Progettazione e monitoraggio di una campagna di web marketing: il caso Thetis S.r.l.

In questo elaborato, dopo una descrizione del Web Marketing, degli strumenti utilizzati in questa disciplina e dell’azienda che mi ha ospitato durante il tirocinio, vengono illustrate le attività progettate in ottica P-D-C-A per l’ideazione, progettazione e monitoraggio di una campagna di Web Marketing che ha avuto l’obiettivo di portare traffico qualificato verso il sito www.thejambo.it. Per la fase di panificazione, (Plan), sono stati analizzati gli aspetti fondamentali della campagna in modo da redigere un piano di web marketing contenente gli obiettivi, il target di riferimento, gli strumenti da utilizzare sia per le attività di configurazione sia per quelle di monitoraggio, la pianificazione temporale e il budget disponibile per le varie azioni di promozione. Nella fase di esecuzione, (Do), sono state svolte le attività di configurazione delle campagne PayPerClick su Google, You Tube e Facebook e di implementazione del sistema di monitoraggio. Dopo l’attivazione delle campagne, sono state eseguite le azioni di monitoraggio e di ottimizzazione (fasi di Check e di Act) in modo da massimizzare le performance ed effettuare, ove necessario, azioni correttive. Nell'ultima parte della tesi, vengono esposti e analizzati i risultati conseguiti dalle campagne, le considerazioni sul loro andamento e i punti di forza e di debolezza riscontrati. This thesis, after a description including the concept of Web Marketing along with its tools and the company where I trained, shows the different steps, designed in accordance with P-D-C-A model, of a Web Marketing campaign, whose aim was to bring qualified traffic to the site www.thejambo.it. For the planning phase, (Plan), fundamental sides of the campaign were analyzed, so to structure a web marketing plan, including the goals, the target range, the tools to configure the campaign and the monitoring system, the temporal planning and the budget for different advertising actions. In the execution step, (Do), the necessary activities to configure the PayPerClick campaigns on Google, on YouTube and on Facebook, and the monitoring system were presented. After starting up the campaigns, monitoring and optimization actions were executed (Check and Act phases) to maximize their results and, if necessary, to realize corrective actions. The last section of the thesis focused on the results of campaigns, on the observations of their trend and on strength and weakness points encountered

Circ-ZNF609 Is a Circular RNA that Can Be Translated and Functions in Myogenesis

Circular RNAs (circRNAs) constitute a family of transcripts with unique structures and still largely unknown functions. Their biogenesis, which proceeds via a back-splicing reaction, is fairly well characterized, whereas their role in the modulation of physiologically relevant processes is still unclear. Here we performed expression profiling of circRNAs during in vitro differentiation of murine and human myoblasts, and we identified conserved species regulated in myogenesis and altered in Duchenne muscular dystrophy. A high-content functional genomic screen allowed the study of their functional role in muscle differentiation. One of them, circ-ZNF609, resulted in specifically controlling myoblast proliferation. Circ-ZNF609 contains an open reading frame spanning from the start codon, in common with the linear transcript, and terminating at an in-frame STOP codon, created upon circularization. Circ-ZNF609 is associated with heavy polysomes, and it is translated into a protein in a splicing-dependent and cap-independent manner, providing an example of a protein-coding circRNA in eukaryotes

Tailored star poly (ε-caprolactone) wet-spun scaffolds for in vivo regeneration of long bone critical size defects

One of the most challenging requirements of a successful bone tissue engineering approach is the development of scaffolds specifically tailored to individual tissue defects. Besides materials chemistry, well-defined scaffold’s structural features at the micro- and macro-levels are needed for optimal bone in-growth. In this study, polymeric fibrous scaffolds with a controlled internal network of pores and modelled on the anatomical shape and dimensions of a critical size bone defect in a rabbit’s radius model were developed by employing a computer-aided wet-spinning technique. The tailored scaffolds made of star poly(ε caprolactone) or star poly(ε-caprolactone)– hydroxyapatite composite material were implanted into 20-mm segmental defects created in radial diaphysis of New Zealand white rabbits. Bone regeneration and tissue response were assessed by X-rays and histological analysis at 4, 8 and 12 weeks after surgery. No signs of macroscopic and microscopic inflammatory reactions were detected, and the developed scaffolds showed a good ability to support and promote the bone regeneration process. However, no significant differences in osteoconductivity were observed between star poly(ε-caprolactone) and star poly(ε-caprolactone)–hydroxyapatite scaffolds. Long-term study on implanted star poly(ε-caprolactone) scaffolds confirmed the presence of signs of bone regeneration and remodelling, particularly evident at 24 weeks

Bimodal CD40/Fas-Dependent Crosstalk between iNKT Cells and Tumor-Associated Macrophages Impairs Prostate Cancer Progression

Heterotypic cellular and molecular interactions in the tumor microenvironment (TME) control cancer progression. Here, we show that CD1d-restricted invariant natural killer (iNKT) cells control prostate cancer (PCa) progression by sculpting the TME. In a mouse PCa model, iNKT cells restrained the proangiogenic and immunosuppressive capabilities of tumor-infiltrating immune cells by reducing proangiogenic TIE2+, M2-like macrophages (TEMs), and sustaining pro-inflammatory M1-like macrophages. iNKT cells directly contacted macrophages in the PCa stroma, and iNKT cell transfer into tumorbearing mice abated TEMs, delaying tumor progression. iNKT cells modulated macrophages through the cooperative engagement of CD1d, Fas, and CD40, which promoted selective killing of M2-like and survival of M1-like macrophages. Human PCa aggressiveness associate with reduced intra-tumoral iNKT cells, increased TEMs, and expression of pro-angiogenic genes, underscoring the clinical significance of this crosstalk. Therefore, iNKT cells may control PCa through mechanisms involving differential macrophage modulation, which may be harnessed for therapeutically reprogramming the TME

Lack of SARS-CoV-2 RNA environmental contamination in a tertiary referral hospital for infectious diseases in Northern Italy

none140noNAnoneColaneri M.; Seminari E.; Piralla A.; Zuccaro V.; Di Filippo A.; Baldanti F.; Bruno R.; Mondelli M.U.; Brunetti E.; Di Matteo A.; Maiocchi L.; Pagnucco L.; Mariani B.; Ludovisi S.; Lissandrin R.; Parisi A.; Sacchi P.; Patruno S.F.A.; Michelone G.; Gulminetti R.; Zanaboni D.; Novati S.; Maserati R.; Orsolini P.; Vecchia M.; Sciarra M.; Asperges E.; Sambo M.; Biscarini S.; Lupi M.; Roda S.; Chiara Pieri T.; Gallazzi I.; Sachs M.; Valsecchi P.; Perlini S.; Alfano C.; Bonzano M.; Briganti F.; Crescenzi G.; Giulia Falchi A.; Guarnone R.; Guglielmana B.; Maggi E.; Martino I.; Pettenazza P.; Pioli di Marco S.; Quaglia F.; Sabena A.; Salinaro F.; Speciale F.; Zunino I.; De Lorenzo M.; Secco G.; Dimitry L.; Cappa G.; Maisak I.; Chiodi B.; Sciarrini M.; Barcella B.; Resta F.; Moroni L.; Vezzoni G.; Scattaglia L.; Boscolo E.; Zattera C.; Michele Fidel T.; Vincenzo C.; Vignaroli D.; Bazzini M.; Iotti G.; Mojoli F.; Belliato M.; Perotti L.; Mongodi S.; Tavazzi G.; Marseglia G.; Licari A.; Brambilla I.; Daniela B.; Antonella B.; Patrizia C.; Giulia C.; Giuditta C.; Marta C.; Rossana D.; Milena F.; Bianca M.; Roberta M.; Enza M.; Stefania P.; Maurizio P.; Elena P.; Antonio P.; Francesca R.; Antonella S.; Maurizio Z.; Guy A.; Laura B.; Ermanna C.; Giuliana C.; Luca D.; Gabriella F.; Gabriella G.; Alessia G.; Viviana L.; Claudia L.; Valentina M.; Simona P.; Marta P.; Alice B.; Giacomo C.; Irene C.; Alfonso C.; Di Martino R.; Di Napoli A.; Alessandro F.; Guglielmo F.; Loretta F.; Federica G.; Alessandra M.; Federica N.; Giacomo R.; Beatrice R.; Maria S.I.; Monica T.; Nepita Edoardo V.; Calvi M.; Tizzoni M.; Nicora C.; Triarico A.; Petronella V.; Marena C.; Muzzi A.; Lago P.; Comandatore F.; Bissignandi G.; Gaiarsa S.; Rettani M.; Bandi C.Colaneri, M.; Seminari, E.; Piralla, A.; Zuccaro, V.; Di Filippo, A.; Baldanti, F.; Bruno, R.; Mondelli, M. U.; Brunetti, E.; Di Matteo, A.; Maiocchi, L.; Pagnucco, L.; Mariani, B.; Ludovisi, S.; Lissandrin, R.; Parisi, A.; Sacchi, P.; Patruno, S. F. A.; Michelone, G.; Gulminetti, R.; Zanaboni, D.; Novati, S.; Maserati, R.; Orsolini, P.; Vecchia, M.; Sciarra, M.; Asperges, E.; Sambo, M.; Biscarini, S.; Lupi, M.; Roda, S.; Chiara Pieri, T.; Gallazzi, I.; Sachs, M.; Valsecchi, P.; Perlini, S.; Alfano, C.; Bonzano, M.; Briganti, F.; Crescenzi, G.; Giulia Falchi, A.; Guarnone, R.; Guglielmana, B.; Maggi, E.; Martino, I.; Pettenazza, P.; Pioli di Marco, S.; Quaglia, F.; Sabena, A.; Salinaro, F.; Speciale, F.; Zunino, I.; De Lorenzo, M.; Secco, G.; Dimitry, L.; Cappa, G.; Maisak, I.; Chiodi, B.; Sciarrini, M.; Barcella, B.; Resta, F.; Moroni, L.; Vezzoni, G.; Scattaglia, L.; Boscolo, E.; Zattera, C.; Michele Fidel, T.; Vincenzo, C.; Vignaroli, D.; Bazzini, M.; Iotti, G.; Mojoli, F.; Belliato, M.; Perotti, L.; Mongodi, S.; Tavazzi, G.; Marseglia, G.; Licari, A.; Brambilla, I.; Daniela, B.; Antonella, B.; Patrizia, C.; Giulia, C.; Giuditta, C.; Marta, C.; D'Alterio, Rossana; Milena, F.; Bianca, M.; Roberta, M.; Enza, M.; Stefania, P.; Maurizio, P.; Elena, P.; Antonio, P.; Francesca, R.; Antonella, S.; Maurizio, Z.; Guy, A.; Laura, B.; Ermanna, C.; Giuliana, C.; Luca, D.; Gabriella, F.; Gabriella, G.; Alessia, G.; Viviana, L.; Meisina, Claudia; Valentina, M.; Simona, P.; Marta, P.; Alice, B.; Giacomo, C.; Irene, C.; Alfonso, C.; Di Martino, R.; Di Napoli, A.; Alessandro, F.; Guglielmo, F.; Loretta, F.; Federica, G.; Albertini, Alessandra; Federica, N.; Giacomo, R.; Beatrice, R.; Maria, S. I.; Monica, T.; Nepita Edoardo, V.; Calvi, M.; Tizzoni, M.; Nicora, C.; Triarico, A.; Petronella, V.; Marena, C.; Muzzi, A.; Lago, P.; Comandatore, F.; Bissignandi, G.; Gaiarsa, S.; Rettani, M.; Bandi, C

Clinical characteristics of coronavirus disease (COVID-19) early findings from a teaching hospital in Pavia, North Italy, 21 to 28 February 2020

We describe clinical characteristics, treatments and outcomes of 44 Caucasian patients with coronavirus disease (COVID-19) at a single hospital in Pavia, Italy, from 21\u201328 February 2020, at the beginning of the outbreak in Europe. Seventeen patients developed severe disease, two died. After a median of 6 days, 14 patients were discharged from hospital. Predictors of lower odds of discharge were age>65 years, antiviral treatment and for severe disease, lactate dehydrogenase >300 mg/dL

Bronchoalveolar lavage fluid cytological findings in healthy Amiata donkeys

Background: The approach to respiratory diseases in donkeys is similar to that for horses, nevertheless bronchoalveolar fluid (BALF) and tracheal wash (TW) cytology in this species have been described only a few times in the literature. Aim: To describe BALF cytological findings in a cohort of 24 healthy Amiata donkeys. Methods: Bronchoalveolar lavage (BAL) was performed under standing sedation with a large animal where the broncho-alveolar lavage catheter passed blindly through the nasal passage into the trachea. Results: The total nucleated cell count (TNCC) of the BALF was found similar to that already described in healthy horses and donkeys. No differences in the differential count were observed according to age and sex. A decreased macrophage percentage and an increased eosinophil percentage was observed in our donkey population when compared to the existing reference range for horses. Conclusions: The reference intervals for BAL cytology in donkeys may be significantly different for those referred for horses

pHyloGASTRO in the Treatment of Equine Gastric Ulcer Lesions

Equine gastric ulcer syndrome (EGUS) is the most common disease of the equine stomach with high prevalence of both squamous and glandular disease reported in various populations. The aim of this study was to evaluate the effectiveness of a phytotherapic compound (pHhyloGASTRO) in the therapy of EGUS. The study was performed as a randomized double-blinded single-center study. The study population was composed of 19 equids which were submitted to gastroscopy before and after a 6-week treatment with feed additive (10/19) (pHyloGASTRO, 4Union B.I.O. srl, Italy) or a placebo (9/19). Severity grade was evaluated on a scale from 0 to 4. The variables of interest were gastric lesion score and improvement grade. Changes and comparisons of variables were performed by contingency table analyses. P level of significance was set at .05 in all analyses. In terms of gastric lesion scores, the treated group improved significantly compared to the placebo group. pHyloGASTRO seems to be effective in the treatment of EGUS. Further studies are needed to verify whether prolonged administration of pHyloGASTRO could be more effective in completely healing gastric lesions

La storia dell'arte. 4. Dal Barocco all'Art Nouveau

none5Testo di storia dell’arte moderna e contemporanea che narra l’evoluzione delle forme e delle idee artistiche dall’età barocca al primo Novecento attraverso la riproduzione iconografica, la descrizione e l’intepretazione dei capolavori più celebri. Il volume è arricchito da sezioni di approfondimento relative alle tecniche, alle influenze e trasmissioni di canoni e stili, e alle teorie estetiche essenziali a meglio conoscere e comprendere l’arte del periodo in esame.noneBertelli C.; Briganti B.; di Robilant M.; Filippi F.; Gatti A.Bertelli, C.; Briganti, B.; di Robilant, M.; Filippi, F.; Gatti, Andre

Mieloencefalopatia degenerativa equina: sintomatologia clinica, indagini collaterali ed esame post-mortem in una puledra trottatrice

The aim of the present paper was to describe a case of Equine Degenerative Myeloencephalopa- thy (EDM) in a female yearling trotter horse with locomotory and postural deficits on the four limbs. Cranial nerve assessment was normal and the horse was bright and alert. On neurological examination, wide-based stance, grade 4/5 sym- metrical ataxia, grade 2/5 paresis in thoracic limbs and 3/5 in pelvic limbs were detected. Mental sta- tus, cranial nerves and perineal reflex were nor- mal, while cervico-facial reflex was absent. Neu- roanatomical localization suggested a cervico-tho- racic spinal cord lesion. Plasmatic vitamin E con- centration was low, plain lateral radiography and myelography of the caudal skull and cervical ver- tebrae was unremarkable. Cerebrospinal fluid (CSF) was clear and colourless. Total protein con- centration and total nucleated cell count were wi- thin normal references. On the basis of the clini- cal and diagnostic findings, EDM was considered most likely. Vitamin E was administered per os at the dose of 6000 IU/foal with mild improvement. The filly was euthanized six months later. No ma- croscopic lesions were observed in the brain and spinal cord. At different levels of the spinal cord, histological lesions were characterized by astro- gliosis, loss of myelinated fibres, and accumulation of lipofuscin granules