The analysis of the eye fundus is critical to prevent retinal and choroidal diseases since most of them cause no symptoms at early stages. Treating them when the very first signs appear is crucial to avoid vision losses. To this end, the color of eye fundus structures of healthy and diseased patients was assessed from images acquired with a novel multispectral fundus camera (400 nm – 1300 nm) with high spectral and spatial resolution. Characteristic color traits were found: in healthy eyes, large CIEDE2000 color differences were reported between arteries and veins due to different blood oxygenation; the contrast of nerve fibers/fovea was enhanced, giving rise to relevant color differences; in eyes with age related macular degeneration, lesions such as drusen could be better distinguished than with traditional color retinography; alterations of the optic disk in patients with glaucoma were also assessed, showing remarkable CIEDE2000 values when compared to healthy patientsThis project has received funding from the European Union's Horizon 2020 research and innovation programme under Marie Skłodowska-Curie grant agreement No. 801342 (Tecniospring INDUSTRY) and the Government of Catalonia's Agency for Business Competitiveness (ACCIÓ). This research was also supported by the Ministerio de Economía, Industria y Competitividad (MINECO), the Agencia Estatal de Investigación (AEI) and the European Regional Development Fund (FEDER) under the grant DPI2017-89414-R.Peer ReviewedPostprint (published version