Oznaczanie histaminy w konserwach rybnych metoda kolorymetryczna wg Hardy-Smitha

Metodą kolorymetryczną wg Hardy-Smitha, uwzględniającą etap oczyszczania wyciągu z konserwy na kolumnie jonowymiennej, oznaczono zawartość histaminy w 79 próbkach konserw rybnych. W 18% zbadanych próbek konserw (sardynki jugosłowiańskie) zawartość histaminy przekraczała 20 mg/100 g produktu

High pressure processing of spices in atmosphere of helium for decrease of microbiological contamination

Celem pracy było zbadanie możliwości zastosowania technologii wysokociśnieniowej (HPP) oraz podwyższonej temperatury w atmosferze gazu szlachetnego - helu - do redukcji zanieczyszczenia mikrobiologicznego w przyprawach ziołowych: owocach kolendry i nasionach kminku. Badane produkty poddawano działaniu ciśnienia 800 MPa i 1 000 MPa przez 30 minut w zakresie temperatur od 60 do 121°C. Stwierdzono redukcję mikroflory tlenowej o 2 cykle logarytmiczne, przy całkowitej inaktywacji bakterii z grupy coli oraz drożdży i pleśni. Zaobserwowano, że skuteczność procesu w atmosferze helu zależy od wilgotności środowiska i zabieg pozwala na redukcję zanieczyszczenia mikrobiologicznego przynajmniej przy 20% zawartości wody. Zastosowanie procesu HPP w atmosferze helu może być wykorzystanie do poprawy jakości mikrobiologicznej przypraw ziołowych.The aim of the study was to investigate the microbiological decontamination of coriander and caraway when HPP technology was applied in elevated temperature in helium atmosphere. The HPP and heat treatment was conducted for 30 minutes at 800 and 1 000 MPa and temperature range was 60 - 121°C. Contamination with aerobic mesophilic bacteria was decreased by about 2 logarithmic cycles. Total elimination of coliform and yeast and moulds was observed. The efficacy of HPP treatment under helium atmosphere depended on the content of the water in tested samples. It can be concluded that high pressure treatment under atmosphere of helium, combination of proper high pressure and time improved the microbiological quality of spices

Design, recruitment, logistics, and data management of the GEHA (Genetics of Healthy Ageing) project

International audienceIn 2004, the integrated European project GEHA (Genetics of Healthy Ageing) was initiated with the aim of identifying genes involved in healthy ageing and longevity. The first step in the project was the recruitment of more than 2500 pairs of siblings aged 90 years or more together with one younger control person from 15 areas in 11 European countries through a coordinated and standardised effort. A biological sample, preferably a blood sample, was collected from each participant, and basic physical and cognitive measures were obtained together with information about health, life style, and family composition.From 2004 to 2008 a total of 2535 families comprising 5319 nonagenarian siblings were identifiedand included in the project. In addition, 2548 younger control persons aged 50–75 years were recruited. A total of 2249 complete trios with blood samples from at least two old siblings and the younger control were formed and are available for genetic analyses (e.g. linkage studies and genome-wide association studies).Mortality follow-up improves the possibility of identifying families with the most extreme longevity phenotypes. With a mean follow-up time of 3.7 years the number of families with all participating siblings aged 95 years or more has increased by a factor of 5 to 750 families compared to when interviews were conducted. Thus, the GEHA project represents a unique source in the search for genes related to healthy ageing and longevity