Current Strategies for Identification of Glioma Stem Cells: Adequate or Unsatisfactory?

Cancer stem cells (CSCs) were isolated in multiple tumor types, including human glioblastomas, and although the presence of surface markers selectively expressed on CSCs can be used to isolate them, no marker/pattern of markers are sufficiently robust to definitively identify stem cells in tumors. Several markers were evaluated for their prognostic value with promising early results, however none of them was proven to be clinically useful in large-scale studies, leading to outstanding efforts to identify new markers. Given the heterogeneity of human glioblastomas further investigations are necessary to identify both cancer stem cell-specific markers and the molecular mechanisms sustaining the tumorigenic potential of these cells to develop tailored treatments. Markers for glioblastoma stem cells such as CD133, CD15, integrin-α6, L1CAM might be informative to identify these cells but cannot be conclusively linked to a stem cell phenotype. Overlap of expression, functional state and morphology of different subpopulations lead to carefully consider the techniques employed so far to isolate these cells. Due to a dearth of methods and markers reliably identifying the candidate cancer stem cells, the isolation/enrichment of cancer stem cells to be therapeutically targeted remains a major challenge

Realidad aumentada, realidad virtual y aprendizaje en el contexto educativo superior a nivel internacional

Objetivo: analizar la realidad aumentada, realidad virtual y el uso de las TIC en el aprendizaje de la enseñanza superior a través de la revisión de la literatura. Método: en total se revisó 23 bases de datos. Dicha búsqueda permitió que se conforme un corpus de 15 artículos académicos publicados en castellano entre 2015 y 2020 sobre realidad virtual en el contexto educativo. Resultados: El uso de la realidad aumentada en el sector educativo a nivel internacional es en promedio de un 37 %. Actualmente este porcentaje va en aumento debido a la disminución de precios en el mercado respecto de esta tecnología, lo cual la hace más accesible. El número de investigaciones científicas sobre realidad virtual ha ido aumentando y se estima que la publicación de artículos creció de 0,27 % en 1998 a 14,48 % en 2018. En el campo de la educación el 86 % de los estudiantes se encuentran más motivados en un salón de clase que cuenta con una computadora e Internet. Conclusión: el avance de la tecnología ha logrado cambios importantes en la vida del ser humano, tanto en la realidad virtual como aumentada. Con ella se puede experimentar diferentes emociones y solucionar posibles problemas desde lo simple hasta lo más complejo. Los pasos acrecentados de la tecnología están maximizando la motivación en distintos campos de la educación como carreras enfocadas en el desarrollo de química, medicina, neurociencia y videojuegos a través de la creación de software en realidad aumentada y realidad virtual

On the Role of Support in Metallic Heterogeneous Catalysis: A Study of Unsupported Nickel\u2013Cobalt Alloy Nanoparticles in Ethanol Steam Reforming

(Co, Ni) bimetallic nanoparticles have been prepared by reducing Ni and Co chloride solutions with sodium borohydride. The obtained materials have been characterized as cast and/or after annealing by means of XRD, magnetic measurements, IR spectroscopy, FE-SEM and TEM microscopies. The resulting nanomaterials, originally amorphous, crystallize into the cubic structure cF4-Cu as homogeneous (Co, Ni) solid solution alloy and with the additional presence of Boron containing phases due to the residual preparation impurities. The bimetallic nanoparticles are active in ethanol conversion in the presence of steam. For low Boron catalysts, the addition of Nickel to Cobalt nanoparticles improves the catalytic activity in ethanol steam reforming allowing yields as high as 87% at 773 K, at high space velocities (GHSV 324,000 h 121 ). The performances of the catalytic unsupported nanoparticles with a Ni/Co atomic ratio equal to 0.26 appear to be better than those of conventional supported catalysts. The state of Boron impurities affect catalytic activity of bimetallic (Co, Ni) NPs. Carbonaceous materials, such as carbon nanotubes and graphitic carbon, form on the catalyst surface upon reaction. Graphical Abstract: [Figure not available: see fulltext.]

Off-Adherence Keeping (OAK) observational study: intentional off-adherence immunomodulatory multiple sclerosis treatment

Aims: To evaluate how improved treatment adherence with a lower-frequency regimen/treatment of intramuscular (IM) IFN beta-1a impacts therapeutic effectiveness in relapsing-remitting multiple sclerosis (MS) patients switching from a higher-frequency injectable regimen/treatment. Patients & methods: Italian patients with relapsing-remitting MS and prior poor adherence to high-frequency injectable treatments (n = 181) were followed for 24 months after starting IM IFN beta-1a. Results: During the study, 97.4% of patients were treatment adherent; 22.1% of patients reported a relapse. The estimated probability of remaining relapse-free after 2 years was 78%. A high dropout rate (52.5%) led to small sample size and reduced statistical power. Conclusion: Intramuscular IFN beta-1a treatment was associated with high adherence and a low relapse rate. Unfortunately, low patient retention limited the generalizability of these findings.Plain language summary: Prior research suggests that taking the drug IFN beta-1a through less frequent muscle injections enables more patients to adhere to their prescription than taking other medications. This study included 181 Italian patients with relapsing-remitting multiple sclerosis (MS) who historically did not take medication as often as prescribed. Relapses of MS were counted among patients treated with muscle injections of IFN beta-1a for 2 years; 97.4% of patients followed their prescription and 22.1% experienced a relapse. From these data, 78% of patients were estimated not to experience a relapse during 2 years of IFN beta-1a muscle injections. However, an unusually high number of patients (52.5%) left the study within 2 years, which makes it difficult to draw firm conclusions

Microbiota-driven gut vascular barrier disruption is a prerequisite for non-alcoholic steatohepatitis development.

BACKGROUND & AIMS Fatty liver disease, including non-alcoholic fatty liver (NAFLD) and steatohepatitis (NASH), has been associated with increased intestinal barrier permeability and translocation of bacteria or bacterial products into the blood circulation. In this study, we aimed to unravel the role of both intestinal barrier integrity and microbiota in NAFLD/NASH development. METHODS C57BL/6J mice were fed with high-fat diet (HFD) or methionine-choline-deficient diet for 1 week or longer to recapitulate aspects of NASH (steatosis, inflammation, insulin resistance). Genetic and pharmacological strategies were then used to modulate intestinal barrier integrity. RESULTS We show that disruption of the intestinal epithelial barrier and gut vascular barrier (GVB) are early events in NASH pathogenesis. Mice fed HFD for only 1 week undergo a diet-induced dysbiosis that drives GVB damage and bacterial translocation into the liver. Fecal microbiota transplantation from HFD-fed mice into specific pathogen-free recipients induces GVB damage and epididymal adipose tissue enlargement. GVB disruption depends on interference with the WNT/β-catenin signaling pathway, as shown by genetic intervention driving β-catenin activation only in endothelial cells, preventing GVB disruption and NASH development. The bile acid analogue and farnesoid X receptor agonist obeticholic acid (OCA) drives β-catenin activation in endothelial cells. Accordingly, pharmacologic intervention with OCA protects against GVB disruption, both as a preventive and therapeutic agent. Importantly, we found upregulation of the GVB leakage marker in the colon of patients with NASH. CONCLUSIONS We have identified a new player in NASH development, the GVB, whose damage leads to bacteria or bacterial product translocation into the blood circulation. Treatment aimed at restoring β-catenin activation in endothelial cells, such as administration of OCA, protects against GVB damage and NASH development. LAY SUMMARY The incidence of fatty liver disease is reaching epidemic levels in the USA, with more than 30% of adults having NAFLD (non-alcoholic fatty liver disease), which can progress to more severe non-alcoholic steatohepatitis (NASH). Herein, we show that disruption of the intestinal epithelial barrier and gut vascular barrier are early events in the development of NASH. We show that the drug obeticholic acid protects against barrier disruption and thereby prevents the development of NASH, providing further evidence for its use in the prevention or treatment of NASH

Planeamiento estratégico de la alcachofa

El plan estratégico de la Alcachofa tiene como objetivo principal establecer las estrategias que le permitan impulsar su desarrollo y llevar al país a ser reconocido como el segundo exportador a nivel mundial, contribuyendo así al desarrollo económico y respetando las políticas que rigen la protección, cuidado del medio ambiente y bienestar de la sociedad. Asimismo, un punto a favor para la producción de la Alcachofa son los precios altos que se pagan en el mercado internacional ya que este producto es comercializado en forma de conserva por la gran demanda principalmente de Estados Unidos y algunos países de Europa como España y Francia, manteniendo un ritmo creciente debido al reconocimiento de sus altas propiedades nutritivas. El presente documento muestra como resultado de la evaluación externa e interna de la Alcachofa, 30 factores, que serán el input para definir un conjunto de estrategias, políticas, objetivos a corto plazo, objetivos de largo plazo y métricas de evaluación estratégica que lleven a la Alcachofa a lograr la visión trazada al 2023The main purpose of the Artichoke strategic plan is to establish the strategies that allow it to encourage its development and lead Peru to be acknowledged as the second exporting country worldwide, thus contributing to the economic development and following the policies governing the protection, environmental care and wellness of the society. Additionally, the high prices paid in the international market for the Artichoke are an additional plus seeing that this product is traded as canned foods due to the high demand mainly in the United States and some European countries such as Spain and France, while maintaining an increasing pace due to its nutritional properties acknowledgement. As a result of the external and internal evaluation of the Artichoke, this document shows 30 factors, that will be the input to define a set of strategies, policies, short-tem goals, long-term goals and strategic assessment metrics that lead to the vision of the Artichoke for 2023Tesi

Shapley Supercluster Survey: Construction of the photometric catalogues and i-band data release

The Shapley Supercluster Survey is a multi-wavelength survey covering an area of ~23 deg2 (~260 Mpc2 at z = 0.048) around the supercluster core, including nine Abell and two poor clusters, having redshifts in the range 0.045-0.050. The survey aims to investigate the role of the cluster-scale mass assembly on the evolution of galaxies, mapping the effects of the environment from the cores of the clusters to their outskirts and along the filaments. The optical (ugri) imaging acquired with OmegaCAM on the VLT Survey Telescope is essential to achieve the project goals providing accurate multi-band photometry for the galaxy population down to m * + 6. We describe the methodology adopted to construct the optical catalogues and to separate extended and point-like sources. The catalogues reach average 5s limitingmagnitudes within a 3 arcsec diameter aperture of ugri=[24.4,24.6,24.1,23.3] and are 93 per cent complete down to ugri = [23.8,23.8,23.5,22.0] mag, corresponding to ~mr * + 8.5. The data are highly uniform in terms of observing conditions and all acquired with seeing less than 1.1 arcsec full width at half-maximum. The median seeing in r band is 0.6 arcsec, corresponding to 0.56 kpc h70 -1 at z = 0.048. While the observations in the u, g and r bands are still ongoing, the i-band observations have been completed, and we present the i-band catalogue over the whole survey area. The latter is released and it will be regularly updated, through the use of the Virtual Observatory tools. This includes 734 319 sources down to i = 22.0 mag and it is the first optical homogeneous catalogue at such a depth, covering the central region of the Shapley supercluster

Risk of Getting COVID-19 in People With Multiple Sclerosis

Background and Objectives Several studies have assessed risk factors associated with the severity of COVID-19 outcomes in people with multiple sclerosis (PwMS). The potential role of disease-modifying therapies (DMTs) and demographic and clinical factors on the risk of acquiring SARS-CoV-2 infection has not been evaluated so far. The objective of this study was to assess risk factors of contracting SARS-CoV-2 infection in PwMS by using data collected in the Italian MS Register (IMSR). Methods Acase-control (1:2) studywas set up. Cases included PwMSwith a confirmed diagnosis ofCOVID-19, and controls included PwMS without a confirmed diagnosis of COVID-19. Both groups were propensity score–matched by the date of COVID-19 diagnosis, the date of last visit, and the region of residence. No healthy controls were included in this study. COVID-19 risk was estimated by multivariable logistic regression models including demographic and clinical covariates. The impact of DMTs was assessed in 3 independent logistic regression models including one of the following covariates: last administeredDMT, previousDMTsequences, or the place where the last treatment was administered. Results A total of 779 PwMS with confirmed COVID-19 (cases) were matched to 1,558 PwMS without COVID-19 (controls). In all 3 models, comorbidities, female sex, and a younger age were significantly associated (p < 0.02)with a higher risk of contractingCOVID-19. Patients receiving natalizumab as last DMT(OR[95%CI]: 2.38 [1.66–3.42], p < 0.0001) and those who underwent an escalation treatment strategy (1.57 [1.16–2.13], p = 0.003) were at significantly higher COVID-19 risk. Moreover, PwMS receiving their last DMT requiring hospital access (1.65 [1.34–2.04], p < 0.0001) showed a significant higher risk than those taking self-administered DMTs at home. Discussion This case-control study embedded in the IMSR showed that PwMS at higher COVID-19 risk are younger, more frequently female individuals, and with comorbidities. Long-lasting escalation approach and last therapies that expose patients to the hospital environment seem to significantly increase the risk of SARS-CoV2 infection in PwMS. Classification of Evidence This study provides Class III evidence that among patients with MS, younger age, being female individuals, having more comorbidities, receiving natalizumab, undergoing an escalating treatment strategy, or receiving treatment at a hospital were associated with being infected with COVID-19. Among patients with MS who were infected with COVID-19, a severe course was associated with increasing age and having a progressive form of MS, whereas not being on treatment or receiving an interferon beta agent was protective