138 research outputs found

    A minimal cytomegalovirus intron A variant can improve transgene expression in different mammalian cell lines.

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    Made available in DSpace on 2018-07-01T01:26:14Z (GMT). No. of bitstreams: 1 Quilici2013ArticleAMinimalCytomegalovirusIntronA.pdf: 267916 bytes, checksum: a0678d07681cb4ac9d04a72f07b2d182 (MD5) Previous issue date: 2013-08-12bitstream/item/179295/1/Quilici2013-Article-AMinimalCytomegalovirusIntronA.pd

    Estereótipos sociais do idoso para diferentes grupos etários

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    The aim of this study was to identify stereotypes of elderly for different age groups. Eight focus groups withfive participants each were conducted. 12 pictures of persons and 70 cards were used with adjectives regarding to elderlies.The participants (40) were separated by gender and age groups. Among them, 30 reported daily contact with elderlies. For theelderly participants, age was the main criterion of categorization, followed by ethnicity, for the other groups the main criterionwas gender, followed by age. In average, each group developed three categories and all reported age as the used criterion.Consensus of categories emerged on “old-aged”: cult, depressed, grumpy, positive and negative. The homogeneity effect ofthe out-group occurred in most groups, but the intergroup favoritism did not take place.O objetivo deste estudo foi identificar estereótipos de idosos para diferentes grupos etários. Foram realizados oito grupos focais com cinco integrantes cada. Foram utilizadas 12 fotografias de pessoas e 70 cartões com adjetivos atribuídos ao idoso. Os participantes (40) foram divididos por sexo e grupo etário. Destes, 30 relataram contato diário com idosos. Para os idosos, idade foi o principal critério de categorização, seguido de etnia. Para os outros grupos, o principal critério foi sexo, seguido de idade. Em média, cada grupo constituiu três categorias, todos evidenciaram a idade como critério utilizado. Categorias consensuais emergiram sobre "idosos": culto, depressivo, ranzinza, positivo e negativo. O efeito de homogeneidade do out-group ocorreu na maioria dos grupos, mas o favoritismo intergrupal não aconteceu

    Glossario de biotecnologia vegetal.

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    Representações sociais da internet para idosos

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    Introduction: Increase in Internet access by elderly people is a consequence of population ageing, even though a long way still lies ahead for their digital inclusion. Objective: To describe the social representations of Internet among the elderly  and to compare objectification and anchoring processes of elderly people with different levels in Internet usage. Methods: Qualitative and quantitative study, with descriptive and comparative design, involving forty participants. Data collection occurred through  different tools as follows: 1) associative network, analyzed by EVOC2000 and calculation of polarity 2) semi-structured interview, analyzed by Descending Hierarchical Classification with IRaMuTeQ and  content analysis by Atlas TI and  3) characterization questionnaire and evaluation scale for digital inclusion level,   with  descriptive statistical analysis by SPSS software. Results: Associative network analyzed 78 words and their polarity was slightly positive. Descending Hierarchical Classification analyzed 89.51% of the corpus, divided into three segments: Internet danger, difficulties in usage v. Internet options and practices; content analysis divided 505 occurrences into three categories: image, attitude and information. Conclusion: Representations of elderly people with the highest use of Internet were undertaken by accessing hardware, apps and sites, anchored on an idea of the Internet as a means of retrieving information, leisure and interaction. Representations of the elderly with the lowest level of experience were undertaken by computer, based on a sociological perspective and marked by unfavorable attitudes.Introdução: O aumento do acesso à internet por idosos acompanha o avanço do envelhecimento populacional, mas há ainda um longo caminho a percorrer para a inclusão digital desse coletivo. Objetivo: Descrever as representações sociais da internet para idosos e comparar seus processos de objetificação e ancoragem para idosos com diferentes níveis de uso da internet. Método: Trata-se de um estudo qualitativo e quantitativo, com delineamento descritivo e comparativo, composto por 40 participantes. A coleta de dados ocorreu por rede associativa, analisada pelo programa EVOC2000 e cálculo de polaridade, entrevista semiestruturada, analisada por Classificação Hierárquica Descendente com o software IRaMuTeQ e análise de conteúdo por meio do software Atlas.TI, questionário de caracterização e escala de avaliação do nível de inclusão digital, analisados através de análise estatística descritiva do Pacote Estatístico SPSS. Resultados: A rede associativa analisou 78 palavras e sua polaridade foi levemente positiva. A Classificação Hierárquica Descendente analisou 89,51% do corpus e o separou em 3 segmentos de texto: o perigo da internet, dificuldades de uso x escolha e práticas na internet e a análise de conteúdo dividiu 505 ocorrências em três categorias: imagem, atitude e informação. Conclusão: As representações dos idosos com maior nível de uso da internet foram objetificadas por meio de hardwares de acesso, aplicativos e sites e ancoradas na noção da internet como forma de obter informação, lazer e interação. Enquanto as representações dos idosos com menor nível de experiência foram objetificadas por meio do computador, ancorada em uma perspectiva sociológica, marcada por atitudes desfavoráveis

    Myrteae phylogeny, calibration, biogeography and diversification patterns: increased understanding in the most species rich tribe of Myrtaceae

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    Myrteae (c. 2500 species; 51 genera) is the largest tribe of Myrtaceae and an ecologically important groups of angiosperms in the Neotropics. Systematic relationships in Myrteae are complex, hindering conservation initiatives and jeopardizing evolutionary modelling. A well-supported and robust phylogenetic hypothesis was here targeted towards a comprehensive understanding of the relationships within the tribe. The resultant topology was used as a base for key evolutionary analyses such as age estimation, historical biogeography and diversification rate patterns. One nuclear (ITS) and seven chloroplast (psbAtrnH, matIC, ndhF, trnl-trnF, trnQ-rps16, rpl16 and rpl32-trnL) DNA regions for 115 taxa representing 46 out of the 51 genera in the tribe were accessed and analysed using maximum likelihood and Bayesian inference tools for phylogenetic reconstruction. Dates of diversification events were estimated and contrasted using two distinct fossil sets (macro and pollen) in BEAST. The subsequent dated phylogenies were compared and analysed for biogeographical patterns using BioGeoBEARS and diversification rates using BAMM. Myrteae phylogeny presents strong statistical support for three major clades within the tribe: Australasian group, Myrtus group and Main Neotropical Lineage. Dating results from calibration using macrofossil are an average of 20 million years older and show an early Paleocene origin of Myrteae, against a mid-Eocene one from the pollen fossil calibration. Biogeographic analysis shows the origin of Myrteae in Zealandia in both calibration approaches, followed by a widespread distribution throughout the still-linked Gondwana continents and diversification of Neotropical endemic lineages by later vicariance. Best configuration shift indicates three points of acceleration in diversification rates, all of them occurring in the Main Neotropical Lineage. Based on the reconstructed topology, several new taxonomic placements were recovered, including: the relative position of Myrtus communis, the placement of the Blepharocalyx group, the absence of generic endemism in the Caribbean, and the paraphyletism of the former Pimenta group. Distinct calibration approaches affect biogeography interpretation, increasing the number of necessary long distance dispersal events in the topology with older nodes. It is hypothesised that biological intrinsic factors such as modifications of embryo type and polyploidy might have played a role in accelerating shifts of diversification rates in Neotropical lineages. Future perspectives include formal subtribal classification, standardization of fossil calibration approaches and better links between diversification shifts and trait evolution

    Utilização da casca de coco como substrato agrícola.

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