Structure and stellar content of dwarf galaxies. III: B and R photometry of dwarf galaxies in the M101 group and the nearby field

We have carried out CCD photometry in the Cousins B and R bands of 21 dwarf galaxies in and around the M101 group. Eleven are members and suspected members of the group and ten are field galaxies in the projected vicinity of the group. We have derived total magnitudes, effective radii, effective surface brightnesses, as well as galaxy diameters at various isophotal levels in both colours. Best-fitting exponential parameters and colour gradients are also given for these galaxies. Some of the galaxies show a pronounced luminosity excess above the best-fitting exponential at large radii, or surface brightnesses fainter than approx 26 mag/sq_arcsec in R. This feature, while non-significant for a single case and technically difficult to interpret, might be an indication of the existence of an extended old stellar halo in dwarf irregulars. The photometric parameters of the galaxies presented here will be combined with previously published data for nearby dwarf galaxies and statistically analysed in a forthcoming paper.Comment: 15 pages, 4 tables and 13 figures. For a full resolution version see http://www.astro.unibas.ch/galaxies/papers.html To appear in A&A

Structure and stellar content of dwarf galaxies IV. B and R photometry of dwarf galaxies in the CVnI cloud

We have carried out CCD photometry in the Cousins B and R bands of 15 galaxies in the Canes Venatici I cloud. Total magnitudes, effective radii, effective surface brightnesses, as well as galaxy radii at various isophotal levels in both colors were determined. Best-fitting exponential parameters and color gradients are also given for these galaxies. The photometric parameters presented here will analyzed in a forthcoming paper, together with previously published data for nearby dwarf galaxies.Comment: 10 pages, submitted to A&AS. For a full resolution version see ftp://merkur.astro.unibas.ch/pub/bremnes/canesv.ps.g

Changes in the secretory profile of NSCLC-associated fibroblasts after ablative radiotherapy: potential impact on angiogenesis and tumor growth

In the context of radiotherapy, collateral effects of ablative ionizing radiation (AIR) on stromal components of tumors remains understudied. In this work, cancer-associated fibroblasts (CAFs) isolated from freshly resected human lung tumors were exposed to AIR (1x18Gy) and analyzed for their release of paracrine factors. Inflammatory mediators and regulators of angiogenesis and tumor growth were analyzed by multiplex protein assays in conditioned medium (CM) from irradiated and non-irradiated CAFs. Additionally, the profile of secreted proteins was examined by proteomics. In functional assays, effects of CAF-CM on proliferative and migratory capacity of lung tumor cells (H-520/H-522) and endothelial cells (HUVECs), and on the tube-forming capacity of endothelial cells was assessed. Our data show that exposure of CAFs to ablative doses of ionizing radiation results in a) down-regulated release of angiogenic factors SDF-1, angiopoietin and thrombospondin-2; b) up-regulated release of growth factor bFGF from most donors, and c) unaffected expression-levels of HGF and inflammatory mediators IL-6, IL-8, IL-1ƒÒ and TNF-£. Conditioned medium from irradiated and control CAFs did not affect differently the proliferative or migratory capacity of tumor cells (H-520/H-522), whereas migratory capacity of endothelial HUVEC cells was partially reduced in the presence of irradiated CAF conditioned medium. Overall we conclude that AIR mediates a transformation on the secretory profile of CAFs that could influence the behavior of other cells in the tumor tissue and hence guide to some extent therapeutic outcomes. The downstream consequences of the changes observed in this study merits further investigations

Using conditional kernel density estimation for wind power density forecasting

Of the various renewable energy resources, wind power is widely recognized as one of the most promising. The management of wind farms and electricity systems can benefit greatly from the availability of estimates of the probability distribution of wind power generation. However, most research has focused on point forecasting of wind power. In this paper, we develop an approach to producing density forecasts for the wind power generated at individual wind farms. Our interest is in intraday data and prediction from 1 to 72 hours ahead. We model wind power in terms of wind speed and wind direction. In this framework, there are two key uncertainties. First, there is the inherent uncertainty in wind speed and direction, and we model this using a bivariate VARMA-GARCH (vector autoregressive moving average-generalized autoregressive conditional heteroscedastic) model, with a Student t distribution, in the Cartesian space of wind speed and direction. Second, there is the stochastic nature of the relationship of wind power to wind speed (described by the power curve), and to wind direction. We model this using conditional kernel density (CKD) estimation, which enables a nonparametric modeling of the conditional density of wind power. Using Monte Carlo simulation of the VARMA-GARCH model and CKD estimation, density forecasts of wind speed and direction are converted to wind power density forecasts. Our work is novel in several respects: previous wind power studies have not modeled a stochastic power curve; to accommodate time evolution in the power curve, we incorporate a time decay factor within the CKD method; and the CKD method is conditional on a density, rather than a single value. The new approach is evaluated using datasets from four Greek wind farms

A Multi-wavelength analysis of M81: insight on the nature of Arp's loop

Context: The optical ring like structure detected by Arp (1965) around M81 (commonly referenced as "Arp's loop") represents one of the most spectacular feature observed in nearby galaxies. Arp's loop is commonly interpreted as a tail resulting from the tidal interaction between M81 and M82. However, since its discovery the nature of this feature has remained controversial. Aims: Our primary purpose was to identify the sources of optical and infrared emission observed in Arp's loop. Methods: The morphology of the Arp's loop has been investigated with deep wide-field optical images. We also measured its colors using IRAS and Spitzer-MIPS infrared images and compared them with those of the disk of M81 and Galactic dust cirrus that fills the area where M81 is located. Results: Optical images reveal that this peculiar object has a filamentary structure characterized by many dust features overlapping M81's field. The ratios of far-infrared fluxes and the estimated dust-to-gas ratios indicate the infrared emission of Arp's loop is dominated by the contribution of cold dust that is most likely from Galactic cirrus. Conclusions: The above results suggest that the light observed at optical wavelengths is a combination of emission from i) a few recent star forming regions located close to M81, where both bright UV complexes and peaks in the HI distribution are found, ii) the extended disk of M81 and iii) scattered light from the same Galactic cirrus that is responsible for the bulk of the far infrared emission.Comment: 7 pages, 7 figures, accepted for publication by A&

Cancer-associated fibroblasts from human NSCLC survive ablative doses of radiation but their invasive capacity is reduced

<p>Background: Cancer-Associated Fibroblasts (CAFs) are significant components of solid malignancies and play central roles in cancer sustainability, invasion and metastasis. In this study we have investigated the invasive capacity and matrix remodelling properties of human lung CAFs after exposure to ablative doses of ionizing radiation (AIR), equivalent to single fractions delivered by stereotactic ablative radiotherapy (SART) for medically inoperable stage-I/II non-small-cell lung cancers.</p> <p>Methods: CAFs were isolated from lung tumour specimens from 16 donors. Initially, intrinsic radiosensitivity was evaluated by checking viability and extent of DNA-damage response (DDR) at different radiation doses. The migrative and invasive capacities of CAFs were thereafter determined after a sub-lethal single radiation dose of 18 Gy. To ascertain the mechanisms behind the altered invasive capacity of cells, expression of matrix metalloproteinases (MMPs) and their endogenous inhibitors (TIMPs) were measured in the conditioned media several days post-irradiation, along with expression of cell surface integrins and dynamics of focal contacts by vinculin-staining.</p> <p>Results: Exposing CAFs to 1 × 18 Gy resulted in a potent induction of multiple nuclear DDR foci (> 9/cell) with little resolution after 120 h, induced premature cellular senescence and inhibition of the proliferative, migrative and invasive capacity. AIR promoted MMP-3 and inhibited MMP-1 appearance to some extent, but did not affect expression of other major MMPs. Furthermore, surface expression of integrins α2, β1 and α5 was consistently enhanced, and a dramatic augmentation and redistribution of focal contacts was observed.</p> <p>Conclusions: Our data indicate that ablative doses of radiation exert advantageous inhibitory effects on the proliferative, migratory and invasive capacity of lung CAFs. The reduced motility of irradiated CAFs might be a consequence of stabilized focal contacts via integrins.</p&gt

Prognostic impact of peritumoral lymphocyte infiltration in soft tissue sarcomas

<p>Abstract</p> <p>Background</p> <p>The purpose of this study was to clarify the prognostic significance of peritumoral lymphocyte infiltration in the capsule of soft tissue sarcomas (STS). Multiple observations in preclinical and clinical studies have shown that the immune system has a role in controlling tumor growth and progression. Prognostic markers in potentially curable STS should guide therapy after surgical resection. The immune status at the time of resection may be important, but the prognostic significance of peritumoral lymphocytes is unknown.</p> <p>Methods</p> <p>Tissue microarrays from 80 patients with STS were constructed from duplicate cores of tissue from the tumor and the peritumoral capsule. Immunohistochemistry was used to evaluate the CD3+, CD4+, CD8+ and CD20+ lymphocytes in the tumor and the peritumoral capsule.</p> <p>Results</p> <p>In univariate analyses, increasing numbers of CD20+ (<it>P </it>= 0.032) peritumoral lymphocytes were associated with a reduced disease free survival (DSS). In multivariate analyses, a high number of CD20+ peritumoral lymphocytes (<it>P </it>= 0.030) in the capsule was an independent negative prognostic factor for DSS. There were no such associations of lymphocyte infiltration in the tumor.</p> <p>Conclusions</p> <p>A high density of CD20+ peritumoral lymphocytes is an independent negative prognostic indicator for patients with STS. Further research is needed to determine whether CD20 cells in the peritumoral capsule of STS may promote tumor invasion in the surrounding tissue and increase the metastatic potential.</p

The Gas Content in Galactic Disks: Correlation with Kinematics

We consider the relationship between the total HI mass in late-type galaxies and the kinematic properties of their disks. The mass $M_HI$ for galaxies with a wide variety of properties, from dwarf dIrr galaxies with active star formation to giant low-brightness galaxies, is shown to correlate with the product $V_c R_0$ ($V_c$ is the rotational velocity, and $R_0$ is the radial photometric disks scale length), which characterizes the specific angular momentum of the disk. This relationship, along with the anticorrelation between the relative mass of HI in a galaxy and $V_c$, can be explained in terms of the previously made assumption that the gas density in the disks of most galaxies is maintained at a level close to the threshold (marginal) stability of a gaseous layer to local gravitational perturbations. In this case, the regulation mechanism of the star formation rate associated with the growth of local gravitational instability in the gaseous layer must play a crucial role in the evolution of the gas content in the galactic disk.Comment: revised version to appear in Astronomy Letters, 8 pages, 5 EPS figure

Genotypes and haplotypes in the insulin-like growth factors, their receptors and binding proteins in relation to plasma metabolic levels and mammographic density

<p>Abstract</p> <p>Background</p> <p>Increased mammographic density is one of the strongest independent risk factors for breast cancer. It is believed that one third of breast cancers are derived from breasts with more than 50% density. Mammographic density is affected by age, BMI, parity, and genetic predisposition. It is also greatly influenced by hormonal and growth factor changes in a woman's life cycle, spanning from puberty through adult to menopause. Genetic variations in genes coding for hormones and growth factors involved in development of the breast are therefore of great interest. The associations between genetic polymorphisms in genes from the IGF pathway on mammographic density and circulating levels of IGF1, its binding protein IGFBP3, and their ratio in postmenopausal women are reported here.</p> <p>Methods</p> <p>Samples from 964 postmenopausal Norwegian women aged 55-71 years were collected as a part of the Tromsø Mammography and Breast Cancer Study. All samples were genotyped for 25 SNPs in IGF1, IGF2, IGF1R, IGF2R, IGFALS and IGFBP3 using Taqman (ABI). The main statistical analyses were conducted with the PROC HAPLOTYPE procedure within SAS/GENETICS™ (SAS 9.1.3).</p> <p>Results</p> <p>The haplotype analysis revealed six haploblocks within the studied genes. Of those, four had significant associations with circulating levels of IGF1 or IGFBP3 and/or mammographic density. One haplotype variant in the IGF1 gene was found to be associated with mammographic density. Within the IGF2 gene one haplotype variant was associated with levels of both IGF1 and IGFBP3. Two haplotype variants in the IGF2R were associated with the level of IGF1. Both variants of the IGFBP3 haplotype were associated with the IGFBP3 level and indicate regulation in cis.</p> <p>Conclusion</p> <p>Polymorphisms within the IGF1 gene and related genes were associated with plasma levels of IGF1, IGFBP3 and mammographic density in this study of postmenopausal women.</p