Modelling respiration rate of fresh fruits and vegetables for modified atmosphere packages: a review

Respiration rate and gas exchange through the package material are the processes involved in creating a modified atmosphere inside a package that will extend shelf life of fresh fruits and vegetables. Thus, modelling respiration rate of the selected produce is crucial to the design of a successful modified atmosphere packaging (MAP) system. In this paper, general aspects of the respiration process are presented. The major methods for measuring respiration rates, along with their advantages and limitations are discussed. Factors affecting the respiration rate and respiratory quotient are outlined, stressing the importance of temperature, O2 and CO2 concentrations, and storage time. Respiration rate models in the literature are also reviewed. 2002 Elsevier Science Ltd. All rights reserved

Influence of low oxygen and high carbon dioxide on shredded Galega kale quality for development of modified atmosphere packages

Respiration rate, sensory attributes, colour alterations, and water, chlorophyll and ascorbic acid contents were monitored during storage of shredded Galega kale (Brassica oleracea var. acephala DC.) at 20 ◦C to define an adequate range of O2 and CO2 partial pressures for product preservation. Different low O2 and high CO2 atmospheres were tested. First, tolerance to low O2 partial pressures (1, 2, 3 or 21 kPa O2 with balance N2) was tested. Quality retention was improved as O2 partial pressure was reduced and there was no induction of anaerobic respiration. Then, tolerance to high CO2 partial pressures (0, 10, 15 or 20 kPa CO2 plus 21 kPa O2 and balance N2) was tested. The high CO2 partial pressures extended the shelf life of the shredded kale and no symptoms of CO2 injury were detected. Finally, combinations of low O2 and high CO2 (1 or 2 kPa O2 plus 15 or 20 kPa CO2, with balance N2, and an air control) were analysed. No differences were observed among the different gas combinations. An atmosphere of 1–2 kPa O2 plus 15–20 kPa CO2 and balance N2 extends the shelf life of shredded Galega kale to 4–5 days at 20◦C, compared with 2–3 days in air storage. Predictive models of chlorophyll a and b degradation as a function of time and gas composition were developed

Modelling O2 and CO2 exchange for development of perforation mediated modifed atmosphere packaging

Perforation-mediated modi®ed atmosphere packaging relies on the use of macro perforations or tubes to control the O2 and CO2 exchange and create the desired atmosphere inside an otherwise gas-tight package. In this work, the O2 and CO2 exchange through a single tube was studied. Di erent temperatures (5±20°C) and tube dimensions (length from 9 to 17 mm and diameter from 6 to 30 mm) were tested. O2 and CO2 mass transfer coe cients were determined according to a lumped mass transfer capacitance model that yielded a good description of the gas transfer. Temperature in the range tested did not show a signi®cant e ect on the mass transfer coe cients. A multiplicative non-linear equation was found to yield a good prediction of the dependence of the O2 mass transfer coe cient on tube diameter and length. The ratio between the CO2 and O2 mass transfer coe cients, an important parameter in the design of MAP, was 0.81 and none of the factors tested in¯uenced its value

Physicochemical changes during strawberry development in the field compared with those that occur in harvested fruit during storage

‘Chandler’, ‘Oso Grande’ and ‘Sweet Charlie’ strawberry cultivars were harvested at four stages of color development ranging from color break to full red and stored in the dark for 8 days at 1 ◦C. Changes in the physical and chemical characteristics of fruit during storage were compared with those occurring during development in the field. Strawberry fruit harvested at the three-quarters colored and full red stages continued their development and ripening during storage. However, fruit harvested at the color break and half-colored stages did not develop like those ripened in the field. Strawberries harvested at the three-quarters colored stage developed the same pH, acidity, soluble solids, ascorbic acid and total phenolics content during storage as the at-harvest levels present in strawberries harvested at the full red stage. In addition, three-quarters colored strawberries were as firm and red after storage as the full red stage fruit were at the time of harvest. Thus, strawberries harvested at the three-quarters colored stage can be stored for a longer period than strawberries harvested full red while retaining better color and firmness

Modelling respiration rate of shredded Galega kale for development of modified atmosphere packaging

The design of modified atmosphere packaging (MAP) for fresh-cut produce requires an adequate model for prediction of respiration rate as a function of both temperature and gas composition. In this work, the O2 consumption and CO2 production rates of shredded Galega kale were studied. The storage temperatures used were 1, 5, 10, 15 and 20 C. The atmospheres tested were all combinations of 1, 5 and 10% v/v O2 plus 0, 10 and 20% v/v CO2 with the balance being N2, as well as ambient air. Temperature was the variable with the greatest influence on respiration rate and the effect of gas composition increased with temperature. The dependence of respiration rate on gas composition was well described by a Michaelis–Menten type equation with uncompetitive CO2 inhibition. The respiratory quotient (RQ) was found to be constant for the range of temperatures and gas compositions tested and was equal to 0:93 0:01. The constants of the Michaelis–Menten equation increased exponentially with temperature. The change over time of respiration rate of leaves exposed to air at 20 C was also analysed. It was observed that respiration rate decreased with time and that the ratio between the respiration rate of shredded and intact leaves was approximately constant in the period tested and equal to 2.8. 2002 Elsevier Science Ltd. All rights reserved

Review of Challenges and Advances in Modification of Food Package Headspace Gases

Modified Atmosphere Packaging (MAP) has been widely used as an effective way to preserve foods. Fresh produce, meat and meat products, seafood, and dairy products can benefit from modified gaseous atmospheres, which are usually achieved by reducing oxygen and increasing carbon dioxide concentrations, within limits, defined by product tolerances. MAP of fresh produce is particularly challenging because products are living and respiring. Respiration rates depend on several factors including temperature, oxygen, and carbon dioxide concentrations. Balancing package permeation with respiration is challenging, often due to limited selection of practical packaging materials. Failing to remain within tolerance limits of products leads to rapid quality loss. Gas barrier properties of packages determined rate of gas exchange with the external environment and is a critical factor for achieving tolerable levels. Availability of packaging materials that meet requirement of specific produce is essential. Relative permeability of common films to carbon dioxide is about 3 to 6 times of that to oxygen, often leading to package collapse for package atmospheres that benefit from carbon dioxide. Films often fail to provide desired oxygen transmission rates, high carbon dioxide to oxygen selectivity and desired mechanical properties simultaneously. Despite advances, minimal availability and high cost of selective barrier films limit applications of MAP for fresh produce packaging. Therefore, active packaging components and films are being developed and designed to overcome these limitations. Inserts or films that contain active mixtures as gas emitters and/or scavengers are now commercially available. “Clean label” trends are motivating alternative approaches using active packaging components

C-Jun N-terminal kinase (JNK) isoforms play differing roles in otitis media

BACKGROUND: Innate immunity and tissue proliferation play important roles in otitis media (OM), the most common disease of childhood. CJUN terminal kinase (JNK) is potentially involved in both processes. RESULTS: Genes involved in both innate immune and growth factor activation of JNK are upregulated during OM, while expression of both positive and negative JNK regulatory genes is altered. When compared to wildtypes (WTs), C57BL/6 mice deficient in JNK1 exhibit enhanced mucosal thickening, with delayed recovery, enhanced neutrophil recruitment early in OM, and delayed bacterial clearance. In contrast, JNK2(−/−) mice exhibit delayed mucosal hyperplasia that eventually exceeds that of WTs and is slow to recover, delayed recruitment of neutrophils, and failure of bacterial clearance. CONCLUSIONS: The results suggest that JNK1 and JNK2 play primarily opposing roles in mucosal hyperplasia and neutrophil recruitment early in OM. However, both isoforms are required for the normal resolution of middle ear infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12865-014-0046-z) contains supplementary material, which is available to authorized users

Probing intermediates in the activation cycle of [NiFe] hydrogenase by infrared spectroscopy: the Ni-SIr state and its light sensitivity

The [NiFe] hydrogenase from the sulphate-reducing bacterium Desulfovibrio vulgaris Miyazaki F is reversibly inhibited in the presence of molecular oxygen. A key intermediate in the reactivation process, Ni-SIr, provides the link between fully oxidized (Ni-A, Ni-B) and active (Ni-SIa, Ni-C and Ni-R) forms of hydrogenase. In this work Ni-SIr was found to be light-sensitive (T ≤ 110 K), similar to the active Ni-C and the CO-inhibited states. Transition to the final photoproduct state (Ni-SL) was shown to involve an additional transient light-induced state (Ni-SI1961). Rapid scan kinetic infrared measurements provided activation energies for the transition from Ni-SL to Ni-SIr in protonated as well as in deuterated samples. The inhibitor CO was found not to react with the active site of the Ni-SL state. The wavelength dependence of the Ni-SIr photoconversion was examined in the range between 410 and 680 nm. Light-induced effects were associated with a nickel-centred electronic transition, possibly involving a change in the spin state of nickel (Ni2+). In addition, at T ≤ 40 K the CN− stretching vibrations of Ni-SL were found to be dependent on the colour of the monochromatic light used to irradiate the species, suggesting a change in the interaction of the hydrogen-bonding network of the surrounding amino acids. A possible mechanism for the photochemical process, involving displacement of the oxygen-based ligand, is discussed

The state of health in the European Union (EU-27) in 2019: a systematic analysis for the Global Burden of Disease study 2019

Background: The European Union (EU) faces many health-related challenges. Burden of diseases information and the resulting trends over time are essential for health planning. This paper reports estimates of disease burden in the EU and individual 27 EU countries in 2019, and compares them with those in 2010.Methods: We used the Global Burden of Disease 2019 study estimates and 95% uncertainty intervals for the whole EU and each country to evaluate age-standardised death, years of life lost (YLLs), years lived with disability (YLDs) and disability-adjusted life years (DALYs) rates for Level 2 causes, as well as life expectancy and healthy life expectancy (HALE).Results:In 2019, the age-standardised death and DALY rates in the EU were 465.8 deaths and 20,251.0 DALYs per 100,000 inhabitants, respectively. Between 2010 and 2019, there were significant decreases in age-standardised death and YLL rates across EU countries. However, YLD rates remained mainly unchanged. The largest decreases in age-standardised DALY rates were observed for "HIV/AIDS and sexually transmitted diseases" and "transport injuries" (each -19%). "Diabetes and kidney diseases" showed a significant increase for age-standardised DALY rates across the EU (3.5%). In addition, "mental disorders" showed an increasing age-standardised YLL rate (14.5%).Conclusions: There was a clear trend towards improvement in the overall health status of the EU but with differences between countries. EU health policymakers need to address the burden of diseases, paying specific attention to causes such as mental disorders. There are many opportunities for mutual learning among otherwise similar countries with different patterns of disease

Respiration rates and ethanol and ethylene production of mangoes stored under elevated carbon dioxide and reduced oxigen atmospheres

O objetivo deste trabalho foi avaliar a respiração e produção de etanol e etileno em mangas armazenadas sob reduzidas concentrações de oxigênio (O2) e elevadas concentrações de dióxido de carbono (CO2). Mangas pré-climatéricas das cultivares Kent e Tommy Atkins foram armazenadas, sob fluxo contínuo, por 14 ou 21 dias, a 12°C em três concentrações de O2: 3%, 5% ou 21% em mistura com concentrações de 5%, 15%, 25%, 35%, 45%, 50% ou 70% de CO2. O tratamento-testemunha constou de armazenagem em ar sob fluxo contínuo. Após o período em atmosfera controlada (AC), as mangas foram transferidas e mantidas por 5 dias em ar a 20oC. Mangas sob concentrações de 50% e 70% de CO2 produziram mais etanol que nas demais concentrações de CO2. A redução para 3% de O2 na atmosfera de armazenagem aparentemente não teve efeito adicional ao CO2 na produção de etanol. A atividade respiratória de mangas em AC com CO2 acima de 45% foi muito mais intensa do que a respiração sob as concentrações de CO2 mais baixas; a produção de etileno, no entanto, esteve suprimida, e, mesmo após a transferência para ar, não se recuperou, permanecendo inferior aos demais tratamentos. A queda na atividade respiratória das mangas armazenadas a 50% e 70% de CO2, quando foram transferidas para ar a 20oC indica que as elevadas concentrações de CO2 causaram dano irreparável aos tecidos.The objective of this work was to evaluate respiration rates and ethanol and ethylene production of mangoes stored in controlled atmosphere (CA) with reduced O2 and elevated CO2 concentrations. Pre-climacteric Kent and Tommy Atkins mangoes were stored for 14 or 21 days at 12°C in 5%, 15%, 25%, 35%, 45%, 50% or 70% CO2 plus either 21%, 5% or 3% O2 in a flow through CA system at 12°C. In the control treatment fruits were stored in air at the same temperature and also in the flow through system. After the CA storage the mangoes were transferred and kept for 5 days in air at 20oC. Mangoes stored in either 50% or 70% CO2 had the highest ethanol production rates. The reduction to 3% of the O2 concentration in the storage atmosphere does not seem to have an additional effect on ethanol production. Respiratory rates of mangoes in CA above 45% CO2 atmospheres were higher than in the other treatments, while ethylene production rates were the lowest values. After transfer to air at 20oC, respiration rates of mangoes from these treatments dropped significantly below control levels, which, associated with lower rates of ethylene production, indicate that the mangoes had been damaged by the elevated CO2 atmospheres