12 research outputs found
Prediction of preterm birth with and without preeclampsia using mid-pregnancy immune and growth-related molecular factors and maternal characteristics.
OBJECTIVE:To evaluate if mid-pregnancy immune and growth-related molecular factors predict preterm birth (PTB) with and without (±) preeclampsia. STUDY DESIGN:Included were 400 women with singleton deliveries in California in 2009-2010 (200 PTB and 200 term) divided into training and testing samples at a 2:1 ratio. Sixty-three markers were tested in 15-20 serum samples using multiplex technology. Linear discriminate analysis was used to create a discriminate function. Model performance was assessed using area under the receiver operating characteristic curve (AUC). RESULTS:Twenty-five serum biomarkers along with maternal age <34 years and poverty status identified >80% of women with PTB ± preeclampsia with best performance in women with preterm preeclampsia (AUC = 0.889, 95% confidence interval (0.822-0.959) training; 0.883 (0.804-0.963) testing). CONCLUSION:Together with maternal age and poverty status, mid-pregnancy immune and growth factors reliably identified most women who went on to have a PTB ± preeclampsia
Insights in the ecology and evolutionary history of the Miscellaneous Crenarchaeotic Group lineage
Este artĂculo contiene 13 páginas, 7 figuras, 1 tabla.Members of the archaeal Miscellaneous Crenarchaeotic Group (MCG) are among the most successful
microorganisms on the planet. During its evolutionary diversification, this very diverse group has
managed to cross the saline–freshwater boundary, one of the most important evolutionary barriers
structuring microbial communities. However, the current understanding on the ecological
significance of MCG in freshwater habitats is scarce and the evolutionary relationships between
freshwater and saline MCG remains poorly known. Here, we carried out molecular phylogenies using
publicly available 16S rRNA gene sequences from various geographic locations to investigate the
distribution of MCG in freshwater and saline sediments and to evaluate the implications of saline–
freshwater transitions during the diversification events. Our approach provided a robust ecological
framework in which MCG archaea appeared as a core generalist group in the sediment realm.
However, the analysis of the complex intragroup phylogeny of the 21 subgroups currently forming the
MCG lineage revealed that distinct evolutionary MCG subgroups have arisen in marine and
freshwater sediments suggesting the occurrence of adaptive evolution specific to each habitat. The
ancestral state reconstruction analysis indicated that this segregation was mainly due to the
occurrence of a few saline–freshwater transition events during the MCG diversification. In addition, a
network analysis showed that both saline and freshwater MCG recurrently co-occur with archaea of
the class Thermoplasmata in sediment ecosystems, suggesting a potentially relevant trophic
connection between the two clades.This research was funded by projects ARCANOX (ref.
CGL2009-13318-C02-02) and ARCOS (ref. CGL2012-33033)
to CMB, and DARKNESS CGL2012-32747 to EOC from the
Spanish Ministry of Economy and Competitiveness
(MINECO). J-CA was funded by project METMIC from
the Pyrenean Work Community and the Aquitaine region.Peer reviewe