Kummiluokkatoiminnan arviointi

Tämän opinnäytetyön tarkoituksena oli kuvata ja arvioida kummiluokkatoimintaa ja sen vaikutuksia sairaanhoitajaopiskelijan/terveydenhoitajaopiskelijan opintoihin sekä työelämään pääsemiseen ja sopeutumiseen. Tämä opinnäytetyö toteutettiin empiirisenä tutkimuksena. Kummiluokkatoimintaa arvioitiin kyselyn avulla. Kysely tehtiin sähköisenä Webropol-kyselynä. Kysely lähetettiin sähköpostitse 55 hoitoalan opiskelijalle, jotka olivat olleet mukana kummiluokkatoiminnassa. Kyselyyn vastasi yhdeksän opiskelijaa, vastausprosentti oli 16,36 %. Kysely sisälsi sekä avoimia kysymyksiä että monivalintakysymyksiä. Vastausten analysointiin käytettiin sisällönanalyysiä sekä tilastointia. Kyselyn tulokseksi saatiin, että opiskelijat eivät pääosin olleet tyytyväisiä kummiluokkatoimintaan sellaisena kuin se oli toteutunut. Kummiluokkatapaamisia oli ollut liian vähän, eikä niillä ollut vaikutusta työn saantiin. Kuitenkin se toiminta, mitä opiskelijoille oli tarjottu, oli mieleistä. Muutaman opiskelijan mielestä toiminta oli lisännyt halua työskennellä Varsinais-Suomen sairaanhoitopiirissä ja yhden mielestä se oli tehnyt työnhausta helpompaa. Myös vierailukäynnit ja esitykset olivat olleet kiinnostavia, niitä olisi vain pitänyt olla enemmän. Tämän opinnäytetyön tuomaa tietoa voidaan käyttää kummiluokkatoiminnan kehittämiseen, jotta hoitoalan opiskelijat saadaan yhä enemmän sitoutumaan ja kiinnostumaan tulevasta ammatistaan. Saadut tulokset antavat myös lisää syitä kummiluokkatoiminnan jatkamiselle. Jatkotutkimuksina voisi selvittää, miten kummiluokkatoiminta vaikuttaa Turun Yliopistolliseen sairaalaan ja VSSHP:in, eli hyötyykö toiminnasta opiskelijoiden lisäksi myös organisaatio.The purpose of this study was to describe and evaluate the godparent activities and its effects on nursing studies, as well as to work to reach and adjust. This thesis has been implemented by empirical research. Godparent activity was assessed using an inquiry. The inquiry was conducted by Webropol survey. The inquiry was sent by e-mail of 55 nursing students, who had been involved in godparent activities. Nine students responded to the inquiry, the response rate was 16.36%. The inquiry contained both open questions and multiple choice questions. Content analysis and statistics were used for the analysis of the responses. The inquiry led to the result that students are not generally been satisfied with the godparent activities as it had been. Godparent meetings were only few, nor did they have the effect of employment. However, it is low, what activities were offered to students was worthwhile. A few view, the activities had added desire to work in Hospital District of Southwest Finland, and one thought it was done work search easier. Also, visits and information lectures were interesting; they would only have been more. This thesis can be used to develop the godparent activities, to get nursing students to increasingly commit to and interested in their future profession. The information obtained will also provide more reasons to continuation of godparent activities. Possible further studies could figure out how godparent activities affect the Turku University Hospital and in Hospital District of Southwest Finland, to benefit for the students, but also the organization

Click-based synthesis and proteomic profiling of lipstatin analogues

Using click chemistry to enable both structural diversity and proteome profiling within a natural product derived library, two out of nineteen lipstatin analogues showed similar activity to Orlistat against fatty acid synthase (FAS), but with an improved ability to induce tumour cell death

Extended ubiquitin species are protein-based DUB inhibitors

A frame-shift mutation in the transcript of the ubiquitin-B gene leads to a C-terminally extended ubiquitin, UBB+1. UBB+1 has been considered to inhibit proteasomes, and as such to be the underlying cause for toxic protein buildup correlated with certain neuropathological conditions. We demonstrated that expression of extended ubiquitin variants led to accumulation of heterogeneously-linked polyubiquitin conjugates indicating a pervasive effect on ubiquitin-dependent turnover. 20S proteasomes selectively proteolysed ubiquitin extensions, yet no evidence for inhibition of 26S holoenzymes was found. However, among susceptible targets for inhibition was Ubp6, the primary enzyme responsible for disassembly of lysine-48 linkages at 26S proteasomes. Processing of lysine-48 and lysine-63 linkages by other deubiquitinating enzymes (DUBs) was also inhibited. Disruption of ubiquitin-dependent degradation by extended ubiquitin variants may therefore be attributed to their inhibitory effect on select DUBs, thus shifting research efforts related to protein accumulation in neurodegenerative processes from proteasomes to DUBs

Optical second harmonic generation in Yttrium Aluminum Borate single crystals (theoretical simulation and experiment)

Experimental measurements of the second order susceptibilities for the second harmonic generation are reported for YAl3(BO3)4 (YAB) single crystals for the two principal tensor components xyz and yyy. First principles calculation of the linear and nonlinear optical susceptibilities for Yttrium Aluminum Borate YAl3(BO3)4 (YAB) crystal have been carried out within a framework of the full-potential linear augmented plane wave (FP-LAPW) method. Our calculations show a large anisotropy of the linear and nonlinear optical susceptibilities. The observed dependences of the second order susceptibilities for the static frequency limit and for the frequency may be a consequence of different contribution of electron-phonon interactions. The imaginary parts of the second order SHG susceptibility chi_{123}^{(2)}(omega), chi_{112}^{(2)}(omega), chi_{222}^{(2)}(omega), and chi_{213}^{(2)}(omega) are evaluated. We find that the 2(omega) inter-band and intra-band contributions to the real and imaginary parts of chi_{ijk}^{(2)}\l(omega) show opposite signs. The calculated second order susceptibilities are in reasonably good agreement with the experimental measurements.Comment: 16 pages, 11 figure

svmPRAT: SVM-based Protein Residue Annotation Toolkit

<p>Abstract</p> <p>Background</p> <p>Over the last decade several prediction methods have been developed for determining the structural and functional properties of individual protein residues using sequence and sequence-derived information. Most of these methods are based on support vector machines as they provide accurate and generalizable prediction models.</p> <p>Results</p> <p>We present a general purpose protein residue annotation toolkit (<it>svm</it><monospace>PRAT</monospace>) to allow biologists to formulate residue-wise prediction problems. <it>svm</it><monospace>PRAT</monospace> formulates the annotation problem as a classification or regression problem using support vector machines. One of the key features of <it>svm</it><monospace>PRAT</monospace> is its ease of use in incorporating any user-provided information in the form of feature matrices. For every residue <it>svm</it><monospace>PRAT</monospace> captures local information around the reside to create fixed length feature vectors. <it>svm</it><monospace>PRAT</monospace> implements accurate and fast kernel functions, and also introduces a flexible window-based encoding scheme that accurately captures signals and pattern for training effective predictive models.</p> <p>Conclusions</p> <p>In this work we evaluate <it>svm</it><monospace>PRAT</monospace> on several classification and regression problems including disorder prediction, residue-wise contact order estimation, DNA-binding site prediction, and local structure alphabet prediction. <it>svm</it><monospace>PRAT</monospace> has also been used for the development of state-of-the-art transmembrane helix prediction method called TOPTMH, and secondary structure prediction method called YASSPP. This toolkit developed provides practitioners an efficient and easy-to-use tool for a wide variety of annotation problems.</p> <p><it>Availability</it>: <url>http://www.cs.gmu.edu/~mlbio/svmprat</url></p

New thermodynamic data for CoTiO3, NiTiO3 and CoCO3 based on low-temperature calorimetric measurements

The low-temperature heat capacities of nickel titanate (NiTiO3), cobalt titanate (CoTiO3), and cobalt carbonate (CoCO3) were measured between 2 and 300 K, and thermochemical functions were derived from the results. Our new data show previously unknown low-temperature lambda-shaped heat capacity anomalies peaking at 37 K for CoTiO3 and 26 K for NiTiO3. From our data we calculate standard molar entropies (298.15 K) for NiTiO3 of 90.9 ± 0.7 J mol-1 K-1 and for CoTiO3 of 94.4 ± 0.8 J mol-1 K-1. For CoCO3, we find only a small broad heat capacity anomaly, peaking at about 31 K. From our data, we suggest a new standard entropy (298.15 K) for CoCO3 of 88.9 ± 0.7 J mol-1 K-1

Chemical Approaches To Perturb, Profile, and Perceive Glycans

Glycosylation is an essential form of post-translational modification that regulates intracellular and extracellular processes. Regrettably, conventional biochemical and genetic methods often fall short for the study of glycans, because their structures are often not precisely defined at the genetic level. To address this deficiency, chemists have developed technologies to perturb glycan biosynthesis, profile their presentation at the systems level, and perceive their spatial distribution. These tools have identified potential disease biomarkers and ways to monitor dynamic changes to the glycome in living organisms. Still, glycosylation remains the underexplored frontier of many biological systems. In this Account, we focus on research in our laboratory that seeks to transform the study of glycan function from a challenge to routine practice