761 research outputs found
Inhibition of inducible Nitric Oxide Synthase expression by an acetonic extract from Feijoa sellowiana Berg. fruits.
Feijoa sellowiana Berg. fruits and especially the acetonic extract have been shown to possess biological activities, although the responsible compounds have never been identified. The present study was designed to evaluate the anti-inflammatory activity of an acetonic extract from F. sellowiana Berg. fruits on the nitric oxide (NO) pathway, which plays an important role in inflammation. To this aim the J774 cell line, which expresses inducible nitric oxide synthase (iNOS) following stimulation with lipopolysaccharide (LPS), has been utilized, and the effects of this extract and its fractions on NO production, iNOS protein expression, and signal pathways involved in its regulation have been evaluated. This study demonstrates that at least some part of the anti-inflammatory activity of the acetonic extract is due to the suppression of NO production by flavone and stearic acid. The mechanism of this inhibition seems to be related to an action on the expression of the enzyme iNOS through the attenuation of nuclear factor κB (NF-κB) and/or mitogen-activated protein kinase (MAPK) activation
Parabolic oblique derivative problem in generalized Morrey spaces
We study the regularity of the solutions of the oblique derivative problem
for linear uniformly parabolic equations with VMO coefficients. We show that if
the right-hand side of the parabolic equation belongs to certain generalized
Morrey space than the strong solution belongs to the corresponding generalized
Sobolev-Morrey space
Dynamical response of the "GGG" rotor to test the Equivalence Principle: theory, simulation and experiment. Part I: the normal modes
Recent theoretical work suggests that violation of the Equivalence Principle
might be revealed in a measurement of the fractional differential acceleration
between two test bodies -of different composition, falling in the
gravitational field of a source mass- if the measurement is made to the level
of or better. This being within the reach of ground based
experiments, gives them a new impetus. However, while slowly rotating torsion
balances in ground laboratories are close to reaching this level, only an
experiment performed in low orbit around the Earth is likely to provide a much
better accuracy.
We report on the progress made with the "Galileo Galilei on the Ground" (GGG)
experiment, which aims to compete with torsion balances using an instrument
design also capable of being converted into a much higher sensitivity space
test.
In the present and following paper (Part I and Part II), we demonstrate that
the dynamical response of the GGG differential accelerometer set into
supercritical rotation -in particular its normal modes (Part I) and rejection
of common mode effects (Part II)- can be predicted by means of a simple but
effective model that embodies all the relevant physics. Analytical solutions
are obtained under special limits, which provide the theoretical understanding.
A simulation environment is set up, obtaining quantitative agreement with the
available experimental data on the frequencies of the normal modes, and on the
whirling behavior. This is a needed and reliable tool for controlling and
separating perturbative effects from the expected signal, as well as for
planning the optimization of the apparatus.Comment: Accepted for publication by "Review of Scientific Instruments" on Jan
16, 2006. 16 2-column pages, 9 figure
Myrtucommulone from Myrtus communis exhibits potent anti-inflammatory effectiveness in vivo.
Myrtucommulone a nonprenylated acylphloroglucinol contained in the leaves of myrtle (Myrtus communis), has been reported to suppress the biosynthesis of eicosanoids by inhibition of 5-lipoxygenase and cyclooxygenase-1 in vitro and to inhibit the release of elastase and the formation of reactive oxygen species in activated polymorphonuclear leukocytes. Here, in view of the ability of MC to suppress typical proinflammatory cellular responses in vitro, we have investigated the effects of MC in in vivo models of inflammation. MC was administered to mice intraperitoneally, and paw edema and pleurisy were induced by the subplantar and intrapleural injection of carrageenan, respectively. MC (0.5, 1.5, and 4.5 mg/kg i.p.) reduced the development of mouse carrageenan-induced paw edema in a dose-dependent manner. Moreover, MC (4.5 mg/kg i.p. 30 min before and after carrageenan) exerted anti-inflammatory effects in the pleurisy model. In particular, 4 h after carrageenan injection in the pleurisy model, MC reduced: 1) the exudate volume and leukocyte numbers; 2) lung injury (histological analysis) and neutrophil infiltration (myeloperoxidase activity); 3) the lung intercellular adhesion molecule-1 and P-selectin immunohistochemical localization; 4) the cytokine levels (tumor necrosis factor-α and interleukin-1 β in the pleural exudate and their immunohistochemical localization in the lung; 5) the leukotriene B 4, but not prostaglandin E2, levels in the pleural exudates; and 6) lung peroxidation (thiobarbituric acid-reactant substance) and nitrotyrosine and poly (ADP-ribose) immunostaining. In conclusion, our results demonstrate that MC exerts potent anti-inflammatory effects in vivo and offer a novel therapeutic approach for the management of acute inflammation. Copyright © 2009 by The American Society for Pharmacology and Experimental Therapeutics
COVID-19 and individual genetic susceptibility/receptivity: Role of ACE1/ACE2 genes, immunity, inflammation and coagulation. might the double x-chromosome in females be protective against SARS-COV-2 compared to the single x-chromosome in males?
In December 2019, a novel severe acute respiratory syndrome (SARS) from a new coronavirus (SARS-CoV-2) was recognized in the city of Wuhan, China. Rapidly, it became an epidemic in China and has now spread throughout the world reaching pandemic proportions. High mortality rates characterize SARS-CoV-2 disease (COVID-19), which mainly affects the elderly, causing unrestrained cytokines-storm and subsequent pulmonary shutdown, also suspected micro thromboembolism events. At the present time, no specific and dedicated treatments, nor approved vaccines, are available, though very promising data come from the use of anti-inflammatory, anti-malaria, and anti-coagulant drugs. In addition, it seems that males are more susceptible to SARS-CoV-2 than females, with males 65% more likely to die from the infection than females. Data from the World Health Organization (WHO) and Chinese scientists show that of all cases about 1.7% of women who contract the virus will die compared with 2.8% of men, and data from Hong Kong hospitals state that 32% of male and 15% of female COVID-19 patients required intensive care or died. On the other hand, the long-term fallout of coronavirus may be worse for women than for men due to social and psychosocial reasons. Regardless of sex-or gender-biased data obtained from WHO and those gathered from sometimes controversial scientific journals, some central points should be considered. Firstly, SARS-CoV-2 has a strong interaction with the human ACE2 receptor, which plays an essential role in cell entry together with transmembrane serine protease 2 (TMPRSS2); it is interesting to note that the ACE2 gene lays on the X-chromosome, thus allowing females to be potentially heterozygous and differently assorted compared to men who are definitely hemizygous. Secondly, the higher ACE2 expression rate in females, though controversial, might ascribe them the worst prognosis, in contrast with worldwide epidemiological data. Finally, several genes involved in inflammation are located on the X-chromosome, which also contains high number of immune-related genes responsible for innate and adaptive immune responses to infection. Other genes, out from the RAS-pathway, might directly or indirectly impact on the ACE1/ACE2 balance by influencing its main actors (e.g., ABO locus, SRY, SOX3, ADAM17). Unexpectedly, the higher levels of ACE2 or ACE1/ACE2 rebalancing might improve the outcome of COVID-19 in both sexes by reducing inflammation, thrombosis, and death. Moreover, X-heterozygous females might also activate a mosaic advantage and show more pronounced sex-related differences resulting in a sex dimorphism, further favoring them in counteracting the progression of the SARS-CoV-2 infection
Generalized Jacobi identities and ball-box theorem for horizontally regular vector fields
We consider a family of vector fields and we assume a horizontal regularity
on their derivatives. We discuss the notion of commutator showing that
different definitions agree. We apply our results to the proof of a ball-box
theorem and Poincar\'e inequality for nonsmooth H\"ormander vector fields.Comment: arXiv admin note: material from arXiv:1106.2410v1, now three separate
articles arXiv:1106.2410v2, arXiv:1201.5228, arXiv:1201.520
Self-chemisorption of azurin on functionalized oxide surfaces for the implementation of biomolecular devices
Abstract In this work, we investigate the formation of redox protein Azurin (Az) monolayers on functionalized oxygen exposing surfaces. These metallo-proteins mediate electron transfer in the denitrifying chain of Pseudomonas bacteria and exhibit self-assembly properties, therefore they are good candidates for bio-electronic applications. Azurin monolayers are self-assembled onto silane functionalized surfaces and characterized by atomic force microscopy (AFM). We show also that a biomolecular field effect transistor (FET) in the solid state can be implemented by interconnecting an Azurin monolayer immobilized on SiO 2 with two gold nanoelectrodes. Transport experiments, carried out at room temperature and ambient pressure, show FET behavior with conduction modulated by the gate potential
Recovery of a Medieval Brucella melitensis genome using shotgun metagenomics
ABSTRACT Shotgun metagenomics provides a powerful assumption-free approach to the recovery of pathogen genomes from contemporary and historical material. We sequenced the metagenome of a calcified nodule from the skeleton of a 14th-century middle-aged male excavated from the medieval Sardinian settlement of Geridu. We obtained 6.5-fold coverage of a Brucella melitensis genome. Sequence reads from this genome showed signatures typical of ancient or aged DNA. Despite the relatively low coverage, we were able to use information from single-nucleotide polymorphisms to place the medieval pathogen genome within a clade of B. melitensis strains that included the well-studied Ether strain and two other recent Italian isolates. We confirmed this placement using information from deletions and IS711 insertions. We conclude that metagenomics stands ready to document past and present infections, shedding light on the emergence, evolution, and spread of microbial pathogens
Basic properties of nonsmooth Hormander's vector fields and Poincare's inequality
We consider a family of vector fields defined in some bounded domain of R^p,
and we assume that they satisfy Hormander's rank condition of some step r, and
that their coefficients have r-1 continuous derivatives. We extend to this
nonsmooth context some results which are well-known for smooth Hormander's
vector fields, namely: some basic properties of the distance induced by the
vector fields, the doubling condition, Chow's connectivity theorem, and, under
the stronger assumption that the coefficients belong to C^{r-1,1}, Poincare's
inequality. By known results, these facts also imply a Sobolev embedding. All
these tools allow to draw some consequences about second order differential
operators modeled on these nonsmooth Hormander's vector fields.Comment: 60 pages, LaTeX; Section 6 added and Section 7 (6 in the previous
version) changed. Some references adde
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