Palaeogenomics of Upper Palaeolithic to Neolithic European hunter-gatherers

Modern humans have populated Europe for more than 45,000 years1,2. Our knowledge of the genetic relatedness and structure of ancient hunter-gatherers is however limited, owing to the scarceness and poor molecular preservation of human remains from that period3. Here we analyse 356 ancient hunter-gatherer genomes, including new genomic data for 116 individuals from 14 countries in western and central Eurasia, spanning between 35,000 and 5,000 years ago. We identify a genetic ancestry profile in individuals associated with Upper Palaeolithic Gravettian assemblages from western Europe that is distinct from contemporaneous groups related to this archaeological culture in central and southern Europe4, but resembles that of preceding individuals associated with the Aurignacian culture. This ancestry profile survived during the Last Glacial Maximum (25,000 to 19,000 years ago) in human populations from southwestern Europe associated with the Solutrean culture, and with the following Magdalenian culture that re-expanded northeastward after the Last Glacial Maximum. Conversely, we reveal a genetic turnover in southern Europe suggesting a local replacement of human groups around the time of the Last Glacial Maximum, accompanied by a north-to-south dispersal of populations associated with the Epigravettian culture. From at least 14,000 years ago, an ancestry related to this culture spread from the south across the rest of Europe, largely replacing the Magdalenian-associated gene pool. After a period of limited admixture that spanned the beginning of the Mesolithic, we find genetic interactions between western and eastern European hunter-gatherers, who were also characterized by marked differences in phenotypically relevant variants

Palaeogenomics of Upper Palaeolithic to Neolithic European hunter-gatherers

Modern humans have populated Europe for more than 45,000 years(1,2). Our knowledge of the genetic relatedness and structure of ancient hunter-gatherers is however limited, owing to the scarceness and poor molecular preservation of human remains from that period(3). Here we analyse 356 ancient hunter-gatherer genomes, including new genomic data for 116 individuals from 14 countries in western and central Eurasia, spanning between 35,000 and 5,000 years ago. We identify a genetic ancestry profile in individuals associated with Upper Palaeolithic Gravettian assemblages from western Europe that is distinct from contemporaneous groups related to this archaeological culture in central and southern Europe(4), but resembles that of preceding individuals associated with the Aurignacian culture. This ancestry profile survived during the Last Glacial Maximum (25,000 to 19,000 years ago) in human populations from southwestern Europe associated with the Solutrean culture, and with the following Magdalenian culture that re-expanded northeastward after the Last Glacial Maximum. Conversely, we reveal a genetic turnover in southern Europe suggesting a local replacement of human groups around the time of the Last Glacial Maximum, accompanied by a north-to-south dispersal of populations associated with the Epigravettian culture. From at least 14,000 years ago, an ancestry related to this culture spread from the south across the rest of Europe, largely replacing the Magdalenian-associated gene pool. After a period of limited admixture that spanned the beginning of the Mesolithic, we find genetic interactions between western and eastern European hunter-gatherers, who were also characterized by marked differences in phenotypically relevant variants.Molecular Technology and Informatics for Personalised Medicine and Healt

Palaeogenomics of Upper Palaeolithic to Neolithic European hunter-gatherers

Modern humans have populated Europe for more than 45,000 years. Our knowledge of the genetic relatedness and structure of ancient hunter-gatherers is however limited, owing to the scarceness and poor molecular preservation of human remains from that period. Here we analyse 356 ancient hunter-gatherer genomes, including new genomic data for 116 individuals from 14 countries in western and central Eurasia, spanning between 35,000 and 5,000 years ago. We identify a genetic ancestry profile in individuals associated with Upper Palaeolithic Gravettian assemblages from western Europe that is distinct from contemporaneous groups related to this archaeological culture in central and southern Europe, but resembles that of preceding individuals associated with the Aurignacian culture. This ancestry profile survived during the Last Glacial Maximum (25,000 to 19,000 years ago) in human populations from southwestern Europe associated with the Solutrean culture, and with the following Magdalenian culture that re-expanded northeastward after the Last Glacial Maximum. Conversely, we reveal a genetic turnover in southern Europe suggesting a local replacement of human groups around the time of the Last Glacial Maximum, accompanied by a north-to-south dispersal of populations associated with the Epigravettian culture. From at least 14,000 years ago, an ancestry related to this culture spread from the south across the rest of Europe, largely replacing the Magdalenian-associated gene pool. After a period of limited admixture that spanned the beginning of the Mesolithic, we find genetic interactions between western and eastern European hunter-gatherers, who were also characterized by marked differences in phenotypically relevant variants.Archaeological Heritage Managemen

Out of the Cave and into the Light: Perspectives and Challenges of Radiocarbon Dating an Open-Air Aurignacian Site (Régismont-le-Haut, Mediterranean France)

International audienceThis article presents the methods and results regarding the radiocarbon (AMS) dating of the site of Régismont-le-Haut. This site is one of the very few well-preserved Aurignacian open-air sites in southern France. It is also one of the only Aurignacian sites in France and Europe, more generally, to have conserved evident features, including many in situ fire-related structures. We selected charcoal and bone from all the major occupation sectors excavated so far, as well as from others which were important for understanding site formation. Considering difficulties faced when comparing the lithic industry of a site located in a poorly documented area with those belonging to the Aquitaine basin, where the "classical" definition of the Aurignacian was outlined, dating was conducted to accurately place the site within this technocomplex's internal chronology. In total, 19 charcoals (identified to Pinus sp. or gymnosperm) and 3 bones were submitted for AMS radiocarbon dating, each to one of three laboratories. We implemented a strict charcoal screening protocol for radiometric dating that required genus-identified individual charcoals that were well-associated with the fire-related structures. Despite this careful selection , most of the charcoals and all of the bones contained insufficient carbon for dating. Of the 19 charcoal dates attempted, 5 produced dates, from two loci. All fall in the span between the Middle Aurignacian and the Recent Aurignacian (between around 32,500 and 28,000 uncalibrated 14 C BP). An older subset of the dates (32.5-32 ka BP, ca. 37.5-35.5 ka cal BP) appears to be the most reliable. These results represent the first AMS dates ever performed on an Aurignacian open-air site in the Mediterranean area. The dates are compared to other recently dated southwestern France sites to establish which ones might be useful for future detailed archaeological comparisons. They are also instructive from a methodological point of view, in order to better appreciate the resolution of radiocarbon in this context. The focus of this article is on presenting the dating work, especially the protocol used in the selection of samples, the laboratory methods used for obtaining the dates and the challenges faced at this site. Among these, the divergence of dates between loci contrasted with geomorphological and archaeological evidence, with the latter two strongly pointing to one living floor, and either a single occupation or a few very close in time. This forced a reassessment of the chronological dating evidence. This article serves to show the importance of multi-prong approaches to archaeological dating, comparing and contrasting both contextual evidence and actual dates