Tear film quality evaluation with intraocular scattering measurements

A new objective method to diagnose and classify dry eye patients has been carried out in this study. Tear film quality was objectively evaluated by means of a double-pass system in patients with healthy eyes, with some tear deficiency and dry eye patients. Intraocular scattering present in those eyes was studied by analyzing a new parameter called Objective Scatter Index (OSI) obtained with the Optical Quality Analysis System (OQAS), a device based on the double-pass technique. Several complementary subjective clinical exams commonly used to evaluate tear film quality were also performed on patients (dry eye questionnaire, Non-Invasive Break-Up Time (NIBUT), Phenol Red Thread Test and Visual Acuity) in order to correlate them with the OSI parameter. Correlations between OSI and dry eye questionnaire and NIBUT showed that there exists a trend that could help to classify more accurately patients affected by some tear proble

Terahertz-based system for dehydration analysis of hydrogel contact lenses

The use of terahertz-based techniques has grown very fast since they are capable of performingevaluations at molecular level, being very suitable for the analysis of biological samples and bioma-terials such as those for contact lenses. These biomaterials are continuously evolving to enhancethe lens wearer’s comfort by improving their hydration state and surface wettability. Therefore, thisstudy examines a novel terahertz system for the assessment of the temporary in vitro dehydrationof hydrogel contact lenses, which provides a new index to assess their state of hydration. Severalconventional and silicone hydrogel contact lenses and lens care solutions were analysed. Traditionalmethods such as the gravimetric determination of water content and the measurement of the staticcontact angle were also carried out for the validation of the developed system. The dehydrationrate measurements of contact lenses obtained with the proposed system correlated with the valuesprovided by traditional methods. As a whole, conventional hydrogel contact lenses exhibited thelowest values for dehydration rate. The tests conducted on various solutions showed a correlationbetween the wetting action of the solution and the dehydration rate of the contact lens material.Peer ReviewedPostprint (published version

Colorimetric analysis of eye fundus structures with multispectral retinography

The analysis of the eye fundus is critical to prevent retinal and choroidal diseases since most of them cause no symptoms at early stages. Treating them when the very first signs appear is crucial to avoid vision losses. To this end, the color of eye fundus structures of healthy and diseased patients was assessed from images acquired with a novel multispectral fundus camera (400 nm – 1300 nm) with high spectral and spatial resolution. Characteristic color traits were found: in healthy eyes, large CIEDE2000 color differences were reported between arteries and veins due to different blood oxygenation; the contrast of nerve fibers/fovea was enhanced, giving rise to relevant color differences; in eyes with age related macular degeneration, lesions such as drusen could be better distinguished than with traditional color retinography; alterations of the optic disk in patients with glaucoma were also assessed, showing remarkable CIEDE2000 values when compared to healthy patientsThis project has received funding from the European Union's Horizon 2020 research and innovation programme under Marie Skłodowska-Curie grant agreement No. 801342 (Tecniospring INDUSTRY) and the Government of Catalonia's Agency for Business Competitiveness (ACCIÓ). This research was also supported by the Ministerio de Economía, Industria y Competitividad (MINECO), the Agencia Estatal de Investigación (AEI) and the European Regional Development Fund (FEDER) under the grant DPI2017-89414-R.Peer ReviewedPostprint (published version

Texture Evaluation of Automotive Coatings by Means of a Gonio-Hyperspectral Imaging System Based on Light-Emitting Diodes

Sparkle and graininess are textural effects that appear as intrinsic spatial features of coatings containing goniochromatic pigments, whereas others such as mottling are undesired outcomes. In this study, we present new methods for the evaluation of sparkle, graininess and mottling of automotive coatings through images acquired with a novel gonio-hyperspectral imaging system based on light-emitting diodes with extended spectral sensitivity beyond the visible range (368–1309 nm). A novel analysis of sparkle that considered the amount of sparkling spots was used. Graininess was quantified through a novel statistical descriptor based on the energy descriptor computed from the histogram. A new index was proposed for an enhanced detection of mottling based on the analysis of striping, which was better observed and quantified in the infrared range. Spectral assessments showed a strong relationship with color and spectral reflectance for graininess and mottling. In conclusion, the results showed that these new spatial and spectral methods are a suitable, alternative and potential approach for the assessment of these textural features.Ministerio de Ciencia e Innovación (MICINN, DPI2011-30090-C02-01) and Ministerio de Economía y Competitividad (MINECO, DPI2014-56850-R); and the European Union

Fast visible and extended nearinfrared multispectral fundus camera

We present a multispectral fundus camera that performs fast imaging of the ocular posterior pole in the visible and near-infrared (400 to 1300 nm) wavelengths through 15 spectral bands, using a flashlight source made of light-emitting diodes, and CMOS and InGaAs cameras. We investigate the potential of this system for visualizing occult and overlapping structures of the retina in the unexplored wavelength range beyond 900 nm, in which radiation can penetrate deeper into the tissue. Reflectance values at each pixel are also retrieved from the acquired images in the analyzed spectral range. The available spectroscopic information and the visualization of retinal structures, specifically the choroidal vasculature and drusen-induced retinal pigment epithelium degeneration, which are hardly visible in conventional color fundus images, underline the clinical potential of this system as a new tool for ophthalmic diagnosis.Peer ReviewedPostprint (published version

Medidas hiperespectrales de fondo de ojo: una nueva herramienta para el diagnóstico oftalmológico

Peer ReviewedPostprint (published version

Graininess characterization by multidimensional scaling

A validation of the graininess attribute was made by means of a psychophysical experiment and the multidimensional scaling algorithm. A visual experiment was designed to obtain graininess differences to be used like the dissimilarity matrix in the MDS algorithm. The results revealed that two dimensions are needed to characterize the graininess effect. The BYK-mac-i instrument and a gonio-hyperspectral imaging system were employed to evaluate the statistical dimensions. On one hand, the first dimension correlated well with the graininess value provided by the BYK-mac-i (r2 = 0.9566). However, we were unable to find a relationship with dimension 2 and any parameter measured by this instrument. Furthermore, the images captured by the gonio-hyperspectral imaging system were processed. A good relationship with the correlation parameter was observed (r2 = 0.8958). However, no relationship was established with dimension 2. Based on these conclusions, further research is necessary that focuses on the new imaging processes and a new visual experiment.The authors are grateful to the EMRP for funding Project ‘Multidimensional Reflectometry for Industry’ (JRPI21) and Project ‘Bidirectional reflectance definitions’ (16NRM08). The EMRP is jointly funded by EMRP participating countries within EURAMET and the European Union. We thank the Spanish Ministry of Economy and Competitiveness for Project DPI2015-65814-R

Morphological study of skin cancer lesions through a 3D scanner based on fringe projection and machine learning

Postprint (published version

Spectroscopic evaluation of red blood cells of thalassemia patients with confocal microscopy: a pilot study

Hemoglobinopathies represent the most common single-gene defects in the world and pose a major public health problem, particularly in tropical countries, where they occur with high frequency. Diagnosing hemoglobinopathies can sometimes be difficult due to the coexistence of different causes of anemia, such as thalassemia and iron deficiency, and blood transfusions, among other factors, and requires expensive and complex molecular tests. This work explores the possibility of using spectral confocal microscopy as a diagnostic tool for thalassemia in pediatric patients, a disease caused by mutations in the globin genes that result in changes of the globin chains that form hemoglobin—in pediatric patients. Red blood cells (RBCs) from patients with different syndromes of alpha-thalassemia and iron deficiency (including anemia) as well as healthy (control) subjects were analyzed under a Leica TCS SP8 confocal microscope following different image acquisition protocols. We found that diseased RBCs exhibited autofluorescence when excited at 405 nm and their emission was collected in the spectral range from 425 nm to 790 nm. Three experimental descriptors calculated from the mean emission intensities at 502 nm, 579 nm, 628 nm, and 649 nm allowed us to discriminate between diseased and healthy cells. According to the results obtained, spectral confocal microscopy could serve as a tool in the diagnosis of thalassemia.This research was funded by Spanish Ministry of Economy and Competitiveness, grant number DPI2017-89414-R. L.R.-B. thanks the Ministry of Science, Innovation and Universities for the PhD (FPI) grant she has received.Peer ReviewedPostprint (published version

Use of spectral information for red scale pest control

Decreasing the use of pesticides is one of the main goals of current agriculture, which requires fast, precise and continuous assessments of crop pests. Citrus pests cause a lot of damage worldwide and the techniques to evaluate them are mainly based on manual, time-consuming readings of insects stuck on traps spread over the crops. This is the case of red scale insects, whose control is notably challenging due to their small size and high reproduction rate. Hence, in this work, we carry out a spectral characterization of this insect in the visible range through spectrometric devices, microscopy and hyperspectral imaging technology to analyze the feasibility of using this information as a means of automatically identifying specimens belonging to this species in this era of precision agriculture. The results obtained show that spectral reflectance differences between red scales and other insects can be recorded at long (red) wavelengths and that red scales are morphologically different, i.e., smaller and more rounded. A reflectance ratio computed from spectral images taken at 774 nm and 410 nm is proposed as a new approach for automated discrimination of red scales from other insects.This project has been co-financed by the European Union through the European Regional Development Fund (ERDF) and has the support of the Secretariat of Universities and Research of the Department of Business and Knowledge of the Generalitat de Catalunya (Exp. 2019PROD00013)Peer ReviewedPostprint (published version