Structural insights into the membrane-extracted dimeric form of the ATPase TraB from the Escherichia coli pKM101 conjugation system

Background: Type IV secretion (T4S) systems are involved in secretion of virulence factors such as toxins or transforming molecules, or bacterial conjugation. T4S systems are composed of 12 proteins named VirB1-B11 and VirD4. Among them, three ATPases are involved in the assembly of the T4S system and/or provide energy for substrate transfer, VirB4, VirB11 and VirD4. The X-ray crystal structures of VirB11 and VirD4 have already been solved but VirB4 has proven to be reluctant to any structural investigation so far. Results: Here, we have used small-angle X-ray scattering to obtain the first structural models for the membrane-extracted, dimeric form of the TraB protein, the VirB4 homolog encoded by the E. coli pKM101 plasmid, and for the monomeric soluble form of the LvhB4 protein, the VirB4 homolog of the T4S system encoded by the Legionella pneumophila lvh operon. We have obtained the low resolution structures of the full-length TraB and of its N- and C-terminal halves. From these SAXS models, we derive the internal organisation of TraB. We also show that the two TraB N- and C-terminal domains are independently involved in the dimerisation of the full-length protein. Conclusions: These models provide the first structural insights into the architecture of VirB4 proteins. In particular, our results highlight the modular arrangement and functional relevance of the dimeric-membrane-bound form of TraB

Magnetic Field-Vector Measurements in Quiescent Prominences via the Hanle Effect: Analysis of Prominences Observed at Pic-Du-Midi and at Sacramento Peak

The Hanle effect method for magnetic field vector diagnostics has now provided results on the magnetic field strength and direction in quiescent prominences, from linear polarization measurements in the He I E sub 3 line, performed at the Pic-du-Midi and at Sacramento Peak. However, there is an inescapable ambiguity in the field vector determination: each polarization measurement provides two field vector solutions symmetrical with respect to the line-of-sight. A statistical analysis capable of solving this ambiguity was applied to the large sample of prominences observed at the Pic-du-Midi (Leroy, et al., 1984); the same method of analysis applied to the prominences observed at Sacramento Peak (Athay, et al., 1983) provides results in agreement on the most probable magnetic structure of prominences; these results are detailed. The statistical results were confirmed on favorable individual cases: for 15 prominences observed at Pic-du-Midi, the two-field vectors are pointing on the same side of the prominence, and the alpha angles are large enough with respect to the measurements and interpretation inaccuracies, so that the field polarity is derived without any ambiguity

Stark Broadening of in III Lines in Astrophysical and Laboratory Plasma

Besides the need of Stark broadening parameters for a number of problems in physics, and plasma technology, in hot star atmospheres the conditions exist where Stark widths are comparable and even larger than the thermal Doppler widths. Using the semiclassical perturbation method we investigated here the influence of collisions with charged particles for In III spectral lines. We determined a number of Stark broadening parameters important for the investigation of plasmas in the atmospheres of A-type stars and white dwarfs. Also, we have compared the obtained results with existing experimental data. The results will be included in the STARK-B database, the Virtual Atomic and Molecular Data Center and the Serbian Virtual Observatory

Atomic data and electron-impact broadening effect in DO white dwarf atmospheres: Si VI

Energy levels, electric dipole transition probabilities and oscillator strengths in five times ionized silicon have been calculated in intermediate coupling. The present calculations were carried out with the general purpose atomic-structure program SUPERSTRUCTURE. The relativistic corrections to the non-relativistic Hamiltonian are taken into account through the Breit-Pauli approximation. We have also introduced a semi-empirical correction (TEC) for the calculation of the energy-levels. These atomic data are used to provide semiclassical electron-, proton- and ionized helium- impact line widths and shifts for 15 Si VI muliplet. Calculated results have been used to consider the influence of Stark broadening for DO white dwarf atmospheric conditions.Comment: MNRAS, accepted, 14 page

Stark Broadening of Several Ne II, Ne III and O III Spectral Lines for the STARK-B Database

In order to complete Stark broadening data for Ne II, and O III lines, needed for analysis of stellar atmospheres, we determined, within the semiclassical perturbation method, the missing Stark broadening parameters for the broadening by collisions with protons and ionized helium, for 15 Ne II and 5 O III multiplets. Also, electron, proton, and ionized helium impact broadening parameters for an important Ne II multiplet in the visible part of the spectrum, and for three Ne III multiplets, were calculated. The obtained data will be included in the STARK-B database, which is a part of Virtual Atomic and Molecular Data Center

The Height of Chromospheric Loops in an Emerging Flux Region

Context. The chromospheric layer observable with the He I 10830 {\AA} triplet is strongly warped. The analysis of the magnetic morphology of this layer therefore requires a reliable technique to determine the height at which the He I absorption takes place. Aims. The He I absorption signature connecting two pores of opposite polarity in an emerging flux region is investigated. This signature is suggestive of a loop system connecting the two pores. We aim to show that limits can be set on the height of this chromospheric loop system. Methods. The increasing anisotropy in the illumination of a thin, magnetic structure intensifies the linear polarization signal observed in the He I triplet with height. This signal is altered by the Hanle effect. We apply an inversion technique incorporating the joint action of the Hanle and Zeeman effects, with the absorption layer height being one of the free parameters. Results. The observed linear polarization signal can be explained only if the loop apex is higher than \approx5 Mm. Best agreement with the observations is achieved for a height of 6.3 Mm. Conclusions. The strength of the linear polarization signal in the loop apex is inconsistent with the assumption of a He I absorption layer at a constant height level. The determined height supports the earlier conclusion that dark He 10830 {\AA} filaments in emerging flux regions trace emerging loops.Comment: 7 pages, 4 figure

Stark Broadening of Several Ar I Spectral Lines in the Visible Part of the Spectrum

In order to complete data on Stark broadening parameters for Ar I line in the visible spectrum, we determined Stark widths and shifts due to electron, proton, and ionized helium impacts, for nine lines (�� = 4191.0, 4259.4, 5912.1, 6043.2, 6045.0, 6752.9, 7503.9, 7514.6, 7724.2 °A), using jK coupling and semiclassical-perturbation theory. The obtained results will enter the STARK-B database, which is a part of Virtual Atomic and Molecular Data Center

The human HIP gene, overexpressed in primary liver cancer encodes for a C-type carbohydrate binding protein with lactose binding activity

AbstractHIP was originally identified as a gene expressed in primary liver cancers, and in normal tissues such as pancreas and small intestine. Based on gene data base homologies, the HIP protein should consist of a signal peptide linked to a single carbohydrate recognition domain. To test this hypothesis HIP and the putative carbohydrate recognition domain encoded by the last 138 C-terminal amino acids, were expressed as glutathione-S -transferase proteins (GST-HIP and GST-HIP-142, respectively). Both recombinant proteins were purified by a single affinity purification step from bacterial lysates and their ability to bind saccharides coupled to trisacryl GF 2000M were tested. Our results show that HIP and HIP-142 proteins bind to lactose, moreover the binding requires divalent cations. Thus the HIP protein is a lactose-binding lectin with the characteristics of a C-type carbohydrate recognition domain of 138 amino acids in the C-terminal region