Determination of Nine Intense Sweeteners in Foodstuffs by High Performance Liquid Chromatography and Evaporative Light Scattering Detection: Interlaboratory Study

Abstract

An interlaboratory trial was conducted to validate an analytical method based on high performance liquid chromatography analysis with evaporative light scattering detection for the simultaneous determination of nine intense sweeteners, i.e., acesulfame-K, alitame, aspartame, cyclamic acid, dulcin, neotame, neohesperidine dihydrochalcone, saccharin and sucralose in carbonated and non-carbonated soft drinks and canned or bottled fruits. Seven laboratories participated in the validation study. High comparability of results obtained by individual testing laboratories was ensured by RSDR values <10 % for the majority of results. Moreover, HorRAT values of less than 1.1 suggested for all sweeteners and matrices tested good performance of the method.JRC.D.8-Food safety and qualit