9 research outputs found
Association mapping of seed quality traits using the Canadian flax (Linum usitatissimum L.) core collection
KEY MESSAGE: The identification of stable QTL for seed quality traits by association mapping of a diverse panel of linseed accessions establishes the foundation for assisted breeding and future fine mapping in linseed. ABSTRACT: Linseed oil is valued for its food and non-food applications. Modifying its oil content and fatty acid (FA) profiles to meet market needs in a timely manner requires clear understanding of their quantitative trait loci (QTL) architectures, which have received little attention to date. Association mapping is an efficient approach to identify QTL in germplasm collections. In this study, we explored the quantitative nature of seed quality traits including oil content (OIL), palmitic acid, stearic acid, oleic acid, linoleic acid (LIO) linolenic acid (LIN) and iodine value in a flax core collection of 390 accessions assayed with 460 microsatellite markers. The core collection was grown in a modified augmented design at two locations over 3 years and phenotypic data for all seven traits were obtained from all six environments. Significant phenotypic diversity and moderate to high heritability for each trait (0.73–0.99) were observed. Most of the candidate QTL were stable as revealed by multivariate analyses. Nine candidate QTL were identified, varying from one for OIL to three for LIO and LIN. Candidate QTL for LIO and LIN co-localized with QTL previously identified in bi-parental populations and some mapped nearby genes known to be involved in the FA biosynthesis pathway. Fifty-eight percent of the QTL alleles were absent (private) in the Canadian cultivars suggesting that the core collection possesses QTL alleles potentially useful to improve seed quality traits. The candidate QTL identified herein will establish the foundation for future marker-assisted breeding in linseed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00122-014-2264-4) contains supplementary material, which is available to authorized users
Characterization of novel genic SSR markers in Linum usitatissimum (L.) and their transferability across eleven Linum species
Little is known about the evolutionary relationships among Linum
species, basically because of the lack of transferable molecular
markers. Currently, expressed sequence tags available in public
databases provide an opportunity for the rapid and inexpensive
development of simple sequence repeat (SSR) markers in wild flax
species. In this regard, fifty expressed sequence tag-derived
microsatellite markers (EST-SSRs) were evaluated for polymorphism and
transferability in 50 Linum usitatissimum cultivars/accessions and 11
Linum species. Among them 23 EST-SSRs were polymorphic in L.
usitatissimum, while 2-4 alleles were detected (average 2.26 per
locus). The polymorphism information content value ranged from 0.08 to
0.55 (average 0.38). Forty one genic markers (95.3%) produced strong
amplicons in at least two of the 11 Linum species. The percentage of
cross amplification ranged from 34.1% to 92.7% in L. tauricum and L.
bienne, respectively. Moreover, the rate of transferability was
associated positively with the botanical section. Our results suggest
that the high degree of EST-SSRs transferability to Linum species can
be a useful enhancement of the current database of SSR markers for
future genetic and evolutionary studies
Characterization of novel genic SSR markers in Linum usitatissimum (L.) and their transferability across eleven Linum species
Little is known about the evolutionary relationships among Linum
species, basically because of the lack of transferable molecular
markers. Currently, expressed sequence tags available in public
databases provide an opportunity for the rapid and inexpensive
development of simple sequence repeat (SSR) markers in wild flax
species. In this regard, fifty expressed sequence tag-derived
microsatellite markers (EST-SSRs) were evaluated for polymorphism and
transferability in 50 Linum usitatissimum cultivars/accessions and 11
Linum species. Among them 23 EST-SSRs were polymorphic in L.
usitatissimum, while 2-4 alleles were detected (average 2.26 per
locus). The polymorphism information content value ranged from 0.08 to
0.55 (average 0.38). Forty one genic markers (95.3%) produced strong
amplicons in at least two of the 11 Linum species. The percentage of
cross amplification ranged from 34.1% to 92.7% in L. tauricum and L.
bienne, respectively. Moreover, the rate of transferability was
associated positively with the botanical section. Our results suggest
that the high degree of EST-SSRs transferability to Linum species can
be a useful enhancement of the current database of SSR markers for
future genetic and evolutionary studies
Characterization of novel genic SSR markers in Linum usitatissimum (L.) and their transferability across eleven Linum species
Little is known about the evolutionary relationships among Linum species, basically because of the lack of transferable molecular markers. Currently, expressed sequence tags available in public databases provide an opportunity for the rapid and inexpensive development of simple sequence repeat (SSR) markers in wild flax species. In this regard, fifty expressed sequence tag-derived microsatellite markers (EST-SSRs) were evaluated for polymorphism and transferability in 50 Linum usitatissimum cultivars/accessions and 11 Linum species. Among them 23 EST-SSRs were polymorphic in L. usitatissimum, while 2-4 alleles were detected (average 2.26 per locus). The polymorphism information content value ranged from 0.08 to 0.55 (average 0.38). Forty one genic markers (95.3%) produced strong amplicons in at least two of the 11 Linum species. The percentage of cross amplification ranged from 34.1% to 92.7% in L. tauricum and L. bienne, respectively. Moreover, the rate of transferability was associated positively with the botanical section. Our results suggest that the high degree of EST-SSRs transferability to Linum species can be a useful enhancement of the current database of SSR markers for future genetic and evolutionary studies
Genome-Wide Association Analysis of Mucilage and Hull Content in Flax (Linum usitatissimum L.) Seeds
New flaxseed cultivars differing in seed mucilage content (MC) with low hull content (HC) represent an attractive option to simultaneously target the food and feed markets. Here, a genome-wide association study (GWAS) was conducted for MC and HC in 200 diverse flaxseed accessions genotyped with 1.7 million single nucleotide polymorphism (SNP) markers. The data obtained for MC and HC indicated a broad phenotypic variation and high (~70%) and a moderate (~49%) narrow sense heritability, respectively. MC and HC did not differ statistically between fiber and oil morphotypes, but yellow-seeded accessions had 2.7% less HC than brown-seeded ones. The genome-wide linkage disequilibrium (LD) decayed to r2 = 0.1 at a physical distance of ~100 kb. Seven and four quantitative trait loci (QTL) were identified for MC and HC, respectively. Promising candidate genes identified include Linum usitatissimum orthologs of the Arabidopsis thaliana genes TRANSPARENT TESTA 8, SUBTILISIN-LIKE SERINE PROTEASE, GALACTUROSYL TRANSFERASE-LIKE 5, MUCILAGE-MODIFIED 4, AGAMOUS-LIKE MADS-BOX PROTEIN AGL62, GLYCOSYL HYDROLASE FAMILY 17, and UDP-GLUCOSE FLAVONOL 3-O-GLUCOSYLTRANSFERASE. These genes have been shown to play a role in mucilage synthesis and release, seed coat development and anthocyanin biosynthesis in A. thaliana. The favorable alleles will be useful in flaxseed breeding towards the goal of achieving the ideal MC and HC composition for food and feed by genomic-based breeding