Hydraulic Transport Across Hydrophilic and Hydrophobic Nanopores: Flow Experiments with Water and n-Hexane

We experimentally explore pressure-driven flow of water and n-hexane across nanoporous silica (Vycor glass monoliths with 7 or 10 nm pore diameters, respectively) as a function of temperature and surface functionalization (native and silanized glass surfaces). Hydraulic flow rates are measured by applying hydrostatic pressures via inert gases (argon and helium, pressurized up to 70 bar) on the upstream side in a capacitor-based membrane permeability setup. For the native, hydrophilic silica walls, the measured hydraulic permeabilities can be quantitatively accounted for by bulk fluidity provided we assume a sticking boundary layer, i.e. a negative velocity slip length of molecular dimensions. The thickness of this boundary layer is discussed with regard to previous capillarity-driven flow experiments (spontaneous imbibition) and with regard to velocity slippage at the pore walls resulting from dissolved gas. Water flow across the silanized, hydrophobic nanopores is blocked up to a hydrostatic pressure of at least 70 bar. The absence of a sticking boundary layer quantitatively accounts for an enhanced n-hexane permeability in the hydrophobic compared to the hydrophilic nanopores.Comment: 15 pages, 7 figures, in press, Physical Review E 201

The Quest for CD8+ Memory Stem Cells

In this issue of Immunity, Turtle et al. (2009) describe the identification of a distinct CD8+ memory T cell subset in humans, which could bring us closer to the identification of the enigmatic “memory stem cell.

Reservoir quality controls on Rotliegend fluvio-aeolian wells in Germany and the Netherlands, Southern Permian Basin – Impact of grain coatings and cements

Reservoir quality in sandstones can be a function of temperature, pressure, and chemical alterations over time. Some intrinsic properties of sandstones can additionally impact the evolution of reservoir quality. The detrital composition, depositional environment, and properties controlled thereby, dictate some of the diagenetic changes. Extrinsic processes, like external fluids, e.g. from hydrothermal events or dewatering of clay minerals, can additionally result in changes of reservoir quality. One of these properties are grain coatings on quartz grains, which can strongly affect pore cementation by quartz overgrowths. To predict and infer the quality of undrilled reservoirs, constraints are needed to predict their occurrence. We analyse two reservoir settings in the Southern Permian Basin and delineate the impact of depositional systems, lithologies, detrital and authigenic composition on reservoir quality and with regard to grain coatings. Results highlight the importance of diagenetic alterations and the source area in regards to burial development in different parts of the Southern Permian Basin. Samples from the Rotliegend Bahnsen Sandstone Member from Germany and the Slochteren Sandstone Formation from the Netherlands have been petrographically and petrophysically analysed and linked to depositional environments. Our data shows that the emplacement and presence of grain coating minerals cannot be simply linked to the depositional environment or processes controlled thereby (e.g. grain size and sorting), neither on a reservoir-, nor on a basin scale. The dissolution of alumosilicates additionally has no consistent impact on the authigenesis of chloritic grain coatings on the reservoir- or basin scale in this study. This implies the necessity of sample specific analyses and importance of the assessment of diagenetic alterations when analysing, modelling, or predicting reservoir quality in similar settings worldwide

Presentation of an Immunodominant Immediate-Early CD8+ T Cell Epitope Resists Human Cytomegalovirus Immunoevasion.

Control of human cytomegalovirus (HCMV) depends on CD8+ T cell responses that are shaped by an individual's repertoire of MHC molecules. MHC class I presentation is modulated by a set of HCMV-encoded proteins. Here we show that HCMV immunoevasins differentially impair T cell recognition of epitopes from the same viral antigen, immediate-early 1 (IE-1), that are presented by different MHC class I allotypes. In the presence of immunoevasins, HLA-A- and HLA-B-restricted T cell clones were ineffective, but HLA-C*0702-restricted T cell clones recognized and killed infected cells. Resistance of HLA-C*0702 to viral immunoevasins US2 and US11 was mediated by the alpha3 domain and C-terminal region of the HLA heavy chain. In healthy donors, HLA-C*0702-restricted T cells dominated the T cell response to IE-1. The same HLA-C allotype specifically protected infected cells from attack by NK cells that expressed a corresponding HLA-C-specific KIR. Thus, allotype-specific viral immunoevasion allows HCMV to escape control by NK cells and HLA-A- and HLA-B-restricted T cells, while the virus becomes selectively vulnerable to an immunodominant population of HLA-C-restricted T cells. Our work identifies a T cell population that may be of particular efficiency in HCMV-specific immunotherapy

Reservoir quality and burial model evaluation by kinetic quartz and illite cementation modeling : Case study of Rotliegendes, north Germany

Silicate reaction kinetics provide a complementary means to other established paleothermal indicators such as organic maturation for evaluating thermal reconstructions. In this study we combine the use of an organic maturation model with kinetic models for quartz and illite cementation to evaluate burial history scenarios for five subsalt wells in lithologically and structurally complex Rotliegendes reservoirs. Models for organic maturation are most sensitive to maximum temperature and provide no direct evidence for the time of peak temperature or the overall duration of high temperatures. By contrast, the kinetics of quartz cementation are much more strongly influenced by the duration of exposure to high temperatures compared with organic indicators. Kinetic models for fibrous illite formation similarly are sensitive to time and temperature and provide predictions for the time of illite formation that can be compared with radiometric dates. Used collectively, these organic and inorganic paleothermal indicators provide improved constraints on thermal evolution compared with conventional approaches. In this study we use these indicators to evaluate two alternative burial history scenarios. Scenario 1 incorporates a hypothesized Jurassic heat flow peak together with significant Late Jurassic deposition and subsequent erosion. Scenario 2 omits the Jurassic heat flow peak and omits the deposition and erosion of the Upper Jurassic. Although both of these scenarios are consistent with organic maturation data, scenario 2 leads to a far better match with quartz cement volumes and fibrous illite K-Ar dates

Neuromuscular Control During Stair Descent and Artificial Tibial Translation After Acute ACL Rupture.

Background Anterior cruciate ligament (ACL) rupture has direct effect on passive and active knee stability and, specifically, stretch-reflex excitability. Purpose/Hypothesis The purpose of this study was to investigate neuromuscular activity in patients with an acute ACL deficit (ACL-D group) compared with a matched control group with an intact ACL (ACL-I group) during stair descent and artificially induced anterior tibial translation. It was hypothesized that neuromuscular control would be impaired in the ACL-D group. Study Design Cross-sectional study; Level of evidence, 3. Methods Surface electromyographic (EMG) activity of the vastus medialis (VM), vastus lateralis (VL), biceps femoris (BF), and semitendinosus (ST) muscles was recorded bilaterally in 15 patients with ACL-D (mean, 13.8 days [range, 7-21 days] since injury) and 15 controls with ACL-I during stair descent and artificially induced anterior tibial translation. The movements of stair descent were divided into preactivity, weight acceptance, and push-off phases. Reflex activity during anterior tibial translation was split into preactivity and short, medium, and late latency responses. Walking on a treadmill was used for submaximal EMG normalization. Kruskal-Wallis test and post hoc analyses with Dunn-Bonferroni correction were used to compare normalized root mean square values for each muscle, limb, movement, and reflex phase between the ACL-D and ACL-I groups. Results During the preactivity phase of stair descent, the hamstrings of the involved leg of the ACL-D group showed 33% to 51% less activity compared with the matched leg and contralateral leg of the ACL-I group (P < .05). During the weight acceptance and push-off phases, the VL revealed a significant reduction (approximately 40%) in the involved leg of the ACL-D group compared with the ACL-I group. At short latency, the BF and ST of the involved leg of the ACL-D group showed a significant increase in EMG activity compared with the uninvolved leg of the ACL-I group, by a factor of 2.2 to 4.6. Conclusion In the acute phase after an ACL rupture, neuromuscular alterations were found mainly in the hamstrings of both limbs during stair descent and reflex activity. The potential role of prehabilitation needs to be further studied

Staging Laparoscopy for Hilar Cholangiocarcinoma: Is it Still Worthwhile?

This study was designed to evaluate the benefit of staging laparoscopy (SL) in patients with suspected hilar cholangiocarcinoma (HCCA) during the past 10 years. Only 50-60% of patients with HCCA who undergo laparotomy are ultimately amenable to a potentially curative resection. In a previous study, we recommended routine use of SL to prevent unnecessary laparotomies. The accuracy of imaging techniques, however, has significantly improved during the past decade, which is likely to impact the yield and accuracy of SL. From 2000 to 2010, 195 patients with suspected HCCA were analyzed. The yield and accuracy of SL were calculated by dividing total number of avoided laparotomies by the total number of laparoscopies or by all patients with unresectable disease, respectively. Factors associated with better yield and accuracy were assessed. Of 195 patients with HCCA, 175 underwent SL. The yield of SL was 14% and the accuracy was 32%. Operative morbidity of SL was 3%, and operative morbidity of laparotomy for unresectable disease was 33%. No clear factors that influenced the yield of SL were found. Overall yield and accuracy of SL for HCCA in the present series decreased to 14% and 32%, respectively, compared with earlier reports. This finding is likely the result of improved imaging techniques that evolved during the past decade. The place of SL in the workup of patients with HCCA needs to be reconsidered, and one should decide whether the declining additional value of SL still outweighs the drawbacks of S

Response to Glyphosate in Alopecurus myosuroides Populations from Lower Saxony

Das Totalherbizid Glyphosat wird weltweit im Pflanzenbau zur Unkrautbekämpfung, Ernteerleichterung, Saatbettbereitung und Abtötung von Zwischenfrüchten eingesetzt. Regelmäßig durchgeführte Gewächshaus-Monitoringversuche zum Auftreten und zur möglichen Verbreitung von Herbizidresistenzen in Niedersachsen zeigten, dass die Wirksamkeit von Glyphosat bei bestimmten Populationen von Alopecurus myosuroides reduziert ist. Dies könnte auf eine mögliche Toleranzverschiebung in Richtung einer reduzierten Sensitivität in manchen Populationen hinweisen. Eine Dosis-Wirkungs-Untersuchung wurde mit sechs Alopecurus myosuroides-Populationen aus verschiedenen Regionen Niedersachsens durchgeführt. Eine Glyphosat-sensitive Referenzpopulation wurde zum Vergleich herangezogen. Die Pflanzen wurden mit einer kommerziell erhältlichen, Glyphosat als Kaliumsalz enthaltenden, Formulierung in aufsteigenden Aufwandmengen (0, 225, 450, 900, 1800, 3600 und 7200 g Glyphosat/ha) behandelt. ED50-Werte wurden aus den Dosis-Wirkungskurven ermittelt und Resistenzfaktoren berechnet. Von sechs geprüften Populationen überlebten drei Wochen nach der Glyphosatbehandlung einzelne Individuen aus vier Populationen bei Aufwandmengen von 1800 und 3600 g Glyphosat/ha ohne jeglichen sichtbaren Schaden und wuchsen weiter. Sechs Wochen nach der Glyphosatbehandlung mit 900 g Glyphosat/ha kam es zu einem Neuaustrieb verschiedener zuvor, als vollständig kontrolliert bewerteter, Individuen aus vier Populationen. Die in dieser Studie gemachten Beobachtungen deuten darauf hin, dass die getesteten Populationen ein Potenzial zur Resistenzentwicklung haben könnten. Weitere Experimente müssen durchgeführt werden, um die zugrundeliegenden Mechanismen zu untersuchen.The broad-scale herbicide glyphosate is used worldwide in crop management system to control weeds, facilitate harvests, prepare seed beds, and desiccate cover crops. Greenhouse monitoring trials on herbicide resistance occurrence and potential spread conducted regularly in Lower Saxony, Germany, revealed a reduced glyphosate efficacy against some populations of Alopecurus myosuroides. This could indicate a potential shift towards a reduced sensitivity in some populations. A dose-response experiment was conducted with six Alopecurus myosuroides populations from various regions of Lower Saxony. A reference population, susceptible to glyphosate, was included for comparison. Plants were treated with a commercially available formulation containing the potassium salt of glyphosate. Treatments were 0, 225, 450, 900, 1800, 3600 and 7200 g glyphosate/ha. ED50 values were determined from the dose-response curves and corresponding resistance factors were calculated. When assessed three weeks after glyphosate treatment, single individuals from four out of six tested populations survived doses of 1800 and 3600 g glyphosate/ha without any visible damage and continued to grow. Six weeks after glyphosate application, a regrowth of several individuals from four populations, previously assessed as completely controlled by 900 g glyphosate/ha, occurred. The observations made in this study indicate that the populations tested may have a potential to evolve glyphosate resistance. Further experiments must be conducted to investigate underlying mechanisms