Reactivation of contraction in detergent-lysed teleost retinal cones.

Teleost retinal cones contract in the light and elongate in the dark. In the green sunfish, Lepomis cyanellus, the necklike myoid region of the cone contracts from as much as 120 micrometers (midnight dark-adapted) to 6 micrometers in fully light-adapted state. When dark-adapted fish are exposed to light (1.4 lux), cone myoids contract with a linear rate of 1.5 +/- 0.1 micrometers/min. We report here that detergent-lysed motile models of teleost retinal cones exhibit calcium- and ATP-dependent reactivated contraction, with morphology and rate comparable to that observed in vivo. For reactivation studies isolated dark-adapted retinas were lysed with nonionic detergent Brij-58 (0.1-1.0%). In reactivation medium containing 10(-5) M free calcium and 4 mM ATP, the lysed cones contracted with normal morphology at in vivo rates (1.4 +/- 1 micrometer/min). Little contraction was observed if ATP or detergent was deleted from the medium or if free calcium levels were less than 10(-8) M. Ultrastructural examination of cone models lysed with 1% Brij-58 revealed that, in spite of extensive extraction of the cytoplasmic matrix, cytoskeletal components (thin filaments, intermediate filaments, microtubules) were still present. Thus we have produced extensively extracted motile models of teleost retinal cones which undergo calcium- and ATP-dependent reactivated contraction with normal morphology at physiological rate

Modelling and performance analysis of four and eight element TCAS

This semi-annual report describes the work performed during the period September 1989 through March 1990. The first section presents a description of the effect of the engines of the Boeing 737-200 on the performance of a bottom mounted eight-element traffic alert and collision avoidance system (TCAS). The second section deals exclusively with a four element TCAS antenna. The model obtained to simulate the four element TCAS and new algorithms developed for studying its performance are described. The effect of location on its performance when mounted on top of a Boeing 737-200 operating at 1060 MHz is discussed. It was found that the four element TCAS generally does not perform as well as the eight element TCAS III

Design of blended rolled edges for compact range main reflectors

A procedure to design blended rolled edge terminations for arbitrary rim shape compact range main reflectors is presented. The reflector may be center-fed or offset-fed. The design procedure leads to a reflector which has a continuous and smooth surface. This procedure also ensures small diffracted fields from the junction between the paraboloid and the blended rolled edge while satisfying certain constraints regarding the maximum height of the reflector and minimum operating frequency of the system. The prescribed procedure is used to design several reflectors and the performance of these reflectors is presented

Burnside obstructions to the Montesinos-Nakanishi 3-move conjecture

Yasutaka Nakanishi asked in 1981 whether a 3-move is an unknotting operation. In Kirby's problem list, this question is called `The Montesinos-Nakanishi 3-move conjecture'. We define the n-th Burnside group of a link and use the 3rd Burnside group to answer Nakanishi's question; ie, we show that some links cannot be reduced to trivial links by 3-moves.Comment: Published by Geometry and Topology at http://www.maths.warwick.ac.uk/gt/GTVol6/paper11.abs.htm

N-Ethylmaleimide-modified subfragment-1 and heavy meromyosin inhibit reactivated contraction in motile models of retinal cones

The mechanism of contraction in motile models of teleost retinal cones has been examined by using N-ethylmaleimide (NEM)-modified myosin fragments (NEM-S-1 and NEM-heavy meromyosin [HMM]) to prevent access of native myosin to actin filaments during reactivation of contraction. In the diurnal light/dark cycle, retinal cones of green sunfish (Lepomis cyanellus) and bluegill (lepomis macrochirus) exhibit length changes of more than 90 mum. The motile myoid region of the cone contracts from 100 mum in the dark to 6 mum in the light. Motile models for cone contraction have been obtained by lysis of dark-adapted retinas with the non-ionic detergent, Brij-58. These cone motile models undergo Ca(++)-and ATP-dependent reactivated contraction, with morphology and rate comparable to those observed in vivo (Burnside, B.,B. Smith, M. Nagata, and K. Porrello, 1982, J. Cell Biol., 92:198-206). The cone myoids contain longitudinally oriented actin filaments which bind myosin subfragment-1 (S-1) to form characteristic “arrowhead” complexes which dissociate in the presence of MgATP (Burnside, B., 1978, J. Cell Biol., 78:227-246). Modification of S-1 or HMM with the sulfhydryl reagent, NEM, produces new species, NEM-S-1 or NEM-HMM, which still bind actin but which fail to detach in the presence of MgATP (Meeusen, R.L., and W.Z. Cande, 1979, J. Cell Biol., 82:57-65). We have used NEM-S-1 and NEM-HMM to test whether cone contraction depends on an actomyosin force- generating system. We find that reactivated contraction of cone models is inhibited by NEM-S-1 and NEM-HMM but not by the unmodified species, S-1 and HMM. Thus, reactivated cone contraction exhibits NEM-S-1 and NEM-HMM sensitivity as well as Ca(++)- and ATP- dependence. These observations are consistent with and actimyosin-mediated mechanism for force production during cone contraction

Magnetic shielding of long paraboloid structures in the inhomogeneous magnetic field

Abstract: Shielding efficacy of the high-temperature superconducting (HTS) magnetic shields depends on the superconductor properties and on the orientation of the external magnetic field. For precise magnetic field measurements in areas with changing direction of magnetic noise it is important to reduce both the parallel and perpendicular components of the magnetic field. We have designed and fabricated magnetic shields of 25 cm long paraboloid shape with closed sides from second-generation HTS tapes. We have characterized HTS shields in DC and variable frequency AC magnetic fields at 77 K above a copper electromagnet acting as the source of inhomogeneous magnetic noise. The HTS magnetic shields reduce the magnetic field noise penetration and enhance the sensitivity of magnetic field sensors. The measurements were performed with the magnetic shield placed between the noise source and the sensor. 2D finite element analysis using Comsol model was generated and the results were compared with the experimental data of magnetic field dependences of the shielding factor (SF)

Ghosts in modular representation theory

A ghost over a finite p-group G is a map between modular representations of G which is invisible in Tate cohomology. Motivated by the failure of the generating hypothesis---the statement that ghosts between finite-dimensional G-representations factor through a projective---we define the ghost number of kG to be the smallest integer l such that the composition of any l ghosts between finite-dimensional G-representations factors through a projective. In this paper we study ghosts and the ghost numbers of p-groups. We begin by showing that a weaker version of the generating hypothesis, where the target of the ghost is fixed to be the trivial representation k, holds for all p-groups. We then compute the ghost numbers of all cyclic p-groups and all abelian 2-groups with C_2 as a summand. We obtain bounds on the ghost numbers for abelian p-groups and for all 2-groups which have a cyclic subgroup of index 2. Using these bounds we determine the finite abelian groups which have ghost number at most 2. Our methods involve techniques from group theory, representation theory, triangulated category theory, and constructions motivated from homotopy theory.Comment: 15 pages, final version, to appear in Advances in Mathematics. v4 only makes changes to arxiv meta-data, correcting the abstract and adding a do

Assessing the association between oral hygiene and preterm birth by quantitative light-induced fluorescence

The aim of this study was to investigate the purported link between oral hygiene and preterm birth by using image analysis tools to quantify dental plaque biofilm. Volunteers (η = 91) attending an antenatal clinic were identified as those considered to be “at high risk” of preterm delivery (i.e., a previous history of idiopathic preterm delivery, case group) or those who were not considered to be at risk (control group). The women had images of their anterior teeth captured using quantitative light-induced fluorescence (QLF). These images were analysed to calculate the amount of red fluorescent plaque (ΔR%) and percentage of plaque coverage. QLF showed little difference in ΔR% between the two groups, 65.00% case versus 68.70% control, whereas there was 19.29% difference with regard to the mean plaque coverage, 25.50% case versus 20.58% control. A logistic regression model showed a significant association between plaque coverage and case/control status (Ρ = 0.031), controlling for other potential predictor variables, namely, smoking status, maternal age, and body mass index (BMI)