%, respectively. Moreover, we also found a QTL associated with increased levels of amyrins in IL3.4. In addition, we propose some candidate genes on the basis of their differential gene expression and single nucleotide polymorphism variability between the introgressed and the recurrent alleles, which will be the subjects of further investigation.Research at the IBMCP was supported by the Spanish Ministry of Education and Culture (BIO2013-42193-R) and H2020 TRADITOM (634561). AA, AG, and J-PF-M thank COST FA1106 Quality Fruit for STSM and networking activities. This work was supported by the Israel Science Foundation (ISF) personal grant to AA (grant no. 646/11). We would like to thank the Adelis Foundation, the Leona M. and Harry B. Helmsley Charitable Trust, the Jeanne and Joseph Nissim Foundation for Life Sciences, Tom and Sondra Rykoff Family Foundation Research, and the Raymond Burton Plant Genome Research Fund for supporting AA's laboratory activity. AA is the incumbent of the Peter J. Cohn Professorial Chair. We are very grateful to Prof. Dani Zamir for providing us the S. pennellii IL collection and to Prof. Antonio Heredia for his valuable advice in preparing the manuscript for publication. We would like to acknowledge the help offered by the Electron Microscopy Unit at the WIS (Israel) for the TEM sample preparation and imaging, especially Elena Kartvelishvily, Eugenia Klein, and Eyal Shimoni. Finally, we would also like to thank Calanit Raanan and Tamara Berkutzki (Department of Veterinary Resources, WIS) for their help in tissue fixation and embedding, as well as Hanna Levanony (Department of Plant Sciences, WIS) for her help in tissue staining for the light microscopy studies.Fernández Moreno, JP.; Levy-Samoha, D.; Malitsky, S.; Monforte Gilabert, AJ.; Orzáez Calatayud, DV.; Aharoni, A.; Granell Richart, A. (2017). Uncovering tomato quantitative trait loci and candidate genes for fruit cuticular lipid composition using the Solanum pennellii introgression line population. Journal of Experimental Botany. 68(11):2703-2716. https://doi.org/10.1093/jxb/erx134S27032716681