21 research outputs found
Phage display as a tool to study human autoantibodies and autoantigens in systemic autoimmune disease. Selection of recombinant (auto)-antibodies specific for human autoantigens in rheumatic disease (RA, SLE, SSc) from human autoimmune-patient and immunized chicken derived phage display libraries
A novel, rapid and simple UPLC–MS/MS method for quantification of favipiravir in human plasma: Application to a bioequivalence study
Selective Laser Sintering Manufacturing and Characterization of Lightweight PA 12 Polymer Composites with Different Hollow Microsphere Additives
The use of additives in polyamide polymeric SLS built frameworks further reinforces the goal of developing
lightweight components, which serves as the basis for the current investigation. In this paper, different
amounts of hollow glass microspheres (HGMs) were added to polyamide 12 (PA 12), and their sintered
components were compared for their physical and mechanical properties, including tensile and 3-point
bending tests. In terms of density reduction, the PA 12-20HGM composite structure achieved the highest
reduction figure of 20.8 %. According to specific strength and modulus calculations, PA 12-20HGS60 and
PA 12-20HGM composite structures provided the highest mechanical test results
Effects of Copper Fillers on Mechanical and Electrical Properties of Selective Laser Sintered PA 12-Cu Composites
Selective Laser Sintering (SLS) is a widely used additive manufacturing (AM) technique for
creating 3D geometries by adding materials in layers. Although neat polymer is mainly used in
powder forms for production, organic or inorganic fillers can be added to produce polymer
composites by SLS method. In this study, Polyamide 12 (PA 12) matrix composite parts filled
with two different copper particles, dendritic and spherical shaped, were produced, and their
mechanical, structural and electrical properties were investigated. The present study outlined
that by increasing incident energy densities during sample fabrication, changes in mechanical
and electrical characteristics were examined. The findings were analysed in terms of filler type
and energy input, which were discovered to have a slight change in the bending behaviour of
SLS components. Furthermore, the impact strength was shown to increase constantly with
increasing energy density. Furthermore, whereas the electrical conductivity of spherical Cufilled
parts rose considerably, no significant change was seen in dendritic-shaped Cu-filled
parts
Oleuropein alleviates malathion-induced oxidative stress and DNA damage in rats
The effects of acute exposure to 250 mg/kg malathion and the protective effects of 20 mg/kg oleuropein, both administered intraperitoneally, were evaluated in Wistar male rats. Malathion administration increased malondialdehyde, nitric oxide, 8-hydroxy-2?-deoxyguanosine, total oxidant status, and DNA damage, yet decreased total antioxidant activity, superoxide dismutase, and catalase activities in blood, liver, and kidney. Administration of oleuropein reversed malathion-induced oxidative stress, lipid peroxidation, DNA damage, and antioxidant enzyme activity. © 2015 Taylor & Francis
Human recombinant anti-La (SS-B) autoantibodies demonstrate the accumulation of phosphoserine-366-containing la isoforms in nucleoplasmic speckles.
Using the recombinant La (SS-B) protein or a phosphorylated peptide derived thereof 27 La-specific human recombinant autoantibodies were selected from anti-La-positive systemic lupus erythematosus and systemic sclerosis patient-derived combinatorial phage display antibody libraries. Binding of these anti-La antibodies to various isoforms of the La protein present in normal and apoptotic cell extracts was analysed by Western blotting. Twenty-four of the selected antibodies recognize most, if not all isoforms of La, whereas three are exclusively reactive with the protein phosphorylated at serine-366. Sequence analysis of the selected antibodies showed a restricted spectrum of diversity in their VH germline gene usage. Remarkably, the recombinant antibodies recognizing exclusively the phosphoserine-366-containing isoform of La displayed a spleckled nucleoplasmic staining pattern in immunofluorescence analysis of HeLa and HEp-2 cells. This pattern differed markedly from those obtained with anti-La antibodies recognizing all isoforms of the La protein. Colocalization experiments with marker antibodies for spliceosomal UsnRNPs and RNA polymerase III subunits revealed that the anti-phosphorylated La antibodies stain the same nucleoplasmic speckles as anti-UsnRNP antibodies. In contrast to anti-UsnRNP antibodies the anti-phosphorylated La antibodies did not stain the Cajal bodies. In addition, no colocalization of phosphorylated La with RNA polymerase III was observed. Potential functional implications of the accumulation of phosphorylated La in nucleoplasmic speckles are discussed
Human recombinant anti-La (SS-B) autoantibodies demonstrate the accumulation of phosphoserine-366-containing la isoforms in nucleoplasmic speckles.
Item does not contain fulltextUsing the recombinant La (SS-B) protein or a phosphorylated peptide derived thereof 27 La-specific human recombinant autoantibodies were selected from anti-La-positive systemic lupus erythematosus and systemic sclerosis patient-derived combinatorial phage display antibody libraries. Binding of these anti-La antibodies to various isoforms of the La protein present in normal and apoptotic cell extracts was analysed by Western blotting. Twenty-four of the selected antibodies recognize most, if not all isoforms of La, whereas three are exclusively reactive with the protein phosphorylated at serine-366. Sequence analysis of the selected antibodies showed a restricted spectrum of diversity in their VH germline gene usage. Remarkably, the recombinant antibodies recognizing exclusively the phosphoserine-366-containing isoform of La displayed a spleckled nucleoplasmic staining pattern in immunofluorescence analysis of HeLa and HEp-2 cells. This pattern differed markedly from those obtained with anti-La antibodies recognizing all isoforms of the La protein. Colocalization experiments with marker antibodies for spliceosomal UsnRNPs and RNA polymerase III subunits revealed that the anti-phosphorylated La antibodies stain the same nucleoplasmic speckles as anti-UsnRNP antibodies. In contrast to anti-UsnRNP antibodies the anti-phosphorylated La antibodies did not stain the Cajal bodies. In addition, no colocalization of phosphorylated La with RNA polymerase III was observed. Potential functional implications of the accumulation of phosphorylated La in nucleoplasmic speckles are discussed
Radiolabeling of morphine with 131I and its biodistribution in rats
PubMed ID: 20707720This study was conducted to determine the possible radiopharmaceutical potential of morphin labeled with 131I. Morphine was extracted from dry capsules of the opium poppy (Papaver somniferum L.), purified by high-performance liquid chromatography, and characterized with nuclear magnetic resonance and infrared spectroscopy. The purified compound was labeled with 131I. Male Albino Wistar rats (18) were used for receptor blockage and unblockage biodistribution studies. Tissue distribution studies showed that radiolabeled morphine had higher uptake in lung, liver, small intestines, large intestines, and stomach than the other tissues. The highest uptake of radiolabeled compounds in rats' brain was found to be in the midbrain and hypothalamus. After receptor blockage with morphine, uptake of 131I-morphine decreased in the lungs, liver, kidney, testis, prostate, spinal cord, cerebellum, hippocampus, striatum, and temporal cortex with respect to receptor unblockage studies of rats. This study concludes that the labeling yield of 131I-morphine was high, high amount of 131I-morphine was found in the hypothalamus, and 131I- morphine has enough stability for diagnostic scanning. © 2010, Mary Ann Liebert, Inc