1,092 research outputs found
A matrix isolation and computational study of molecular palladium fluorides : does PdF₆ exist?
Palladium atoms generated by thermal evaporation and laser ablation were reacted with and trapped in F₂ /Ar, F₂ /Ne, and neat F₂ matrices. The products were characterized by electronic absorption and infrared spectroscopy, together with relativistic density functional theory calculations as well as coupled cluster calculations. Vibrational modes at 540 and 617 cm⁻¹ in argon matrices were assigned to molecular PdF and PdF₂ , and a band at 692 cm⁻¹ was assigned to molecular PdF₄ . A band at 624 cm⁻¹ can be assigned to either PdF₃ or PdF₆, with the former preferred from experimental considerations. Although calculations might support the latter assignment, our conclusion is that in these detailed experiments there is no convincing evidence for PdF₆
Exogenous spatial precuing reliably modulates object processing but not object substitution masking
Object substitution masking (OSM) is used in behavioral and imaging studies to investigate processes associated with the formation of a conscious percept. Reportedly, OSM occurs only when visual attention is diffusely spread over a search display or focused away from the target location. Indeed, the presumed role of spatial attention is central to theoretical accounts of OSM and of visual processing more generally (Di Lollo, Enns, & Rensink, Journal of Experimental Psychology: General 129:481–507, 2000). We report a series of five experiments in which valid spatial precuing is shown to enhance the ability of participants to accurately report a target but, in most cases, without affecting OSM. In only one experiment (Experiment 5) was a significant effect of precuing observed on masking. This is in contrast to the reliable effect shown across all five experiments in which precuing improved overall performance. The results are convergent with recent findings from Argyropoulos, Gellatly, and Pilling (Journal of Experimental Psychology: Human Perception and Performance 39:646–661, 2013), which show that OSM is independent of the number of distractor items in a display. Our results demonstrate that OSM can operate independently of focal attention. Previous claims of the strong interrelationship between OSM and spatial attention are likely to have arisen from ceiling or floor artifacts that restricted measurable performance
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Biases in the perceived timing of perisaccadic perceptual and motor events
Subjects typically experience the temporal interval immediately following a saccade as longer than a comparable control interval. One explanation of this effect is that the brain antedates the perceptual onset of a saccade target to around the time of saccade initiation. This could explain the apparent continuity of visual perception across eye movements. Thisantedating account was tested in three experiments in which subjects made saccades of differing extents and then judged either the duration or the temporal order of key events. Postsaccadic stimuli underwent subjective temporal lengthening and had early perceived onsets. A temporally advanced awareness of saccade completion was also found, independently of antedating effects. These results provide convergent evidence supporting antedating and differentiating it from other temporal biases
Using interactive lecture demonstrations to invigorate chemistry lectures
Demonstrations are commonly used in Chemistry lectures to illustrate chemical phenomena and as entertainment. Interactive lecture demonstrations have been introduced into first year undergraduate classes which more deliberately connect the demonstration with the course content. Through the development of worksheets, students are actively engaged with predicting, observing and explaining the results of the demonstration. Evaluation of this approach through surveys and class observation suggest that students found it engaging and effective for their learning
CD3ζ-based chimeric antigen receptors mediate T cell activation viacis- andtrans-signalling mechanisms: implications for optimization of receptor structure for adoptive cell therapy
Chimeric antigen receptors (CARs) can mediate redirected lysis of tumour cells in a major histocompatibility complex (MHC)-independent manner, thereby enabling autologous adoptive T cell therapy for a variety of malignant neoplasms. Currently, most CARs incorporate the T cell receptor (TCR) CD3ζ signalling chain; however, the precise mechanisms responsible for CAR-mediated T cell activation are unclear. In this study, we used a series of immunoreceptor tyrosine-based activation motif (ITAM)-mutant and transmembrane-modified receptors to demonstrate that CARs activate T cells both directly via the antigen-ligated signalling chain and indirectly via associated chains within the TCR complex. These observations allowed us to generate new receptors capable of eliciting polyfunctional responses in primary human T cells. This work increases our understanding of CAR function and identifies new avenues for the optimization of CAR-based therapeutic interventions
Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination
Antigen recognition by the T-cell receptor (TCR) is a hallmark of the adaptive immune system. When the TCR engages a peptide bound to the restricting major histocompatibility complex molecule (pMHC), it transmits a signal via the associated CD3 complex. How the extracellular antigen recognition event leads to intracellular phosphorylation remains unclear. Here, we used single-molecule localization microscopy to quantify the organization of TCR–CD3 complexes into nanoscale clusters and to distinguish between triggered and nontriggered TCR–CD3 complexes. We found that only TCR–CD3 complexes in dense clusters were phosphorylated and associated with downstream signaling proteins, demonstrating that the molecular density within clusters dictates signal initiation. Moreover, both pMHC dose and TCR–pMHC affinity determined the density of TCR–CD3 clusters, which scaled with overall phosphorylation levels. Thus, TCR–CD3 clustering translates antigen recognition by the TCR into signal initiation by the CD3 complex, and the formation of dense signaling-competent clusters is a process of antigen discrimination
Oxidative Stress in Cells with Extra Centrosomes Drives Non-Cell-Autonomous Invasion
This work was supported by a Cancer Research UK Centre Grant to Barts Cancer Institute (C16420/A18066). P.R.C. was funded by BBSRC (BB/M006174/1) and Barts and The London Charity (297/2249). S.A.G. is a fellow of the Lister Institute and is supported by the Medical Research Council (MR/M010414/1)
Low levels of fruit nitrogen as drivers for the evolution of Madagascar's primate communities
The uneven representation of frugivorous mammals and birds across tropical regions – high in the New World, low in Madagascar and intermediate in Africa and Asia – represents a long-standing enigma in ecology. Several hypotheses have been proposed to explain these differences but the ultimate drivers remain unclear. Here, we tested the hypothesis that fruits in Madagascar contain insufficient nitrogen to meet primate metabolic requirements, thus constraining the evolution of frugivory. We performed a global analysis of nitrogen in fruits consumed by primates, as collated from 79 studies. Our results showed that average frugivory among lemur communities was lower compared to New World and Asian-African primate communities. Fruits in Madagascar contain lower average nitrogen than those in the New World and Old World. Nitrogen content in the overall diets of primate species did not differ significantly between major taxonomic radiations. There is no relationship between fruit protein and the degree of frugivory among primates either globally or within regions, with the exception of Madagascar. This suggests that low protein availability in fruits influences current lemur communities to select for protein from other sources, whereas in the New World and Old World other factors are more significant in shaping primate communities
Valence Fluctuations Revealed by Magnetic Field Scan: Comparison with Experiments in YbXCu_4 (X=In, Ag, Cd) and CeYIn_5 (Y=Ir, Rh)
The mechanism of how critical end points of the first-order valence
transitions (FOVT) are controlled by a magnetic field is discussed. We
demonstrate that the critical temperature is suppressed to be a quantum
critical point (QCP) by a magnetic field. This results explain the field
dependence of the isostructural FOVT observed in Ce metal and YbInCu_4.
Magnetic field scan can lead to reenter in a critical valence fluctuation
region. Even in the intermediate-valence materials, the QCP is induced by
applying a magnetic field, at which the magnetic susceptibility also diverges.
The driving force of the field-induced QCP is shown to be a cooperative
phenomenon of the Zeeman effect and the Kondo effect, which creates a distinct
energy scale from the Kondo temperature. The key concept is that the closeness
to the QCP of the FOVT is capital in understanding Ce- and Yb-based heavy
fermions. It explains the peculiar magnetic and transport responses in CeYIn_5
(Y=Ir, Rh) and metamagnetic transition in YbXCu_4 for X=In as well as the sharp
contrast between X=Ag and Cd.Comment: 14 pages, 9 figures, OPEN SELECT in J. Phys. Soc. Jp
Observation of charge-dependent azimuthal correlations and possible local strong parity violation in heavy ion collisions
Parity-odd domains, corresponding to non-trivial topological solutions of the
QCD vacuum, might be created during relativistic heavy-ion collisions. These
domains are predicted to lead to charge separation of quarks along the orbital
momentum of the system created in non-central collisions. To study this effect,
we investigate a three particle mixed harmonics azimuthal correlator which is a
\P-even observable, but directly sensitive to the charge separation effect. We
report measurements of this observable using the STAR detector in Au+Au and
Cu+Cu collisions at =200 and 62~GeV. The results are presented
as a function of collision centrality, particle separation in rapidity, and
particle transverse momentum. A signal consistent with several of the
theoretical expectations is detected in all four data sets. We compare our
results to the predictions of existing event generators, and discuss in detail
possible contributions from other effects that are not related to parity
violation.Comment: 17 pages, 14 figures, as accepted for publication in Physical Review
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