279 research outputs found
Alterations in microbial community composition with increasing fCO2: a mesocosm study in the eastern Baltic Sea
Ocean acidification resulting from the uptake of anthropogenic carbon
dioxide (CO<sub>2</sub>) by the ocean is considered a major threat to marine
ecosystems. Here we examined the effects of ocean acidification on microbial
community dynamics in the eastern Baltic Sea during the summer of 2012 when
inorganic nitrogen and phosphorus were strongly depleted. Large-volume in
situ mesocosms were employed to mimic present, future and far future
CO<sub>2</sub> scenarios. All six groups of phytoplankton enumerated by flow
cytometry ( <  20 µm cell diameter) showed distinct trends in
net growth and abundance with CO<sub>2</sub> enrichment. The picoeukaryotic
phytoplankton groups Pico-I and Pico-II displayed enhanced abundances, whilst
Pico-III, <i>Synechococcus</i> and the nanoeukaryotic phytoplankton groups
were negatively affected by elevated fugacity of CO<sub>2</sub>
(<i>f</i>CO<sub>2</sub>). Specifically, the numerically dominant eukaryote, Pico-I,
demonstrated increases in gross growth rate with increasing <i>f</i>CO<sub>2</sub>
sufficient to double its abundance. The dynamics of the prokaryote community
closely followed trends in total algal biomass despite differential effects
of <i>f</i>CO<sub>2</sub> on algal groups. Similarly, viral abundances corresponded
to prokaryotic host population dynamics. Viral lysis and grazing were both
important in controlling microbial abundances. Overall our results point to a
shift, with increasing <i>f</i>CO<sub>2</sub>, towards a more regenerative system
with production dominated by small picoeukaryotic phytoplankton.</p
Effect of CO2 enrichment on bacterial metabolism in an Arctic fjord
he anthropogenic increase of carbon dioxide (CO2) alters the seawater carbonate chemistry, with a decline of pH and an increase in the partial pressure of CO2 (pCO2). Although bacteria play a major role in carbon cycling, little is known about the impact of rising pCO2 on bacterial carbon metabolism, especially for natural bacterial communities. In this study, we investigated the effect of rising pCO2 on bacterial production (BP), bacterial respiration (BR) and bacterial carbon metabolism during a mesocosm experiment performed in Kongsfjorden (Svalbard) in 2010. Nine mesocosms with pCO2 levels ranging from ca. 180 to 1400 ÎĽatm were deployed in the fjord and monitored for 30 days. Generally BP gradually decreased in all mesocosms in an initial phase, showed a large (3.6-fold average) but temporary increase on day 10, and increased slightly after inorganic nutrient addition. Over the wide range of pCO2 investigated, the patterns in BP and growth rate of bulk and free-living communities were generally similar over time. However, BP of the bulk community significantly decreased with increasing pCO2 after nutrient addition (day 14). In addition, increasing pCO2 enhanced the leucine to thymidine (Leu : TdR) ratio at the end of experiment, suggesting that pCO2 may alter the growth balance of bacteria. Stepwise multiple regression analysis suggests that multiple factors, including pCO2, explained the changes of BP, growth rate and Leu : TdR ratio at the end of the experiment. In contrast to BP, no clear trend and effect of changes of pCO2 was observed for BR, bacterial carbon demand and bacterial growth efficiency. Overall, the results suggest that changes in pCO2 potentially influence bacterial production, growth rate and growth balance rather than the conversion of dissolved organic matter into CO2
Arctic microbial community dynamics influenced by elevated CO2 levels
The Arctic Ocean ecosystem is particularly vulnerable to ocean acidification (OA) related alterations due to the relatively high CO2 solubility and low carbonate saturation states of its cold surface waters. Thus far, however, there is only little known about the consequences of OA on the base of the food web. In a mesocosm CO2-enrichment experiment (overall CO2 levels ranged from ~ 180 to 1100 ÎĽatm) in Kongsfjorden off Svalbard, we studied the consequences of OA on a natural pelagic microbial community. OA distinctly affected the composition and growth of the Arctic phytoplankton community, i.e. the picoeukaryotic photoautotrophs and to a lesser extent the nanophytoplankton thrived. A shift towards the smallest phytoplankton as a result of OA will have direct consequences for the structure and functioning of the pelagic food web and thus for the biogeochemical cycles. Besides being grazed, the dominant pico- and nanophytoplankton groups were found prone to viral lysis, thereby shunting the carbon accumulation in living organisms into the dissolved pools of organic carbon and subsequently affecting the efficiency of the biological pump in these Arctic waters
Marine viruses discovered via metagenomics shed light on viral strategies throughout the oceans
Marine viruses are key drivers of host diversity, population dynamics and biogeochemical cycling and contribute to the daily flux of billions of tons of organic matter. Despite recent advancements in metagenomics, much of their biodiversity remains uncharacterized. Here we report a data set of 27,346 marine virome contigs that includes 44 complete genomes. These outnumber all currently known phage genomes in marine habitats and include members of previously uncharacterized lineages. We designed a new method for host prediction based on co-occurrence associations that reveals these viruses infect dominant members of the marine microbiome such as Prochlorococcus and Pelagibacter. A negative association between host abundance and the virus-to-host ratio supports the recently proposed Piggyback-the-Winner model of reduced phage lysis at higher host densities. An analysis of the abundance patterns of viruses throughout the oceans revealed how marine viral communities adapt to various seasonal, temperature and photic regimes according to targeted hosts and the diversity of auxiliary metabolic genes.CAPESCNPqFAPERJCiencia sem fronteiras programUniv Fed Rio de Janeiro, IB, BR-21944970 Rio de Janeiro, BrazilRadboud Univ Nijmegen, Radboud Inst Mol Life Sci, CMBI, Med Ctr, NL-6500 HB Nijmegen, NetherlandsUniv Utrecht, Theoret Biol & Bioinformat, NL-3584 CH Utrecht, NetherlandsSan Diego State Univ, Dept Biol, San Diego, CA 92182 USAUniv Fed Sao Paulo UNIFESP, Dept Ciencias Mar, BR-11070100 Baixada Santista, BrazilNIOZ Royal Netherlands Inst Sea Res, Dept Marine Microbiol & Biogeochem, POB 59, NL-1790 AB Den Burg, NetherlandsUniv Utrecht, POB 59, NL-1790 AB Den Burg, NetherlandsUniv Amsterdam, Dept Aquat Microbiol, IBED, NL-1090 GE Amsterdam, NetherlandsUniv Fed Rio de Janeiro, COPPE, SAGE, BR-21941950 Rio de Janeiro, BrazilUniv Fed Sao Paulo UNIFESP, Dept Ciencias Mar, BR-11070100 Baixada Santista, BrazilCAPESCNPqFAPERJCiencia sem fronteiras program: 864.14.004Web of Scienc
Calculation of the properties of the rotational bands of Gd
We reexamine the long-standing problem of the microscopic derivation of a
particle-core coupling model. We base our research on the Klein-Kerman
approach, as amended by D\"onau and Frauendorf. We describe the formalism to
calculate energy spectra and transition strengths in some detail. We apply our
formalism to the rotational nuclei Gd, where recent experimental
data requires an explanation. We find no clear evidence of a need for Coriolis
attenuation.Comment: 27 pages, 13 uuencoded postscript figures. Uses epsf.st
Comparison of techniques for computing shell-model effective operators
Different techniques for calculating effective operators within the framework
of the shell model using the same effective interaction and the same excitation
spaces are presented. Starting with the large-basis no-core approach, we
compare the time-honored perturbation-expansion approach and a model-space
truncation approach. Results for the electric quadrupole and magnetic dipole
operators are presented for Li. The convergence trends and dependence of
the effective operators on differing excitation spaces and Pauli Q-operators is
studied. In addition, the dependence of the electric-quadrupole effective
charge on the harmonic-oscillator frequency and the mass number, for A=5,6, is
investigated in the model-space truncation approach.Comment: 18 pages. REVTEX. 4 PostScript figure
Reaction rates for Neutron Capture Reactions to C-, N- and O-isotopes to the neutron rich side of stability
The reaction rates of neutron capture reactions on light nuclei are important
for reliably simulating nucleosynthesis in a variety of stellar scenarios.
Neutron capture reaction rates on neutron-rich C-, N-, and O-isotopes are
calculated in the framework of a hybrid compound and direct capture model. The
results are tabulated and compared with the results of previous calculations as
well as with experimental results.Comment: 33 pages (uses revtex) and 9 postscript figures, accepted for
publication in Phys. Rev.
Carbon Nitride thin films prepared by a capacitively coupled RF plasma jet
Abstract Carbon nitride thin films have been downstream deposited from a nitrogen plasma beam sustained by a capacitively coupled discharge generated between a RF powered carbon electrode and a grounded carbon nozzle. The spectral emission of the plasma jet strongly exhibits the CN radical emission indicating that the deposition takes place via a mechanism involving the CN radical. The deposition process is enhanced by DC biasing the powered electrode. The films have been investigated by X-ray diffraction, infrared absorption spectroscopy and X-ray photoelectron spectroscopy. The results show that the films are amorphous and contain in a large extent carbon nitrogen bonds
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