New England Food Vision: Healthy Food for All, Sustainable Farming and Fishing, Thriving Communities

A New England Food Vision proposes changes in food production, distribution, and consumption reaching from the most rural areas to the densest cities—across the entire food system. The New England Food Vision Writing Team has been working since 2011 to produce a bold vision that calls for our region to build the capacity to produce at least 50% of clean, fair, accessible and just food for New Englanders by 2060. The Vision includes a set of guiding assumptions and calculations that sketch a future in which diverse local and state food systems are supported by and in turn support a regional sustainable food system. The roles of the writing team include production of a New England Food Vision document, including input from regional stakeholders at the annual New England Food Summits, discussions at various meetings, presentations, and via an online survey

Work Zone Intrusion Report Interface Design

While necessary for roadways, work zones present a safety risk to crew. Half of road workers deaths between 2005 and 2010 were due to collisions with motorists intruding on the work zone. Therefore, addressing intrusions is an important step for ensuring a safe work environment for crewmembers. However, a recent research synthesis at the Minnesota Department of Transportation found that few states had an explicit method for systematically collecting work zone intrusion data. The purpose of this work zone intrusion interface design project was to design an efficient, comprehensive, and user-friendly reporting system for intrusions in work zones. A user-centric, iterative design process was employed to design an adaptable web-based and paper report to account for simple documentation of intrusions not deemed a threat to worker safety and a detailed report for more thorough documentation of serious intrusion events. Final recommendations include organizational changes and support to encourage workers to complete the form and provide valuable data to the state

Rapid and High-Throughput pan-Orthopoxvirus Detection and Identification using PCR and Mass Spectrometry

The genus Orthopoxvirus contains several species of related viruses, including the causative agent of smallpox (Variola virus). In addition to smallpox, several other members of the genus are capable of causing human infection, including monkeypox, cowpox, and other zoonotic rodent-borne poxviruses. Therefore, a single assay that can accurately identify all orthopoxviruses could provide a valuable tool for rapid broad orthopovirus identification. We have developed a pan-Orthopoxvirus assay for identification of all members of the genus based on four PCR reactions targeting Orthopoxvirus DNA and RNA helicase and polymerase genes. The amplicons are detected using electrospray ionization-mass spectrometry (PCR/ESI-MS) on the Ibis T5000 system. We demonstrate that the assay can detect and identify a diverse collection of orthopoxviruses, provide sub-species information and characterize viruses from the blood of rabbitpox infected rabbits. The assay is sensitive at the stochastic limit of PCR and detected virus in blood containing approximately six plaque-forming units per milliliter from a rabbitpox virus-infected rabbit

Alzheimer’s disease genetic risk and cognitive reserve in relationship to long-term cognitive trajectories among cognitively normal individuals

Background: Both Alzheimer’s disease (AD) genetic risk factors and indices of cognitive reserve (CR) influence risk of cognitive decline, but it remains unclear whether they interact. This study examined whether a CR index score modifies the relationship between AD genetic risk factors and long-term cognitive trajectories in a large sample of individuals with normal cognition. Methods: Analyses used data from the Preclinical AD Consortium, including harmonized data from 5 longitudinal cohort studies. Participants were cognitively normal at baseline (M baseline age = 64 years, 59% female) and underwent 10 years of follow-up, on average. AD genetic risk was measured by (i) apolipoprotein-E (APOE) genetic status (APOE-ε2 and APOE-ε4 vs. APOE-ε3; N = 1819) and (ii) AD polygenic risk scores (AD-PRS; N = 1175). A CR index was calculated by combining years of education and literacy scores. Longitudinal cognitive performance was measured by harmonized factor scores for global cognition, episodic memory, and executive function. Results: In mixed-effects models, higher CR index scores were associated with better baseline cognitive performance for all cognitive outcomes. APOE-ε4 genotype and AD-PRS that included the APOE region (AD-PRSAPOE) were associated with declines in all cognitive domains, whereas AD-PRS that excluded the APOE region (AD-PRSw/oAPOE) was associated with declines in executive function and global cognition, but not memory. There were significant 3-way CR index score × APOE-ε4 × time interactions for the global (p = 0.04, effect size = 0.16) and memory scores (p = 0.01, effect size = 0.22), indicating the negative effect of APOE-ε4 genotype on global and episodic memory score change was attenuated among individuals with higher CR index scores. In contrast, levels of CR did not attenuate APOE-ε4-related declines in executive function or declines associated with higher AD-PRS. APOE-ε2 genotype was unrelated to cognition. Conclusions: These results suggest that APOE-ε4 and non-APOE-ε4 AD polygenic risk are independently associated with global cognitive and executive function declines among individuals with normal cognition at baseline, but only APOE-ε4 is associated with declines in episodic memory. Importantly, higher levels of CR may mitigate APOE-ε4-related declines in some cognitive domains. Future research is needed to address study limitations, including generalizability due to cohort demographic characteristics

2011 Wild Blueberry Project Reports

The 2011 edition of the Wild Blueberry Project Reports was prepared for the Wild Blueberry Commission of Maine and the Wild Blueberry Advisory Committee by researchers at the University of Maine, Orono. Projects in this report include: 1. Mechanisms through which wild blueberries affect functional characteristics of the arterial wall related to endothelial dysfunction in Spontaneously Hypertensive Rats 2. Do wild blueberries affect high blood lipids, high blood glucose and obesity as related to the Metabolic Syndrome? 3. Control tactics for blueberry pest insects, 2011 4. IPM strategies, 2011 5. Biology and ecology of pest insects, 2011 6. Biology and ecology of beneficial insects, 2011 7. Pesticide residues on lowbush blueberry, 2011 8. Maine wild blueberry –mummy berry research and extension 9. Valdensinia leaf spot – Education, control and research – 2011 10. Effect of soil pH on nutrient uptake 11. Evaluation of several growth regulators for stimulating lowbush blueberry rhizome production 12. Evaluation of the effects of Gypsum (CaSO4) on wild blueberry fruit Ca concentration 13. Fall application of tribenuron methyl for bunchberry control in wild blueberries, 2009-2011 – effects of fall versus spring pruning on cover and yield in the crop year (final report) 14. Wild Blueberry Extension Education Program in 2011 INPUT SYSTEMS STUDY: 15. Systems approach to improving the sustainability of wild blueberry production, Year 2 of a four-year study – experimental design 16. The effects of different agronomic practices on the composition and quality of wild blueberries 17. Systems approach to improving the sustainability of wild blueberry production, Year 2 of a four-year study, blueberry quality from the first harvest year 18. Food safety- Prevalence study of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. on lowbush blueberries (Vaccinium angustifolium) 19. Abundance of insect pest species and natural enemies in lowbush blueberry fields maintained under different management practices 20. Systems approach to improving the sustainability of wild blueberry production, Year 2 of a four-year study, disease management results 21. Systems approach to improving the sustainability of wild blueberry production, Year 2 of a four-year study, weed management results 22. Systems approach to improving sustainability of wild blueberry production, Year 1 of a four-year study, preliminary soil health and chemistry results 23. Management effects on the phosphorus chemistry of wild blueberry barrens soil 24. Systems approach to improving the sustainability of wild blueberry production, preliminary economic comparison 2010-2011 25. Transition to organic production Year 2: Evaluation of gypsum and bone char 26. The effect of field management, forest edge composition, and field location on insect-mediated ecosystem services (scavenging/predation) (ancillary study) 27. Pre-emergent combinations of herbicides for weed control in wild blueberry fields (ancillary study) 28. Systems approach to improving the sustainability of wild blueberry production – Ancillary land-leveling study, year 1 (ancillary study) 29. Effects of compost and mulch on soil health and soil nutrient dynamics in wild blueberry (ancillary study

The AIM2 inflammasome exacerbates atherosclerosis in clonal haematopoiesis

Clonal haematopoiesis, which is highly prevalent in older individuals, arises from somatic mutations that endow a proliferative advantage to haematopoietic cells. Clonal haematopoiesis increases the risk of myocardial infarction and stroke independently of traditional risk factors(1). Among the common genetic variants that give rise to clonal haematopoiesis, the JAK2(V617F) (JAK2(VF)) mutation, which increases JAK-STAT signalling, occurs at a younger age and imparts the strongest risk of premature coronary heart disease(1,2). Here we show increased proliferation of macrophages and prominent formation of necrotic cores in atherosclerotic lesions in mice that express Jak2(VF) selectively in macrophages, and in chimeric mice that model clonal haematopoiesis. Deletion of the essential inflammasome components caspase 1 and 11, or of the pyroptosis executioner gasdermin D, reversed these adverse changes. Jak2(VF) lesions showed increased expression of AIM2, oxidative DNA damage and DNA replication stress, and Aim2 deficiency reduced atherosclerosis. Single-cell RNA sequencing analysis of Jak2(VF) lesions revealed a landscape that was enriched for inflammatory myeloid cells, which were suppressed by deletion of Gsdmd. Inhibition of the inflammasome product interleukin-1 beta reduced macrophage proliferation and necrotic formation while increasing the thickness of fibrous caps, indicating that it stabilized plaques. Our findings suggest that increased proliferation and glycolytic metabolism in Jak2(VF) macrophages lead to DNA replication stress and activation of the AIM2 inflammasome, thereby aggravating atherosclerosis. Precise application of therapies that target interleukin-1 beta or specific inflammasomes according to clonal haematopoiesis status could substantially reduce cardiovascular risk