Foodstuffs - Simultaneous Determination of Nine Sweeteners by High Performance Liquid Chromatography and Evaporate Light Scattering Detection

No abstract available since it is a standard operationg procedure for a validated testing method !!JRC.D.8-Food safety and qualit

Validation of an Analytical Method for the Simultaneous Determination of Nine Intense Sweeteners by HPLC-ELSD

A collaborative trial was conducted to validate an analytical method for the simultaneous determination of nine intense sweeteners, i.e., acesulfame-K, alitame, aspartame, cyclamic acid, dulcin, neotame, neohesperidine dihydrochalcone, saccharin and sucralose in carbonated and non-carbonated soft drinks, and canned or bottled fruits. The procedure involves an extraction of the nine sweeteners with a buffer solution, sample clean-up using solid-phase extraction cartridges followed by an HPLC-ELSD analysis. Trueness, expressed in terms of recovery rates, was demonstrated in most cases by values ranging from 90 to 108%. High comparability of results obtained by individual testing laboratories was ensured by RSDR values <10% for the majority of results. Moreover, HorRAT values of less than 1.1 suggested for all sweeteners and matrices tested good performance of the method.JRC.D.8-Food safety and qualit

CERTIFICATION REPORT: The certification of the cold filter plugging point (CFPP) and cloud point (CP) in automotive diesel fuel containing a volume fraction of 7 % biodiesel: ERM®-EF004

This report describes the production of ERM®-EF004, which is an automotive diesel fuel material containing a volume fraction of 7 % fatty acid methyl ester (biodiesel) certified for the cold filter plugging point and the cloud point. This material was produced following ISO Guide 34:2009 and is certified in accordance with ISO Guide 35:2006. The material is an automotive diesel fuel containing a volume fraction of approximately 7 % biodiesel that is based on rapeseed oil fatty acid methyl ester with the addition of 1 g/kg antioxidant (butylhydroxytoluene). It was provided by a producer in Germany. The material was filled in amber glass ampoules. Between unit-homogeneity was quantified and stability during dispatch and storage were assessed in accordance with ISO Guide 35:2006 [ ]. Within-unit homogeneity was not quantified as the minimum sample intake is defined by the required sample volume stipulated in the respective documentary standard and comprises almost the whole volume of the two ampoules of every unit. The material was characterised by an interlaboratory comparison of laboratories of demonstrated competence and adhering to ISO/IEC 17025:2005). Technically invalid results were removed but no outlier was eliminated on statistical grounds only. Uncertainties of the certified values were calculated in accordance with the Guide to the Expression of Uncertainty in Measurement (GUM) [ ] and include uncertainties related to possible inhomogeneity, instability and characterisation. The material is intended for quality control and assessment of method performance. As with any reference material, it can be used for establishing control charts or validation studies. The certified reference material (CRM) is available in sets of two amber glass ampoules, each containing 27 mL of automotive diesel fuel material with a volume fraction of 7 % biodiesel closed under argon atmosphere.JRC.F.6-Reference Material

CERTIFICATION REPORT: The certification of selected chemical and physical properties in automotive diesel fuel containing a volume fraction of 7 % biodiesel: ERM®-EF003

This report describes the production of ERM®-EF003, which is an automotive diesel fuel material containing a volume fraction of 7 % fatty acid methyl ester (biodiesel) certified for the volume fraction of the fatty acid methyl ester content, the mass fraction of mono-aromatic hydrocarbon, di-aromatic hydrocarbon, polycyclic aromatic hydrocarbon, and total aromatic hydrocarbon content, and density, kinematic viscosity, and lubricity. This material was produced following ISO Guide 34:2009 [ ] and is certified in accordance with ISO Guide 35:2006 [2]. The material is an automotive diesel fuel containing a volume fraction of approximately 7 % biodiesel that is based on rapeseed oil fatty acid methyl ester with the addition of 1 g/kg antioxidant (butylhydroxytoluene). It was provided by a producer in Germany. The material was filled in amber glass ampoules. Between unit-homogeneity was quantified and stability during dispatch and storage were assessed in accordance with ISO Guide 35:2006 [ ]. The minimum sample intake is the required sample volume stipulated in the respective documentary standard. The material was characterised by an interlaboratory comparison of laboratories of demonstrated competence and adhering to ISO/IEC 17025:2005 [ ]. Technically invalid results were removed but no outlier was eliminated on statistical grounds only. Uncertainties of the certified values were calculated in accordance with the Guide to the Expression of Uncertainty in Measurement (GUM) [ ] and include uncertainties related to possible inhomogeneity, instability and characterisation. The material is intended for quality control and assessment of method performance. As with any reference material, it can be used for establishing control charts or validation studies. The certified reference material (CRM) is available in amber glass ampoules containing 27 mL of automotive diesel fuel material with a volume fraction of 7 % biodiesel closed under argon atmosphere.JRC.F.6-Reference Material

Gas Liquid Chromatographic Determination of Milk Fat and Cocoa Butter Equivalents in Milk Chocolate: Interlaboratory Study

A collaborative trial with 12 laboratories was conducted to validate an analytical approach comprising method procedures for the determination of milk fat and the detection and the quantification of CBEs in milk chocolate. CBE admixtures were detected down to a level of 0.5 g CBE/100 g milk chocolate. The applied quantification model performed well at the statutory limit of 5% CBE addition to milk chocolate with HorRAT values ranging from 0.8 to 1.5. The RSDr for quantification of CBEs in analyses of chocolate fat solutions ranged from 2.2 to 3.8% and in analyses of real chocolate samples, from 4.1 to 4.7%.JRC.D.8-Food safety and qualit

Determination of Nine Intense Sweeteners in Foodstuffs by High Performance Liquid Chromatography and Evaporative Light Scattering Detection: Interlaboratory Study

An interlaboratory trial was conducted to validate an analytical method based on high performance liquid chromatography analysis with evaporative light scattering detection for the simultaneous determination of nine intense sweeteners, i.e., acesulfame-K, alitame, aspartame, cyclamic acid, dulcin, neotame, neohesperidine dihydrochalcone, saccharin and sucralose in carbonated and non-carbonated soft drinks and canned or bottled fruits. Seven laboratories participated in the validation study. High comparability of results obtained by individual testing laboratories was ensured by RSDR values <10 % for the majority of results. Moreover, HorRAT values of less than 1.1 suggested for all sweeteners and matrices tested good performance of the method.JRC.D.8-Food safety and qualit

Validated Method: Method Description for the Quantification of Cocoa Butter Equivalents in Cocoa Butter and Plain Chocolate.

Abstract not availableJRC.D-Institute for Reference Materials and Measurements (Geel

Validation of a Method for the Detection of Cocoa Butter Equivalents in Cocoa Butter and Plain Chocolate. Report on the Validation Study.

Abstract not availableJRC.D-Institute for Reference Materials and Measurements (Geel

Validated Method: Method Description for the Detection of Cocoa Butter Equivalents in Cocoa Butter and Plain Chocolate.

Abstract not availableJRC.D-Institute for Reference Materials and Measurements (Geel

Analysis of Sweeteners in Foodstuff

An analytical approach for the simultaneous determination of nine intense sweeteners in various foodstuffs is presented. By performing a single analysis using high performance liquid chromatography with evaporative light scattering detection acesulfame-K, alitame, aspartame, cyclamic acid, dulcin, neotame, neohesperidine dihydrochalcone, saccharin and sucralose can be determined. The elaborated approach was collaboratively tested, thus proving its validity to be used as an important measure to assess compliance with labelling provisions.JRC.D.8-Food safety and qualit