Prospects of veterinary practice in animal productions in France

The current changes in the animal production sector are raising concern among veterinary surgeons working in rural areas about the future of their practices. However, their field of action remains large. To adapt to their changing environment and meet the new expectations of their clients, veterinary surgeons must evolve and offer suitable and economically viable services focusing on a technical and economical approach to animal health. To help practitioners adapt to these new conditions, the French national organisation for veterinary technical groups (SNGTV) launched a strategy designed to provide them with complementary tools and services. This strategy may help them develop their activities, and thus promote the economical development of their practices.Dans un contexte d’évolution des filières de productions animales, les vétérinaires qui exercent en milieu rural se posent des questions sur l’avenir de leurs entreprises. Le champ d’action des praticiens reste large. Pour s’adapter à la transformation de leur environnement professionnel et répondre aux nouvelles attentes de leurs clients, les vétérinaires doivent continuer à évoluer et leur proposer des services adaptés et rentables, dans une approche technico-économique du sanitaire. Pour accompagner les praticiens dans cette mutation professionnelle, la Société nationale des groupements techniques vétérinaires (SNGTV) s’est investie dans une stratégie qui vise à leur fournir des outils et des services complémentaires entre eux. Cette stratégie peut leur permettre de développer leurs activités et, ainsi, de favoriser le développement économique de leurs entreprises

COPRODUCTION DE L’ACTION LOCALE : la legitimite de la decision publique en question

Neste artigo analisa-se a recomposição territorial como parte da definição de uma ação pública local no que concerne ao seu conteúdo, seus objetivos e sua institucionalização. Trata-se de uma contribuição na perspectiva da sociologia e ciência política sobre a ação pública coletiva e organizada. A coprodução é analisada através de unidades sociais portadoras de legitimidade política. O exemplo do Programa de iniciativa Leader conduzido sobre o território chamado Côte sous le vent (Guadeloupe) e em particular o projeto «les jeudis du Clube Med 2 » da prefeitura de Deshaies nos informa sobre modalidades de funcionamento de novas cenas públicas. As configurações que se constroem são baseadas nas noções de identidade, de habitações coloniais, de resistência ao escravismo. O engajamento que se produz pela concertação contribui para a determinação dos ajustamentos necessários à sua conceitualização.Palavras-chave: Ação Pública Local; Identidade; Engajamento; Recomposição Territorial; Guadeloupe.Resume: Cet article porte sur l’analyse de la recom-position territoriale qui participe à la défnition de l’action publique locale dans son contenu, ses objectifs et son institutionnalisation. Il s’agit d’une contribution de la sociologie et de la science politique à la l’action publique en tant qu’action collective et organisée. La coproduction est analysée au travers d’unités sociales porteuses de légitimités politiques. L’exemple du programme d’initiative Leader conduit sur le territoire de la Côte sous le vent (Guadeloupe) et en particulier le projet « les jeudis du club Med2 » de la commune de Deshaies, nous renseigne sur les modalités de fonctionnement des nouvelles scènes publiques. Les confgurations qui se construisent se fondent sur des notions d’identité, d’habitations coloniales, de résistance et de marronnage. L’engagement qui se produit par la concertation participe à la détermination des ajustements nécessaires à sa conceptualisation. Mots clé: Action publique locale; Identité; Engajamento; Recomposition territoriale; Guadeloupe

Collaborations Nord-Sud, TICs et bibliothèques

Co-organisée par la BM de Bordeaux-Mériadeck en collaboration avec l’IUT Michel de Montaigne- Bordeaux 3 (pôle Métiers du livre), l’Agence régionale pour l’écrit et le livre en Aquitaine (Arpel), et l’Agence de médiation culturelle des pays du Sahel, cette journée visait à présenter des exemples de collaboration entre bibliothèques ou institutions éducatives

Apoptotic and chemotherapeutic properties of iron(III)-salophene in an ovarian cancer animal model

The cytotoxicity of organometallic compounds iron(III)-, cobalt(III)-, manganese(II)-, and copper(II)-salophene (-SP) on platinum-resistant ovarian cancer cell lines was compared. Fe-SP displayed selective cytotoxicity (IC 50 at ∼1 μM) against SKOV-3 and OVCAR-3 cell lines while Co-SP caused cytotoxic effects only at higher concentrations (IC50 at 60 ?M) and Cu-SP effects were negligible. High cytotoxicity of Mn-SP (30-60 μM) appeared to be nonspecific because the Mn-chloride salt reduced cell viability similarly. The effect of Fe-SP at 1 μM proved to be ovarian cancer cell selective when compared to a panel of cell lines derived from different tumors. The first irreversible step in the induction of cell death by Fe-SP occurred after 3 hrs as indicated by the mitochondrial transmembrane potential (ΔΨm) and was mainly linked to apoptotic, not necrotic events. To evaluate the toxicity of Fe-SP in vivo we conducted an acute toxicity study in rats. The LD 50 of Fe-SP is >2000 mg/kg orally and >5.5 mg/kg body weight by intraperitoneal injection. An ovarian cancer animal model showed that the chemotherapeutic relevant dose of Fe-SP in rats is 0.5-1 mg/kg body weight. The present report suggests that Fe-SP is a potential therapeutic drug to treat ovarian cancer. © 2009 Lange et al, publisher and licensee Dove Medical Press Ltd

Tetrathiomolybdate sensitizes ovarian cancer cells to anticancer drugs doxorubicin, fenretinide, 5-fluorouracil and mitomycin C

<p>Abstract</p> <p>Background</p> <p>Our recent study showed that tetrathiomolybdate (TM), a drug to treat copper overload disorders, can sensitize drug-resistant endometrial cancer cells to reactive oxygen species (ROS)-generating anticancer drug doxorubicin. To expand these findings in the present study we explore TM efficacy in combination with a spectrum of ROS-generating anticancer drugs including mitomycin C, fenretinide, 5-fluorouracil and doxorubicin in ovarian cancer cells as a model system.</p> <p>Methods</p> <p>The effects of TM alone or in combination with doxorubicin, mitomycin C, fenretinide, or 5-fluorouracil were evaluated using a sulforhodamine B assay. Flow cytometry was used to detect the induction of apoptosis and ROS generation. Immunoblot analysis was carried out to investigate changes in signaling pathways.</p> <p>Results</p> <p>TM potentiated doxorubicin-induced cytotoxicity and modulated key regulators of apoptosis (PARP, caspases, JNK and p38 MAPK) in SKOV-3 and A2780 ovarian cancer cell lines. These effects were linked to the increased production of ROS, as shown in SKOV-3 cells. ROS scavenging by ascorbic acid blocked the sensitization of cells by TM. TM also sensitized SKOV-3 to mitomycin C, fenretinide, and 5-fluorouracil. The increased cytotoxicity of these drugs in combination with TM was correlated with the activity of ROS, loss of a pro-survival factor (e.g. XIAP) and the appearance of a pro-apoptotic marker (e.g. PARP cleavage).</p> <p>Conclusions</p> <p>Our data show that TM increases the efficacy of various anticancer drugs in ovarian cancer cells in a ROS-dependent manner.</p

Organometallic Iron(III)-Salophene Exerts Cytotoxic Properties in Neuroblastoma Cells via MAPK Activation and ROS Generation

The objective of the present study was to investigate the specific effects of Iron(III)-salophene (Fe-SP) on viability, morphology, proliferation, cell cycle progression, ROS generation and pro-apoptotic MAPK activation in neuroblastoma (NB) cells. A NCI-DTP cancer screen revealed that Fe-SP displayed high toxicity against cell lines of different tumor origin but not tumor type-specificity. In a viability screen Fe-SP exhibited high cytotoxicity against all three NB cell lines tested. The compound caused cell cycle arrest in G1 phase, suppression of cells progressing through S phase, morphological changes, disruption of the mitochondrial membrane depolarization potential, induction of apoptotic markers as well as p38 and JNK MAPK activation, DNA degradation, and elevated generation of reactive oxygen species (ROS) in SMS-KCNR NB cells. In contrast to Fe-SP, non-complexed salophene or Cu(II)-SP did not raise ROS levels in NB or SKOV-3 ovarian cancer control cells. Cytotoxicity of Fe-SP and activation of caspase-3, -7, PARP, pro-apoptotic p38 and JNK MAPK could be prevented by co-treatment with antioxidants suggesting ROS generation is the primary mechanism of cytotoxic action. We report here that Fe-SP is a potent growth-suppressing and cytotoxic agent for in vitro NB cell lines and, due to its high tolerance in previous animal toxicity studies, a potential therapeutic drug to treat NB tumors in vivo