DNA damage and plasma homocysteine levels are associated with serum metabolites and mineral constituents’ profiles in children with persistent diarrhea

This study describes the association between levels of DNA damage and homocysteine (Hcy) in persistent diarrheic (PD) patients and correlates them with serum biochemical metabolites and mineral components. PD patients (n = 36) age 4 - 6 years from Faisalabad hospitals were examined for anthropometric factors, plasma biochemical and mineral constituents. Compared to 36 normal controls, children with PD had significantly higher concentrations of LDL (p = 0.0001), ALT (p = 0.01),homocysteine (p = 0.001), TOS (p = 0.0001), TBARS (p = 0.001), K (p = 0.0001) and Mg (p = 0.0001) while serum triglyceride, total proteins, albumin, globulin, T3, T4, TAS, Na, Ca, Zn and Cu were significantlylower than those of healthy individuals. Both DNA damage and Hcy were positively linked with LDLcholesterol, TBARS and K (all p values < 0.05). Both Hcy profile and percentage DNA damage in PD patients may impart role in the endothelium damage even in the normal range. PD patients have severe deficiency of macro- and micro-nutrients which may have resulted in enhancement of oxidative stress, DNA damage and Hcy levels in patients’ plasma. Appropriate supplementation of macro- and micronutrients may decrease the DNA damage, Hcy levels and enhance the levels of health markers and decrease the mortality rate of PD patients

Exploration of nutraceutical potential of herbal oil formulated from parasitic plant

Background: Cuscuta reflexa (C. reflexa) is a parasitic climber of medicinal importance. The present study was aimed to evaluate the nutraceutical potential of C. reflexa stems collected from different hosts and to evaluate the role of the herbal formulation in dandruff, hair fall control as well as hair growth promoter.Materials and Methods: Hair formulations of C. reflexa collected from different host plants were prepared in the form of herbal oils (10% w/v). C. reflexa stems were extracted using mustard oil as base oil by using direct boiling technique. Prepared oil was studied as hair tonic. The experimental protocols used were anti-dandruff hair growth activity, as well as hair fall reduction. Herbal hair oils versus mustard oil were evaluated by applying oils on human volunteers with hair fall and dandruff problem whereas promotion of hair growth activity was conducted on rats. The formulated oils were also characterised for proximate analysis, physiochemical composition, as well as antimicrobial activity.Result: The test oils of C. reflexa collected from Azadiracta indica and Zizyphus jujuba were effective in the promotion of hair growth, dandruff control, as well as reduction in hair fall activity.Conclusion: All the formulated oils showed potent antimicrobial activity against all selected strains of bacteria and fungi.Keywords: Cuscuta reflexa, host plants, Hair fall, Dandruff, Combing assay, Herbal hair oil, Hair growth activity

EXPLORATION OF NUTRACEUTICAL POTENTIAL OF HERBAL OIL FORMULATED FROM PARASITIC PLANT

Background: Cuscuta reflexa (C. reflexa) is a parasitic climber of medicinal importance. [pls state study objective] Materials and Methods: Hair formulations of C. reflexa collected from different host plants were prepared in the form of herbal oils (10% w/v). C. reflexa stems were extracted using mustard oil as base oil by using direct boiling technique. Prepared oil was studied as hair tonic. The experimental protocols used were anti-dandruff hair growth activity, as well as hair fall reduction. Herbal hair oils versus mustard oil were evaluated by applying oils on human volunteers with hair fall and dandruff problem whereas promotion of hair growth activity was conducted on rats. The formulated oils were also characterised for proximate analysis, physiochemical composition, as well as antimicrobial activity. Result: The test oils of C. reflexa collected from Azadiracta indica and Zizyphus jujuba were significantly effective in the promotion of hair growth, dandruff control, as well as reduction in hair fall activity. Conclusion: All the formulated oils showed potent antimicrobial activity against all selected strains of bacteria and fungi

A Novel Inhalable Dry Powder to Trigger Delivery of Voriconazole for Effective Management of Pulmonary Aspergillosis

Invasive pulmonary aspergillosis (IPA) is a fatal fungal infection with a high mortality rate. Voriconazole (VCZ) is considered a first-line therapy for IPA and shows efficacy in patients for whom other antifungal treatments have been unsuccessful. The objective of this study was to develop a high-potency VCZ-loaded liposomal system in the form of a dry-powder inhaler (DPI) using the spray-drying technique to convert liposomes into a nanocomposite microparticle (NCMP) DPI, formulated using a thin-film hydration technique. The physicochemical properties, including size, morphology, entrapment efficiency, and loading efficiency, of the formulated liposomes were evaluated. The NCMPs were then examined to determine their drug content, production yield, and aerodynamic size. The L3NCMP was formulated using a 1:1 lipid/L-leucine ratio and was selected for in vitro studies of cell viability, antifungal activity, and stability. These formulated inhalable particles offer a promising approach to the effective management of IPA

Metarhizium brunneum Blastospore Pathogenesis in Aedes aegypti Larvae: Attack on Several Fronts Accelerates Mortality

Aedes aegypti is the vector of a wide range of diseases (e.g. yellow fever, dengue, Chikungunya and Zika) which impact on over half the world's population. Entomopathogenic fungi such as Metarhizium anisopliae and Beauveria bassiana have been found to be highly efficacious in killing mosquito larvae but only now are the underlying mechanisms for pathogenesis being elucidated. Recently it was shown that conidia of M. anisopliae caused stress induced mortality in Ae. aegypti larvae, a different mode of pathogenicity to that normally seen in terrestrial hosts. Blastospores constitute a different form of inoculum produced by this fungus when cultured in liquid media and although blastospores are generally considered to be more virulent than conidia no evidence has been presented to explain why. In our study, using a range of biochemical, molecular and microscopy methods, the infection process of Metarhizium brunneum (formerly M. anisopliae) ARSEF 4556 blastospores was investigated. It appears that the blastospores, unlike conidia, readily adhere to and penetrate mosquito larval cuticle. The blastospores are readily ingested by the larvae but unlike the conidia are able infect the insect through the gut and rapidly invade the haemocoel. The fact that pathogenicity related genes were upregulated in blastospores exposed to larvae prior to invasion, suggests the fungus was detecting host derived cues. Similarly, immune and defence genes were upregulated in the host prior to infection suggesting mosquitoes were also able to detect pathogen-derived cues. The hydrophilic blastospores produce copious mucilage, which probably facilitates adhesion to the host but do not appear to depend on production of Pr1, a cuticle degrading subtilisin protease, for penetration since protease inhibitors did not significantly alter blastospore virulence. The fact the blastospores have multiple routes of entry (cuticle and gut) may explain why this form of the inoculum killed Ae. aegypti larvae in a relatively short time (12-24hrs), significantly quicker than when larvae were exposed to conidia. This study shows that selecting the appropriate form of inoculum is important for efficacious control of disease vectors such as Ae. aegypti

Relapse patterns in NMOSD: evidence for earlier occurrence of optic neuritis and possible seasonal variation

Neuromyelitis optica spectrum disorders (NMOSD) and multiple sclerosis (MS) show overlap in their clinical features. We performed an analysis of relapses with the aim of determining differences between the two conditions. Cases of NMOSD and age- and sex-matched MS controls were collected from across Australia and New Zealand. Demographic and clinical information, including relapse histories, were recorded using a standard questionnaire. There were 75 cases of NMOSD and 101 MS controls. There were 328 relapses in the NMOSD cases and 375 in MS controls. Spinal cord and optic neuritis attacks were the most common relapses in both NMOSD and MS. Optic neuritis (p P = 0.002) were more common in NMOSD and other brainstem attacks were more common in MS (p P = 0.065). Optic neuritis and transverse myelitis are the most common types of relapse in NMOSD and MS. Optic neuritis tends to occur more frequently in NMOSD prior to the age of 30, with transverse myelitis being more common thereafter. Relapses in NMOSD were more severe. A seasonal bias for relapses in spring-summer may exist in NMOSD

Bovine cryptosporidiosis: impact, host-parasite interaction and control strategies

International audienceAbstractGastrointestinal disease caused by the apicomplexan parasite Cryptosporidium parvum is one of the most important diseases of young ruminant livestock, particularly neonatal calves. Infected animals may suffer from profuse watery diarrhoea, dehydration and in severe cases death can occur. At present, effective therapeutic and preventative measures are not available and a better understanding of the host–pathogen interactions is required. Cryptosporidium parvum is also an important zoonotic pathogen causing severe disease in people, with young children being particularly vulnerable. Our knowledge of the immune responses induced by Cryptosporidium parasites in clinically relevant hosts is very limited. This review discusses the impact of bovine cryptosporidiosis and describes how a thorough understanding of the host–pathogen interactions may help to identify novel prevention and control strategies