58 research outputs found
Absence of Ca2+-Induced Mitochondrial Permeability Transition but Presence of Bongkrekate-Sensitive Nucleotide Exchange in C. crangon and P. serratus
Mitochondria from the embryos of brine shrimp (Artemia franciscana) do not undergo Ca2+-induced permeability transition in the presence of a profound Ca2+ uptake capacity. Furthermore, this crustacean is the only organism known to exhibit bongkrekate-insensitive mitochondrial adenine nucleotide exchange, prompting the conjecture that refractoriness to bongkrekate and absence of Ca2+-induced permeability transition are somehow related phenomena. Here we report that mitochondria isolated from two other crustaceans, brown shrimp (Crangon crangon) and common prawn (Palaemon serratus) exhibited bongkrekate-sensitive mitochondrial adenine nucleotide transport, but lacked a Ca2+-induced permeability transition. Ca2+ uptake capacity was robust in the absence of adenine nucleotides in both crustaceans, unaffected by either bongkrekate or cyclosporin A. Transmission electron microscopy images of Ca2+-loaded mitochondria showed needle-like formations of electron-dense material strikingly similar to those observed in mitochondria from the hepatopancreas of blue crab (Callinectes sapidus) and the embryos of Artemia franciscana. Alignment analysis of the partial coding sequences of the adenine nucleotide translocase (ANT) expressed in Crangon crangon and Palaemon serratus versus the complete sequence expressed in Artemia franciscana reappraised the possibility of the 208-214 amino acid region for conferring sensitivity to bongkrekate. However, our findings suggest that the ability to undergo Ca2+-induced mitochondrial permeability transition and the sensitivity of adenine nucleotide translocase to bongkrekate are not necessarily related phenomena
Healable Cellulose Iontronic Hydrogel Stickers for Sustainable Electronics on Paper
The authors acknowledge the support from FCT - Portuguese Foundation for Science and Technology through the Ph.D. scholarships SFRH/BD/126409/2016 (I.C.) and SFRH/BD/122286/2016 (J.M.). The authors would like to acknowledge the European Commission under project NewFun (ERC-StG-2014, GA 640598) and project SYNERGY (H2020-WIDESPREAD-2020-5, CSA, proposal no 952169). This work was also supported by the FEDER funds through the COMPETE 2020 Program and the National Funds through the FCT - Portuguese Foundation for Science and Technology under the Project No. POCI-01-0145-FEDER-007688, reference UID/CTM/50025, project CHIHC, reference PTDC/NAN-MAT/32558/2017. The authors would also like to thank their colleagues Daniela Gomes and Ana Pimentel from CENIMAT/i3N for the SEM and DSC-TGA measurements, respectively.Novel nature-based engineered functional materials combined with sustainable and economically efficient processes are among the great challenges for the future of mankind. In this context, this work presents a new generation of versatile flexible and highly conformable regenerated cellulose hydrogel electrolytes with high ionic conductivity and self-healing ability, capable of being (re)used in electrical and electrochemical devices. They can be provided in the form of stickers and easily applied as gate dielectric onto flexible indium–gallium–zinc oxide transistors, decreasing the manufacturing complexity. Flexible and low-voltage (<2.5 V) circuits can be handwritten on-demand on paper transistors for patterning of conductive/resistive lines. This user-friendly and simplified manufacturing approach holds potential for fast production of low-cost, portable, disposable/recyclable, and low-power ion-controlled electronics on paper, making it attractive for application in sensors and concepts such as the “Internet-on-Things.”.publishersversionpublishe
Second Order Perturbations in the Randall-Sundrum Infinite Brane-World Model
We discuss the non-linear gravitational interactions in the Randall-Sundrum
single brane model. If we naively write down the 4-dimensional effective action
integrating over the fifth dimension with the aid of the decomposition with
respect to eigen modes of 4-dimensional d'Alembertian, the Kaluza-Klein mode
coupling seems to be ill-defined. We carefully analyze second order
perturbations of the gravitational field induced on the 3-brane under the
assumption of the static and axial-symmetric 5-dimensional metric. It is shown
that there remains no pathological feature in the Kaluza-Klein mode coupling
after the summation over all different mass modes. Furthermore, the leading
Kaluza-Klein corrections are shown to be sufficiently suppressed in comparison
with the leading order term which is obtained by the zero mode truncation. We
confirm that the 4-dimensional Einstein gravity is approximately recovered on
the 3-brane up to second order perturbations.Comment: 15 pages, 2 figures, comment and reference added, typos correcte
Mantle margin morphogenesis in Nodipecten nodosus (Mollusca: Bivalvia): new insights into the development and the roles of bivalve pallial folds
Induction of resistance to ectromelia virus infection by corynebacterium parvum in murine peritoneal macrophages.
Abortive ectromelia virus infection in peritoneal macrophages activated by corynebacterium parvum.
In situ fixation of cultured mouse peritoneal exudate cells: comparison of fixation methods.
Development of protective immunity against bacterial and viral infections in tumor-bearing mice coincident with suppression of tumor immunity.
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