Treatment of a Complex Distal Triceps Tendon Rupture With a New Technique: A Case Report

Introduction: The distal triceps tendon rupture is an uncommon injury. The acute treatment is well-defined, but when a delayed diagnosis is made or when a tendon retraction is present the alternatives or reconstruction are limited and sometimes complex. Case Presentation: In this case, we report on a 28-year-old man who presented with a chronic disruption of the distal triceps tendon with a gap of approximately 15 cm. The patient was diagnosed in another center with an inveterate breakage of the distal triceps tendon and was initially treated with an Achilles allograft that was complicated by a wound infection and required more than ten surgeries. Nearly 22 months after the initial trauma, and 12 months after the first surgery, we performed a reconstruction with an Achilles tendon allograft using the new technique of distal attachment. At the 12-month follow-up the patient presented a joint balance from -5º to 110º and presented with no pain. Conclusions: The use of an Achilles tendon allograft provides excellent results in complex distal triceps tendon ruptures. We report the use of a new technique to anchor a distal Achilles allograft

Sox9-regulated cell plasticity in colorectal metastasis is attenuated by rapamycin

The cancer stem cell (CSC) hypothesis proposes a hierarchical organization of tumors, in which stem-like cells sustain tumors and drive metastasis. The molecular mechanisms underlying the acquisition of CSCs and metastatic traits are not well understood. SOX9 is a transcription factor linked to stem cell maintenance and commonly overexpressed in solid cancers including colorectal cancer. In this study, we show that SOX9 levels are higher in metastatic (SW620) than in primary colorectal cancer cells (SW480) derived from the same patient. This elevated expression correlated with enhanced self-renewal activity. By gain and loss-of-function studies in SW480 and SW620 cells respectively, we reveal that SOX9 levels modulate tumorsphere formation and self-renewal ability in vitro and tumor initiation in vivo. Moreover, SOX9 regulates migration and invasion and triggers the transition between epithelial and mesenchymal states. These activities are partially dependent on SOX9 post-transcriptional modifications. Importantly, treatment with rapamycin inhibits self-renewal and tumor growth in a SOX9- dependent manner. These results identify a functional role for SOX9 in regulating colorectal cancer cell plasticity and metastasis, and provide a strong rationale for a rapamycin-based therapeutic strategy.published_or_final_versio

Effect of resveratrol on alcohol-induced mortality and liver lesions in mice

BACKGROUND: Resveratrol is a polyphenol with important antiinflammatory and antioxidant properties. We investigated the effect of resveratrol on alcohol-induced mortality and liver lesions in mice. METHODS: Mice were randomly distributed into four groups (control, resveratrol-treated control, alcohol and resveratrol-treated alcohol). Chronic alcohol intoxication was induced by progressively administering alcohol in drinking water up to 40% v/v. The mice administered resveratrol received 10 mg/ml in drinking water. The animals had free access to standard diet. Blood levels were determined for transaminases, IL-1 and TNF-α. A histological evaluation was made of liver damage, and survival among the animals was recorded. RESULTS: Transaminase concentration was significantly higher in the alcohol group than in the rest of the groups (p < 0.05). IL-1 levels were significantly reduced in the alcohol plus resveratrol group compared with the alcohol group (p < 0.05). TNF-α was not detected in any group. Histologically, the liver lesions were more severe in the alcohol group, though no significant differences between groups were observed. Mortality in the alcohol group was 78% in the seventh week, versus 22% in the alcohol plus resveratrol group (p < 0.001). All mice in the alcohol group died before the ninth week. CONCLUSION: The results obtained suggest that resveratrol reduces mortality and liver damage in mice

The origins of Valdepeñas (Ciudad Real): the roman vicus and the medieval deserted Aberturas. Historical and archaeological research in La Mancha

Con motivo de la ejecución de obras en la autovía A4 han sido descubiertos e investigados elementos arqueológicos muebles e inmuebles en el yacimiento de Aberturas (Valdepeñas, Ciudad Real). Los materiales recuperados ponen de manifiesto la existencia de un extenso vicus de aproximadamente 24 hectáreas fundado en la segunda mitad del siglo III d.C. Este asentamiento ha tenido un largo recorrido histórico desde entonces hasta hoy. En ese lugar se han desarrollado las poblaciones de Aberturas (denominación medieval y moderna), Consolación (denominación moderna y contemporánea) y Villanueva de Franco (denominación contemporánea). Además de viviendas y materiales arqueológicos cerámicos romanos, medievales -tanto islámicos como cristianos- y modernos, la intervención arqueológica ha permitido documentar silos, varios de los cuales fueron hallados vacíos y cerrados. También se han encontrado un pozo antiguo que explotaba el Acuífero 23, piletas, un lagar y el locus de otroOn the occasion of works on the A4 Highway have been discovered and researched an archaeological site at Aberturas (Valdepeñas, Ciudad Real). Recovered materials reveal the existence of a large vicus with approximately 24 hectares, founded in the second half of the third century AD. This settlement has had a long history since then until today, because of the development of populations called Aberturas (the medieval and modern name), Consolation (the modern and contemporary name) or Villanueva de Franco (the contemporary name). The archaeological intervention let document houses, pottery, glass, silos -several of these empty and closed-, a well, pools, a winery and a locus of another on

La restauración de la villa romana de La Ontavia (Terrinches, Ciudad Real)

En el mes de julio de 2010 se iniciaron las labores de conservación y restauración en el yacimiento romano de La Ontavia (Terrinches, Ciudad Real). Durante cuatro meses se han simultaneado estos trabajos con la excavación arqueológica, en la que han colaborado once alumnos de la Escuela Superior de Conservación y Restauración de Bienes Culturales de la Comunidad de Madrid, bajo la dirección del equipo técnico, realizando prácticas para completar su formación. La intervención llevada a cabo ha estado enfocada a minimizar el efecto de los agentes de deterioro a los que se halla expuesto el yacimiento, que se encuentra al aire libre. El presente artículo tiene como finalidad la difusión de los trabajos de conservación y restauración realizados durante esta campaña, prestando especial atención a la metodología y a los materiales utilizadosIn July 2010 conservation and restoration work began at the Roman site of La Ontavia (Terrinches, Ciudad Real). For four months, the work was carried out alongside the archaeological excavation, involving eleven students from the Madrid School of Cultural Asset Conservation and Restoration, doing work experience under the guidance of a technical team to complete their training. The intervention focused on minimising the effect of the deterioration factors that this open-air site has been exposed to. This article aims to provide a report of the conservation and restoration work carried out in this campaign, paying special attention to the methodology and materials use

Effect of aspirin on the diagnostic accuracy of the faecal immunochemical test for colorectal advanced neoplasia

Background: Aspirin (ASA) is a drug that can cause gastrointestinal lesions and symptoms. Colorectal cancer (CRC) is the most prevalent type of cancer in Western countries. We assessed the effect of aspirin on the diagnostic accuracy of the faecal immunochemical test (FIT) for CRC and/or advanced neoplasia (AN) in patients undergoing colonoscopy for gastrointestinal symptoms. Methods: We conducted a prospective multicentre observational study of diagnostic tests that included patients with gastrointestinal symptoms undergoing colonoscopy between March 2012 and 2014 (the COLONPREDICT study). Symptoms were assessed and a FIT and blood tests assessing haemoglobin and carcinoembryonic antigen (CEA) levels were performed. Results: The study included 3052 patients: A total of 2567 did not take aspirin (non-user group) and 485 (16%) took aspirin (user group). Continuous treatment with ASA did not change the AUC (0.88, 0.82; p = 0.06), sensitivity (92%, 88%; p = 0.5) or specificity (71%, 67%; p = 0.2) of the FIT for CRC detection. Similarly, we found no differences in the AUC (0.81, 0.79; p = 0.6), sensitivity (74%, 75.5%; p = 0.3) or specificity (76%, 73.6%; p = 0.3) for AN detection. Patients with an aspirin use of = 300 mg/day had a lower prevalence of AN and the sensitivity, specificity and AUC for AN for these patients were 54%, 68% and 0.66, significantly lower than for the non-user group (p = 0.03). Conclusions: Aspirin does not modify the diagnostic accuracy of FIT for CRC and/or AN in patients with gastrointestinal symptoms. Aspirin use of = 300 mg/day decreases the accuracy of the test

Accuracy of single molecular biomarkers in gingival crevicular fluid for the diagnosis of periodontitis: A systematic review and meta-analysis

This is the peer reviewed version of the following article: Arias‐Bujanda, N, Regueira‐Iglesias, A, Balsa‐Castro, C, Nibali, L, Donos, N, Tomás, I. Accuracy of single molecular biomarkers in gingival crevicular fluid for the diagnosis of periodontitis: A systematic review and meta‐analysis. J Clin Periodontol. 2019; 46: 1166– 1182. https://doi.org/10.1111/jcpe.13188, which has been published in final form at https://doi.org/10.1111/jcpe.13188. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived VersionsAbstract Aim To analyse, by means of a meta‐analytical approach, the diagnostic accuracy of molecular biomarkers in gingival crevicular fluid (GCF) for the detection of periodontitis in systemically healthy subjects. Material and Methods Studies on GCF molecular biomarkers providing a binary classification table (or sensitivity and specificity values and group sample sizes) in individuals with clinically diagnosed periodontitis were considered eligible. The search was performed using six electronic databases. The methodological quality of studies was assessed through the tool Quality Assessment of Diagnostic Studies. Meta‐analyses were performed using the Hierarchical Summary Receiver Operating Characteristic, which adjusts classification data using random effects logistic regression. Results The included papers identified 36 potential biomarkers for the detection of periodontitis and for four of them meta‐analyses were performed. The median sensitivity and specificity were for MMP8, 76.7% and 92.0%; for elastase, 74.6% and 81.1%; for cathepsin, 72.8% and 67.3%, respectively. The worst estimates of sensitivity and specificity were for trypsin (71.3% and 66.1%, respectively). Conclusions MMP8 showed good sensitivity and excellent specificity, which resulted in this biomarker being clinically the most useful or effective for the diagnosis of periodontitis in systemically healthy subjects, regardless of smoking conditionInstituto de Salud Carlos III FEDER. Grant Number: ISCIII/PI17/01722 Consellería de Cultura, Educación e Ordenación Universitaria da Xunta de Galicia. Grant Numbers: ED431B 2017/029, ED481A‐201

Análisis bibliométrico de las revistas españolas de Prehistoria y Arqueología en los últimos diez años

The paper explains some issues concerning scientific activity in Prehistory and Archaeology through the citation analysis from Spanish periodicals and other factors. It states the difficulties caused by the journals' systematic non-observance of their periodicity and their noticeable lack of bibliographical citation stardardization. That situation harms the contribution of Spanish journals to the international networks of citation and leads to ostracism of Spanish scientific production. Some data concerning co-authorship, theme and language are offered. The analysis of the importance of territory in the cited journals shows that the number of citations diminishes when the distance to the place of publication increases. The relevance of citation analysis as a methodological criteria for periodicals evaluation is also considered. It is concluded that the localism provoked by the descriptive trend of archaeological research has two serious risks: 1) the division of the scientific community into disconnected groups, and 2) the use of very contemporary demarcation lines as a relevant tool for the study of the past.El artículo da cuenta de ciertos aspectos de la actividad científica en Prehistoria y Arqueología a través del análisis de citas desde revistas españolas entre otros factores. Plantea las dificultades derivadas del incumplimiento sistemático de la periodicidad y la notable falta de normalización en la recogida de referencias bibliográficas por parte de las revistas, lo que dificulta la participación de las publicaciones españolas en los circuitos internacionales de citas y contribuye al ostracismo de la producción científica española. Se ofrecen datos sobre coautoría, temática y lengua. Se analiza la importancia del territorio advirtiendo que entre las revistas citadas la intensidad de citas decrece con la distancia a la región de publicación. Asimismo, se valora el interés de los análisis de citas como criterio metodológico en la evaluación de las publicaciones periódicas. Se concluye que el localismo provocado por la orientación descriptiva de la investigación tiene dos graves riesgos: 1) la compartimentación de la comunidad científica en grupos desconectados y 2) una conceptualización actualista del pasado

Effect of oral anticoagulants on the outcome of faecal immunochemical test

Background: We aimed to evaluate whether oral anticoagulants (OACs) alter faecal immunochemical test (FIT) performance in average-risk colorectal cancer (CRC) screening. Methods: Individuals aged 50–69 years were invited to receive one FIT sample (cutoff 75¿ng¿ml–1) between November 2008 and June 2011. Results: Faecal immunochemical test was positive in 9.3% (21 out of 224) of users of OAC and 6.2% (365 out of 5821) of non-users (P-trend=0.07). The positive predictive value (PPV) for advanced neoplasia (AN) in non-users was 50.4% vs 47.6% in users (odds ratio, 0.70; 95% CI, 0.3–1.8; P=0.5). The PPV for AN in OAC more antiplatelets (aspirin or clopidogrel) was 75% (odds ratio, 2; 95% CI, 0.4–10.8; P=0.4). Conclusions: Oral anticoagulant did not significantly modify the PPV for AN in this population-based colorectal screening program. The detection rate of advanced adenoma was higher in the combination OAC more antiplatelets

Synthetic Conjugates of Ursodeoxycholic Acid Inhibit Cystogenesis in Experimental Models of Polycystic Liver Disease

Altres ajuts: Suported by IKERBASQUE, Basque foundation for Science (M.J. Perugorria and J.M. Banales), Spain; "Junta de Castilla y Leon" (J.J.G. Marin: SA06P17); "Diputación Foral Gipuzkoa" (J.M. Banales: DFG15/010, DFG16/004; M.J. Perugorria: DFG18/114, DFG19/081), BIOEF (Basque Foundation for Innovation and Health Research: EiTB Maratoia BIO15/CA/016/BD to J.M. Banales), Department of Health of the Basque Country (J.M. Banales: 2017111010; M.J. Perugorria: 2019111024), and Euskadi RIS3 (J.M. Banales: 2016222001, 2017222014, and 2018222029; 2019222054); La Caixa Scientific Foundation (J.M. Banales: HR17-00601); "Fundación Científica de la Asociación Española Contra el Cáncer" (AECC Scientific Foundation, to J.M. Banales and J.J.G. Marin); and "Centro Internacional sobre el Envejecimiento", Spain (J.J.G. Marin: OLD-HEPAMARKER, 0348-CIE-6-E). A. Santos-Laso by the Basque Government (PRE_2018_2_0195), and Pui Y. Lee-Law by the European Association for the Study of the Liver (EASL; Sheila Sherlock Award). Basque Government (F.P. Cossío: IT-324-07). I. Rivilla had a postdoctoral contract from the Donostia International Physics Center.Background and Aims: Polycystic liver diseases (PLDs) are genetic disorders characterized by progressive development of symptomatic biliary cysts. Current surgical and pharmacological approaches are ineffective, and liver transplantation represents the only curative option. Ursodeoxycholic acid (UDCA) and histone deacetylase 6 inhibitors (HDAC6is) have arisen as promising therapeutic strategies, but with partial benefits. Approach and Results: Here, we tested an approach based on the design, synthesis, and validation of a family of UDCA synthetic conjugates with selective HDAC6i capacity (UDCA-HDAC6i). Four UDCA-HDAC6i conjugates presented selective HDAC6i activity, UDCA-HDAC6i #1 being the most promising candidate. UDCA orientation within the UDCA-HDAC6i structure was determinant for HDAC6i activity and selectivity. Treatment of polycystic rats with UDCA-HDAC6i #1 reduced their hepatomegaly and cystogenesis, increased UDCA concentration, and inhibited HDAC6 activity in liver. In cystic cholangiocytes UDCA-HDAC6i #1 restored primary cilium length and exhibited potent antiproliferative activity. UDCA-HDAC6i #1 was actively transported into cells through BA and organic cation transporters. Conclusions: These UDCA-HDAC6i conjugates open a therapeutic avenue for PLDs