Intracellular Localization and Some Properties of Cytochrome P450 of Rhizopus nigricans

An attempt was made to localize in Rhizopus nigricans the enzyme cytochrome P450, which is required for the hydroxylation of progesterone. Cytochrome P450 was never found in the mitochondrial fraction but was detected in the fraction sedimenting at 105000 g. In R. nigricans this fraction does not seem to be identical with the rat liver microsomal fraction in respect to the enzyme activities characteristic for microsomes. In the . presence of progesterone the subcellular fraction containing cytochrome P450 exhibits modified type II spectral change. Upon solubilization of this fraction with detergent Triton X-100 a significant amount of the enzyme is converted to its inactive form P420

"An effective two dimensionality" cases bring a new hope to the Kaluza-Klein[like] theories

One step towards realistic Kaluza-Klein[like] theories and a loop hole through the Witten's "no-go theorem" is presented for cases which we call an effective two dimensionality cases: In $d=2$ the equations of motion following from the action with the linear curvature leave spin connections and zweibeins undetermined. We present the case of a spinor in $d=(1+5)$ compactified on a formally infinite disc with the zweibein which makes a disc curved on an almost $S^2$ and with the spin connection field which allows on such a sphere only one massless normalizable spinor state of a particular charge, which couples the spinor chirally to the corresponding Kaluza-Klein gauge field. We assume no external gauge fields. The masslessness of a spinor is achieved by the choice of a spin connection field (which breaks parity), the zweibein and the normalizability condition for spinor states, which guarantee a discrete spectrum forming the complete basis. We discuss the meaning of the hole, which manifests the noncompactness of the space.Comment: 26 pages, 1 figure, an addition which helps to clarify the assumptions and their consequences (the discreteness of spectrum, the massless solution of one handedness,..