Increased Stem Cell Factor Release by Hemangioma-Derived Endothelial Cells

Background: Capillary hemangiomas, the most common tumors in young children, consist of proliferating capillary vessels and endothelial cells. These tumors also contain large numbers of mast cells, compared with the normal surrounding skin or tissue. We have recently shown that stem cell factor (SCF), the gene product of the murine steel locus, can act as a chemoattractant for mast cells. In this study, we investigated whether SCF might be involved in the recruitment and maintenance of mast cells in hemangiomas. Experimental Design: Cultured endothelial cells derived from a murine hemangioma were compared with normal vascular endothelial cells for the ability to produce and release SCF, a mitogen for mast cells. Results: Conditioned medium from hemangioma-derived endothelial cells stimulated the proliferation of cultured mast cells. This proliferative activity was potentiated by interleukin-3. The same conditioned medium was unable to stimulate proliferation of mast cells expressing a defective receptor for SCF. The medium was also unable to stimulate proliferation when it was preincubated with neutralizing antibodies specific for SCF. Immunoprecipitation and Western blot analysis of the conditioned media from hemangioma cells and normal endothelial cells demonstrated the 31,000 molecular weight SCF in hemangioma-conditioned medium only. In addition, proliferative activity for mast cells could not be demonstrated in the conditioned medium of the normal endothelial cells, although Northern blot analysis indicated that both normal and hemangioma-derived endothelial cells express SCF mRNA. Reverse transcriptase-polymerase chain reaction techniques were used to amplify the DNA sequence coding for the proteolytic cleavage site used for release of SCF. Results indicated that both normal and hemangioma-derived endothelial cells express the same transcript for SCF. Conclusions: Our data suggest that increased release of SCF is a property of hemangioma-derived endothelial cells that may account for the high numbers of mast cells observed in hemangioma tissue. This increased release of SCF is not due to alternate splicing of SCF transcripts by hemangioma cells

A growth-rate indicator for Compton-thick active galactic nuclei

Due to their heavily obscured central engines, the growth rate of Compton-thick (CT) active galactic nuclei (AGN) is difficult to measure. A statistically significant correlation between the Eddington ratio, {\lambda}$_{Edd}$, and the X-ray power-law index, {\Gamma}, observed in unobscured AGN offers an estimate of their growth rate from X-ray spectroscopy (albeit with large scatter). However, since X-rays undergo reprocessing by Compton scattering and photoelectric absorption when the line-of-sight to the central engine is heavily obscured, the recovery of the intrinsic {\Gamma} is challenging. Here we study a sample of local, predominantly Compton-thick megamaser AGN, where the black hole mass, and thus Eddington luminosity, are well known. We compile results on X-ray spectral fitting of these sources with sensitive high-energy (E> 10 keV) NuSTAR data, where X-ray torus models which take into account the reprocessing effects have been used to recover the intrinsic {\Gamma} values and X-ray luminosities, L$_X$. With a simple bolometric correction to L$_X$ to calculate {\lambda}$_{Edd}$, we find a statistically significant correlation between {\Gamma} and {\lambda}$_{Edd}$ (p = 0.007). A linear fit to the data yields {\Gamma} = (0.41$\pm$0.18)log$_{10}${\lambda}$_{Edd}$+(2.38$\pm$ 0.20), which is statistically consistent with results for unobscured AGN. This result implies that torus modeling successfully recovers the intrinsic AGN parameters. Since the megamasers have low-mass black holes (M$_{BH}\approx10^6-10^7$ M$_{sol}$) and are highly inclined, our results extend the {\Gamma}-{\lambda}$_{Edd}$ relationship to lower masses and argue against strong orientation effects in the corona, in support of AGN unification. Finally this result supports the use of {\Gamma} as a growth-rate indicator for accreting black holes, even for Compton-thick AGN.Comment: Accepted for publication in Ap

Heavy X-ray obscuration in the most-luminous galaxies discovered by WISE

Hot Dust-Obscured Galaxies (Hot DOGs) are hyperluminous ($L_{\mathrm{8-1000\,\mu m}}>10^{13}\,\mathrm{L_\odot}$) infrared galaxies with extremely high (up to hundreds of K) dust temperatures. The sources powering both their extremely high luminosities and dust temperatures are thought to be deeply buried and rapidly accreting supermassive black holes (SMBHs). Hot DOGs could therefore represent a key evolutionary phase in which the SMBH growth peaks. X-ray observations can be used to study their obscuration levels and luminosities. In this work, we present the X-ray properties of the 20 most-luminous ($L_{\mathrm{bol}}\gtrsim10^{14}\, L_\odot$) known Hot DOGs at $z=2-4.6$. Five of them are covered by long-exposure ($10-70$ ks) Chandra and XMM-Newton observations, with three being X-ray detected, and we study their individual properties. One of these sources (W0116$-$0505) is a Compton-thick candidate, with column density $N_H=(1.0-1.5)\times10^{24}\,\mathrm{cm^{-2}}$ derived from X-ray spectral fitting. The remaining 15 Hot DOGs have been targeted by a Chandra snapshot (3.1 ks) survey. None of these 15 is individually detected; therefore we applied a stacking analysis to investigate their average emission. From hardness-ratio analysis, we constrained the average obscuring column density and intrinsic luminosity to be log$N_H\,\mathrm{[cm^{-2}]}>23.5$ and $L_X\gtrsim10^{44}\,\mathrm{erg\,cm^{-2}\,s^{-1}}$, which are consistent with results for individually detected sources. We also investigated the $L_X-L_{6\mu\mathrm{m}}$ and $L_X-L_{bol}$ relations, finding hints that Hot DOGs are typically X-ray weaker than expected, although larger samples of luminous obscured QSOs are needed to derive solid conclusions.Comment: MNRAS, accepted 2017 November 29 . Received 2017 November 29 ; in original form 2017 October 11. 15 pages, 6 figure

Review of "Proteins of the Cerebrospinal Fluid" (2(nd )Edition) by Edward J. Thompson

This book on cerebrospinal fluid (CSF) proteins is primarily focused on immunoglobulins. The book was written as an extension of a meeting on multiple sclerosis to provide a more extensive consideration of the CSF

Measuring the Obscuring Column of a Disk Megamaser AGN in a Nearby Merger

Active galactic nuclei (AGNs) hosting disk water megamasers are well known to be obscured by large amounts of gas, likely due to the presence along the line of sight of an almost edge-on disky structure orbiting the supermassive black hole. Correcting for the high obscuration is crucial to infer parameters intrinsic to the source, like its luminosity. We present a broadband X-ray spectral analysis of a water megamaser AGN in an early merger (NGC 5765B), combining Chandra and NuSTAR data. NGC 5765B is highly Compton-thick and reflection-dominated, following the general trend among disk megamasers. Combining the exquisite black hole mass from masers with our X-ray spectroscopy, the Eddington ratio of the megamaser is estimated to be in the 2%–14% range, and its robustness is confirmed through SED fitting

Investigating the evolution of the dual AGN system ESO~509-IG066

We analyze the evolution of the dual AGN in ESO 509-IG066, a galaxy pair located at z=0.034 whose nuclei are separated by 11 kpc. Previous observations with XMM-Newton on this dual AGN found evidence for two moderately obscured ($N_H\sim10^{22}$ cm$^{-2}$) X-ray luminous ($L_X\sim10^{43}$ erg/s) nuclear sources. We present an analysis of subsequent Chandra, NuSTAR and Swift/XRT observations that show one source has dropped in flux by a factor of 10 between 2004 and 2011, which could be explained by either an increase in the absorbing column or an intrinsic fading of the central engine possibly due to a decrease in mass accretion. Both of these scenarios are predicted by galaxy merger simulations. The source which has dropped in flux is not detected by NuSTAR, which argues against absorption, unless it is extreme. However, new Keck/LRIS optical spectroscopy reveals a previously unreported broad H-alpha line which is highly unlikely to be visible under the extreme absorption scenario. We therefore conclude that the black hole in this nucleus has undergone a dramatic drop in accretion rate. From AO-assisted near-infrared integral-field spectroscopy of the other nucleus, we find evidence that the galaxy merger is having a direct effect on the kinematics of the gas close to the nucleus of the galaxy, providing a direct observational link between the galaxy merger and the mass accretion rate on to the black hole.Comment: Accepted for publication in Ap

X-ray bolometric corrections for Compton-thick active galactic nuclei

We present X-ray bolometric correction factors, $\kappa_{Bol}$ ($\equiv L_{Bol}/L_X$), for Compton-thick (CT) active galactic nuclei (AGN) with the aim of testing AGN torus models, probing orientation effects, and estimating the bolometric output of the most obscured AGN. We adopt bolometric luminosities, $L_{Bol}$, from literature infrared (IR) torus modeling and compile published intrinsic 2--10 keV X-ray luminosities, $L_{X}$, from X-ray torus modeling of NuSTAR data. Our sample consists of 10 local CT AGN where both of these estimates are available. We test for systematic differences in $\kappa_{Bol}$ values produced when using two widely used IR torus models and two widely used X-ray torus models, finding consistency within the uncertainties. We find that the mean $\kappa_{Bol}$ of our sample in the range $L_{Bol}\approx10^{42}-10^{45}$ erg/s is log$_{10}\kappa_{Bol}=1.44\pm0.12$ with an intrinsic scatter of $\sim0.2$ dex, and that our derived $\kappa_{Bol}$ values are consistent with previously established relationships between $\kappa_{Bol}$ and $L_{Bol}$ and $\kappa_{Bol}$ and Eddington ratio. We investigate if $\kappa_{Bol}$ is dependent on $N_H$ by comparing our results on CT AGN to published results on less-obscured AGN, finding no significant dependence. Since many of our sample are megamaser AGN, known to be viewed edge-on, and furthermore under the assumptions of AGN unification whereby unobscured AGN are viewed face-on, our result implies that the X-ray emitting corona is not strongly anisotropic. Finally, we present $\kappa_{Bol}$ values for CT AGN identified in X-ray surveys as a function of their observed $L_X$, where an estimate of their intrinsic $L_{X}$ is not available, and redshift, useful for estimating the bolometric output of the most obscured AGN across cosmic time.Comment: Accepted for publication in Ap

Broadband Observations of the Compton-thick Nucleus of NGC 3393

We present new NuSTAR and Chandra observations of NGC 3393, a galaxy reported to host the smallest separation dual AGN resolved in the X-rays. While past results suggested a 150 pc separation dual AGN, three times deeper Chandra imaging, combined with adaptive optics and radio imaging suggest a single, heavily obscured, radio-bright AGN. Using VLA and VLBA data, we find an AGN with a two-sided jet rather than a dual AGN and that the hard X-ray, UV, optical, NIR, and radio emission are all from a single point source with a radius <0.2". We find that the previously reported dual AGN is most likely a spurious detection resulting from the low number of X-ray counts (<160) at 6-7 keV and Gaussian smoothing of the data on scales much smaller than the PSF (0.25" vs. 0.80" FWHM). We show that statistical noise in a single Chandra PSF generates spurious dual peaks of the same separation (0.55$\pm$0.07" vs. 0.6") and flux ratio (39$\pm$9% vs. 32% of counts) as the purported dual AGN. With NuSTAR, we measure a Compton-thick source (NH=$2.2\pm0.4\times10^{24}$ cm$^{-2}$) with a large torus half-opening angle, {\theta}=79 which we postulate results from feedback from strong radio jets. This AGN shows a 2-10 keV intrinsic to observed flux ratio of 150. Using simulations, we find that even the deepest Chandra observations would severely underestimate the intrinsic luminosity of NGC 3393 above z>0.2, but would detect an unobscured AGN of this luminosity out to high redshift (z=5).Comment: Accepted for publication in ApJ. 15 Figures and 4 table

Naval Services Game \u2713

Conducted from 27-31 January 2014 at the United States Naval war College in Newport, Rhode Island, the Naval Services Game brought together 30 members of the Navy and Marine Corps for the Navy and Marine Corps for the purpose of exploring U.S. Navy and U.S. Marine Corps integration, The intent of this integration is to develop forward deployed naval forces with integrated capabilities for engagement and crisis responses. Participants were assigned to three, independent (Blue) player cells. These cells were tasked similarly with players providing a perspective of the service component commander in theater initially, then the Service Chief