Freeform Deposition Method for Coolant Channel Closeout

A method is provided for fabricating a coolant channel closeout jacket on a structure having coolant channels formed in an outer surface thereof. A line of tangency relative to the outer surface is defined for each point on the outer surface. Linear rows of a metal feedstock are directed towards and deposited on the outer surface of the structure as a beam of weld energy is directed to the metal feedstock so-deposited. A first angle between the metal feedstock so-directed and the line of tangency is maintained in a range of 20-90.degree.. The beam is directed towards a portion of the linear rows such that less than 30% of the cross-sectional area of the beam impinges on a currently-deposited one of the linear rows. A second angle between the beam and the line of tangency is maintained in a range of 5-65 degrees

Process Optimisation of Steam Explosion Parameters on Multiple Lignocellulosic Biomass using Taguchi Method:A Critical Appraisal

Xylitol is a low calorie sweetener that can be produced through a bioconversion approach from lignocellulosic biomass that requires pretreatment prior to the bioconversion of xylose to xylitol. Steam explosion (SE) is an industrially scalable pretreatment (PT) process with the potential to liberate xylose monomers, however SE-PT has not been optimised for xylose release from multiple feedstock. The effect of pressure, substrate weight, phosphoric acid loading concentration and residence time on four feedstock (wheat straw (WS), corn stover (CS), Miscanthus (M), and willow (W)) for xylose release and minimal fermentation inhibitor productions (furfural and 5-hydroxymethylfurfural (HMF)) was investigated using the Taguchi methodology for design of experiment (DoE) with variation at four levels (44). An L16 orthogonal array design was utilised and all factors indicated influence on xylose release and inhibitor formation and the resulting xylose rich hydrolysate assessed for bioconversion to xylitol.. The L16 DoE gave hydrolysates containing 75-95% of xylose content in the original biomass, whilst retaining cellulose and lignin components in the fibre. The level of inhibitors were within boundary limits to enable microbial fermentation of the hydrolysates to xylitol. Fine tuning of the overall evaluation criteria (OEC) model imbibing 1.5 kg feedstock in 1.2% w/v orthophosphoric acid, 12 bar(g) and 6 minutes residence time resulted in 90% xylose recovery and production of >1000 L of wheat straw hydrolysate for bioconversion to xylitol. The advantages and limitations of the Taguchi OEC model and further improvements to this process are discussed in a biorefining contextpublishersversionPeer reviewe

Afadin orients cell division to position the tubule lumen in developing renal tubules

In many types of tubules, continuity of the lumen is paramount to tubular function, yet how tubules generate lumen continuity in vivo is not known. We recently found the F-actin binding protein Afadin is required for lumen continuity in developing renal tubules, though its mechanism of action remains unknown. Here we demonstrate Afadin is required for lumen continuity by orienting the mitotic spindle during cell division. Using an in vitro 3D cyst model, we find Afadin localizes to the cell cortex adjacent to the spindle poles and orients the mitotic spindle. In tubules, cell division may be oriented relative to two axes, longitudinal and apical-basal. Unexpectedly, in vivo examination of early stage developing nephron tubules reveals cell division is not oriented in the longitudinal (or planar polarized) axis. However, cell division is oriented perpendicular to the apical-basal axis. Absence of Afadin in vivo leads to misorientation of apical-basal cell division in nephron tubules. Together these results support a model whereby Afadin determines lumen placement by directing apical-basal spindle orientation, which generates a continuous lumen and normal tubule morphogenesis

Surficial fractures in the Navajo sandstone, south-western USA: the roles of thermal cycles, rainstorms, granular disintegration, and iterative cracking

Deep (\u3e 5 m) sheeting fractures in the Navajo sandstone are evident at numerous sites in southern Utah and derive from tectonic stresses. Strong diurnal thermal cycles are, however, the likely triggers for shallow (\u3c 0.3 m) sheeting fractures. Data from subsurface thermal sensors reveal that large temperature differences between sensors at 2 and 15 cm depth on clear summer afternoons are as great as those that trigger sheeting fractures in exposed California granite. Extensive polygonal patterns in the Navajo sandstone are composed of surface-perpendicular fractures and were produced by contractile stresses. Numerous studies have shown that porewater diminishes the tensile strength of sandstone. Based on our thermal records, we propose that cooling during monsoonal rainstorms triggers polygonal fracturing of temporarily weakened rock. On steep outcrops, polygonal patterns are rectilinear and orthogonal, with T-vertices. Lower-angle slopes host hexagonal patterns (defined by the dominance of Y-vertices). Intermediate patterns with rectangles and hexagons of similar scale are common. We posit that outcropping fractures are advancing downward by iterative steps, and that hexagons on sandstone surfaces (like prismatic columns of basalt) have evolved from ancestral orthogonal polygons of similar scale. In lava flows, fractures elongate intermittently as they follow a steep thermal gradient (the source of stress) as it rapidly moves through the rock mass. In our model, a steep, surficial thermal gradient descends through unfractured sandstone, but at the slow pace of granular disintegration. Through time, as the friable rock on stable slopes erodes, iterative cracking advances into new space. Hexagonal patterns form as new fractures, imperfectly guided by the older ones, propagate in new directions, and vertices drift into a configuration that minimizes the ratio of fracture length to polygon area

Par3 integrates Tiam1 and phosphatidylinositol 3-kinase signaling to change apical membrane identity

Pathogens can alter epithelial polarity by recruiting polarity proteins to the apical membrane, but how a change in protein localization is linked to polarity disruption is not clear. In this study, we used chemically induced dimerization to rapidly relocalize proteins from the cytosol to the apical surface. We demonstrate that forced apical localization of Par3, which is normally restricted to tight junctions, is sufficient to alter apical membrane identity through its interactions with phosphatidylinositol 3-kinase (PI3K) and the Rac1 guanine nucleotide exchange factor Tiam1. We further show that PI3K activity is required upstream of Rac1, and that simultaneously targeting PI3K and Tiam1 to the apical membrane has a synergistic effect on membrane remodeling. Thus, Par3 coordinates the action of PI3K and Tiam1 to define membrane identity, revealing a signaling mechanism that can be exploited by human mucosal pathogens

Syntaphilin Ubiquitination Regulates Mitochondrial Dynamics and Tumor Cell Movements.

Syntaphilin (SNPH) inhibits the movement of mitochondria in tumor cells, preventing their accumulation at the cortical cytoskeleton and limiting the bioenergetics of cell motility and invasion. Although this may suppress metastasis, the regulation of the SNPH pathway is not well understood. Using a global proteomics screen, we show that SNPH associates with multiple regulators of ubiquitin-dependent responses and is ubiquitinated by the E3 ligase CHIP (or STUB1) on Lys111 and Lys153 in the microtubule-binding domain. SNPH ubiquitination did not result in protein degradation, but instead anchored SNPH on tubulin to inhibit mitochondrial motility and cycles of organelle fusion and fission, that is dynamics. Expression of ubiquitination-defective SNPH mutant Lys111!Arg or Lys153!Arg increased the speed and distance traveled by mitochondria, repositioned mitochondria to the cortical cytoskeleton, and supported heightened tumor chemotaxis, invasion, and metastasis in vivo. Interference with SNPH ubiquitination activated mitochondrial dynamics, resulting in increased recruitment of the fission regulator dynamin-related protein-1 (Drp1) to mitochondria and Drp1-dependent tumor cell motility. These data uncover nondegradative ubiquitination of SNPH as a key regulator of mitochondrial trafficking and tumor cell motility and invasion. In this way, SNPH may function as a unique, ubiquitination-regulated suppressor of metastasis

Supersymmetric black rings and three-charge supertubes

We present supergravity solutions for 1/8-supersymmetric black supertubes with three charges and three dipoles. Their reduction to five dimensions yields supersymmetric black rings with regular horizons and two independent angular momenta. The general solution contains seven independent parameters and provides the first example of non-uniqueness of supersymmetric black holes. In ten dimensions, the solutions can be realized as D1-D5-P black supertubes. We also present a worldvolume construction of a supertube that exhibits three dipoles explicitly. This description allows an arbitrary cross-section but captures only one of the angular momenta.Comment: 59 pages, 6 figures; v2: minor correction