Uso de marcadores moleculares para a solução de questões de identidade de cultivares de videira.

O objetivo deste trabalho é demonstrar como a análise genético-molecular pode contribuir para resolver questões de identidade

Uso de marcadores moleculares SSR na identificação de cultivares de videira.

A ocorrência de sinonímias e/ou homonímias, muitas vezes consequência da mistura de material propagativo de cultivares diferentes, é uma barreira para o setor vitivinícola. As caracterizações morfológica e molecular têm sido usadas pelo Laboratório do Banco Ativo de Germoplasma de Uva para a identificação correta de cultivares. O objetivo deste trabalho foi demonstrar como a análise genético-molecular pode contribuir para resolver questões de identidade

Seleção de marcadores moleculares para caracterização genética da cultivar de videira Moscato Branco.

Este trabalho visa caracterizar um grupo de marcadores moleculares para estabelecer as diferenças entre a 'Moscato Branco' e outras cultivares moscatel cultivadas na Serra Gaúcha

Uso de marcadores moleculares microssatélites para caracterização de uva 'Moscato Branco' cultivada na serra gaúcha.

Na Serra Gaúcha a uva 'Moscato Branco' é cultivada com sucesso desde 1930, com fortes evidências indicando sua produção exclusiva ao Brasil. Para contribuir com a caracterização da cultivar utilizou-se um grupo de 17 marcadores microssatélites. O DNA foi extraído de sete amostras de folhas jovens de 'Moscato Branco', coletadas em áreas de produtores, e nove amostras de outras uvas tipo moscatel cultivadas na região, amplificado em reação de PCR e separado em gel de poliacrilamida 6%, corado com prata. Os marcadores foram caracterizados com o uso do programa GDA

Diversidade genética do Banco Ativo de Germoplasma de Pera avaliada com o uso de marcadores moleculares SSR.

O objetivo deste trabalho foi caracterizar e avaliar a diversidade genética entre os acessos de pereira existentes no Banco Ativo de Germoplasma de Pera (BAG-Pera) com o uso de marcadores moleculares SSR (Simple Sequence Repeats), visando organizar os recursos genéticos da espécie e apoiar as atividades de melhoramento genético

Avaliação da diversidade genética do banco ativo de germoplasma de pera (BAG-Pera) utilizando marcadores moleculares SSR.

O objetivo deste trabalho foi caracterizar e avaliar a diversidade genética entre os acessos de pereira existentes no BAG-Pera utilizando marcadores moleculares SSR, visando organizar os recursos genéticos e apoiar as atividades de melhoramento genético

ABA triblock copolymers: from controlled synthesis to controlled function

The ABA amphiphilic block copolymers, poly(hydroxyethyl methacrylate-hlock-methylphenylsilane-block-hydroxyethyl methacrylate) (PHEMA-PMPS-PHEMA) and poly[oligo(ethylene glycol) methyl ether methacrylate-block-methylphenylsilane-block-oligo(ethylene glycol). methyl ether methacrylate] (POEGMA-PMPS-POEGMA) were successfully synthesised via atom transfer radical polymerisation (ATRP). Macroinitiators suitable for the ATRP of oligo(ethylene glycol) methyl ether methacrylate and 2-hydroxyethyl methacrylate were synthesised from the condensation reaction of alpha,omega-dihalopolymethylphenylsilane and 2'-hydroxyethyl 2-bromo-2-methylpropanoate. The copolymers were characterised using H-1 NMR and C-13 NMR spectroscopy and molecular weight characteristics were determined using size exclusion chromatography and H-1 NMR. The aggregation behaviour of some of the copolymers in water was studied using transmission and scanning electron microscopy and dynamic light scattering. These revealed the prevalent aggregate species to be micelles. Larger aggregates of 300-1000 nm diameter were also observed. The UV induced degradation of the aggregates was studied by UV-Vis spectroscopy. The thermal behaviour of selected copolymers was studied by differential scanning calorimetry and microphase separation of the two components was demonstrated

Caracterização da cultivar de videira moscato branco.

A videira―Moscato Branco‖ é cultivada na Serra Gaúcha, especialmente no município de Farroupilha-RS,desde os anos 1930. Análises moleculares já realizadas indicam que a cultivar difere de acessos do tipo moscatel mantidos no Banco Ativo de Germoplasma de Uva e também de outras cultivares de uva moscatel cultivadas na região. Onze acessos de ̳Moscato Branco?, coletados por sua potencialidade em apresentar diferenças clonais, estão sendo avaliados em nível agronômico, físico -químico e fenológico, juntamente com acessos de ̳Moscato Branco R2?(seis acessos), ̳Malvasia Bianca?, ̳Malvasia Aromática?, ̳Moscato Embrapa?, ̳BRS Lorena?, ̳Walsh Riesling?e ̳Pignoleto?, mantidos no Banco Ativo de Germoplasma de Uva. Os dados foram coletados no campo experimental na Embrapa Uva e Vinho, por quatro safras (2012/13 a 2015/16),com o objetivo de identificar clones de ̳Moscato Branco?, que apresentem cachos maissoltos, e também de caracterizar esta cultivar em relação a outras uvas cultivadas na região

In vitro effects of lapachol and β-lapachone against Leishmania amazonensis

Leishmaniasis is a neglected disease that affects millions of people worldwide, and special attention should be given to treatment because the available drugs have limitations, which can lead to low therapeutic adherence and parasitic resistance. This study evaluated the activity of the bioactive naphthoquinones, lapachol and β-lapachone, against Leishmania amazonensis. The cell alterations were evaluated in vitro on promastigote and amastigote forms. The lethal dose (LD50) at 24, 48, and 72 h on the promastigote's forms using lapachol was 75.60, 72.82, and 58.85 μg/mL and for β-lapachone was 0.65, 1.24, and 0.71 μg/mL, respectively. The naphthoquinones significantly inhibited the survival rate of L. amazonensis amastigotes at 83.11, 57.59, and 34.95% for lapachol (82.28, 41.14, and 20.57 µg/mL), and 78.49, 83.25, and 80.22% for β-lapachone (3.26, 1.63, and 0.815 µg/mL). The compounds on the promastigote's forms led to the loss of mitochondrial membrane potential, induced changes in the integrity of the membrane, caused damage to cells suggestive of the apoptotic process, and showed inhibition of tumor necrosis factor (TNF)-α and interleukin (IL)-6 production. The results showed that these naphthoquinones are promising candidates for research on new drugs with anti-Leishmania activity derived from natural products

The effect of pond dyes on oviposition and survival in wild UK Culex mosquitoes

British Culex pipiens complex [Culex pipiens sensu lato) mosquito distribution, abundance, and potential for disease transmission are intimately linked to their environment. Pond and lake dyes that block light to restrict algal photosynthesis are a relatively new product assumed to be an environmentally friendly since they are based on food dyes. Their use in urban garden ponds raises questions linked to mosquito oviposition, since coloured water can be an attractant. Culex (mostly pipiens) is commonly found in UK gardens and is a potential vector of viruses including the West Nile Virus (WNV). Any factors that significantly change the distribution and population of Cx pipiens could impact future risks of disease transmission. A gravid trap was used to catch female Cx pipiens mosquitoes for use in oviposition choice tests in laboratory and semi-field conditions. Two types of pond dye, blue and shadow (which looks slightly red), were tested for their impact on oviposition and survival of wild caught Cx pipiens. There were no significant differences in the number of egg batches laid when gravid mosquitoes were given a choice between either blue dye and clear water or shadow dye and clear water indicating that these dyes are not attractants. Larvae hatched from egg batches laid by wild-caught gravid females were used to measure survival to adulthood with or without dye, , in a habitat controlled to prevent further colonisation. The experiment was run twice, once in the summer and again in the autumn, whereas the dyes had no impact on emergence in the summer, there were highly significant reductions in emergence of adults in both dye treated habitats in the autumn. Containers with or without shadow dye were placed outside to colonise naturally and were sampled weekly for larvae and pupae over a 6 month period through summer and autumn. There was a significant negative effect of shadow dye on pupal abundance in a three week period over the summer, but otherwise there was no effect. It is likely that population abundance and food was a more powerful factor for mosquito survival than the dye