Lymphatic endothelium stimulates melanoma metastasis and invasion via MMP14-dependent Notch3 and b1-integrin activation

Lymphatic invasion and lymph node metastasis correlate with poor clinical outcome in melanoma. However, the mechanisms of lymphatic dissemination in distant metastasis remain incompletely understood. We show here that exposure of expansively growing human WM852 melanoma cells, but not singly invasive Bowes cells, to lymphatic endothelial cells (LEC) in 3D co-culture facilitates melanoma distant organ metastasis in mice. To dissect the underlying molecular mechanisms, we established LEC co-cultures with different melanoma cells originating from primary tumors or metastases. Notably, the expansively growing metastatic melanoma cells adopted an invasively sprouting phenotype in 3D matrix that was dependent on MMP14, Notch3 and β1-integrin. Unexpectedly, MMP14 was necessary for LEC-induced Notch3 induction and coincident β1-integrin activation. Moreover, MMP14 and Notch3 were required for LEC-mediated metastasis of zebrafish xenografts. This study uncovers a unique mechanism whereby LEC contact promotes melanoma metastasis by inducing a reversible switch from 3D growth to invasively sprouting cell phenotype

Role network project to attraction adult population for sports and recreational activities sports facilities

In the work the potential educational impact of physical culture and national sports as factor of increase of health preservation and physical cultureВ работе раскрывается потенциал воспитательного воздействия физкультурно-оздоровительной работы и национальных видов спорта как фактора повышения здоровьесбережения и физической культур

Productivity of peppermint varieties (menta piperita) when grown in vitro using hydroponics

The article describes the method of hydroponics, explores the history of the origin of hydroponics and also describes a plan for the future practical part of the work aimed at analyzing the productivity of peppermint varieties (Menta piperita) when grown in vitro using the method of hydroponics.В статье рассмотрен метод гидропоники, история происхождения гидропоники, а также описан план будущей практической части работы, направленной на анализ продуктивности сортов мяты перечной (Menta piperita) при выращивании in vitro методом гидропоники

Productivity of peppermint varieties (menta piperita) when grown in vitro using hydroponics

The article describes the method of hydroponics, explores the history of the origin of hydroponics and also describes a plan for the future practical part of the work aimed at analyzing the productivity of peppermint varieties (Menta piperita) when grown in vitro using the method of hydroponics.В статье рассмотрен метод гидропоники, история происхождения гидропоники, а также описан план будущей практической части работы, направленной на анализ продуктивности сортов мяты перечной (Menta piperita) при выращивании in vitro методом гидропоники

The use of innovative assessment tools in the process of physical education students at the university

The article deals with the problem of fund formation evaluation meansВ статье рассматриваются проблемы формирования фонда оценочных средст

Productivity of peppermint varieties (menta piperita) when grown in vitro using hydroponics

The article describes the method of hydroponics, explores the history of the origin of hydroponics and also describes a plan for the future practical part of the work aimed at analyzing the productivity of peppermint varieties (Menta piperita) when grown in vitro using the method of hydroponics.В статье рассмотрен метод гидропоники, история происхождения гидропоники, а также описан план будущей практической части работы, направленной на анализ продуктивности сортов мяты перечной (Menta piperita) при выращивании in vitro методом гидропоники

Characterising long COVID: a living systematic review

BACKGROUND: While it is now apparent clinical sequelae (long COVID) may persist after acute COVID-19, their nature, frequency and aetiology are poorly characterised. This study aims to regularly synthesise evidence on long COVID characteristics, to help inform clinical management, rehabilitation strategies and interventional studies to improve long-term outcomes. METHODS: A living systematic review. Medline, CINAHL (EBSCO), Global Health (Ovid), WHO Global Research on COVID-19 database, LitCovid and Google Scholar were searched till 17 March 2021. Studies including at least 100 people with confirmed or clinically suspected COVID-19 at 12 weeks or more post onset were included. Risk of bias was assessed using the tool produced by Hoy et al. Results were analysed using descriptive statistics and meta-analyses to estimate prevalence. RESULTS: A total of 39 studies were included: 32 cohort, 6 cross-sectional and 1 case-control. Most showed high or moderate risk of bias. None were set in low-income countries and few included children. Studies reported on 10 951 people (48% female) in 12 countries. Most included previously hospitalised people (78%, 8520/10 951). The longest mean follow-up time was 221.7 (SD: 10.9) days post COVID-19 onset. Over 60 physical and psychological signs and symptoms with wide prevalence were reported, most commonly weakness (41%; 95% CI 25% to 59%), general malaise (33%; 95% CI 15% to 57%), fatigue (31%; 95% CI 24% to 39%), concentration impairment (26%; 95% CI 21% to 32%) and breathlessness (25%; 95% CI 18% to 34%). 37% (95% CI 18% to 60%) of patients reported reduced quality of life; 26% (10/39) of studies presented evidence of reduced pulmonary function. CONCLUSION: Long COVID is a complex condition with prolonged heterogeneous symptoms. The nature of studies precludes a precise case definition or risk evaluation. There is an urgent need for prospective, robust, standardised, controlled studies into aetiology, risk factors and biomarkers to characterise long COVID in different at-risk populations and settings. PROSPERO REGISTRATION NUMBER: CRD42020211131