9 research outputs found
CCSReads_DS-PBBC4
Sequel CCS reads for 99%, 99.9%, and 99.99% partitions of data set DS-PBBC4
Data from: A sequel to Sanger: amplicon sequencing that scales
Although high-throughput sequencers (HTS) have largely displaced their Sanger counterparts, the short read lengths and high error rates of most platforms constrain their utility for amplicon sequencing. The present study tests the capacity of single molecule, real-time (SMRT) sequencing implemented on the SEQUEL platform to overcome these limitations, employing 658 bp amplicons of the mitochondrial cytochrome c oxidase I gene as a model system. By examining templates from more than 5,000 species and 20,000 specimens, the performance of SMRT sequencing was tested with amplicons showing wide variation in GC composition and varied sequence attributes. SMRT and Sanger sequences were very similar, but SMRT sequencing provided more complete coverage, especially for amplicons with homopolymer tracts. Because it can characterize amplicon pools from 10,000 DNA extracts in a single run, the SEQUEL reduces costs 40-fold from Sanger analysis. Reflecting the capacity of each instrument to recover sequences from more than five million DNA extracts a year, this platform facilitates massive amplicon characterization
CCSReads_DS-PBBC3
Sequel CCS reads for 99%, 99.9%, and 99.99% partitions of data set DS-PBBC3
CCSReads_DS-PBBC2
Sequel CCS reads for 99%, 99.9%, and 99.99% partitions of data set DS-PBBC2
mBRAVE_DS-PBBC1
mBRAVE output for data set DS-PBBC1
mBRAVE_DS-PBBC3
mBRAVE output for data set DS-PBBC3
CCSReads_DS-PBBC1
Sequel CCS reads for 99%, 99.9%, and 99.99% partitions of data set DS-PBBC1
mBRAVE_DS-PBBC4
mBRAVE output for data set DS-PBBC4