An analytical approach for the simultaneous determination of nine intense sweeteners in various foodstuffs is presented. By performing a single analysis using high performance liquid chromatography with evaporative light scattering detection acesulfame-K, alitame, aspartame, cyclamic acid, dulcin, neotame, neohesperidine dihydrochalcone, saccharin and sucralose can be determined. The elaborated approach was collaboratively tested, thus proving its validity to be used as an important measure to assess compliance with labelling provisions.JRC.D.8-Food safety and qualit