ESTUDO QUANTITATIVO DO TRÂNSITO EM PONTOS CRÍTICOS DA CIDADE DE JOABAÇA - SC

Os problemas relacionados ao transito nos municípios de Joaçaba, Luzerna e Herval d’Oeste vem se agravando diariamente, acarretando na formação de filas e congestionamento. Desta forma a identificação, quantificação e qualificação dos veículos e fluxos se mostra imprescindível para o desenvolvimento e progresso do meio oeste Catarinense. Para obter estes dados foi realizado estudos de trafego em quatro interseções no centro de Joaçaba, localizadas em pontos críticos, o primeiro na Avenida Santa Teresinha com a Ponte Emílio Baumgard. O segundo ponto é no entroncamento da Av. Santa Teresinha com a Rua Frei Edgar, da qual apresenta grande fluxo de veículos e pedestres devido a Igreja Matriz de Joaçaba e ao Centro Educacional Roberto Trompowsky (CERT). O terceiro ponto é na Av. XV de Novembro com a Rua Getúlio Vargas, uma das principais interseções da cidade, ponto de acesso a Prefeitura Municipal de Joaçaba e pelo apelo comercial dado a avenida. O quarto ponto é na Av. XV de Novembro com a Ponte Jorge Lacerda, interseção com o maior volume de trafego estudado, do qual é trajeto para cidades como Luzerna, Agua Doce, Videira e Herval d´Oeste. Nestes pontos estudados circulam em média 33 mil veículos por dia, representando segundo DENATRAN (2015), 145% da frota joaçabense, ou 80% da frota do arranjo populacional. Com os dados do estudo foi possível atestar fracasso no tipo de transportes em massa na região, dos quais carros e motos representam em média 95% do transito, ocasionando congestionamentos e reduzindo a velocidade

LacaScore: a novel plasma sample quality control tool based on ascorbic acid and lactic acid levels

Introduction Metabolome analysis is complicated by the continuous dynamic changes of metabolites in vivo and ex vivo. One of the main challenges in metabolomics is the robustness and reproducibility of results, partially driven by pre-analytical variations. Objectives The objective of this study was to analyse the impact of pre-centrifugation time and temperature, and to determine a quality control marker in plasma samples. Methods Plasma metabolites were measured by gas chromatography-mass spectrometry (GC–MS) and analysed with the MetaboliteDetector software. The metabolites, which were the most labile to pre-analytical variations, were further measured by enzymatic assays. A score was calculated for their use as quality control markers. Results The pre-centrifugation temperature was shown to be critical in the stability of plasma samples and had a significant impact on metabolite concentration profiles. In contrast, pre-centrifugation delay had only a minor impact. Based on the results of this study, whole blood should be kept on wet ice and centrifuged within maximum 3 h as a prerequisite for preparing EDTA plasma samples fit for the purpose of metabolome analysis. Conclusions We have established a novel blood sample quality control marker, the LacaScore, based on the ascorbic acid to lactic acid ratio in plasma, which can be used as an indicator of the blood pre-centrifugation conditions, and hence the suitability of the sample for metabolome analyses. This method can be applied in research institutes and biobanks, enabling assessment of the quality of their plasma sample collections

Constraining the Kilonova Rate with Zwicky Transient Facility Searches Independent of Gravitational Wave and Short Gamma-ray Burst Triggers

The first binary neutron star merger, GW170817, was accompanied by a radioactivity-powered optical/infrared transient called a kilonova. To date, no compelling kilonova has been found during optical surveys of the sky, independent of gravitational-wave triggers. In this work, we searched the first 23 months of the Zwicky Transient Facility (ZTF) data stream for candidate kilonovae in the form of rapidly evolving transients. We combined ZTF alert queries with forced point-spread-function photometry and nightly flux stacking to increase our sensitivity to faint and fast transients. Automatic queries yielded $>11,200$ candidates, 24 of which passed quality checks and strict selection criteria based on a grid of kilonova models tailored for both binary neutron star and neutron star-black hole mergers. None of the candidates in our sample was deemed a possible kilonova after thorough vetting, catalog cross-matching, and study of their color evolution. The sources that passed our selection criteria are dominated by Galactic cataclysmic variables. In addition, we identified two fast transients at high Galactic latitude, one of which is the confirmed afterglow of long-duration GRB190106A, and the other is a possible cosmological afterglow. Using a survey simulation code, we constrained the kilonova rate for a range of models including top-hat and linearly decaying light curves and synthetic light curves obtained with radiative transfer simulations. For prototypical GW170817-like kilonovae, we constrain the rate to be $R < 1775$ Gpc$^{-3}$ yr$^{-1}$ at 95% confidence level by requiring at least 2 high-significance detections. By assuming a population of kilonovae with the same geometry and composition of GW170817 observed under a uniform viewing angle distribution, we obtained a constraint on the rate of $R < 4029$ Gpc$^{-3}$ yr$^{-1}$.Comment: Submitted for publication in Ap

Constraining the Kilonova Rate with Zwicky Transient Facility Searches Independent of Gravitational Wave and Short Gamma-Ray Burst Triggers

The first binary neutron star merger, GW170817, was accompanied by a radioactivity-powered optical/infrared transient called a kilonova. To date, no compelling kilonova has been found in all-sky optical surveys, independently of short gamma-ray burst and gravitational-wave triggers. In this work, we searched the first 23 months of the Zwicky Transient Facility (ZTF) data stream for candidate kilonovae in the form of rapidly evolving transients. We combined ZTF alert queries with forced point-spread-function photometry and nightly flux stacking to increase our sensitivity to faint and fast transients. Automatic queries yielded >11,200 candidates, 24 of which passed quality checks and selection criteria based on a grid of kilonova models tailored for both binary neutron star and neutron star–black hole mergers. None of the candidates in our sample was deemed a possible kilonova after thorough vetting. The sources that passed our selection criteria are dominated by Galactic cataclysmic variables. We identified two fast transients at high Galactic latitude, one of which is the confirmed afterglow of long-duration GRB 190106A, the other is a possible cosmological afterglow. Using a survey simulation code, we constrained the kilonova rate for a range of models including top-hat, linearly decaying light curves, and synthetic light curves obtained with radiative transfer simulations. For prototypical GW170817-like kilonovae, we constrain the rate to be R < 1775 Gpc⁻³ yr⁻¹ (95% confidence). By assuming a population of kilonovae with the same geometry and composition of GW170817 observed under a uniform viewing angle distribution, we obtained a constraint on the rate of R < 4029 Gpc⁻³ yr⁻¹

Constraining the Kilonova Rate with Zwicky Transient Facility Searches Independent of Gravitational Wave and Short Gamma-Ray Burst Triggers

The first binary neutron star merger, GW170817, was accompanied by a radioactivity-powered optical/infrared transient called a kilonova. To date, no compelling kilonova has been found in all-sky optical surveys, independently of short gamma-ray burst and gravitational-wave triggers. In this work, we searched the first 23 months of the Zwicky Transient Facility (ZTF) data stream for candidate kilonovae in the form of rapidly evolving transients. We combined ZTF alert queries with forced point-spread-function photometry and nightly flux stacking to increase our sensitivity to faint and fast transients. Automatic queries yielded &gt;11,200 candidates, 24 of which passed quality checks and selection criteria based on a grid of kilonova models tailored for both binary neutron star and neutron star-black hole mergers. None of the candidates in our sample was deemed a possible kilonova after thorough vetting. The sources that passed our selection criteria are dominated by Galactic cataclysmic variables. We identified two fast transients at high Galactic latitude, one of which is the confirmed afterglow of long-duration GRB 190106A, the other is a possible cosmological afterglow. Using a survey simulation code, we constrained the kilonova rate for a range of models including top-hat, linearly decaying light curves, and synthetic light curves obtained with radiative transfer simulations. For prototypical GW170817-like kilonovae, we constrain the rate to be Gpc-3 yr-1 (95% confidence). By assuming a population of kilonovae with the same geometry and composition of GW170817 observed under a uniform viewing angle distribution, we obtained a constraint on the rate of R &lt; 4029 Gpc-3 yr-1. © 2020. The American Astronomical Society

SP-D restricts transepithelial HIV-1 passage

Effective prophylactic strategy against the current epidemic of sexually transmitted HIV-1 infection requires understanding of the innate gatekeeping mechanisms at the genital mucosa. Surfactant protein D (SP-D), a member of the collectin family of proteins naturally present in the vaginal tract, is a potential HIV-1 entry inhibitor at the cellular level. Human EpiVaginal tissues compartmentalized in culture inserts were apically exposed to HIV-1 and/or a recombinant fragment of human SP-D (rfhSP-D) and viral passage was assessed in the basal chamber containing mononuclear leukocytes. To map the gene signature facilitating or resisting the transepithelial viral transfer, microarray analysis of the HIV-1 challenged EpiVaginal tissues was performed in the absence or presence of rfhSP-D. Mucosal biocompatibility of rfhSP-D was assessed ex vivo and in the standard rabbit vaginal irritation model. The passage of virus through the EpiVaginal tissues toward the underlying target cells was associated with a global epithelial gene signature including differential regulation of genes primarily involved in inflammation, tight junctions and cytoskeletal framework. RfhSP-D significantly inhibited HIV-1 transfer across the vaginal tissues and was associated with a significant reversal of virus induced epithelial gene signature. Pro-inflammatory NF-κB and mTOR transcripts were significantly downregulated, while expression of the tight junctions and cytoskeletal genes was upheld. In the absence of virus, rfhSP-D directly interacted with the EpiVaginal tissues and upregulated expression of genes related to structural stability of the cell and epithelial integrity. There was no increment in the viral acquisition by the PBMCs present in basal chambers wherein, the EpiVaginal tissues in apical chambers were treated with rfhSP-D. The effective concentrations of rfhSP-D had no effect on lactobacilli, epithelial barrier integrity and were safe on repeated applications onto the rabbit vaginal mucosa. This pre-clinical safety data, coupled with its efficacy of restricting viral passage via reversal of virus-induced gene expression of the vaginal barrier, make a strong argument for clinical trials of rfhSP-D as a topical anti-HIV microbicide.The authors thank HIV Research Trust, UK for providing scholarship to Hrishikesh Pandit and Brigham and Women’s Hospital (BWH) for permitting to visit and work in Fichorova lab. The work was partly supported by Medical Innovation Fund (Project no. 2011-16850) of Indian Council of Medical Research (ICMR), New Delhi, India. We are grateful to Director, NIRRH for providing financial support as the Institutional Grant and all the Institutional facilities for experimentation (Accession no. 618).The authors thank HIV Research Trust, UK for providing scholarship to Hrishikesh Pandit to work at the Fichorova's Laboratory. This study was partly supported by Medical Innovation Fund (Project no. 2011-16850) of Indian Council of Medical Research (ICMR), New Delhi, India. We are grateful to Director, NIRRH for providing financial support via the Institutional Grant and the Institutional facilities (Accession no. 618)

UNIVERSO. Mapas celestes (181). 4° por 1 cm

Notas sobre su utilización. Indica en todo momento el paso de las constelaciones por el meridiano de todos los lugares situados entre el Polo Artico y los 35 grados de Latitud Austra

Blood DNA Yield but Not Integrity or Methylation Is Impacted After Long-Term Storage

Collection of human whole blood for genomic DNA extraction is part of numerous clinical studies. Since DNA extraction cannot always be performed at the time of sample collection, whole blood samples may be stored for years before being processed. The use of appropriate storage conditions is then critical to obtain DNA in sufficient quantity and of adequate quality in order to obtain reliable results from the subsequent molecular biological analyses. In this study, EDTA whole blood samples were collected from 8 healthy volunteers, and different durations (up to 1 year) and temperatures (room temperature, 4°C, -20°C, and -80°C) of storage were compared. The effect of the addition of a DNA preservative agent was also assessed before and after storage. DNA concentrations measured by UV spectrophotometry and spectrofluorometry were used to calculate DNA extraction yields and double-strand DNA ratios. DNA integrity was controlled by agarose gel electrophoresis and long-range polymerase chain reaction. The impact of storage conditions on DNA methylation was also evaluated. Results showed that certain storage conditions have a significant impact on the DNA extraction yield but little or no effect on DNA integrity and methylation. Storage of EDTA blood at -80°C guarantees high-quality DNA with a good yield. Higher DNA extraction yields were obtained with the addition of a DNA preservative agent before thawing EDTA blood stored at -20°C or -80°C. Long-term storage at room temperature in the presence of a DNA preservative agent also appeared to be a reliable procedure

ESTUDO QUANTITATIVO DO TRÂNSITO EM PONTOS CRÍTICOS DA CIDADE DE JOABAÇA - SC

Os problemas relacionados ao transito nos municípios de Joaçaba, Luzerna e Herval d’Oeste vem se agravando diariamente, acarretando na formação de filas e congestionamento. Desta forma a identificação, quantificação e qualificação dos veículos e fluxos se mostra imprescindível para o desenvolvimento e progresso do meio oeste Catarinense. Para obter estes dados foi realizado estudos de trafego em quatro interseções no centro de Joaçaba, localizadas em pontos críticos, o primeiro na Avenida Santa Teresinha com a Ponte Emílio Baumgard. O segundo ponto é no entroncamento da Av. Santa Teresinha com a Rua Frei Edgar, da qual apresenta grande fluxo de veículos e pedestres devido a Igreja Matriz de Joaçaba e ao Centro Educacional Roberto Trompowsky (CERT). O terceiro ponto é na Av. XV de Novembro com a Rua Getúlio Vargas, uma das principais interseções da cidade, ponto de acesso a Prefeitura Municipal de Joaçaba e pelo apelo comercial dado a avenida. O quarto ponto é na Av. XV de Novembro com a Ponte Jorge Lacerda, interseção com o maior volume de trafego estudado, do qual é trajeto para cidades como Luzerna, Agua Doce, Videira e Herval d´Oeste. Nestes pontos estudados circulam em média 33 mil veículos por dia, representando segundo DENATRAN (2015), 145% da frota joaçabense, ou 80% da frota do arranjo populacional. Com os dados do estudo foi possível atestar fracasso no tipo de transportes em massa na região, dos quais carros e motos representam em média 95% do transito, ocasionando congestionamentos e reduzindo a velocidade