Physiological responses of a hyper-phototropic mutant to various light stimuli [abstract]

Abstract only availableFaculty Mentor: Mannie Liscum, Biological SciencesPhysiological responses of a hyper-phototropic mutant to various light stimuli Phototropic responses are the directional curvature of organs in response to differences in light intensity and/or quality. The experimental plant is the hyper-phototropic hypocotyl () mutant that is associated specifically with phototropism and is hyper-responsive to blue light stimulation. In wild-type seedlings the response is dependent upon the fluence of blue light (number of incident of photons) used to stimulate seedlings. In this analysis seedlings were exposed to several fluence rates of blue light. Results from these experiments suggest that the phenotypes are specific to lower fluence rates. Additionally, red light which has been shown to enhance the phototropic response to blue light was used as a pretreatment to assess whether is altered in its response to red light as well as blue. Results from this latter analysis show that mutant seedling still exhibit a higher degree of curvature than control samples, suggesting the red light enhances the phototropic response of as well. Together these experiments suggest that affects a repressor of phototropin 1-dependent phototropism in low blue light conditions.University of Missouri--Columbia. Office of Undergraduate ResearchPlant Genomics Internship @ M

Natural variation in phenotypes associated with phototropins among geographically isolated populations of Arabidopsis thaliana [abstract]

Abstract only availableIn Arabidopsis thaliana several phenotypes are controlled by blue light including chloroplast relocation movements and bending of the seedling stem in phototropism. These responses are controlled by the phototropins, phot1 and phot2. Chloroplasts move towards the brightest area in a plant cell under low light intensity to maximize light interception (accumulation response), but toward darker areas under high intensity light to minimize photo-damage (avoidance response). Phot1 and phot2 control the accumulation movement, but phot2 alone controls the avoidance response. Phototropism in response to weak blue light is controlled solely by phot1. Here, we have tested seventeen different accessions of A. thaliana for phenotypes uniquely associated with phot1 and phot2 and examined variation in phot1 and phot2 sequence data for a small subset. Accessions were chosen to better understand the ecological context of variation in phototropin function in nature.National Science Foundation grant to M. Liscu

Aportes de la educación musical a las y los estudiantes con necesidades educativas especiales: un estudio de caso de un egresado de la carrera de Educación Musical de la Universidad Andrés Bello en la “Escuela Especial de Desarrollo” de la comuna La Reina, Santiago de Chile

Tesis (Profesor de Educación Musical para la Educación Preescolar y Básica, Licenciado en Educación)El presente estudio pretende analizar la concordancia entre la formación profesional de un profesor de música y las estrategias utilizadas para favorecer el aprendizaje de estudiantes en situación de discapacidad intelectual que asisten a un establecimiento especial. Esto se desarrolló desde agosto hasta noviembre del 2018. Se han tomado en cuenta las disciplinas que convergen en torno a la Educación Especial, tales como la Terapia Ocupacional, la Psicopedagogía, la Educación Diferencial, la Educación Musical y la Musicoterapia. También, se consideraron las legislaciones y normativas que rigen este sistema educativo. Esto se sustentó en un paradigma constructivista, con una metodología de carácter cualitativa. Se aplicó una entrevista en profundidad y se observaron clases impartidas por el docente de este estudio de caso. A partir de la triangulación de los resultados obtenidos, es posible inferir que los y las docentes de Educación Musical no poseen las estrategias suficientes para desenvolverse en un contexto educativo especial, específicamente por parte de la formación académica, sino que es resultado de la intuición y experiencia pedagógica personal de cada docente. A modo de conclusión, se ha invitado a seguir investigando la incidencia de la Educación Musical en la Educación Especial, con el fin de crear una red de conocimientos compartidos en torno a esta temática. Además, se ha hecho una propuesta que se espera, sirva de apoyo pedagógico para quién la requieraThe following study analyzes the relation between the professional training of a music professor and the strategies used to encourage student learning in a situation of intellectual disability in a special establishment. This study was performed between August and November 2018. The relevant disciplines that converge around Special Education have been taken into account in this study, such as occupational therapy, psycho pedagogy, differential education, musical education and music therapy. The legislation and norms that govern the educational system, have been considered. This study was carried out with a constructivist paradigm and with a qualitative methodology. The case study includes an in-depth interview and observations of the teacher in the classroom. Throughout the triangulation of the results obtained, conclude that teachers of musical education do not have the sufficient strategies to perform in a context of special educative needs. Specifically, these strategies are absent from academic formation, but result from the intuition and pedagogic experience of each teacher. In conclusion, there is an invitation to continue researching the incidence of music education on special education, with the goal of creating a network of shared knowledge around this topic. In addition, a proposal has been made that is expected to serve as pedagogical support for whoever may require it

Investigation of Coarse Aggregate Strength for Use in Stone Matrix Asphalt

Stone Matrix Asphalt is a gap-graded hot-mix asphalt mixture composed of a coarse aggregate skeleton and a binder-rich mortar. The mixture type was first introduced to the United States in 1991, with one of the first test sections placed on I-70 near Richmond, Indiana. To help control the selection of coarse aggregate, the Indiana Department of Transportation specified a maximum Los Angeles Abrasion loss value of 30 percent. An investigation into the coarse aggregate specifications for use in Stone Matrix Asphalt was completed in this study. Emphasis was placed on evaluating various tests that may be useful is specifying coarse aggregates, and to develop a test or set of tests and specifications. Finally, the validity of the current 30 percent Los Angeles Abrasion loss value as requirement for coarse aggregate selection was determined. A survey of state agencies revealed a large variation in the Los Angeles Abrasion values currently specified. Laboratory testing revealed that the Micro-Deval test is a good compliment to the Los Angeles Abrasion test. The Micro- Deval test presents an added benefit as it includes the presence of water. Of the four tests investigated, aggregate degradation during compaction was the most accurate method for predicting coarse aggregate performance of the four tests. A combination of the Los Angeles Abrasion, Micro-Deval, and aggregate degradation tests was even more accurate in predicting coarse aggregate performance

Insights into phototropic signaling : a study of the unusual attributes of a hyper-phototropic mutant

Title from PDF of title page (University of Missouri--Columbia, viewed on November 22, 2010).The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.Dissertation advisor: Dr. Emmanuel Liscum III.Vita.Ph. D. University of Missouri--Columbia 2008.[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Many questions persist about the nature of and components associated with the initial signaling events that follow light perception for phototropism. To address this, we began by describing a hyper-phototropic mutant -- hph, through a series of directed experiments. The primary goal of this research was to gain a deeper understanding of the phototropic signal progression from stimulus to response. While the original hph mutant represents an interesting physiological aberration, only following our analysis was the discovery that hph could offer additional insights made possible. To begin, we considered the physiological and genetic basis for the hph phenotype (Ch. 2). Based on these discoveries we then began to address the molecular aspects of hph through the use of transgenic plants and, subsequently, other tools to decipher the proteinprotein interactions (Ch. 3). To conclude, we have initiated an ecological study focused on establishing a linkage between phototropin action and ecological fitness and success (Ch. 4).Includes bibliographical references (p. 147-168)

Enhancement of plant vision to increase drought tolerance and bioproduction [abstract]

Only abstract of poster available.Track V: BiomassEnergy needs of the worlds growing population have become central issues to policy and science discussion over the past few years. Not only are our non-renewable sources of energy being depleted at alarming rates, their consumption is also a major contributor to the accumulation of atmospheric greenhouse gases. It is clear that new solutions to old problems must be found, and in this context the production and harnessing of biofuel products holds great promise. Plant-based bioproduction has the great advantage that biofuel production can be creatively couple with food production, another pressing 21st Century issue. Water availability represents the major limitation to increased plant-based production, both in the U.S. and around the world. Therefore development of plants that are better able to access and utilize this limiting resource is paramount. Our studies in the model plant Arabidopsis thaliana have shown that mutants lacking the key photoreceptor protein, phototropin 1 (phot1), that mediates a plants response to directional blue light fail to orient their root growth properly and thus exhibit increased drought susceptibility (Galen et al. 2004, 2007). Conversely, mutations in phot1 that confer increased responsiveness to blue light appear to increase drought tolerance. We are now exploring ways to recapitulate this exciting phenotype through the generation of GMOs, both in this model species and in crop plants. This approach holds great potential as even minor increases in drought tolerance in plants can result in dramatic increases in bioproduction, the ultimate goal of a plant-based biofuels industry

The diurnal oscillation of drought-responsive genes in soybean leaves during moderate stress is confirmed by RNA-seq analysis.

<p>Collect time points are represented by ZT (Zeitgeiber Time) 0 to 20, starting from the time the lights came on ( ZT0) and proceeding with 4 h intervals until ZT20. The error bars represent the standard error. The ANOVA and the Tukey HSD (95% family-wise confidence level) multiple comparison tests can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086402#pone.0086402.s007" target="_blank">Table S4</a>.</p

Drought-responsive genes in soybean exhibit diurnal regulation, and the expression pattern is modified under drought stress.

<p>Gene expression data regards qPCR analysis of soybean leaves during moderate (A) and severe (B) drought. Expression axis represents normalized expression (NE) = 2∧^{-(Ct experimental – Ctn)}. Collect time points are represented by ZT (Zeitgeiber Time) 0 to 20, starting from the time the lights came on ( ZT0) and proceeding with 4 h intervals until ZT20. For easy viewing, asterisks represent significant differences between control and stressed plants in each time point (Duncan’ test 5%, <i>time -treatment</i> interaction). The ANOVA and the complete Duncan’s tests results can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086402#pone.0086402.s006" target="_blank">Table S3</a>.</p

ABA treatment affects the regulation of drought-responsive and some circadian clock genes.

<p>Gene expression data regards qPCR analysis of circadian clock (A) and drought-responsive (B) genes. Expression axis represents normalized expression (NE) = 2∧^{-(Ct experimental – Ctn)}. Collect time points are represented by ZT (Zeitgeiber Time) 4 to 24, starting 4h after the lights came on ( ZT4) and proceeding with 4 h intervals until ZT24. For easy viewing, asterisks represent significant differences between control and stressed plants in each time point (Duncan’s test 5%, <i>time -treatment</i> interaction). The ANOVA and the complete Duncan’s tests results can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086402#pone.0086402.s008" target="_blank">Table S5</a>.</p