АНТИБІОТИКОЧУТЛИВІСТЬ КЛІНІЧНИХ ІЗОЛЯТІВ PSEUDOMONAS AERUGINOSA

The results of research of sensitivity to antimicrobial chemotherapy drugs of Pseudomonas aeruginosa isolated from patients with pyo-inflammatory processes of different localization of the urinary tract, ENT-organs, wound contents with mucoviscidosis. For the empirical treatment were revealed the drugs of choice for patients to establish the sensitivity of antimicrobial agents. It has been shown that for an effective treatment could be recommended a limited set of antibiotics, especially, for infections of the urinary tract – piperacillin/tazobactam, cefepim, meropenem, amikacin; for the ENT-organs – piperacillin/tazobactam, cefoperazone, ciprofloxacin, amikacin; piperacillin/tazobactam, ciprofloxacin- when festering; piperacillin/tazobactam, meropenem, cefoperazone – for patients with mucoviscidosis of wound contents.Представлено результати дослідження чутливості до протимікробних хіміотерапевтичних препаратів штамів Pseudomonas aeruginosa, ізольованих від хворих при гнійно-запальних процесах різної локалізації: сечовивідних шляхів, ЛОР-органів, ранового вмісту, хворих на муковісцидоз. Виявлено препарати вибору для емпіричної терапії хворих до встановлення протимікробної чутливості збудників. Показано, що для ефективного лікування може бути рекомендований обмежений набір антибіотиків, насамперед піперацилін/тазобактам, цефепім, меропенем, амікацин при інфекціях сечовивідних шляхів; піперацилін/тазобактам, цефоперазон, ципрофлоксацин, амікацин – ЛОР-органів; піперацилін/тазобактам, ципрофлоксацин – ранового вмісту; піперацилін/тазобактам, меропенем, цефоперазон – при муковісцидозі

Experimental and Theoretical Studies of the C 6 H 5 + C 6 H 6 Reaction

The absolute rate constants for the C 6 H 5 + C 6 H 6 and C 6 D 6 reactions have been measured by cavity ringdown spectrometry at temperatures between 298 and 495 K at a constant 40 Torr Ar pressure. The new results, which reveal no detectable kinetic isotope effect, can be represented by the Arrhenius equation, k 1 ) 10 (11.91(0.13) exp [-(2102 ( 106)/T] cm 3 /(mol s). Our low-temperature data for the addition/stabilization process, C 6 H 5 + C 6 H 6 f C 12 H 11 , can be correlated with those obtained in a low-pressure, high-temperature Knudsen cell study for the addition/displacement reaction, C 6 H 5 + C 6 H 6 f C 12 H 10 + H, by the RRKM theory using the molecular and transition-state parameters computed at the B3LYP/6-311G(d,p) level of theory. Combination of these two sets of data gives k 1 ) 10 (11.98(0.03) exp [-(2168 ( 34)/T] cm 3 /(mol s) covering the temperature range 298-1330 K. The RRKM theory also correlates satisfactorily the forward reaction data with the hightemperature shock-tube result for the reverse H-for-C 6 H 5 substitution process with 2.7 and 4.7 kcal/mol barrier

Reply to S Riehl and K Pustovoytov (Journal of Archaeological Science 33 (2006) 143-144)

We appreciate the interest of Simone Riehl and Konstantin Pustovoytov (hereafter R&P) in our publication and here we answer their critical remarks and questions. R&P criticise two aspects: (1) our interpretation of the pollen record from Kutuzhekovo Lake and (2) the information we derived from the St. Petersburg radiocarbon database. We discuss the questions and we show that these do not really affect our earlier conclusions.

Production of qqQQ final states in ee collisions in the left-right symmetric model

We consider the reaction ee ->qqQQ as a test of lepton number non-conservation in the framework of the left-right-symmetric electroweak model. The main contributions to this process are due to Majorana neutrino exchange in t-channel and doubly charged Higgs (Delta{--}) exchange in s-channel with a pair of right-handed weak bosons (WR) as intermediate state. We show that in a linear ee collider with the collision energy of 1 TeV (1.5 TeV) the cross section of this process is 0.01 fb (1 fb), and it will, for the anticipated luminosity of 10**{35} cm**{-2}, be detectable below the WR threshold. We study the sensitivity of the reaction on the masses of the heavy neutrino, WR and Delta{--}.Comment: 24 pages, 9 eps figures, uses axodraw.st

Рентгенологическое исследование как альтернативный метод визуализации изменений в легких при инфекции, вызванной Covid-19

Introduction. Despite the existence of generally accepted diagnostic protocols, when a new coronavirus infection is suspected, in some cases, it is increasingly difficult to detect changes in the lung tissue in a timely manner due to the heavy workload of the main method of radiation diagnostics – computed tomography. Purpose of the study. To determine the effectiveness of the appointment of an X-ray examination as first-line metgod, as well as to carry out a comparative analysis of the use of radiation diagnostics methods – computed tomography and radiography in relation to the diagnostic sensitivity to changes in lung tissue when a person is infected with the SARS-COV-2 virus.Materials and methods. 150 patients (63.0 ± 8.4 years) with confirmed coronavirus infection were examined. Each of the participants underwent X-ray examination and computed tomography of the chest organs. The percentage of subjects studied for each of the degrees of severity of lung damage was determined to identify the proportion of involvement of lung tissue in the pathological process in the bulk of the examined individuals.Results. Of the 150 patients, changes in the lung tissue during chest X-ray were detected in 97 (65%), respectively, in 53 (35%), pathological changes in the lungs were not visualized. When examining patients by computed tomography, changes in the lungs were detected in 143 patients (95%), X-ray morphological changes were not detected in 7 subjects (5%). When detecting the volume of lung damage, it turned out that the majority of the subjects – 86 people (57%) – had the degree of damage CT-2. The degree of CT-1 and CT-3 was determined in 26 (17%) and 25 (17%) patients, respectively. CT-4 was observed in 6 patients (4%), and in 5% of cases, CT was not able to detect pathological changes in the lung tissue, the degree of CT-0 was established.Conclusion. In the assessment of viral lung damage, radiography takes a significant place, but in 35% of cases, radiographic examination failed to identify the existing pathological changes. CT of the chest organs confirms its value as the “gold standard” in the study of pulmonary pathology in coronavirus infection, but if it is impossible to perform it, radiography is recommended.Введение. Несмотря на наличие общепринятых протоколов диагностики, при подозрении на наличие новой коронавирусной инфекции, в ряде случаев все чаще отмечаются трудности своевременного выявления изменений в легочной ткани ввиду большой загруженности основного метода лучевой диагностики – компьютерной томографии.Цель исследования. Определить эффективность назначения рентгенологического обследования, а также провести сравнительный анализ применения методов лучевой диагностики – компьютерной томографии и рентгенографии в отношении чувствительности к изменению легочной ткани при инфицировании человека вирусом sars-cov-2.Материалы и методы. Было обследовано 150 пациентов (63,0 + 8,4 лет) с подтвержденной коронавирусной инфекцией. Каждому из участников было проведено рентгенологическое обследование и компьютерная томография. Также определен процент исследуемых по каждому из степеней тяжести поражения для выявления процентного соотношения объема вовлечения легких в патологический процесс у основной массы обследуемых лиц.Результаты. Из 150 пациентов изменения легочной ткани посредством проведения рентгена были обнаружены у 97 исследуемых (65%), соответственно у 53 человек (35%) патологические преобразования в легких не визуализировались. При обследовании больных методом компьютерной томографии изменения в легких были выявлены у 143 пациентов (95%), морфологических изменений не было обнаружено у 7 обследуемых (5 %). При выявлении объема поражения легких выяснилось, что основной массе обследуемых - 86 человек (57%) была установлена степень поражения КТ-2.  Степень КТ-1 и КТ-3 определили у 26 (17%) и 25 (17%) пациентов соответственно. КТ-4 наблюдалась у 6 больных (4%), и в 5% случаев КТ не удалось определить патологических изменений в легочной системе, была установлена степень КТ-0.Заключение. В оценке вирусного поражения легких рентгенография занимает существенное место, но в 35% случаев рентгену не удалось идентифицировать патологические изменения. КТ является «золотым стандартом» при исследовании легочной патологии, однако при невозможности проведения компьютерной томографии допускается проведение рентгенографии

Influence of streptococcal arginine deiminase on the leukocyte infiltration in murine air pouch model

Numerous pathogens express arginine deiminase, an enzyme that catalyzes the hydrolysis of L-arginine in a chain of biochemical reactions aimed at the synthesis of ATP in bacterial cells. L-arginine is a semi-essential, proteinogenic amino acid that plays an important role in regulating the functions of the immune system cells in mammals. Depletion of L-arginine may cause a weakening of the immune reaction. In order to improve the conditions of dissemination, many pathogens use a strategy of L-arginine depletion in the microenvironment of host cells. Bacterial arginine deiminase can be a pathogenicity factor aimed for dysregulating the processes of inflammation and immune response. In general, the effect of arginine deiminase on immune cells may result into disturbed production of regulatory proinflammatory molecules, such as NO, and related substances, inhibition of activation, migration and differentiation of individual leukocyte subsets. The aim of this study was to investigate the effect of arginine deiminase on the formation of inflammatory infiltrate in murine air pouch model of streptococcal infection. Materials and methods: The study was performed using S. pyogenes M49-16 expressing arginine deiminase and its isogenic mutant S. pyogenes M49-16delArcA with inactivated arginine deiminase gene. The flow cytometry analysis of the inflammatory infiltrate leukocytes subpopulation in mice infected with the original strain of S. pyogenes M49-16 and its isogenic mutant S. pyogenes M49-16delArcA at different periods of infection was performed. It was shown that the inflammation reached its peak 6 hours after streptococcal inoculation, being more pronounced in mice infected with the mutant strain. Тhis finding was affirmed by a simultaneous and more pronounced increase in the absolute numbers of all leukocyte subsets in the focus of inflammation in this group of mice when compared to mice infected with original bacterial strain. Despite the decrease in the absolute number of all leukocyte types in the inflammatory infiltrate in both groups of mice for 24 hours, this trend was more pronounced in the group of mice infected with mutant microbial strain. Comparison of the inflammatory infiltrates developing in mice infected with original versus mutant strains showed that arginine deiminase may be a pathogenicity factor leading to dysregulation of protective immune response, due to impaired migration of white blood cells to the site of infection

A comparison of methods for purification and concentration of norovirus GII-4 capsid virus-like particles

Noroviruses (NoVs) are one of the leading causes of acute gastroenteritis worldwide. NoV GII-4 VP1 protein was expressed in a recombinant baculovirus system using Sf9 insect cells. Several methods for purification and concentration of virus-like particles (VLPs) were evaluated. Electron microscopy (EM) and histo-blood group antigen (HBGA) binding assays showed that repeated sucrose gradient purification followed by ultrafiltration resulted in intact VLPs with excellent binding to H type 3 antigens. VLPs were stable for at least 12 months at 4°C, and up to 7 days at ambient temperature. These findings indicate that this method yielded stable and high-quality VLPs

ЛЕЧЕНИЕ СЕМИНОМЫ ЯИЧКА I СТАДИИ

Overall survival is about 100% in patients with stage I germinogenic testicular tumors after orchifuniculectomy, which is achieved, by applying alternative adjuvant approaches. The use of approaches, such as a follow-up, chemo- and radiotherapy, may be recommended in seminoma. The paper shows the advantages and disadvantages of these methods.Общая выживаемость пациентов с герминогенными опухолями яичка I стадии после орхифуникулэктомии составляет почти 100%, что достигается благодаря использованию альтернативных адъювантных подходов. При семиноме может быть предложено применение таких методов лечения, как динамическое наблюдение, химио- или лучевая терапия. В статье представлены основные преимущества и недостатки данных методов