905 research outputs found
Scaling Behaviour and Complexity of the Portevin-Le Chatelier Effect
The plastic deformation of dilute alloys is often accompanied by plastic
instabilities due to dynamic strain aging and dislocation interaction. The
repeated breakaway of dislocations from and their recapture by solute atoms
leads to stress serrations and localized strain in the strain controlled
tensile tests, known as the Portevin-Le Chatelier (PLC) effect. In this present
work, we analyse the stress time series data of the observed PLC effect in the
constant strain rate tensile tests on Al-2.5%Mg alloy for a wide range of
strain rates at room temperature. The scaling behaviour of the PLC effect was
studied using two complementary scaling analysis methods: the finite variance
scaling method and the diffusion entropy analysis. From these analyses we could
establish that in the entire span of strain rates, PLC effect showed Levy walk
property. Moreover, the multiscale entropy analysis is carried out on the
stress time series data observed during the PLC effect to quantify the
complexity of the distinct spatiotemporal dynamical regimes. It is shown that
for the static type C band, the entropy is very low for all the scales compared
to the hopping type B and the propagating type A bands. The results are
interpreted considering the time and length scales relevant to the effect.Comment: 35 pages, 6 figure
Nanomechanical and thermophoretic analyses of the nucleotide-dependent interactions between the AAA+ subunits of magnesium chelatase
In chlorophyll biosynthesis, the magnesium
chelatase enzyme complex catalyzes the insertion of a Mg2+
ion into protoporphyrin IX. Prior to this event, two of the three
subunits, the AAA+ proteins ChlI and ChlD, form a ChlID−
MgATP complex. We used microscale thermophoresis to
directly determine dissociation constants for the I-D subunits
from Synechocystis, and to show that the formation of a ChlID−
MgADP complex, mediated by the arginine finger and the
sensor II domain on ChlD, is necessary for the assembly of the
catalytically active ChlHID−MgATP complex. The N-terminal
AAA+ domain of ChlD is essential for complex formation, but
some stability is preserved in the absence of the C-terminal
integrin domain of ChlD, particularly if the intervening polyproline linker region is retained. Single molecule force spectroscopy
(SMFS) was used to determine the factors that stabilize formation of the ChlID−MgADP complex at the single molecule level;
ChlD was attached to an atomic force microscope (AFM) probe in two different orientations, and the ChlI subunits were
tethered to a silica surface; the probability of subunits interacting more than doubled in the presence of MgADP, and we show
that the N-terminal AAA+ domain of ChlD mediates this process, in agreement with the microscale thermophoresis data. Analysis
of the unbinding data revealed a most probable interaction force of around 109 pN for formation of single ChlID−MgADP
complexes. These experiments provide a quantitative basis for understanding the assembly and function of the Mg chelatase
complex
Intercalation-enhanced electric polarization and chain formation of nano-layered particles
Microscopy observations show that suspensions of synthetic and natural
nano-layered smectite clay particles submitted to a strong external electric
field undergo a fast and extended structuring. This structuring results from
the interaction between induced electric dipoles, and is only possible for
particles with suitable polarization properties. Smectite clay colloids are
observed to be particularly suitable, in contrast to similar suspensions of a
non-swelling clay. Synchrotron X-ray scattering experiments provide the
orientation distributions for the particles. These distributions are understood
in terms of competing (i) homogenizing entropy and (ii) interaction between the
particles and the local electric field; they show that clay particles polarize
along their silica sheet. Furthermore, a change in the platelet separation
inside nano-layered particles occurs under application of the electric field,
indicating that intercalated ions and water molecules play a role in their
electric polarization. The resulting induced dipole is structurally attached to
the particle, and this causes particles to reorient and interact, resulting in
the observed macroscopic structuring. The macroscopic properties of these
electro-rheological smectite suspensions may be tuned by controlling the nature
and quantity of the intercalated species, at the nanoscale.Comment: 7 pages, 5 figure
Relaxation oscillations and negative strain rate sensitivity in the Portevin - Le Chatelier effect
A characteristic feature of the Portevin - Le Chatelier effect or the jerky
flow is the stick-slip nature of stress-strain curves which is believed to
result from the negative strain rate dependence of the flow stress. The latter
is assumed to result from the competition of a few relevant time scales
controlling the dynamics of jerky flow. We address the issue of time scales and
its connection to the negative strain rate sensitivity of the flow stress
within the framework of a model for the jerky flow which is known to reproduce
several experimentally observed features including the negative strain rate
sensitivity of the flow stress. We attempt to understand the above issues by
analyzing the geometry of the slow manifold underlying the relaxational
oscillations in the model. We show that the nature of the relaxational
oscillations is a result of the atypical bent geometry of the slow manifold.
The analysis of the slow manifold structure helps us to understand the time
scales operating in different regions of the slow manifold. Using this
information we are able to establish connection with the strain rate
sensitivity of the flow stress. The analysis also helps us to provide a proper
dynamical interpretation for the negative branch of the strain rate
sensitivity.Comment: 7 figures, To appear in Phys. Rev.
Porphyrin Binding to Gun4 protein, Facilitated by a Flexible Loop, Controls Metabolite Flow through the Chlorophyll Biosynthetic Pathway
In oxygenic phototrophs, chlorophylls, hemes and bilins are synthesized by a common branched pathway. Given the phototoxic nature of tetrapyrroles, this pathway must be tightly regulated and an important regulatory role is attributed to Mgchelatase enzyme at the branching between the heme and chlorophyll pathway. Gun4 is a porphyrin-binding protein known to stimulate in vitro the Mg-chelatase activity but how the Gun4-porphyrin complex acts in the cell was
unknown. To address this issue we first performed simulations to determine the porphyrin-docking mechanism to the
cyanobacterial Gun4 structure. After correcting crystallographic loop contacts, we determined the binding site for Mgprotoporphyrin IX. It revealed that the orientation of 6/7 loop is critical for the binding and the magnesium ion held within the porphyrin is coordinated by Asn211 residue. We also identified the basis for stronger binding in the Gun4-1 variant and for weaker binding in the W192A mutant. The W192A-Gun4 was further characterized in Mg-chelatase assay showing that tight porphyrin-binding in Gun4 facilitates its interaction with the Mg-chelatase ChlH
subunit. Finally, we introduced the W192A mutation into Synechocystis 6803 cells and show that the Gun4-porphyrin complex is important for the accumulation of ChlH and for channeling metabolites into the chlorophyll biosynthetic pathway.This work was supported by project P501/12/G055 of the Czech Science Foundation, and by the National Programme of Sustainability I (LO1416) and by ERC 2009-Adg25027-PELE (to V.G). J.K. was supported by project Algain (EE2.3.30.0059). N.B.P.A., P.A.D., A.A.B. and C.N.H. thank
the Biotechnology and Biological Sciences Research Council (BBSRC) U.K. for funding, under award numbers BB/G021546/1 and BB/M000265/1. CNH was also supported by an Advanced Award 338895 from the European Research Council.Peer ReviewedPostprint (author's final draft
Existence of radial stationary solutions for a system in combustion theory
In this paper, we construct radially symmetric solutions of a nonlinear
noncooperative elliptic system derived from a model for flame balls with
radiation losses. This model is based on a one step kinetic reaction and our
system is obtained by approximating the standard Arrehnius law by an ignition
nonlinearity, and by simplifying the term that models radiation. We prove the
existence of 2 solutions using degree theory
High order amplitude equation for steps on creep curve
We consider a model proposed by one of the authors for a type of plastic
instability found in creep experiments which reproduces a number of
experimentally observed features. The model consists of three coupled
non-linear differential equations describing the evolution of three types of
dislocations. The transition to the instability has been shown to be via Hopf
bifurcation leading to limit cycle solutions with respect to physically
relevant drive parameters. Here we use reductive perturbative method to extract
an amplitude equation of up to seventh order to obtain an approximate analytic
expression for the order parameter. The analysis also enables us to obtain the
bifurcation (phase) diagram of the instability. We find that while
supercritical bifurcation dominates the major part of the instability region,
subcritical bifurcation gradually takes over at one end of the region. These
results are compared with the known experimental results. Approximate analytic
expressions for the limit cycles for different types of bifurcations are shown
to agree with their corresponding numerical solutions of the equations
describing the model. The analysis also shows that high order nonlinearities
are important in the problem. This approach further allows us to map the
theoretical parameters to the experimentally observed macroscopic quantities.Comment: LaTex file and eps figures; Communicated to Phys. Rev.
A test of the ability of current bulk optical models to represent the radiative properties of cirrus cloud across the mid-and far-infrared
Measurements of mid- to far-infrared nadir radiances obtained from the UK Facility for Airborne Atmospheric Measurements (FAAM) BAe-146 aircraft during the Cirrus Coupled Cloud-Radiation Experiment (CIRCCREX) are used to assess the performance of various ice cloud bulk optical (single-scattering) property models. Through use of a minimisation approach, we find that the simulations can reproduce the observed spectra in the mid-infrared to within measurement uncertainty but are unable to simultaneously match the observations over the far-infrared frequency range. When both mid and far-infrared observations are used to minimise residuals, first order estimates of the flux differences between the best performing simulations and observations indicate a strong compensation effect between the mid and far infrared such that the absolute broadband difference is < 0.7 W m−2. However, simply matching the spectra using the mid-infrared observations in isolation leads to substantially larger discrepancies, with absolute differences reaching ~ 1.8 W m−2. These results highlight the benefit of far infrared observations for better constraining retrievals of cirrus cloud properties and their radiative impact, and provide guidance for the development of more realistic ice cloud optical models
Syntectonic mobility of supergene nickel ores of New Caledonia (Southwest Pacific). Evidence from faulted regolith and garnierite veins.
International audienceSupergene nickel deposits of New Caledonia that have been formed in the Neogene by weathering of obducted ultramafic rocks are tightly controlled by fracture development. The relationship of tropical weathering and tectonic structures, faults and tension gashes, have been investigated in order to determine whether fractures have play a passive role only, as previously thought; or alternatively, if brittle tectonics was acting together with alteration. From the observation of time-relationship, textures, and mineralogy of various fracture fills and fault gouges, it may be unambiguously established that active faulting has play a prominent role not only in facilitating drainage and providing room for synkinematic crystallisation of supergene nickel silicate, but also in mobilising already formed sparse nickel ore, giving birth to the very high grade ore nicknamed "green gold"
Overfeeding, Autonomic Regulation and Metabolic Consequences
The autonomic nervous system plays an important role in the regulation of body processes in health and disease. Overfeeding and obesity (a disproportional increase of the fat mass of the body) are often accompanied by alterations in both sympathetic and parasympathetic autonomic functions. The overfeeding-induced changes in autonomic outflow occur with typical symptoms such as adiposity and hyperinsulinemia. There might be a causal relationship between autonomic disturbances and the consequences of overfeeding and obesity. Therefore studies were designed to investigate autonomic functioning in experimentally and genetically hyperphagic rats. Special emphasis was given to the processes that are involved in the regulation of peripheral energy substrate homeostasis. The data revealed that overfeeding is accompanied by increased parasympathetic outflow. Typical indices of vagal activity (such as the cephalic insulin release during food ingestion) were increased in all our rat models for hyperphagia. Overfeeding was also accompanied by increased sympathetic tone, reflected by enhanced baseline plasma norepinephrine (NE) levels in both VMH-lesioned animals and rats rendered obese by hyperalimentation. Plasma levels of NE during exercise were, however, reduced in these two groups of animals. This diminished increase in the exercise-induced NE outflow could be normalized by prior food deprivation. It was concluded from these experiments that overfeeding is associated with increased parasympathetic and sympathetic tone. In models for hyperphagia that display a continuously elevated nutrient intake such as the VMH-lesioned and the overfed rat, this increased sympathetic tone was accompanied by a diminished NE response to exercise. This attenuated outflow of NE was directly related to the size of the fat reserves, indicating that the feedback mechanism from the periphery to the central nervous system is altered in the overfed state.
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