Multilayer screen gives cathode ray tube high contrast

Fabrication method for cathode ray tubes uses low-cost siloxane resin formulations. The resins contain sufficient methyl or phenyl groups for solubility in organic solvents. After vaporization and baking, the polymerized material is stable under vacuum and under temperatures required for tube fabrication

Metabolic signature of breast cancer cell line MCF-7: profiling of modified nucleosides via LC-IT MS coupling

<p>Abstract</p> <p>Background</p> <p>Cancer, like other diseases accompanied by strong metabolic disorders, shows characteristic effects on cell turnover rate, activity of modifying enzymes and DNA/RNA modifications, resulting also in elevated amounts of excreted modified nucleosides. For a better understanding of the impaired RNA metabolism in breast cancer cells, we screened these metabolites in the cell culture supernatants of the breast cancer cell line MCF-7 and compared it to the human mammary epithelial cells MCF-10A. The nucleosides were isolated and analyzed via 2D-chromatographic techniques: In the first dimension by cis-diol specific boronate affinity extraction and subsequently by reversed phase chromatography coupled to an ion trap mass spectrometer.</p> <p>Results</p> <p>Besides the determination of ribonucleosides, additional compounds with cis-diol structure, deriving from cross-linked biochemical pathways, like purine-, histidine- and polyamine metabolism were detected. In total, 36 metabolites were identified by comparison of fragmentation patterns and retention time. Relation to the internal standard isoguanosine yielded normalized area ratios for each identified compound and enabled a semi-quantitative metabolic signature of both analyzed cell lines.</p> <p>13 of the identified 26 modified ribonucleosides were elevated in the cell culture supernatants of MCF-7 cells, with 5-methyluridine, <it>N</it>²,<it>N</it>²,7-trimethylguanosine, <it>N</it>⁶-methyl-<it>N</it>⁶-threonylcarbamoyladenosine and 3-(3-aminocarboxypropyl)-uridine showing the most significant differences. 1-ribosylimidazole-4-acetic acid, a histamine metabolite, was solely found in the supernatants of MCF-10A cells, whereas 1-ribosyl-4-carboxamido-5-aminoimidazole and S-adenosylmethionine occurred only in supernatants of MCF-7 cells.</p> <p>Conclusion</p> <p>The obtained results are discussed against the background of pathological changes in cell metabolism, resulting in new perspectives for modified nucleosides and related metabolites as possible biomedical markers for breast carcinoma <it>in vivo</it>.</p

Quality of life in Type 1 (insulin-dependent) diabetic patients prior to and after pancreas and kidney transplantation in relation to organ function

Improvement of the quality of life in Type 1 (insulin-dependent) diabetic patients with severe late complications is one of the main goals of pancreas and/or kidney grafting. To assess the influences of these treatment modalities on the different aspects of the quality of life a cross-sectional study in 157 patients was conducted. They were categorized into patients pre-transplant without dialysis (n=29; Group A), pre-transplant under dialysis (n=44; Group B), post-transplant with pancreas and kidney functioning (n=31; Group C), post-transplant with functioning kidney, but insulin therapy (n=29; Group D), post-transplant under dialysis and insulin therapy again (n=15; Group E) and patients after single pancreas transplantation and rejection, with good renal function, but insulin therapy (n=9; Group F). All patients answered a mailed, self-administered questionnaire (217 questions) consisting of a broad spectrum of rehabilitation criteria. The results indicate a better quality of life in Groups C and D as compared to the other groups. In general the scores are highest in C, but without any significant difference to D. Impressive significant differences between C or D and the other groups were found especially in their satisfaction with physical capacity, leisure-time activities or the overall quality of life. The satisfaction with the latter is highest in C (mean±SEM: 4.0±0.2 on a 1 to 5-rating scale; significantly different from A: 3.1±0.1, B: 2.7±0.2 and E: 2.6±0.3; p<0.01), followed by D (3.8±0.2; significantly different from B and E; p<0.01). Group F shows a mean of 3.1±0.4, which is not significantly different from C. The percentages of patients in each group, who are not working: A: 38 %, B: 64 %, C: 74 %, D: 66 %, E: 87 % and F: 78 % indicate that there is no marked improvement in the vocational situation after successful grafting

Towards Research Collaboration – a Taxonomy of Social Research Network Sites

The increase of scientific collaboration coincides with the technological and social advancement of social software applications which can change the way we research. Among social software, social network sites have recently gained immense popularity in a hedonic context. This paper focuses on social network sites as an emerging application designed for the specific needs of researchers. To give an overview about these sites we use a data set of 24 case studies and in-depth interviews with the founders of ten social research network sites. The gathered data leads to a first tentative taxonomy and to a definition of SRNS identifying four basic functionalities identity and network management, communication, information management, and collaboration. The sites in the sample correspond to one of the following four types: research directory sites, research awareness sites, research management sites and research collaboration sites. These results conclude with implications for providers of social research network sites

Measuring the effects of acupuncture and homoeopathy in general practice: An uncontrolled prospective documentation approach

BACKGROUND: Despite the increasing demand for acupuncture and homoeopathy in Germany, little is known about the effects of these treatments in routine care. We set up a pragmatic documentation study in general practice funded within the scope of project launched by a German health insurer. Patients were followed-up for up to four years. METHODS: The aim of the project was to study the effects and benefits of acupuncture and/or homoeopathy, and to assess patient satisfaction within a prospective documentation of over 5000 acupuncture and over 900 homoeopathy patients. As data sources, we used the documentation made available by therapists on every individual visit and a standardised quality-of-life questionnaire (MOS SF-36); these were complemented by questions concerning the patient's medical history and by questions on patient satisfaction. The health insurer provided us with data on work absenteeism. RESULTS: Descriptive analyses of the main outcomes showed benefit of treatment with middle to large-sized effects for the quality of life questionnaire SF-36 and about 1 point improvement on a rating scale of effects, given by doctors. Data on the treatment and the patients' and physicians' background suggests chronically ill patients treated by fairly regular schemes. CONCLUSION: Since the results showed evidence of a subjective benefit for patients from acupuncture and homoeopathy, this may account for the increase in demand for these treatments especially when patients are chronically ill and unsatisfied with the conventional treatment given previously

A Performance-Based Scenario Methodology to Assess Collaborative Networks Business Model Dynamicity

[EN] In today's business marketplace many enterprises collaborate forming a collaborative network (CN) in order to achieve competitive and sustainable advantages. In this context, CNs should have not only well-defined business models but also mechanisms and tools that help them out to assess such business models as well as other CN operations at their early stages. Due to shorter life-cycles and to the current fierce competition such an evaluation should be made as quickly as possible and analyzing real data rather than based on opinions and subjective judgments. This paper presents the application of a methodology that allows such an assessment as well as the generation of business scenarios based on the performance of the CN. Then, it first defines the appropriate CN key performance indicators (KPIs), gathering data for a certain time-period; then, it applies multivariate techniques to this data, identifying relationships between the KPIs, and being able to build the timely evolution of the CN based on this data; next, it is able to design a business scenario based on the timely evolution that the CN should have according to its business models and operations results achieved so far. With all this additional information decision-makers could decide whether the CN's business models succeeded or not so far and what actions to take in order to achieve the future desirable scenario.This work has been developed within the research project called “Design of business scenarios to improve the efficiency and management of industrial supply chain” (reference GV/2013/045).Rodríguez Rodríguez, R.; Alfaro Saiz, JJ.; Verdecho Sáez, MJ. (2015). A Performance-Based Scenario Methodology to Assess Collaborative Networks Business Model Dynamicity. IFIP Advances in Information and Communication Technology. 463:511-517. https://doi.org/10.1007/978-3-319-24141-8_47S511517463Achtenhagen, L., Melin, L., Naldi, L.: Dynamics of business models – strategizing, critical capabilities and activities for sustained value creation. Long Range Plann. 46, 427–442 (2013)Chesbrough, H.: Business model innovation: opportunities and barriers. Long Range Plann. 43, 354–363 (2010)Chermack, T.J.: Studying scenario planning: theory, research, suggestions, and hypotheses. Technol. Forecast. Soc. Change 72, 59–73 (2005)Harries, C.: Correspondence to what? Coherence to what? What is good scenario-based decision making? Technol. Forecast. Soc. Change 70, 797–817 (2003)Gunasekaran, A., Patel, C., Tirtiroglu, E.: Performance measures and metrics in a supply chain environment. Int. J. Oper. Prod. Manage. 21, 71–87 (2001)Bullinger, H.J., Kühner, M., Hoof, A.V.: Analysing supply chain performance using a balanced measurement method. Int. J. Prod. Res. 40, 3533–3543 (2002)Folan, P., Browne, J.: Development of an extended enterprise performance measurement system. Prod. Plann. Control 16, 531–544 (2005)Fink, A., Marr, B., Siebe, A., Khule, J.-P.: The future scorecard: combining external and internal scenarios to create strategic foresight. Manage. Decis. 43, 360–381 (2005)Othman, R.: Enhancing the effectiveness of the balanced scorecard with scenario planning. Int. J. Prod. Perform. Manage. 57, 259–266 (2008)Rodriguez-Rodriguez, R., Saiz, J.J.A., Bas, A.O., Carot, J.M., Jabaloyes, J.M.: Building internal business scenarios based on real data from a performance measurement system. Technol. Forecast. Soc. Change 77, 50–62 (2010

Transcription factor 4 (TCF4) expression predicts clinical outcome in RUNX1 mutated and translocated acute myeloid leukemia

Contains fulltext : 229528.pdf (publisher's version ) (Open Access

The European DISABKIDS project: development of seven condition-specific modules to measure health related quality of life in children and adolescents

BACKGROUND: The European DISABKIDS project aims to enhance the Health Related Quality of Life (HRQoL) of children and adolescents with chronic medical conditions and their families. We describe the development of the seven cross-nationally tested condition-specific modules of the European DISABKIDS HRQoL instrument in a population of children and adolescents. The condition-specific modules are intended for use in conjunction with the DISABKIDS chronic generic module. METHODS: Focus groups were used to construct the pilot version of the DISABKIDS condition-specific HRQoL modules for asthma, juvenile idiopathic arthritis, atopic dermatitis, cerebral palsy, cystic fibrosis, diabetes and epilepsy. Analyses were conducted on pilot test data in order to construct field test versions of the modules. A series of factor analyses were run, first, to determine potential structures for each condition-specific module, and, secondly, to select a reduced number of items from the pilot test to be included in the field test. Post-field test analyses were conducted to retest the domain structure for the final DISABKIDS condition-specific modules. RESULTS: The DISABKIDS condition-specific modules were tested in a pilot study of 360 respondents, and subsequently in a field test of 1152 respondents in 7 European countries. The final condition-specific modules consist of an 'Impact' domain and an additional domain (e.g. worry, stigma, treatment) with between 10 to 12 items in total. The Cronbach's alpha of the final domains was found to vary from 0.71 to 0.90. CONCLUSION: The condition-specific modules of the DISABKIDS instrument were developed through a step-by-step process including cognitive interview, clinical expertise, factor analysis, correlations and internal consistency. A cross-national pilot and field test were necessary to collect these data. In general, the internal consistency of the domains was satisfactory to high. In future, the DISABKIDS instrument may serve as a useful tool with which to assess HRQoL in children and adolescents with a chronic condition. The condition-specific modules can be used in conjunction with the DISABKIDS chronic generic module

The cell fate determinant Llgl1 influences HSC fitness and prognosis in AML

A unique characteristic of hematopoietic stem cells (HSCs) is the ability to self-renew. Several genes and signaling pathways control the fine balance between self-renewal and differentiation in HSCs and potentially also in leukemia stem cells. Recently, studies have shed light on developmental molecules and evolutionarily conserved signals as regulators of stem cells in hematopoiesis and leukemia. In this study, we provide evidence that the cell fate determinant Llgl1 (lethal giant larvae homolog 1) plays an important role in regulation of HSCs. Loss of Llgl1 leads to an increase in HSC numbers that show increased repopulation capacity and competitive advantage after transplantation. This advantage increases upon serial transplantation or when stress is applied to HSCs. Llgl1−/− HSCs show increased cycling but neither exhaust nor induce leukemia in recipient mice. Llgl1 inactivation is associated with transcriptional repression of transcription factors such as KLF4 (Krüppel-like factor 4) and EGR1 (early-growth-response 1) that are known inhibitors of HSC self-renewal. Decreased Llgl1 expression in human acute myeloid leukemia (AML) cells is associated with inferior patient survival. Thus, inactivation of Llgl1 enhances HSC self-renewal and fitness and is associated with unfavorable outcome in human AML