4,717 research outputs found

    Pressure Dependence of Fragile-to-Strong Transition and a Possible Second Critical Point in Supercooled Confined Water

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    By confining water in nano-pores of silica glass, we can bypass the crystallization and study the pressure effect on the dynamical behavior in deeply supercooled state using neutron scattering. We observe a clear evidence of a cusp-like fragile-to-strong (F-S) dynamic transition. Here we show that the transition temperature decreases steadily with an increasing pressure, until it intersects the homogenous nucleation temperature line of bulk water at a pressure of 1600 bar. Above this pressure, it is no longer possible to discern the characteristic feature of the F-S transition. Identification of this end point with the possible second critical point is discussed.Comment: 4 pages, 3 figure

    A case study in successful management of a data-poor fishery using simple decision rules: the Queensland spanner crab fishery

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    The Queensland spanner crab Ranina ranina is the target of a relatively data-poor, low-value fishery that has been managed for the last decade by using total allowable catches (TACs) in an individual transferable quota system. Despite the fact that this management system is usually applied to data-rich fisheries, it has been successfully used on this data-poor fishery. The key factor has been the use of harvest strategies that consisted only of simple decision rules that were appropriate given the size of the fishery and knowledge of the resource. These strategies were tested in a management strategy evaluation framework; however, it was not traditional in that (1) the operating model (or “true” resource to be managed) was not conditioned to data but rather was set to parameter ranges seen as appropriate for the resource and (2) the TAC was not set by using a stock assessment model, so the magnitude of the stock biomass was unknown. The important test was whether one could develop harvest strategies that were robust to this large uncertainty in knowledge by using only commercial catch rates. The management system had to be adaptive over time as more was learned about the biology of the species and how the harvest strategies affected the management of the fishery. This meant that the TAC was almost always set using the harvest strategies, but modifications to the decision rules were made on several occasions as more was learned about the fishery. The transparency and simplicity of the rules mean that the industry was empowered to make significant contributions to fine tuning the harvest strategies. As a result, the process does not rely solely on scientific advances but on the pooled knowledge of scientists, industry, and managers in a cooperative environment

    USE OF INHALANT ANESTHETICS IN THREE SNAKE SPECIES

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    Different snake species respond differently to various anesthetic agents. Hence, an anesthetic procedure developed for one species cannot necessarily be safely transferred to another species. The goal of this paper is to summarize our experience using inhalant anesthetics on three snake species, including both procedures that were successful and those we found to be less satisfactory. We found isoflurane delivered with a precision vaporizer to be the best agent to anesthetize black rat snakes (Elaphe o. obsoleta). Sex and mass did not seem to affect induction times in black rat snakes, but larger female rat snakes recovered faster from anesthesia than smaller females. Halothane delivered in the open method provided consistent anesthesia in northern water snakes (Nerodia s. sipedon), although it caused some mortality and should not be used on debilitated patients. Halothane delivered with a precision vaporizer may be used to anesthetize eastern massasauga rattlesnakes (Sistrurus c. catenatus). However, care must be taken to prevent mortality resulting from anesthetic overdose. Sex and mass had no effect on induction and recovery times in the rattlesnakes, but stressed animals require longer induction and recovery times

    The Spectroscopically Determined Substellar Mass Function of the Orion Nebula Cluster

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    We present a spectroscopic study of candidate brown dwarf members of the Orion Nebula Cluster (ONC). We obtained new J- and/or K-band spectra of ~100 objects within the ONC which are expected to be substellar based on their K,(H-K) magnitudes and colors. Spectral classification in the near-infrared of young low mass objects is described, including the effects of surface gravity, veiling due to circumstellar material, and reddening. From our derived spectral types and existing near-infrared photometry we construct an HR diagram for the cluster. Masses are inferred for each object and used to derive the brown dwarf fraction and assess the mass function for the inner 5.'1 x 5.'1 of the ONC, down to ~0.02 solar masses. The derived logarithmic mass function rises to a peak at ~0.2 solar masses, similar to previous IMF determinations derived from purely photometric methods, but falls off more sharply at the hydrogen-burning limit before leveling through the substellar regime. We compare the mass function derived here for the inner ONC to those presented in recent literature for the sparsely populated Taurus cloud members and the rich cluster IC 348. We find good agreement between the shapes and peak values of the ONC and IC 348 mass distributions, but little similarity between the ONC and Taurus results.Comment: Accepted for Publication in Apj. Added Erratu

    Cortisol responses enhance negative valence perception for ambiguous facial expressions

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    Stress exposure elicits a prolonged neuroendocrine response, marked by cortisol release, which can influence important forms of affective decision-making. Identifying how stress reactivity shapes subjective biases in decisions about emotional ambiguity (i.e., valence bias) provides insight into the role stress plays in basic affective processing for healthy and clinical populations alike. Here, we sought to examine how stress reactivity affects valence decisions about emotional ambiguity. Given that stress prioritizes automatic emotional processing which, in the context of valence bias, is associated with increased negativity, we tested how individual differences in acute stress responses influence valence bias and how this decision process evolves over time. Participants provided baseline ratings of clear (happy, angry) and ambiguous (surprised) facial expressions, then re-rated similar stimuli after undergoing an acute stress or control manipulation a week later; salivary cortisol was measured throughout to assay stress reactivity. Elevations in cortisol were associated with more negative ratings of surprised faces, and with more direct response trajectories toward negative ratings (i.e., less response competition). These effects were selectively driven by the stress group, evidencing that increased stress reactivity is associated with a stronger negativity bias during ambiguous affective decision-making

    Reappraisal—but not Suppression—Tendencies Determine Negativity Bias After Laboratory and Real‑World Stress Exposure

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    Higher reactivity to stress exposure is associated with an increased tendency to appraise ambiguous stimuli as negative. However, it remains unknown whether tendencies to use emotion regulation strategies—such as cognitive reappraisal, which involves altering the meaning or relevance of affective stimuli—can shape individual differences regarding how stress affects perceptions of ambiguity. Here, we examined whether increased reappraisal use is one factor that can determine whether stress exposure induces increased negativity bias. In Study 1, healthy participants (n = 43) rated the valence of emotionally ambiguous (surprised) faces before and after an acute stress or control manipulation and reported reappraisal habits. Increased negativity ratings were milder for stressed individuals that reported more habitual reappraisal use. In Study 2 (n = 97), we extended this investigation to real-world perceived stress before and during the COVID-19 pandemic. We found that reappraisal tendency moderates the relationship between perceived stress and increased negativity bias. Collectively, these findings suggest that the propensity to reappraise determines negativity bias when evaluating ambiguity under stress

    Stochastic oscillations of adaptive networks: application to epidemic modelling

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    Adaptive-network models are typically studied using deterministic differential equations which approximately describe their dynamics. In simulations, however, the discrete nature of the network gives rise to intrinsic noise which can radically alter the system's behaviour. In this article we develop a method to predict the effects of stochasticity in adaptive networks by making use of a pair-based proxy model. The technique is developed in the context of an epidemiological model of a disease spreading over an adaptive network of infectious contact. Our analysis reveals that in this model the structure of the network exhibits stochastic oscillations in response to fluctuations in the disease dynamic.Comment: 11 pages, 4 figure

    Purine biosynthesis in archaea: variations on a theme

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    <p>Abstract</p> <p>Background</p> <p>The ability to perform <it>de novo </it>biosynthesis of purines is present in organisms in all three domains of life, reflecting the essentiality of these molecules to life. Although the pathway is quite similar in eukaryotes and bacteria, the archaeal pathway is more variable. A careful manual curation of genes in this pathway demonstrates the value of manual curation in archaea, even in pathways that have been well-studied in other domains.</p> <p>Results</p> <p>We searched the Integrated Microbial Genome system (IMG) for the 17 distinct genes involved in the 11 steps of <it>de novo </it>purine biosynthesis in 65 sequenced archaea, finding 738 predicted proteins with sequence similarity to known purine biosynthesis enzymes. Each sequence was manually inspected for the presence of active site residues and other residues known or suspected to be required for function.</p> <p>Many apparently purine-biosynthesizing archaea lack evidence for a single enzyme, either glycinamide ribonucleotide formyltransferase or inosine monophosphate cyclohydrolase, suggesting that there are at least two more gene variants in the purine biosynthetic pathway to discover. Variations in domain arrangement of formylglycinamidine ribonucleotide synthetase and substantial problems in aminoimidazole carboxamide ribonucleotide formyltransferase and inosine monophosphate cyclohydrolase assignments were also identified.</p> <p>Manual curation revealed some overly specific annotations in the IMG gene product name, with predicted proteins without essential active site residues assigned product names implying enzymatic activity (21 proteins, 2.8% of proteins inspected) or Enzyme Commission (E. C.) numbers (57 proteins, 7.7%). There were also 57 proteins (7.7%) assigned overly generic names and 78 proteins (10.6%) without E.C. numbers as part of the assigned name when a specific enzyme name and E. C. number were well-justified.</p> <p>Conclusions</p> <p>The patchy distribution of purine biosynthetic genes in archaea is consistent with a pathway that has been shaped by horizontal gene transfer, duplication, and gene loss. Our results indicate that manual curation can improve upon automated annotation for a small number of automatically-annotated proteins and can reveal a need to identify further pathway components even in well-studied pathways.</p> <p>Reviewers</p> <p>This article was reviewed by Dr. CĂ©line Brochier-Armanet, Dr Kira S Makarova (nominated by Dr. Eugene Koonin), and Dr. Michael Galperin.</p

    Isolation and molecular characterization of the Romaine lettuce phylloplane mycobiome

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    Romaine lettuce (Lactuca sativa) is an important staple of American agriculture. Unlike many vegetables, romaine lettuce is typically consumed raw. Phylloplane microbes occur naturally on plant leaves; consumption of uncooked leaves includes consumption of phylloplane microbes. Despite this fact, the microbes that naturally occur on produce such as romaine lettuce are for the most part uncharacterized. In this study, we conducted culture-based studies of the fungal romaine lettuce phylloplane community from organic and conventionally grown samples. In addition to an enumeration of all such microbes, we define and provide a discussion of the genera that form the “core” romaine lettuce mycobiome, which represent 85.5% of all obtained isolates: Alternaria, Aureobasidium, Cladosporium, Filobasidium, Naganishia, Papiliotrema, Rhodotorula, Sampaiozyma, Sporobolomyces, Symmetrospora and Vishniacozyma. We highlight the need for additional mycological expertise in that 23% of species in these core genera appear to be new to science and resolve some taxonomic issues we encountered during our work with new combinations for Aureobasidiumbupleuri and Curvibasidium nothofagi. Finally, our work lays the ground for future studies that seek to understand the effect these communities may have on preventing or facilitating establishment of exogenous microbes, such as food spoilage microbes and plant or human pathogens
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