Measurement of bone quality in growing male rats using dual energy x-ray absorptiometry and bone ash content : a thesis presented in partial fulfilment of the requirements for the degree of Master of Applied Science in Animal Science at Massey University, Palmerston North, New Zealand

Growing male rats have been considered and used as a model for bone growth and prevention of osteoporosis because of their high bone turnover and demand for calcium. Dual Energy X-ray Absorptiometry (DEXA) is a useful tool for identifying minimal changes in bone mineral density and has recently been adapted for use in small animal models. The objective of this trial was to identify the changes in Bone Mineral Density (BMD) in relation to age and to identify how BMD varies from site to site. Sixty male Sprague-Dawley rats were split into six groups to allow measurements at one, two, three, four, five and six months of age (n=10 per group). At each time point a group of rats was scanned using a QDR4000 DEXA machine from Hologic. Duplicate BMD measurements were obtained for the whole body, spine and both femurs in vivo. The rats were then euthanased and the spine and both femurs were excised for ex vivo DEXA scanning and ashed calcium analysis. BMD increased almost linearly to four months and then formed a plateau. This indicates that from weaning to four months is an especially sensitive time for manipulating bone growth in male rats. There was a significant difference in BMD between groups (P<0.001), which is to be expected in growing rats. There was also a significant difference in BMD within groups (p<0.001), believed to be due to variation at two and five months of age. There was a very strong positive correlation between weight and BMD and age and BMD at all sites, indicating that BMD is a strongly related to both weight and age. All sites were strongly correlated to each other and to the ashed calcium values. The excised femur had a lower BMD value than the in vivo femur, although the two values were strongly correlated. This is believed to be due to differences in positioning and indicates that the two methods cannot be used interchangeably. These results indicate that bone mineral density is the gold standard for following changes in bone growth over time in the growing rat. Alternatively, ashed bone calcium content can be used, but only as a once off endpoint

Optical coherence tomography:age estimation of <i>Calliphora vicina</i> pupae <i>in vivo</i>?

Necrophagous blowfly pupae are valuable contributors to the estimation of post-mortem interval, should an accurate age estimate be obtained. At present, this is reliant on a combination of rearing and destructive methods conducted on preserved samples, including morphological observation and gene expression analyses. This study demonstrates the use of optical coherence tomography (OCT) as a tool for in vivo morphological observation and pupal age estimation. Using a Michelson OCT microscope, alive and preserved four and ten-day old Calliphora vicina pupae were scanned in different orientations. Two and three-dimensional images were created. Morphological characteristics such as the brain, mouthparts and legs were identifiable in both living and preserved samples, with distinct differences noted between the two ages. Absorption of light by the puparium results in a vertical resolution of 1-2 mm, preventing observation of deeper tissues. The use of contrast agents or a longer wavelength laser would improve the images obtainable. At present, the data suggests OCT provides a primary view of external and internal morphology, which can be used to distinguish younger and older pupae for further analysis of age and PMI estimation

Are gauge symmetry transformations observable?

In a recent paper in the British Journal for the Philosophy of Science, Kosso discussed the observational status of continuous symmetries of physics. While we are in broad agreement with his approach, we disagree with his analysis. In the discussion of the status of gauge symmetry, a set of examples offered by ’t Hooft has influenced several philosophers, including Kosso; in all cases the interpretation of the examples is mistaken. In this paper we present our preferred approach to the empirical significance of symmetries, re-analysing the cases of gauge symmetry and general covariance

Europa's Optical Aurora

Auroral emissions provide opportunities to study the tenuous atmospheres of Solar System satellites, revealing the presence and abundance of molecular and atomic species as well as their spatial and temporal variability. Far-UV aurorae have been used for decades to study the atmospheres of the galilean satellites. Here we present the first detection of Europa's visible-wavelength atomic oxygen aurora at 6300/6364 \AA{} arising from the metastable O$(^1$D) state, observed with the Keck I and Hubble Space Telescopes while Europa was in eclipse by Jupiter on six occasions in February-April 2018. The disk-integrated O($^1$D) brightness varies from $<$500 R up to more than 2 kR between dates, a factor of 15 higher than the OI 1356 \AA{} brightness on average. The ratio of emission at 6300/5577 \AA{} is diagnostic of parent molecule; the 5577 \AA{} emission was not detected in our dataset, which favors O$_2$ as the dominant atmospheric constituent and rules out an O/O$_2$ mixing ratio above 0.35. For an O$_2$ atmosphere and typical plasma conditions at Europa's orbit, the measured surface brightness range corresponds to column densities of 1-9$\times$10$^{14}$ cm$^{-2}$

The Applicability of Behaviour Change in Intervention Programmes Targeted at Ending Female Genital Mutilation in the EU:Integrating Social Cognitive and Community Level Approaches

With increased migration, female genital mutilation (FGM) also referred to as female circumcision or female genital cutting is no longer restricted to Africa, the Middle East, and Asia. The European Parliament estimates that up to half a million women living in the EU have been subjected to FGM, with a further 180,000 at risk. Aware of the limited success of campaigns addressing FGM, the World Health Organization recommended a behavioural change approach be implemented in order to end FGM. To date, however, little progress has been made in adopting a behaviour change approach in strategies aimed at ending FGM. Based on research undertaken as part of the EU’s Daphne III programme, which researched FGM intervention programmes linked to African communities in the EU (REPLACE), this paper argues that behaviour change has not been implemented due to a lack of understanding relating to the application of the two broad categories of behaviour change approach: individualistic decision-theoretic and community-change game-theoretic approaches, and how they may be integrated to aid our understanding and the development of future intervention strategies. We therefore discuss how these can be integrated and implemented using community-based participatory action research methods with affected communities

Health Benefits of Urban Agriculture

Health professionals increasingly recognize the value of farm-and garden-scale urban agriculture. Growing food and non-food crops in and near cities contributes to healthy communities by engaging residents in work and recreation that improves individual and public well-being. This article outlines the benefits of urban agriculture with regard to nutrition, food security, exercise, mental health, and social and physical urban environments. Potential risks are reviewed. Practical recommendations for health professionals to increase the positive benefits of urban agriculture are provided

Perceived abortion stigma and psychological well-being over five years after receiving or being denied an abortion.

OBJECTIVE:To prospectively assess perceptions of abortion stigma after receiving or being denied an abortion over 5 years, the factors associated with perceived abortion stigma, and the effects of perceived abortion stigma on psychological well-being. METHODS:We recruited people seeking abortion from 30 facilities across the US, and interviewed them by phone one week post-abortion seeking, then semiannually for 5 years. We used adjusted mixed effects regression analyses to examine the abortion stigma trajectories of those who obtained an abortion near a facility's gestational age limit (Near-limits) compared to those denied an abortion because they were just over the limit and carried their pregnancies to term (Turnaway-births). RESULTS:Of the 956 people recruited, we removed 28 due to ineligibility or missing data, leaving a final sample of 928. In unadjusted analyses, at one-week post-abortion seeking, over half of those seeking abortion perceived that if others knew they had sought an abortion, they would be looked down upon at least "a little bit" by people close to them (60%) or by people in their community (56%). In longitudinal adjusted analyses, people denied an abortion and who carried their pregnancies to term (Turnaway-birth group) reported significantly lower baseline perceived abortion stigma from people close to them (-0.38; 95% CI, -0.59, -0.16) and from people in their community (0.30; 95% CI, -0.52, -0.08), than Near-limits, differences that remained statistically significant for 1.5 years. Overall perceived abortion stigma declined significantly (p &lt; .001) for both study groups. High perceived abortion stigma at baseline was associated with higher odds of experiencing psychological distress years later (adjusted Odds Ratio, 3.98; 95% CI, 1.39, 11.37). CONCLUSIONS:Most people considering abortion perceive some abortion stigma, which is associated with psychological distress years later

Development of a Reinforcer Dimension Sensitivity Assessment to Inform Differential Reinforcement of Alternative BEhavior Without Extinction Procedures

Differential reinforcement of alternative behavior (DRA) is the most commonly used procedure to treat destructive behavior maintained by social-positive reinforcement (Petscher, Rey, & Bailey, 2009). Several studies have demonstrated that placing destructive behavior on extinction (EXT) during DRA is critical for the reduction of destructive behavior (Fisher et al., 1993; Hagopian, Fisher, Sullivan, Acquisto, & LeBlanc, 1998). Despite the empirical evidence demonstrating the importance of using EXT during DRA, the use of EXT has several limitations. These limitations have resulted in the development of DRA without EXT treatments, during which practitioners manipulate dimensions of reinforcement to favor appropriate responding. The systematic identification of reinforcer dimensions to which an individual’s behavior is sensitive for the purpose of informing subsequent DRA without EXT treatments is still relatively new. The purpose of this study was twofold. In Study 1, we developed a reinforcer dimension sensitivity assessment to identify parametric values of reinforcer dimensions to which a participant’s behavior was sensitive. We conducted Study 2 with participants whose behavior demonstrated sensitivity during the assessment. In Study 2 we conducted a validation analysis during which we implemented two DRA without EXT procedures to assess if the participants allocated responding towards the response that produced the optimal reinforcer parameter to which he demonstrated sensitivity during the reinforcer dimension sensitivity assessment

Synechococcus distribution and abundance in the San Juan Archipelago, Salish Sea

Synechococcus, a unicellular cyanobacterium of about one micron in size, is one of the most prolific and abundant primary producers worldwide and, hence, has an important role in the phytoplankton community. This study sought to determine 1) the distribution and abundance of Synechococcus in the eastern San Juan Archipelago; 2) the environmental variables related most closely to abundance; and 3) the key grazers of Synechococcus in this ecosystem. Two stations were chosen, East Sound near Orcas Island, WA and Rosario Strait near Lopez Pass, for their differing hydrographic conditions. Sampling was conducted from June to September 2012. Water samples were taken at three depths at both stations twice a month June through August, and then approximately every three days for three weeks in September. A CTD (Conductivity, Temperature, and Depth) was lowered at each station to obtain environmental data from the water column. Water samples were used for nutrient analysis, size-fractionated chlorophyll a analysis, and for the enumeration of Synechococcus and the protist grazer community. Synechococcus abundance rose as high as 1.5 x 104 cells ml-1 at both East Sound and Rosario Strait in August. Synechococcus abundance and depth distribution were nearly the same at both stations despite the well-mixed environment at Rosario and the more frequently stratified environment at East Sound. Both stations were abundant in nitrate+nitrite and phosphate throughout the sampling period. However, chlorophyll a concentrations were unusually low July through August, a season that usually exhibits variable and episodically high concentrations. Of all the environmental variables analyzed, only salinity was correlated with Synechococcus abundance at both stations, and that correlation was negative. The importance of salinity as a predictor of abundance may be due to a physiological effect of fresher water that allows for increased biomass production, or simply to the dominant effect of salinity on water column stratification, which may provide a preferable growth environment for Synechococcus. Ciliates, heterotrophic nanoflagellates, and dinoflagellates were observed with ingested Synechococcus. Surprisingly, nanoflagellates were rarely observed with ingested cells. Dinoflagellates seemed to be the key grazers of Synechococcus in the eastern San Juan Archipelago, but there was no clear temporal pattern to the level of Synechococcus ingestion by any of the aforementioned grazers