Kiloparsec-scale Spatial Offsets in Double-peaked Narrow-line Active Galactic Nuclei. I. Markers for Selection of Compelling Dual Active Galactic Nucleus Candidates

Merger-remnant galaxies with kpc-scale separation dual active galactic nuclei (AGNs) should be widespread as a consequence of galaxy mergers and triggered gas accretion onto supermassive black holes, yet very few dual AGNs have been observed. Galaxies with double-peaked narrow AGN emission lines in the Sloan Digital Sky Survey are plausible dual AGN candidates, but their double-peaked profiles could also be the result of gas kinematics or AGN-driven outflows and jets on small or large scales. To help distinguish between these scenarios, we have obtained spatial profiles of the AGN emission via follow-up long-slit spectroscopy of 81 double-peaked narrow-line AGNs in SDSS at 0.03 < z < 0.36 using Lick, Palomar, and MMT Observatories. We find that all 81 systems exhibit double AGN emission components with ~kpc projected spatial separations on the sky, which suggests that they are produced by kpc-scale dual AGNs or kpc-scale outflows, jets, or rotating gaseous disks. In addition, we find that the subsample (58%) of the objects with spatially compact emission components may be preferentially produced by dual AGNs, while the subsample (42%) with spatially extended emission components may be preferentially produced by AGN outflows. We also find that for 32% of the sample the two AGN emission components are preferentially aligned with the host galaxy major axis, as expected for dual AGNs orbiting in the host galaxy potential. Our results both narrow the list of possible physical mechanisms producing the double AGN components, and suggest several observational criteria for selecting the most promising dual AGN candidates from the full sample of double-peaked narrow-line AGNs. Using these criteria, we determine the 17 most compelling dual AGN candidates in our sample.Comment: 12 pages, 8 figures, published in ApJ. Modified from original version to reflect referee's comment

WISE J233237.05–505643.5: A Double-peaked, Broad-lined Active Galactic Nucleus with a Spiral-shaped Radio Morphology

We present radio continuum mapping, optical imaging, and spectroscopy of the newly discovered double-peaked, broad-lined active galactic nucleus (AGN) WISE J233237.05–505643.5 at redshift z = 0.3447. This source exhibits an FR-I and FR-II hybrid morphology, characterized by a bright core, jet, and Doppler-boosted lobe structures in Australian Telescope Compact Array continuum maps at 1.5, 5.6, and 9 GHz. Unlike most FR-II objects, W2332–5056 is hosted by a disk-like galaxy. The core has a projected 5'' linear radio feature that is perpendicular to the curved primary jet, hinting at unusual and complex activity within the inner 25 kpc. The multi-epoch, optical-near-IR photometric measurements indicate significant variability over a 3-20 yr baseline from the AGN component. Gemini South optical data show unusual double-peaked emission-line features: the centroids of the broad-lined components of Hα and Hβ are blueshifted with respect to the narrow lines and host galaxy by ~3800 km s^(–1). We examine possible cases that involve single or double supermassive black holes in the system and discuss the required future investigations to disentangle the mysterious nature of this system

Testing and Validation of High Density Resequencing Microarray for Broad Range Biothreat Agents Detection

Rapid and effective detection and identification of emerging microbiological threats and potential biowarfare agents is very challenging when using traditional culture-based methods. Contemporary molecular techniques, relying upon reverse transcription and/or polymerase chain reaction (RT-PCR/PCR) provide a rapid and effective alternative, however, such assays are generally designed and optimized to detect only a limited number of targets, and seldom are capable of differentiation among variants of detected targets. To meet these challenges, we have designed a broad-range resequencing pathogen microarray (RPM) for detection of tropical and emerging infectious agents (TEI) including biothreat agents: RPM-TEI v 1.0 (RPM-TEI). The scope of the RPM-TEI assay enables detection and differential identification of 84 types of pathogens and 13 toxin genes, including most of the class A, B and C select agents as defined by the Centers for Disease Control and Prevention (CDC, Atlanta, GA). Due to the high risks associated with handling these particular target pathogens, the sensitivity validation of the RPM-TEI has been performed using an innovative approach, in which synthetic DNA fragments are used as templates for testing the assay's limit of detection (LOD). Assay specificity and sensitivity was subsequently confirmed by testing with full-length genomic nucleic acids of selected agents. The LOD for a majority of the agents detected by RPM-TEI was determined to be at least 104 copies per test. Our results also show that the RPM-TEI assay not only detects and identifies agents, but is also able to differentiate near neighbors of the same agent types, such as closely related strains of filoviruses of the Ebola Zaire group, or the Machupo and Lassa arenaviruses. Furthermore, each RPM-TEI assay results in specimen-specific agent gene sequence information that can be used to assess pathogenicity, mutations, and virulence markers, results that are not generally available from multiplexed RT-PCR/PCR-based detection assays

Leapfrog diagnostics: Demonstration of a broad spectrum pathogen identification platform in a resource-limited setting

Background Resource-limited tropical countries are home to numerous infectious pathogens of both human and zoonotic origin. A capability for early detection to allow rapid outbreak containment and prevent spread to non-endemic regions is severely impaired by inadequate diagnostic laboratory capacity, the absence of a “cold chain” and the lack of highly trained personnel. Building up detection capacity in these countries by direct replication of the systems existing in developed countries is not a feasible approach and instead requires “leapfrogging” to the deployment of the newest diagnostic systems that do not have the infrastructure requirements of systems used in developed countries. Methods A laboratory for molecular diagnostics of infectious agents was established in Bo, Sierra Leone with a hybrid solar/diesel/battery system to ensure stable power supply and a satellite modem to enable efficient communication. An array of room temperature stabilization and refrigeration technologies for reliable transport and storage of reagents and biological samples were also tested to ensure sustainable laboratory supplies for diagnostic assays. Results The laboratory demonstrated its operational proficiency by conducting an investigation of a suspected avian influenza outbreak at a commercial poultry farm at Bo using broad range resequencing microarrays and real time RT-PCR. The results of the investigation excluded influenza viruses as a possible cause of the outbreak and indicated a link between the outbreak and the presence of Klebsiella pneumoniae. Conclusions This study demonstrated that by application of a carefully selected set of technologies and sufficient personnel training, it is feasible to deploy and effectively use a broad-range infectious pathogen detection technology in a severely resource-limited setting

Simulated patient programmes in Europe:collegiality or separate development

Aims: In order for different SP programmes to learn from each other, there needs to be some basis for establishing meaningful comparisons. Method: In 2006, the Association of Standardized Patient Educators (ASPE) piloted a survey instrument that would facilitate comparisons of SP educational practices in different institutions. Four European countries at varying stages of SP programme development were selected as representative of the spread of SP experience in Europe (Belgium, Ireland, Scotland and the Netherlands). Key SP contacts were identified in each medical school. Contacts were asked to complete a 49-item questionnaire developed collaboratively between ASPE and the authors. The overall response rate was 86%. Results: There were considerable differences between countries in terms of their approach to developing SPs and quality assuring their performance. Whilst SP education was regarded as an expensive enterprise, there was little evidence of resource sharing between different centres in the same country. Conclusions: There is a clear need to facilitate closer collaboration between centres in developing and quality assuring SPs

Molecular Characterization of Multidrug Resistant Hospital Isolates Using the Antimicrobial Resistance Determinant Microarray

<div><p>Molecular methods that enable the detection of antimicrobial resistance determinants are critical surveillance tools that are necessary to aid in curbing the spread of antibiotic resistance. In this study, we describe the use of the Antimicrobial Resistance Determinant Microarray (ARDM) that targets 239 unique genes that confer resistance to 12 classes of antimicrobial compounds, quaternary amines and streptothricin for the determination of multidrug resistance (MDR) gene profiles. Fourteen reference MDR strains, which either were genome, sequenced or possessed well characterized drug resistance profiles were used to optimize detection algorithms and threshold criteria to ensure the microarray's effectiveness for unbiased characterization of antimicrobial resistance determinants in MDR strains. The subsequent testing of <i>Acinetobacter baumannii</i>, <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> hospital isolates revealed the presence of several antibiotic resistance genes [e.g. belonging to TEM, SHV, OXA and CTX-M classes (and OXA and CTX-M subfamilies) of β-lactamases] and their assemblages which were confirmed by PCR and DNA sequence analysis. When combined with results from the reference strains, ∼25% of the ARDM content was confirmed as effective for representing allelic content from both Gram-positive and –negative species. Taken together, the ARDM identified MDR assemblages containing six to 18 unique resistance genes in each strain tested, demonstrating its utility as a powerful tool for molecular epidemiological investigations of antimicrobial resistance in clinically relevant bacterial pathogens.</p></div

Leapfrog diagnostics: Demonstration of a broad spectrum pathogen identification platform in a resource-limited setting

Abstract Background Resource-limited tropical countries are home to numerous infectious pathogens of both human and zoonotic origin. A capability for early detection to allow rapid outbreak containment and prevent spread to non-endemic regions is severely impaired by inadequate diagnostic laboratory capacity, the absence of a “cold chain” and the lack of highly trained personnel. Building up detection capacity in these countries by direct replication of the systems existing in developed countries is not a feasible approach and instead requires “leapfrogging” to the deployment of the newest diagnostic systems that do not have the infrastructure requirements of systems used in developed countries. Methods A laboratory for molecular diagnostics of infectious agents was established in Bo, Sierra Leone with a hybrid solar/diesel/battery system to ensure stable power supply and a satellite modem to enable efficient communication. An array of room temperature stabilization and refrigeration technologies for reliable transport and storage of reagents and biological samples were also tested to ensure sustainable laboratory supplies for diagnostic assays. Results The laboratory demonstrated its operational proficiency by conducting an investigation of a suspected avian influenza outbreak at a commercial poultry farm at Bo using broad range resequencing microarrays and real time RT-PCR. The results of the investigation excluded influenza viruses as a possible cause of the outbreak and indicated a link between the outbreak and the presence of Klebsiella pneumoniae. Conclusions This study demonstrated that by application of a carefully selected set of technologies and sufficient personnel training, it is feasible to deploy and effectively use a broad-range infectious pathogen detection technology in a severely resource-limited setting.</p

ARDM content allocations for genes conferring resistance to different classes of antimicrobial compounds.

<p>Two hundred thirty-nine different resistance determinants are represented by probes on each sub-array.</p

Genetic MDR profiles from clinical isolates, excluding ESBLs.

a<p>Abbreviations: TET – tetracyclines; CHLOR – chloramphenicol; QAC – quaternary ammonium compounds; SUL – sulfonamides; TRI – trimethoprim; FQ - fluoroquinolones.</p>b<p>Genes responsible for resistance to β-lactams, which are not included in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0069507#pone-0069507-t003" target="_blank">Table 3</a>.</p>c<p>Based on results obtained with the reference strains and PCR validation (data not shown) and may reflect false positives and/or truncated genes.</p