Two primary methods of proving gas flow meters

Methods for determining mass flow rates of gases for use in calibrating gas flowmeter

Differential expression of microRNAs in bovine papillomavirus type 1 transformed equine cells

Bovine papillomavirus (BPV) types 1 and 2 play an important role in the pathogenesis of equine sarcoids (ES), the most common cutaneous tumour affecting horses. MicroRNAs (miRNAs), small non-coding RNAs that regulate essential biological and cellular processes, have been found dysregulated in a wide range of tumours. The aim of this study was to identify miRNAs associated with ES. Differential expression of miRNAs was assessed in control equine fibroblasts (EqPalFs) and EqPalFs transformed with the BPV-1 genome (S6-2 cells). Using a commercially available miRNA microarray, 492 mature miRNAs were interrogated. In total, 206 mature miRNAs were differentially expressed in EqPalFs compared with S6-2 cells. Aberrant expression of these miRNAs in S6-2 cells can be attributed to the presence of BPV-1 genomes. Furthermore, we confirm the presence of 124 miRNAs previously computationally predicted in the horse. Our data supports the involvement of miRNAs in the pathogenesis of ES

Study of a Large NaI(Tl) Crystal

Using a narrow band positron beam, the response of a large high-resolution NaI(Tl) crystal to an incident positron beam was measured. It was found that nuclear interactions cause the appearance of additional peaks in the low energy tail of the deposited energy spectrum

Electrostatics of Gapped and Finite Surface Electrodes

We present approximate methods for calculating the three-dimensional electric potentials of finite surface electrodes including gaps between electrodes, and estimate the effects of finite electrode thickness and an underlying dielectric substrate. As an example we optimize a radio-frequency surface-electrode ring ion trap, and find that each of these factors reduces the trapping secular frequencies by less than 5% in realistic situations. This small magnitude validates the usual assumption of neglecting the influences of gaps between electrodes and finite electrode extent.Comment: 9 pages, 9 figures (minor changes

A conceptual design for the Thirty Meter Telescope adaptive optics systems

In this paper, we provide an overview of the adaptive optics (AO) program for the Thirty Meter Telescope (TMT) project, including an update on requirements; the philosophical approach to developing an overall AO system architecture; the recently completed conceptual designs for facility and instrument AO systems; anticipated first light capabilities and upgrade options; and the hardware, software, and controls interfaces with the remainder of the observatory. Supporting work in AO component development, lab and field tests, and simulation and analysis is also discussed. Further detail on all of these subjects may be found in additional papers in this conference

Mechanical testing and microstructural analysis of wire arc additively manufactured steels

Wire arc additive manufacturing (WAAM) is a metal 3D printing method that allows the cost-effective and efficient production of large-scale elements, and has thus gained great interest from architects and structural engineers. Integration of this novel technology into the construction industry, however, requires the development of a clear understanding of the mechanical behaviour of WAAM materials. To this end, a comprehensive experimental study into the mechanical properties and microstructure of WAAM plates made of normal- and high-strength steels has been undertaken and is reported herein. A total of 137 as-built and machined tensile coupons were tested, extracted in various directions relative to the print layer orientation from WAAM plates of two nominal thicknesses, built using different deposition strategies. The influence of the geometric undulations inherent to the WAAM process and deposition strategy on the resulting mechanical properties was investigated. Microstructural characterisation was also performed by means of optical microscopy (OM) and electron backscatter diffraction (EBSD). The WAAM normal-strength steel plates exhibited a principally ferritic-pearlitic microstructure, while the WAAM high-strength steel plates displayed a mixed microstructure featuring ferrite, bainite and martensite. The EBSD analysis revealed a weak crystallographic texture, which explained the observed mechanical properties being almost isotropic. No significant differences in tensile properties were observed with the different deposition strategies, except for some variation in ductility. The geometric undulations of the as-built coupons resulted in some reduction in effective mechanical properties and a degree of anisotropy. Overall, the examined WAAM material exhibited consistent mechanical properties, a Young’s modulus comparable to conventionally-produced steel plates, marginally lower strength, reflecting the slower cooling conditions than is customary, and good ductility

The effect of worrying on intolerance of uncertainty and positive and negative beliefs about worry

Background and Objectives: The effect of a worry manipulation on the clinical constructs intolerance of uncertainty (IU), negative beliefs about the consequences of worry (NCOW), positive beliefs about the consequences of worry (PCOW), in addition to the emotions anxiety and sadness, was examined. Methods: A non-clinical sample was split into two groups, a worry group (n = 29), who were asked to generate 20 potential worries about a hypothetical scenario, and a control group (n = 28), who were asked to generate 2 potential worries about the same scenario. Subsequently, participants were asked to complete measures of IU, NCOW, PCOW, sadness and anxiety. Results: The worry group scored significantly higher than the control group on measures of IU, NCOW and PCOW but not on measures of sadness and anxiety. Limitations: Possible limitations of the current study include the use of a student sample and the use of a hypothetical worry scenario. Conclusions: The results suggest that engaging in worry can increase scores on measures of the beliefs and thought patterns often used to causally explain worry. The results are in line with recent research showing bidirectionality between anxiety related symptoms and their associated clinical constructs, and are consistent with an approach which sees anxiety symptoms as part of an evolved integrated threat management system that alerts the individual to threats to goals or challenges, and coordinates cognitive, behavioral, and affective reactions to enable effective responding to these threats and challenges

Expression and purification of an active cysteine protease of Haemonchus contortus using Caenorhabditis elegans

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